Browse > Article
http://dx.doi.org/10.7847/jfp.2022.35.1.121

Characterization of histone gene expression in sevenband grouper, Hyporthodus septemfasciatus against nervous necrosis virus infection  

Lee, Dong-Ryun (School of Marine and Fisheries Life Science, Pukyong National University)
Lee, A-Reum (School of Marine and Fisheries Life Science, Pukyong National University)
Krishnan, Rahul (Department of Aqualife Medicine, Chonnam National University)
Jang, Yo-Seb (Department of Aqualife Medicine, Chonnam National University)
Oh, Myung-Joo (Department of Aqualife Medicine, Chonnam National University)
Kim, Jong-Oh (School of Marine and Fisheries Life Science, Pukyong National University)
Publication Information
Journal of fish pathology / v.35, no.1, 2022 , pp. 121-128 More about this Journal
Abstract
Recent studies revealed that histone proteins are involved in innate immune responses during pathogen invasion as well as DNA packing. This study characterized the histone genes (H2A.V) of sevenband groupers and analyzed gene expression in NNV-infected sevenband groupers. The open reading frame (ORF) of H2A.V is 387 bp which encoded 128 amino acid residues. The deduced amino acid sequence of H2A.V harbor a highly conserved domain for H2A/H2B/H3 and H2A_C binding domain. Quantitative real-time PCR analysis showed that H2A.V had a high gene expression level in the brain and blood after being NNV-infected. An increase in extracellular histone protein in the blood has been identified as a biomarker for vascular function in humans. More research is required to understand histone's immune response at the protein level or in aquatic animals.
Keywords
Histone; nervous necrosis virus NNV; gene expression;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Grotmol S., Totland G.K., Kryvi H.: Detection of a nodavirus-like agent in heart tissue from reared Atlantic salmon Salmo salar suffering from cardiac myopathy syndrome (CMS). Diseases of Aquatic Organisms, 29: 79-84, 1997.   DOI
2 Hoeksema M., Tripathi S., White M., Qi L., Taubenberger J., van Eijk M., Haagsman H., Hartshorn K.L.: Arginine-rich histones have strong antiviral activity for influenza A viruses. Innate immunity, 21: 736-745, 2015.   DOI
3 Hoeksema M., van Eijk M., Haagsman H.P., Hartshorn K.L.: Histones as mediators of host defense, inflammation and thrombosis. Future Microbiology, 11: 441-453, 2016.   DOI
4 Iwamoto T., Nakai T., Mori K., Arimoto M., Furusawa I.: Cloning of the fish cell line SSN-1 for piscine nodaviruses. Diseases of Aquatic Organisms, 43: 81-89, 2000.   DOI
5 Kim J.O., Kim J.O., Kim W.S., Oh M.J.: Development and application of quantitative detection method for nervous necrosis virus (NNV) isolated from sevenband grouper Hyporthodus septemfasciatus. Asian Journal of Tropical Medicine, 9: 742-748, 2016.   DOI
6 Kim J.O., Kim J.O., Kim W.S., Oh M.J.: Characterization of the transcriptome and gene expression of brain tissue in sevenband grouper (Hyporthodus septemfasciatus) in response to NNV infection. Genes, 8: 31. 2017.   DOI
7 Krishnan R., Qadiri S.S.N., Kim J.O., Kim J.O., Oh M. J.: Validation of housekeeping genes as candidate internal references for quantitative expression studies in healthy and nervous necrosis virus-infected sevenband grouper (Hyporthodus septemfasciatus). Fisheries and Aquatic Sciences, 22: 1-8, 2019.   DOI
