• Title/Summary/Keyword: variety identification

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A Study on the Computer Assisted Dental Identification in Mass Disaster (대형참사시 컴퓨터를 이용한 법의치과학적 개인식별)

  • Shin, Kyoung-Jin;Choi, Jong-Hoon;Yoon, Chang-Lyuk;Kim, Chong-Youl
    • Journal of Oral Medicine and Pain
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    • v.24 no.1
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    • pp.81-94
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    • 1999
  • This study intends to find usefulness of the computer assisted dental identification in mass disaster. The variety of dental characteristics was investigated through the research of dental records of 508 adults. And a computer assisted simulation program was used to evaluate the selectivity of dental identification. Findings were as follows : 1. Combinations of dental characteristics were found 155 types. The most various dental characteristic was showed on the mandibular first molar. 99.0% of subjects had dental characteristics for dental identification. 2. The posterior teeth, in comparison with anterior teeth, showed higher selectivity in dental identification which was enhanced by information on the material, type and cavity of the restoration. 3. The variety of dental characteristics was mainly found on the combinations of missing tooth with the material, type and cavity of the restoration. 4. The computer assisted dental identification program, with informa- tion about one's tooth state, made individual identification possible when there was only a part of the teeth in a corpse. 5. The computer assisted dental identification had considerably high selectivity based on the variety of dental characteristics. And it was also performed faster and preciser than the existing identification methods. Based on the results of this study, there are various combinations of the feature of the tooth itself with dental characteristics caused by a certain type of treatment on the teeth. And using the computer assisted dental identification program based on this, dental identification can be more efficient economically and more useful than any other forensic identification methods.

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Use of Simple Sequence Repeat (SSR) Markers for Variety Identification of Tomato (Lycopersicon esculentum) (Simple Sequence Repeat (SSR) Marker를 이용한 토마토 품종 식별)

  • Kwon, Yong-Sham;Park, Eun-Kyung;Bae, Kyung-Mi;Yi, Seung-In;Park, Soon-Gi;Cho, Il-Ho
    • Journal of Plant Biotechnology
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    • v.33 no.4
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    • pp.289-295
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    • 2006
  • This study was carried out to evaluate the suitability of simple sequence repeat (SSR) markers for varietal identification and genetic diversity in 28 commercial tomato varieties. The relationship between marker genotypes and 28 varieties was analyzed. Of the 219 pairs of SSR primers screened against ten tomato varieties, 18 pairs were highly polymorphic with polymorphism information content (PIC) ranging from 0.467 to 0.800. Among the polymorphic loci, two to nine SSR alleles were detected for each locus with an average of 3.3 alleles per locus. Genetic distances were estimated according to Jaccard's methods based on the probability that the amplified fragment from one genotype would be present in another genotype. These varieties were categorized into cherry and classic fruit groups corresponding to varietal types and genetic distance of cluster ranging from 0.35 to 0.97. The phonogram discriminated all varieties by marker genotypes. The SSR markers proved to be useful variety identification and genetic resource analysis of tomato.

Identification of Rice Variety Using Simple Sequence Repeat (SSR) Marker (Simple sequence repeat (SSR) marker를 이용한 벼 품종 식별)

  • Kwon, Yong-Sham;Park, Eun-Kyung;Park, Chan-Ung;Bae, Kyung-Mi;Yi, Seung-In;Cho, Il-Ho
    • Journal of Life Science
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    • v.16 no.6
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    • pp.1001-1005
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    • 2006
  • The objective of this study was carried out to evaluate the suitability of simple sequence repeat (SSR) markers for genetic diversity assessment and identification of rice varieties. The 23 primers selected from 50 SSR primers showed polymorphisms in the 21 rice varieties. The 2 to 9 SSR alleles were detected for each locus with an average of 3.00 alleles per locus. The polymorphism information content (PIC) ranged form 0.091 to 0.839. Based on band patterns, UPGMA cluster analysis was conducted. These varieties were separate into 4 groups corresponding to rice ecotype and pedigree information and genetic distance of cluster ranging from 0.59 to 0.92. The 4 SSR primer sets (RM206, RM225, RM418, RM478) selected form 23 polymorphic primers were differentiated all the rice variety from each other by markers genotypes. These markers may be used wide range of practical application in variety identification of rice.

