• Journal of Microbiology and Biotechnology
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• v.22 no.2
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• pp.234-238
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• 2012

Recombinant Escherichia coli displaying organophosphorus hydrolase (OPH) was used to overcome the diffusion barrier limitation of organophosphorus pesticides. A new anchor system derived from the N-terminal domain of ice-nucleation protein from Pseudomonas syringae InaV (InaV-N) was used to display OPH onto the surface. The designed sequence was cloned in the vector pET-28a(+) and then was expressed in E. coli. Tracing of the expression location of the recombinant protein using SDS-PAGE showed the presentation of OPH by InaV-N on the outer membrane, and the ability of recombinant E. coli to utilize diazinon as the sole source of energy, without growth inhibition, indicated its significant activity. The location of OPH was detected by comparing the activity of the outer membrane fraction with the inner membrane and cytoplasm fractions. Studies revealed that recombinant E. coli can degrade 50% of 2 mM chlorpyrifos in 2 min. It can be concluded that InaV-N can be used efficiently to display foreign functional protein, and these results highlight the high potential of an engineered bacterium to be used in bioremediation of pesticide-contaminated sources in the environment.

• Molecules and Cells
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• v.24 no.1
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• pp.125-131
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• 2007

von Willebrand factor (vWF) is a multimeric glycoprotein which functions within the coagulation system. It colocalizes with factor VIII (FVIII) by non-covalent interaction and alters its intracellular trafficking. vWF is also instrumental in maintaining the stability of secreted FVIII. The principal objective of this study was to generate a lentivirus-based vWF expression vector for gene therapy of hemophilia A. We inserted a vWF of 8.8 Kb into a lentiviral vector thereby producing VSV-G-pseudotyped vEx52. However, its titer was quite low, presumably because the length of vWF gene exceeds the size limit of the lentiviral vector. In order to overcome the low-titer, we concentrated the vEx52 and thus increased the efficiency of transduction approximately 6-fold with $1/100^{th}$ of the volume. However, as concentration requires an additional laborious step, we attempted to enhance the transduction efficiency by deleting exons 24-46 and 29-46 in pRex52 to construct pRex23 and pRex28, and in pvEx52, yielding pvEx23 and pvEx28, respectively. The transfected pRex52 had a profound effect on the activity of secreted FVIII, and this activity declined as domains of vWF were deleted. However, when the domain-deleted vWF-lentiviruses were transduced into K562 cells, the vEx28 increased the activity of the secreted FVIII compared to what was observed with vEx52. This result is probably due to higher efficiencies of transduction and expression while retaining the essential domains required for proper interaction with FVIII.

• Journal of Arbitration Studies
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• v.13 no.2
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• pp.519-546
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• 2004

On June 27, 2002 Canadian Internet Registration Authority (CIRA) launched dot-ca domain name dispute resolution service through BCICAC and Resolution Canada, Inc. The Domain name Dispute Resolution Policy (CDRP) of CIRA is basically modelled after Uniform Domain Name Dispute Resolution Policy(UDRP), while the substance of CDRP is slightly modified to meet the need of Canadian domain name regime and its legal system. Firstly, this article examined CIRA's domain name dispute resolution policy in general. It is obvious that the domain name dispute resolution proceeding is non-binding arbitration to which arbitration law does not apply. However it still belongs to the arbitration and far from the usual mediation process. Domain name arbitrators render decision rather than assist disputing parties themselves reach to agreement. In this respect the domain name arbitration is similar to arbitration or litigation rather than mediation. Secondly it explored how the panels applied the substantive standards in domain name arbitration. There is some criticism that panelists interprets the test of "confusingly similar" in conflicting manner. As a result critics assert that courts' judicial review is necessary to reduce the conflicting interpretation on the test of substantive standards stipulated in paragraph 3 of CDRP. Finally, it analysed the court's position on domain name arbitral award. Canadian courts do not seem to establish a explicit standard for judicial review over it yet. However, in Black v. Molson case Ontario Superior Court applied the UDRP rules in examining the WIPO panel's decision, while US courts often apply domestic patent law and ACPA(Anticyber -squatting Consumer's Protection Act) to review domain name arbitration decision rather than UDRP rules. In conclusion this article suggests that courts should restrict their judicial review on domain name administrative panel's decision at best. This will lead to facilitating the use of ADR in domain name dispute resolution and reducing the burden of courts' dockets.