8 Lim C.: Results of the 2020 fish farming trend survey (In Korean). Statistics Korea, 2021.
9 Luger K., Mader A.W., Richmond R.K., Sargent D.F., Richmond T.J.: Crystal structure of the nucleosome core particle at 2.8 A resolution. Nature, 389: 251-260, 1997.   DOI
10 Nam B.H., Seo J.G., Go H.J., Lee M.J., Kim Y.O., Kim D.G., Lee S.J., Park N.G.: Purification and characterization of an antimicrobial histone H1-like protein and its gene from the testes of olive flounder, Paralichthys olivaceus. Fish & Shellfish Immunology, 33: 92-98, 2012.   DOI
11 Mezeth K.B., Patel S., Henriksen H., Szilvay A.M., Nerland A.H.: B2 protein from betanodavirus is expressed in recently infected but not in chronically infected fish. Diseases of Aquatic Organisms, 83: 97-103, 2009.   DOI
12 Tsourouktsoglou T., Warnatsch A., Ioannou M., Hoving D., Wang Q.: Papayannopoulos V. Histones, DNA, and citrullination promote neutrophil extracellular trap inflammation by regulating the localization and activation of TLR4. Cell Reports, 31: 107602, 2020.   DOI
13 Nishizawa T., Mori K., Furuhashi M., Nakai T., Furusawa I., Muroga K.: Comparison of the coat protein genes of five fish nodaviruses, the causative agents of viral nervous necrosis in marine fish. Journal of General Virology, 76: 1563-1569, 1995.   DOI
14 Placek B.J., Huang J., Kent J.R., Dorsey J., Rice L., Fraser N.W., Berger S.L.: The histone variant H3.3 regulates gene expression during lytic infection with herpes simplex virus type 1. Journal of Virology, 83: 1416-1421, 2009.   DOI
15 Schaefer U., Ho J.S.Y., Prinjha R.K., Tarakhovsky A.: The "histone mimicry" by pathogens. Cold Spring Harbor Symposia on quantitative biology, 78: 81-90, 2013.   DOI
16 Tamura, K., Stecher, G. and Kumar, S.: MEGA11: molecular evolutionary genetics analysis version 11. Molecular Biology, 38: 3022-3027, 2021.   DOI
17 Valero Y., Arizcun M., Esteban M.A., Cuesta A., Chaves-Pozo E.: Transcription of histones H1 and H2B is regulated by several immune stimuli in gilthead seabream and European sea bass. Fish & Shellfish Immunology, 57: 107-115, 2016.   DOI
18 Wang B., Gu H., Huang H., Wang H., Xia Z., Hu Y.: Characterization, expression, and antimicrobial activity of histones from japanese flounder Paralichthys olivaceus. Fish & Shellfish Immunology, 96: 235-244, 2020.   DOI
19 Tan C., Huang B., Chang S.F., Ngoh G.H., Munday B., Chen S.C., Kwang J.: Determination of the complete nucleotide sequences of RNA1 and RNA2 from greasy grouper (Epinephelus tauvina) nervous necrosis virus, Singapore strain. Journal of General Virology, 82: 647-653, 2001.   DOI
20 Villalba N., Baby S., Cha B.J., Yuan S.Y.: Site-specific opening of the blood-brain barrier by extracellular histones. Journal of Neuroinflammation, 17: 1-18, 2020.   DOI
21 MacMillan W.G., K.G. Hibbitt K.G.: The effect of antimicrobial proteins on the fine structure of Staphylococcus aureus. Journal of General Microbiology, 56: 373-377, 1969.   DOI
22 Over R.S. and Michaels S.D.: Open and closed: the roles of linker histones in plants and animals. Molecular Plant, 7: 481-491, 2014.   DOI
23 Kim C.S., Kim W.S., Nishizawa T, Oh M.J.: Prevalence of viral nervous necrosis (VNN) in sevenband grouper Epinephelus septemfasciatus farms. Journal of Fish Pathology, 25: 111-116, 2012.   DOI
24 Kong X, Wu X, Pei C, Zhang J, Zhao X, Li L, Nie G, Li X.: H2A and Ca-L-hipposin gene: Characteristic analysis and expression responses to Aeromonas hydrophila infection in Carassius aurutus. Fish & Shellfish Immunology, 63: 344-352, 2017.   DOI
25 Lin C.H., Chen J.J., Cheng C.M.: Developing a virus-binding bacterium expressing Mx protein on the bacterial surface to prevent grouper nervous necrosis virus infection. Journal of Microbiology and Biotechnology. 31: 1088-1097, 2021.   DOI