Analysis of Genetic Relationship of Apple Varieties using Microsatellite Markers (Microsatellite 마커를 이용한 사과 품종 간 유전적 유연관계 분석)

  • Hong, Jee-Hwa;Kwon, Yong-Sham;Choi, Keun-Jin
    • Journal of Life Science
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    • v.23 no.6
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    • pp.721-727
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    • 2013
  • The objective of this study was to evaluate the suitability of microsatellite markers for variety identification in 42 apple varieties. For microsatellite analysis, 305 primer pairs were screened in 8 varieties and twenty six primer pairs showed polymorphism with clear band pattern and repetitive reproducibility. A total of 165 polymorphic amplified fragments were obtained in 42 varieties using 26 markers. Two to twelve alleles were detected for each locus with an average of 6.4 alleles per locus. A value of polymorphism information content (PIC) ranged from 0.461 to 0.849 with an average of 0.665. A total of 165 marker loci were used to calculate Jaccard's distance coefficients using unweighted pair-group method with arithmetical average (UPGMA) cluster analysis. Genetic distance of cluster ranged from 0.27 to 1.00. Analysis of genetic relationship revealed that these 26 microsatellite marker sets discriminated a total of 41 varieties except for 1 variety among 42 varieties. These markers will be utilized as molecular data in variety identification of apple.

Genetic Diversity of Korean Barley (Hordeum vulgare L.) Varieties Using Microsatellite Markers (Microsatellite 마커를 이용한 한국 보리 품종의 유전적 다양성)

  • Kwon, Yong-Sham;Hong, Jee-Hwa;Choi, Keun-Jin
    • Korean Journal of Breeding Science
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    • v.43 no.4
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    • pp.322-329
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    • 2011
  • Microsatellite markers were utilized to investigate genetic diversity among 70 Korean barley varieties (Hordeum vulgare). Ninety nine microsatellite primer pairs were screened for 9 varieties. Twenty primer pairs showed highly polymorphic. The relationship between markers genotypes and 70 varieties was analyzed. A total of 124 polymorphic amplified fragments were obtained by using 20 microsatellite markers. Two to nine SSR alleles were detected for each locus with an average of 6.2 alleles per locus. Average polymorphism information content (PIC) was 0.734, ranging from 0.498 to 0.882. A total of 124 marker loci were used to calculate Jaccard's distance coefficients for cluster analysis using UPGMA. Clustering group was divided 2 groups corresponding to 2-rowed and 6-rowed barley varieties. The phenogram was discriminated all varieties by markers genotypes. These markers may be used wide range of practical application in variety identification and genetic purity assessment of barley.

Construction of a Microsatellite Marker Database of Commercial Pepper Cultivars (유통 중인 고추 품종에 대한 Microsatellite 마커 Data Base 구축)

  • Kwon, Yong-Sham;Hong, Jee-Hwa;Choi, Keun-Jin
    • Horticultural Science & Technology
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    • v.31 no.5
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    • pp.580-589
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    • 2013
  • This study was carried out to evaluate the suitability of microsatellite markers for varietal identification and genetic relationship of 170 commercial pepper cultivars. The relationship between marker genotypes and 11 pepper cultivars with different morphological traits was also analyzed. Of the 302 pairs of microsatellite primers screened against 11 pepper cultivars, 24 pairs were highly polymorphic in terms of number of alleles. These markers were applied for the construction of DNA profile data base for 170 commercial pepper cultivars. A total of 164 polymorphic amplified fragments were obtained from 24 microsatellite primers. The average polymorphism information content was 0.673 ranging from 0.324 to 0.824. One hundred and sixty four microsatellite alleles were used to calculate Jaccard's distance coefficients using unweighted pair group method. A clustering group of varieties, based on the results of microsatellite analysis, were categorized into 3 major groups corresponding to morphological traits. The phenogram discriminated all varieties by markers genotypes. These microsatellite markers will be useful as a tool for protection of plant breeders' intellectual property rights through variety identification in distinctness, uniformity and stability test.

IDENTIFICATION OF GEOGRAPHICAL ORIGIN AND VARIETY OF GREEN COFFEE BY NIR

  • Nzabonimpa, Rukundo;Prodolliet, Jacques;Vouilloz, Annick
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.1151-1151
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    • 2001
  • The international coffee trade is conducted almost exclusively with green coffee. The main coffee producing countries include Brazil, Columbia, Indonesia, Mexico and the Ivory Coast. About 99 % of the coffee grown throughout the world belong to two coffee plant varieties that are commonly known as Arabica and Robusta. The quality of green coffee can be assessed according to several ISO standards (1,2,3,4,5). However, no official international standards for the authenticity of green coffee have been issued. It is important to know the country of origin of the coffee for the purposes of fair international trade. The geographic origin of the coffee is often stated on the label of coffee products such as speciality roasted and soluble coffees. Near Infrared Spectroscopy (NIR) is an accepted technique for quantitative analysis of various parameters in routine QC analysis of food products. It would appear to be a promising candidate as a tool for identification of green coffee origin and numerous feasibility studies have appeared in the literature on its use for soluble, roasted and green coffee variety identification as well as identification of arabica or robusta coffees. NIR spectrophotometers when configured in the reflectance mode are able to perform a complete profile of the NIR spectrum on whole beans. The data can then be interpreted by discriminant chemometrics data analysis. This is the approach used in the present study.

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