• Publications of The Korean Astronomical Society
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• v.30 no.2
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• pp.265-266
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• 2015

A search for luminous white dwarfs (WDs) in several nearby Galactic globular clusters (GCs) was carried out using the deep and homogeneous photometric catalog of Galactic GCs taken with the ACS/WFC aboard the Hubble Space Telescope (HST) by Sarajedini et al. and Anderson et al- It resulted in the identification of luminous WD candidates in the GCs M13 (NGC 6205) and M22 (NGC 6656). The purpose of the present study is to identify luminous WDs in the deep and homogeneous V versus V - I color-magnitude diagrams (CMDs) of several nearby Galactic GCs taken with the ACS/WFC aboard the HST. Using photometric data for the GCs M13 and M22 that are now in the public domain, the V versus V - I CMDs of the GCs M13 and M22 were constructed. Many spurious detections in the CMDs were removed using the photometric quality parameters qfit(V) and qfit(I), and a radial restriction was applied to the CMDs to remove the central stars with higher photometric errors due to central crowding. From each resultant V versus V - I CMD of the GCs M13 and M22, a dozen or so luminous WD candidates were identified. They were confirmed as stellar objects in the accompanying ACS/WFC images and their positions in the CMDs were in the bright part of the DA WD cooling curve. Therefore, the luminous WD candidates in the GCs M13 and M22 seem to be true luminous WDs, and spectroscopic observations are needed to confirm their true identity.

• IMMUNE NETWORK
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• v.21 no.6
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• pp.39.1-39.18
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• 2021

The high virulent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus that emerged in China at the end of 2019 has generated novel coronavirus disease, coronavirus disease 2019 (COVID-19), causing a pandemic worldwide. Every country has made great efforts to struggle against SARS-CoV-2 infection, including massive vaccination, immunological patients' surveillance, and the utilization of convalescence plasma for COVID-19 therapy. These efforts are associated with the attempts to increase the titers of SARS-CoV-2 neutralizing Abs (nAbs) generated either after infection or vaccination that represent the body's immune status. As there is no standard therapy for COVID-19 yet, virus eradication will mainly depend on these nAbs contents in the body. Therefore, serological nAbs neutralization assays become a requirement for researchers and clinicians to measure nAbs titers. Different platforms have been developed to evaluate nAbs titers utilizing various epitopes sources, including neutralization assays based on the live virus, pseudovirus, and neutralization assays utilizing recombinant SARS-CoV-2 S glycoprotein receptor binding site, receptor-binding domain. As a standard neutralization assay, the plaque reduction neutralization test (PRNT) requires isolation and propagation of live pathogenic SARS-CoV-2 virus conducted in a BSL-3 containment. Hence, other surrogate neutralization assays relevant to the PRNT play important alternatives that offer better safety besides facilitating high throughput analyses. This review discusses the current neutralization assay platforms used to evaluate nAbs, their techniques, advantages, and limitations.

• Journal of Life Science
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• v.21 no.7
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• pp.1032-1038
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• 2011

Recently identified Ciona intestinalis voltage sensor-containing phosphatase (Ci-VSP) consists of an ion channel-like transmembrane domain (VSD) and a phosphatase-like domain. Ci-VSP senses the change of membrane potential by its VSD and works as a phosphoinositide phosphatase by its phosphatase domain. In this study, we present the construction of His-tagged phosphatase-like domain of Ci-VSP, its recombinant expression and purification, and its enzymatic activity behavior in order to examine the biochemical behavior of phosphatase domain of Ci-VSP without interference. We found that Ci-VSP(248-576)-His can be eluted with an elution buffer containing 25 mM NaCl and 100 mM imidazole during His-tag purification. In addition, we found the proper measurement condition for kinetics study of Ci-VSP(248-576)-His against p-nitrophenyl phosphate (pNPP). We measured the kinetic constant of Ci-VSP(248-576)-His at $37^{\circ}C$, pH 5.0 or 5.5, under 30 min of reaction time, and less than $2.0\;{\mu}g$ of protein amount. With these conditions, we acquired that Ci-VSP(248-576)-His has $K_m$ of $354{\pm}0.143\;{\mu}M$, $V_{max}$ of $0.0607{\pm}0.0137\;{\mu}mol$/min/mg and $k_{cat}$ of $0.359{\pm}0.009751\;min^{-1}$ for pNPP dephosphorylation. Therefore, we produced a pure form of Ci-VSP(248-576)-His, and this showed a higher activity against pNPP. This purified protein will provide the road to a structural investigation on an interesting protein, Ci-VSP.

• Journal of the Korean Institute of Telematics and Electronics C
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• v.35C no.6
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• pp.39-47
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• 1998

A fourth-order $\Sigma$-$\Delta$ modulator is designed and implemented in 0.6 $\mu\textrm{m}$ CMOS technology. The modulator is verified by introducing nonlinear factors such as DC gain and slew rate in system model that determines the transfer function in S-domain and in time-domain. Dynamic range is more than 110 dB and the peak SM is 102.6 dB at a clock rate of 2.8224 MHz for voiceband signal. The structure of a ∑-$\Delta$ modulator is a modified fourth-order ∑-$\Delta$ modulator using direct feedback loop method, which improves performance and consumes less power. The transmission zero for noise is located in the first-second integrator loop, which reduces entire size of capacitors, reduces the active area of the chip, improves the performance, and reduces power dissipation. The system is stable because the output variation with respect to unit time is small compared with that of the third integrator. It is easy to implement because the size of the capacitor in the first integrator, and the size of the third integrator is small because we use the noise reduction technique. This paper represents a new design method by modeling that conceptually decides transfer function in S-domain and in Z-domain, determines the cutoff frequency of signal, maximizes signal power in each integrator, and decides optimal transmission-zero frequency for noise. The active area of the prototype chip is 5.25$\textrm{mm}^2$, and it dissipates 10 mW of power from a 5V supply.

