• Journal of Microbiology and Biotechnology
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• v.11 no.6
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• pp.1055-1060
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• 2001

Experimental data for the on-line estimation of cell concentration and growth rate are presented. For this purpose, we utilized the on-line calculation of the oxygen uptake rate (OUR), which was derived from a liquid phase dynamic mass balance for the oxygen during the active growth phase in cyclosporin A (CyA) fermentation. The cell yield coefficient, based on the oxygen $(Y_{x/o})$for both suspended and immobilized cells of Tolypocladium inflatum, was estimated as $1.9 gDCW/gO_2$ from a very good linear correlation between the cell mass produced and the total oxygen consumed. The calculated yield showed a good agreement with the value of $(Y_{x/o})$ generated from the correlation between the cell growth rate and the oxygen uptake rate. In addition, further experimental data are given, which were also applied to determine the specific oxygen uptake rate of T. inflatum cells during the exponential phase of CyA fermentation. A theoretical basis for the analysis of these fermentation parameters is also provided.

• KSBB Journal
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• v.13 no.5
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• pp.606-614
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• 1998

In this study, biodegradation rate of Arabian light crude oil by mixed cultures of selected petroleum-degraders was determined. Their modes of hydrocarbon uptake were then observed to determine whether there are differences in biodegradation rate by the mixed cultures. By the mixed cultures of petroleum-degraders having same modes of hydrocarbon uptake, such as strain US1 and K1 (using pseudo-solubilized hydrocarbons by a biosurfactants), K2-2 and P1(using hydrocarbons by direct contact), CL 180 and IC-10 (mixed type of uptake modes), the biodegradation rates of aliphatics in the crude oil were increased more than those by their pure cultures, about 40%, 25% and 20%, respectively. Biodegradation rate of strain KH3-2 (using only water- dissolved hydrocarbons) was increased by mixed cultures with strain K1, CL180 or IC-10 possessing high emulsifying activity. However, the biodegradation rate of the crude oil was decreased about 20%-40% by the mixed cultures of petroleum-degraders having different mode of hydrocarbon uptake, such as addition of strain US1 or K1 in the cultures of K2-2 or P1. Biosurfactants produced by US1 or K1 seems to enhance the emulsification of crude oil in aqueous phase but inhibit the attachment of K2-2 or P1 to crude oil. As same phenomena, the addition to Triton X-100 into the culture of strain US1, K1, CL180, IC-10 or KH3-2 increased the biodegradation rate, but the addition in the culture of strain K2-2 or P1 decreased the biodegradation rate. The mixed culture made of CL180, IC-10 and KH3-2 degraded 61.5% of aliphatics and 69% of aromatics in 3% (v/v) of Arabian light crude oil added.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.41 no.4
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• pp.301-304
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• 2008

For bioremediation of the benthic layer uptake kinetics of phosphate by microphytobenthos Nitzschia sp.(JFH200406) were investigated. A short-term phosphate uptake revealed that the maximum uptake rate(${\rho}_{max}$) and half-saturation constant($K_s$) were 0.132 pmol/cell/hr and 502.6 ${\mu}M$, respectively. The maximum specific uptake rate calculated between ${\rho}_{max}$ and the phosphorus cell quota($Q_p$), calculated from Strathmann equation, was 14.4/day. The values of these parameters indicate that Nitzschia sp. accommodates well to surroundings of high phosphate, and can uptake over 14-times more than the phosphorus cell quota. Thus, microphytobenthos Nitzschia sp. may be a useful species for bioremediation of the benthic layer.

• Analytical Science and Technology
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• v.11 no.2
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• pp.130-134
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• 1998

Polyamines were analyzed in young cotyledons of spring radish. The uptake rate of SPD were transported within 20 min lineary and reached the saturation phase after 1hr. The uptake rate of SPD decreased gradually with the time by the increasing amount of SPD inside the cells. The uptake of PA depends on the external pH. The optimal pH of PA uptake are pH 7.5. $K_m-$ and $V_{max}-$values depend on external pH also. The uptake rate of PA was inhibited by external KCI, which depolarized membrane potential in the cells.

• Journal of the Korean Institute of Landscape Architecture
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• v.26 no.2
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• pp.38-53
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• 1998

This study quantified annual net carbon uptake by urban landscape trees and provided equations to estimate it for Ginkgo biloba, platanus occidentalis, Zelkova serrata and Acer palmatum, based on measurement of exchange rate for two years growing seasons from Sep., 1995 to Aug., 1997. The carbon uptake was significantly influenced by photosynthetic capacity, photon flux density and pruning. Ginkgo biloba showed the highest rate of net CO\sub 2\ uptake per unit leaf area and Acer palmatum did the lowest rate among those species. A tree shaded by adjacent building over the growing seasons showed net CO\sub2\ uptake per unit leaf area much lower than another tree of the same species less shaded. Annual net carbon uptake per tree was 19kg for Zelkova serrata, but only 1 kg for Ginkgo biloba and Platanus occidentalis with crown volume dwarfed from pruning. One Zekoval serrata tree annually offset carbon emission from consumption of about 32 liter of gasoline or 83 kWh of electricity. Strategies to improve CO\sub 2\ uptake by urban landscape trees include planting of species with high potosynthetic capacity, sunlight-guaranteed road and building layout for street trees, planting of shade-tolerant species in the north of buildings, and relocation of utility lines to underground and minimized pruning.

• Korean Journal of Veterinary Research
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• v.12 no.1
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• pp.21-24
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• 1972

In native and Saanen goats, the thyroidal iodine uptake rate and its correlation between thyroidal activity and body gain were studied. The results were as follows: 1. The thyroidal iodine-131 uptake rate in native and Saanen goats were 10.46% and 21.26%, respectively, and the difference was statistically significant (P<0.01). 2. The correlation coefficients between monthly body gain and thyroidal iodine-131 uptake rate were -0.32 and -0.46 in native and Saanen goats, respectively. The correlations were negative and not significant between the rates.