• Journal of the Korean Mathematical Society
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• v.54 no.6
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• pp.1733-1757
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• 2017

Let $R={\oplus}_{{\alpha}{\in}{\Gamma}}R_{\alpha}$ be an integral domain graded by an arbitrary torsionless grading monoid ${\Gamma}$, ${\bar{R}}$ be the integral closure of R, H be the set of nonzero homogeneous elements of R, C(f) be the fractional ideal of R generated by the homogeneous components of $f{\in}R_H$, and $N(H)=\{f{\in}R{\mid}C(f)_v=R\}$. Let $R_H$ be a UFD. We say that a nonzero prime ideal Q of R is an upper to zero in R if $Q=fR_H{\cap}R$ for some $f{\in}R$ and that R is a graded UMT-domain if each upper to zero in R is a maximal t-ideal. In this paper, we study several ring-theoretic properties of graded UMT-domains. Among other things, we prove that if R has a unit of nonzero degree, then R is a graded UMT-domain if and only if every prime ideal of $R_{N(H)}$ is extended from a homogeneous ideal of R, if and only if ${\bar{R}}_{H{\backslash}Q}$ is a graded-$Pr{\ddot{u}}fer$ domain for all homogeneous maximal t-ideals Q of R, if and only if ${\bar{R}}_{N(H)}$ is a $Pr{\ddot{u}}fer$ domain, if and only if R is a UMT-domain.

• Journal of Navigation and Port Research
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• v.45 no.4
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• pp.204-211
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• 2021

The Maritime Domain Awareness system is a component that is necessary to enhance the awareness of all situations occurring at sea in relation to security, safety, economy, and the environment. To establish a marine situation recognition system that is currently being introduced in major maritime countries after approval by the International Maritime Organization's Maritime Safety Committee in 2010, operational measures should be established. For the purpose of establishing a technological foundation for the efficient construction of a Korean maritime situation recognition system, this study analyzed the status of each system (RADAR, VHF, and V-PASS, etc.) and proposed the application of data and communication standards.

• Korean Journal of Mathematics
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• v.19 no.3
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• pp.255-261
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• 2011

Let D be an integral domain, S be a torsion-free grading monoid such that the quotient group of S is of type (0, 0, 0, ${\ldots}$), and D[S] be the semigroup ring of S over D. We show that D[S] is an H-domain if and only if D is an H-domain and each maximal t-ideal of S is a $v$-ideal. We also show that if $\mathbb{R}$ is the eld of real numbers and if ${\Gamma}$ is the additive group of rational numbers, then $\mathbb{R}[{\Gamma}]$ is not an H-domain.

• Journal of the Korean Mathematical Society
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• v.48 no.1
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• pp.191-205
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• 2011

It is well known that for a Krull domain R, the divisor class group of R is a torsion group if and only if every subintersection of R is a ring of quotients. Thus a natural question is that under what conditions, for a non-Krull domain R, every (t-)subintersection (resp., t-linked overring) of R is a ring of quotients or every (t-)subintersection (resp., t-linked overring) of R is at. To address this question, we introduce the notions of *-compact packedness and *-coprime packedness of (an ideal of) an integral domain R for a star operation * of finite character, mainly t or w. We also investigate the t-theoretic analogues of related results in the literature.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.26 no.7
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• pp.1262-1269
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• 2002

It is inevitable to evaluate the life of turbine rotor because the operating periods of power plants need to be extended. For the test, seven kinds of specimens with different degradation levels were prepared by the isothermal heat treatment at $630^{\circ}C$. Magnetic methods utilizing Barkhausen noise coercive force($BN_c$) were applied to detect the degradation caused by thermal aging. Magnetic property of material is related with domain dynamics and that is affected by the microstructure of material. Therefore $BN_c$ is very sensitive to the microstructure change of the material. With the increase of degradation, $BN_c$ was decreased and this phenomenon is considered due to precipitations and grain size. The result was compared with Vickers hardness($H_v$) and coercive force($H_c$) to detect the relative variation, and was related with $H_v$ and YS to estimate the change of the mechanical properties with the degradation.

• Proceedings of the KSME Conference
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• 2001.06a
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• pp.250-255
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• 2001

It is inevitable to evaluate the life of turbine rotor because the operating periods of power plants need to be extended. The magnetic methods utilizing Magnetic Barkhausen noise curve were applied to detect the degradation caused by thermal aging. The Magnetic property of material depends on the domain dynamics and it is affected by the microstructure of material. Therefore the magnetic property is very sensitive to the microstructure change of the material. It is, thus, very useful to detect the state of degradation of varying materials. The test specimen made of 1Cr-1Mo-0.25V steel was used widely for turbine rotor material, and seven kinds of specimens with different degradation levels were prepared by the isothermal heat treatment at $630^{\circ}C$. With the increase of degradation, BHN was decreased. The result was compared with coercive force and vickers hardness.

• Journal of fish pathology
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• v.35 no.1
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• pp.121-128
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• 2022

Recent studies revealed that histone proteins are involved in innate immune responses during pathogen invasion as well as DNA packing. This study characterized the histone genes (H2A.V) of sevenband groupers and analyzed gene expression in NNV-infected sevenband groupers. The open reading frame (ORF) of H2A.V is 387 bp which encoded 128 amino acid residues. The deduced amino acid sequence of H2A.V harbor a highly conserved domain for H2A/H2B/H3 and H2A_C binding domain. Quantitative real-time PCR analysis showed that H2A.V had a high gene expression level in the brain and blood after being NNV-infected. An increase in extracellular histone protein in the blood has been identified as a biomarker for vascular function in humans. More research is required to understand histone's immune response at the protein level or in aquatic animals.