• Korean Journal of Poultry Science
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• v.45 no.3
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• pp.209-217
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• 2018

The innate immune recognition is based on the detection of microbial products. Toll-like receptors (TLRs) located on the cell surface and the endosome senses microbial components and nucleic acids, respectively. Chicken TLRs mediate immune responses by sensing ligands from pathogens, have been studied as immune adjuvants to increase the efficacy of vaccines. Single nucleotide polymorphisms (SNPs) of TLR3 and TLR4 genes in chicken were associated with resistance and susceptibility to viral infection. In this study, SNPs of chTLR3 and chTLR4 genes were retrieved from public database and annotated with chicken reference genome. Three-dimensional models of the chTLR3 and chTLR4 proteins were built using a Swiss modeler. We identified 35 and 13 nsSNPs in chTLR3 and chTLR4 genes respectively. Sorting Intolerant from Tolerant (SIFT) and Polymorphism Phenotyping v2 (Polyphen-2) analyses, suggested that, out of 35 and 13 nsSNPs, 4 and 2 SNPs were identified to be deleterious in chTLR3 and chTLR4 gene respectively. In chTLR3, 1 deleterious SNP was located in ectodomain and 3 were located in the Toll / IL-1 receptor (TIR) domain. Further structural model of chTLR3-TIR domain suggested that 1 deleterious SNP be present in the B-B loop region, which is important for TIR-TIR domain interactions in the downstream signaling. In chTLR4, the deleterious SNPs were located both in the ectodomain and TIR domain. SNPs predicted for chTLR3 and chTLR4 in this study, might be related to resistance or susceptible to viral infection in chickens. Results from this study will be useful to develop the effective measures in chicken against infectious diseases.

• Molecules and Cells
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• v.44 no.2
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• pp.88-100
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• 2021

Anoctamin 6/TMEM16F (ANO6) is a dual-function protein with Ca2+-activated ion channel and Ca2+-activated phospholipid scramblase activities, requiring a high intracellular Ca2+ concentration (e.g., half-maximal effective Ca2+ concentration [EC50] of [Ca2+]i > 10 μM), and strong and sustained depolarization above 0 mV. Structural comparison with Anoctamin 1/TMEM16A (ANO1), a canonical Ca2+-activated chloride channel exhibiting higher Ca2+ sensitivity (EC50 of 1 μM) than ANO6, suggested that a homologous Ca2+-transferring site in the N-terminal domain (Nt) might be responsible for the differential Ca2+ sensitivity and kinetics of activation between ANO6 and ANO1. To elucidate the role of the putative Ca2+-transferring reservoir in the Nt (Nt-CaRes), we constructed an ANO6-1-6 chimera in which Nt-CaRes was replaced with the corresponding domain of ANO1. ANO6-1-6 showed higher sensitivity to Ca2+ than ANO6. However, neither the speed of activation nor the voltage-dependence differed between ANO6 and ANO6-1-6. Molecular dynamics simulation revealed a reduced Ca2+ interaction with Nt-CaRes in ANO6 than ANO6-1-6. Moreover, mutations on potentially Ca2+-interacting acidic amino acids in ANO6 Nt-CaRes resulted in reduced Ca2+ sensitivity, implying direct interactions of Ca2+ with these residues. Based on these results, we cautiously suggest that the net charge of Nt-CaRes is responsible for the difference in Ca2+ sensitivity between ANO1 and ANO6.

• Proceeding of EDISON Challenge
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• 2015.03a
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• pp.312-319
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• 2015

본 연구에서는 전자구조 계산을 통해 태양전지의 소재로 적합한 Graphene과 h-BN의 합성물을 찾아내었다. 태양전지가 최대 효율을 가지려면 소재의 band gap이 약 1.2 eV이어야 하는데, 본 연구에서는 이러한 물질을 다수 찾아 내었고 이런 물질들의 특징을 분석하였다. carbon domain이 넓을수록 band gap이 작았고 carbon ring이 유지될수록 cohesive energy가 컸다. 이를 다시 적용하여 적합한 구조를 예상하였으며 이와 잘 맞는 계산결과를 얻었다.

• Proceedings of the Optical Society of Korea Conference
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• 2008.02a
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• pp.305-306
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• 2008

We have developed several types of optical sources for OCT applications. A high nonlinear PCF with end sealing and a short pulse laser were used to improve of OCT resolution. Additionally, an wavelength sept fiber laser was also developed for frequency-domain OCT.

• Interaction and multiscale mechanics
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• v.3 no.1
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• pp.81-94
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• 2010

The steady state response of a rotating generalized thermoelastic solid to a moving point load has been investigated. The transformed components of displacement, force stress and temperature distribution are obtained by using Fourier transformation. These components are then inverted and the results are obtained in the physical domain by applying a numerical inversion method. The numerical results are presented graphically for a particular model. A particular result is also deduced from the present investigation.

• Journal of the Chungcheong Mathematical Society
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• v.20 no.1
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• pp.65-70
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• 2007

We study the existence of nontrivial solutions of the Gradient type Dirichlet boundary value problem for elliptic systems of the form $-{\Delta}U(x)={\nabla}F(x,U(x)),x{\in}{\Omega}$, where ${\Omega}{\subset}R^N(N{\geq}1)$ is a bounded regular domain and U = (u, v) : ${\Omega}{\rightarrow}R^2$. To study the system we use the liking theorem on product space.