• Korean Journal of Plant Tissue Culture
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• v.27 no.1
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• pp.47-50
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• 2000

In suspension cultures of Streptanthus. the uptake rate of sugar was increased during the ceil starvation of sugar in the medium. The maximal uptake rate obtained with 3 days of cell starvation. Sugar transport system induced by the sugar starvation was completely inhibited by 10 $\mu$M cycloheximide. Plant cells are known to possess only one sugar transport system, but the uptake rate of glucose obtained a saturated kinetic while the one of sucrose had two different kinetics after the sugar starvation. Induced sugar transport systems had different kinetics compared to plant cell. These results showed that higher plants have adaptable ability to induce new sugar transport systems when the environment changed unsuitable.

• Journal of radiological science and technology
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• v.4 no.1
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• pp.55-62
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• 1981

This study was conducted, during the period of 20-30th, July in 1981, to survey measurement methods in thyroid uptake rate test in Seoul city. The results were summarized as follows: 1. For the great part of nuclear medcine department, a mount of radioiodine($^{131}I$) administrated to the patients was $50-100{\mu}Ci$ in thyroid uptake rate test. 2. Distribution of scintillation, counter with crystal size of $1\frac{1}{2}inch$ was 43%, 3inch(22%), 2.5inch(14%) and $2\frac{1}{2}inch$ was 7% in RAI uptake rate test. 3. When RAI uptake rate test was performed, distribution of collimator in use was flat field type collimator(78%) in general and cylindrical type collimator was 22%. 4. High voltage applied to the P-M tube was $900{\sim}1000V$(50%) and most units provided $3{\sim}15%$ of the window range for the $^{131}I$ peak $\gamma-ray$ energy. 5. Distribution on the use of neck phantom for measurements standard solution was 57% and distribution of b filter in use for room background counts and extrathyroidal tissue was 43% and 50%. 6. The distance between the counter and the source was 25cm(58%) in measuring radioactivity of standard solution, thyroid tissue and background radioactivity count. 7. The early uptake measurements(2, 4, 6 hours) are done after administration of the radioiodine dose and also 24-hour and 48-hour uptake measurements are done in routine test.

• Korean Journal of Veterinary Research
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• v.6 no.1
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• pp.24-30
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• 1966

In order to observe the effect of the injected radioactive iodine-$I^{131}$ on the uptake in thyroid of normal male guinea pigs and P.B.$I^{131}$ conversion ratio of $I^{131}$ in serum, 24 matured male guinea pigs were divided in 4 groups and $35{\mu}c$, $70{\mu}c$, $140{\mu}c$ and $280{\mu}c$ per kg of body weight respectively were injected subcutaneously. 1. The uptake rates of radioactveiodine-$I^{131}$ by external counts of thyroidal uptake reached the maximum level of uptake in 24 hours after injection. 2. As the injected amount increases, the uptake rates of maximum levels and release rate were increased. 3. Uptake rate in the removed thyroid have shown no statistical in the $35{\mu}c$ and $70{\mu}c$ groups of injected guinea pigs. 4. There was no statistical significance in $140{\mu}c$ and $280{\mu}c$ groups of injected guinea pigs. 5. P.B.$I^{131}$ conversion ratio of $I^{131}$ in serum was not in proportion to injected amounts: 61.0%(35), 70.2%(70), 75.3%(140) and 64.8%(280).

• The Korean Journal of Nuclear Medicine
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• v.33 no.1
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• pp.49-56
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• 1999

Purpose: Heart to liver ratio on T1-201 per rectal scintigraphy (shunt index) is known to be useful in the assessment of portal systemic shunt. We assessed T1-201 uptake pattern and early liver/heart uptake rate of T1-201 and correlated with shunt index in patients with chronic active hepatitis (CAH) and liver cirrhosis (LC). Materials and Methods: Fifty eight patients with biopsy-proven chronic liver disease (35 with CAH, 23 with LC) underwent T1-201 per rectum scintigraphy after instillation of 18.5 MBq of T1-201 into the upper rectum. We evaluated hepatic uptake (type 1 : homogeneous, 2: inhomogeneous segmental, 3: inhomogeneous nonsegmental) and extrahepatic uptake of spleen, heart and kidney (grade 0: no uptake, 1: less than liver, 2: equal to liver, 3: greater than liver). We measured the early liver/heart uptake rate (the slope of the liver to heart uptake ratio for 10 min) and shunt index (heart to liver uptake ratio). T1-201 uptake pattern and early liver/heart uptake rate of T1-201 was correlated with the pathologic diagnosis and shunt index. Results: Hepatic uptake patterns of type 1 and 2 were dominant in CAH (CAH: 27/35, LC. 8/23), and type 3 in LC (CAH: 8/35, LC: 15/23)(p<0.005). The grades of extrahepatic uptake were higher in LC than in CAH (spleen: p<0.001, other soft tissue: p<0.005). The early liver/heart uptake rate of CAH ($0.110{\pm}0.111$) was significantly higher than that of LC ($0.014{\pm}0.090$)(p<0.001). The sensitivity and specificity of the early liver/heart uptake rate were 77.7% and 67.7% in differentiating LC from CAH. There was negative correlation between early liver/heart uptake rate and shunt index (r=-0.3347, p<0.01). Conclusion: Hepatic and extrahepatic uptake pattern and early liver/heart uptake rate on T1-201 per rectum scintigraphy are useful in the assessment of portal systemic shunt in patients with chronic liver disease.