Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Characterization of histone gene expression in sevenband grouper, Hyporthodus septemfasciatus against nervous necrosis virus infection

  • Lee, Dong-Ryun (School of Marine and Fisheries Life Science, Pukyong National University) ;
  • Lee, A-Reum (School of Marine and Fisheries Life Science, Pukyong National University) ;
  • Krishnan, Rahul (Department of Aqualife Medicine, Chonnam National University) ;
  • Jang, Yo-Seb (Department of Aqualife Medicine, Chonnam National University) ;
  • Oh, Myung-Joo (Department of Aqualife Medicine, Chonnam National University) ;
  • Kim, Jong-Oh (School of Marine and Fisheries Life Science, Pukyong National University)
  • Received : 2022.06.01
  • Accepted : 2022.06.10
  • Published : 2022.06.30
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Abstract

Recent studies revealed that histone proteins are involved in innate immune responses during pathogen invasion as well as DNA packing. This study characterized the histone genes (H2A.V) of sevenband groupers and analyzed gene expression in NNV-infected sevenband groupers. The open reading frame (ORF) of H2A.V is 387 bp which encoded 128 amino acid residues. The deduced amino acid sequence of H2A.V harbor a highly conserved domain for H2A/H2B/H3 and H2A_C binding domain. Quantitative real-time PCR analysis showed that H2A.V had a high gene expression level in the brain and blood after being NNV-infected. An increase in extracellular histone protein in the blood has been identified as a biomarker for vascular function in humans. More research is required to understand histone's immune response at the protein level or in aquatic animals.

Keywords

Acknowledgement

This work was supported by a Research Grant of Pukyong National University (2021).

References

  1. Grotmol S., Totland G.K., Kryvi H.: Detection of a nodavirus-like agent in heart tissue from reared Atlantic salmon Salmo salar suffering from cardiac myopathy syndrome (CMS). Diseases of Aquatic Organisms, 29: 79-84, 1997. https://doi.org/10.3354/dao029079
  2. Hoeksema M., Tripathi S., White M., Qi L., Taubenberger J., van Eijk M., Haagsman H., Hartshorn K.L.: Arginine-rich histones have strong antiviral activity for influenza A viruses. Innate immunity, 21: 736-745, 2015. https://doi.org/10.1177/1753425915593794
  3. Hoeksema M., van Eijk M., Haagsman H.P., Hartshorn K.L.: Histones as mediators of host defense, inflammation and thrombosis. Future Microbiology, 11: 441-453, 2016. https://doi.org/10.2217/fmb.15.151
  4. Iwamoto T., Nakai T., Mori K., Arimoto M., Furusawa I.: Cloning of the fish cell line SSN-1 for piscine nodaviruses. Diseases of Aquatic Organisms, 43: 81-89, 2000. https://doi.org/10.3354/dao043081
  5. Kim C.S., Kim W.S., Nishizawa T, Oh M.J.: Prevalence of viral nervous necrosis (VNN) in sevenband grouper Epinephelus septemfasciatus farms. Journal of Fish Pathology, 25: 111-116, 2012. https://doi.org/10.7847/JFP.2012.25.2.111
  6. Kim J.O., Kim J.O., Kim W.S., Oh M.J.: Development and application of quantitative detection method for nervous necrosis virus (NNV) isolated from sevenband grouper Hyporthodus septemfasciatus. Asian Journal of Tropical Medicine, 9: 742-748, 2016. https://doi.org/10.1016/j.apjtm.2016.06.014
  7. Kim J.O., Kim J.O., Kim W.S., Oh M.J.: Characterization of the transcriptome and gene expression of brain tissue in sevenband grouper (Hyporthodus septemfasciatus) in response to NNV infection. Genes, 8: 31. 2017. https://doi.org/10.3390/genes8010031
  8. Kong X, Wu X, Pei C, Zhang J, Zhao X, Li L, Nie G, Li X.: H2A and Ca-L-hipposin gene: Characteristic analysis and expression responses to Aeromonas hydrophila infection in Carassius aurutus. Fish & Shellfish Immunology, 63: 344-352, 2017. https://doi.org/10.1016/j.fsi.2017.02.028
  9. Krishnan R., Qadiri S.S.N., Kim J.O., Kim J.O., Oh M. J.: Validation of housekeeping genes as candidate internal references for quantitative expression studies in healthy and nervous necrosis virus-infected sevenband grouper (Hyporthodus septemfasciatus). Fisheries and Aquatic Sciences, 22: 1-8, 2019. https://doi.org/10.1186/s41240-019-0117-4
  10. Lim C.: Results of the 2020 fish farming trend survey (In Korean). Statistics Korea, 2021.
  11. Lin C.H., Chen J.J., Cheng C.M.: Developing a virus-binding bacterium expressing Mx protein on the bacterial surface to prevent grouper nervous necrosis virus infection. Journal of Microbiology and Biotechnology. 31: 1088-1097, 2021. https://doi.org/10.4014/jmb.2103.03036
  12. Luger K., Mader A.W., Richmond R.K., Sargent D.F., Richmond T.J.: Crystal structure of the nucleosome core particle at 2.8 A resolution. Nature, 389: 251-260, 1997. https://doi.org/10.1038/38444
  13. Mezeth K.B., Patel S., Henriksen H., Szilvay A.M., Nerland A.H.: B2 protein from betanodavirus is expressed in recently infected but not in chronically infected fish. Diseases of Aquatic Organisms, 83: 97-103, 2009. https://doi.org/10.3354/dao02015
  14. MacMillan W.G., K.G. Hibbitt K.G.: The effect of antimicrobial proteins on the fine structure of Staphylococcus aureus. Journal of General Microbiology, 56: 373-377, 1969. https://doi.org/10.1099/00221287-56-3-373
  15. Nam B.H., Seo J.G., Go H.J., Lee M.J., Kim Y.O., Kim D.G., Lee S.J., Park N.G.: Purification and characterization of an antimicrobial histone H1-like protein and its gene from the testes of olive flounder, Paralichthys olivaceus. Fish & Shellfish Immunology, 33: 92-98, 2012. https://doi.org/10.1016/j.fsi.2012.04.006
  16. Nishizawa T., Mori K., Furuhashi M., Nakai T., Furusawa I., Muroga K.: Comparison of the coat protein genes of five fish nodaviruses, the causative agents of viral nervous necrosis in marine fish. Journal of General Virology, 76: 1563-1569, 1995. https://doi.org/10.1099/0022-1317-76-7-1563
  17. Over R.S. and Michaels S.D.: Open and closed: the roles of linker histones in plants and animals. Molecular Plant, 7: 481-491, 2014. https://doi.org/10.1093/mp/sst164
  18. Placek B.J., Huang J., Kent J.R., Dorsey J., Rice L., Fraser N.W., Berger S.L.: The histone variant H3.3 regulates gene expression during lytic infection with herpes simplex virus type 1. Journal of Virology, 83: 1416-1421, 2009. https://doi.org/10.1128/JVI.01276-08
  19. Schaefer U., Ho J.S.Y., Prinjha R.K., Tarakhovsky A.: The "histone mimicry" by pathogens. Cold Spring Harbor Symposia on quantitative biology, 78: 81-90, 2013. https://doi.org/10.1101/sqb.2013.78.020339
  20. Tamura, K., Stecher, G. and Kumar, S.: MEGA11: molecular evolutionary genetics analysis version 11. Molecular Biology, 38: 3022-3027, 2021. https://doi.org/10.1093/molbev/msab120
  21. Tan C., Huang B., Chang S.F., Ngoh G.H., Munday B., Chen S.C., Kwang J.: Determination of the complete nucleotide sequences of RNA1 and RNA2 from greasy grouper (Epinephelus tauvina) nervous necrosis virus, Singapore strain. Journal of General Virology, 82: 647-653, 2001. https://doi.org/10.1099/0022-1317-82-3-647
  22. Tsourouktsoglou T., Warnatsch A., Ioannou M., Hoving D., Wang Q.: Papayannopoulos V. Histones, DNA, and citrullination promote neutrophil extracellular trap inflammation by regulating the localization and activation of TLR4. Cell Reports, 31: 107602, 2020. https://doi.org/10.1016/j.celrep.2020.107602
  23. Valero Y., Arizcun M., Esteban M.A., Cuesta A., Chaves-Pozo E.: Transcription of histones H1 and H2B is regulated by several immune stimuli in gilthead seabream and European sea bass. Fish & Shellfish Immunology, 57: 107-115, 2016. https://doi.org/10.1016/j.fsi.2016.08.019
  24. Villalba N., Baby S., Cha B.J., Yuan S.Y.: Site-specific opening of the blood-brain barrier by extracellular histones. Journal of Neuroinflammation, 17: 1-18, 2020. https://doi.org/10.1186/s12974-019-1655-5
  25. Wang B., Gu H., Huang H., Wang H., Xia Z., Hu Y.: Characterization, expression, and antimicrobial activity of histones from japanese flounder Paralichthys olivaceus. Fish & Shellfish Immunology, 96: 235-244, 2020. https://doi.org/10.1016/j.fsi.2019.11.065