Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
권호 표지
DOI QR코드

DOI QR Code

Nutrient Uptake Kinetics of Nitzschia sp. for Bioremediation of the Benthic Layer

저질 환경 개선을 위한 Nitzschia sp.의 영양염 흡수 동력학

  • Oh, Seok-Jin (Korea Inter-University Institute of Ocean Science, Pukyong National University)
  • 오석진 (부경대학교 해양과학공동연구소)
  • Published : 2008.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

For bioremediation of the benthic layer uptake kinetics of phosphate by microphytobenthos Nitzschia sp.(JFH200406) were investigated. A short-term phosphate uptake revealed that the maximum uptake rate(${\rho}_{max}$) and half-saturation constant($K_s$) were 0.132 pmol/cell/hr and 502.6 ${\mu}M$, respectively. The maximum specific uptake rate calculated between ${\rho}_{max}$ and the phosphorus cell quota($Q_p$), calculated from Strathmann equation, was 14.4/day. The values of these parameters indicate that Nitzschia sp. accommodates well to surroundings of high phosphate, and can uptake over 14-times more than the phosphorus cell quota. Thus, microphytobenthos Nitzschia sp. may be a useful species for bioremediation of the benthic layer.

Keywords

References

  1. Abe, H. 1985. Ouyo Sugaku Nyumon. Baifu Kan, Tokyo, Japan, 1-215
  2. Cembella, A.D., N.J. Antia and P.J. Harrison. 1984. The utilization of inorganic and organic phosphorus compounds as nutrients by eukaryotic microalgae: A multidisciplinary perspective. CRC Critic. Rev. Microbiol., 11, 13-81 https://doi.org/10.3109/10408418409105902
  3. Dugdale, R.C. 1967. Nutrient limitation in the sea: dynamic, identification, and significance. Limnol. Oceanogr., 12, 685-695 https://doi.org/10.4319/lo.1967.12.4.0685
  4. Eppley, R.W., J.N. Rogers and J.J. McCarthy. 1969. Half-saturation constants for uptake of nitrate and ammonium by marine phytoplankton. Limnol. Oceanogr., 14, 912-920 https://doi.org/10.4319/lo.1969.14.6.0912
  5. Guillard, R.R.L. and P.E. Hargraves. 1993. Strichochrysis immobilis is a diatom, not a chrysophyte. Phycologia, 32, 234-236 https://doi.org/10.2216/i0031-8884-32-3-234.1
  6. Harrison, P.J., J.S. Parslow and H.L. Conway. 1989. Determination of nutrient uptake kinetic parameters: a comparison of methods. Mar. Ecol. Prog. Ser., 52, 301-312 https://doi.org/10.3354/meps052301
  7. Keller, M.D., R.C. Selvin, W. Claus and R.R.L. Guillard. 1987. Media for the culture of oceanic ultraphyto- plankton. J. Phycol., 23, 633-638 https://doi.org/10.1111/j.1529-8817.1987.tb04217.x
  8. Lee, C.B. and D.S. Kim. 1990. Pore water chemistry of intertidal mudflat sediment. 1. Seasonal variability of nutrient profiles (S, N, P). J. Oceanol. Soc. Kor., 25, 8-20
  9. Oh, S.J., T. Yamamoto and H.Y. Yoon. 2006. Uptake and excretion of dissolved organic phosphorus by two dinoflagellates, Alexandrium tamarense Lebour (Balech) and Gymnodinium catenatum Graham. J. Fish. Sci. Technol., 9, 30-37 https://doi.org/10.5657/fas.2006.9.1.030
  10. Oh, S.J., D.S. Park, H.S. Yang, Y.H. Yoon, T. Honjo. 2007. Bioremediation on the benthic layer in polluted inner bay by promotion of microphytobenthos growth using light emitting diode (LED). J. Kor. Soc. Mar. Environ. Eng., 10, 93-101
  11. Oh, S.J., D.I. Kim, T. Sajima, Y. Shimasaki, Y. Matsuyama, Y. Oshima, T. Honjo and H.S. Yang. 2008. Effects of irradiance of various wavelengths from light-emitting diodes on the growth of the harmful dinoflagellate Heterocapsa circularisquama and the diatom Skeletonema costatum. Fish. Sci., 74, 137-145 https://doi.org/10.1111/j.1444-2906.2007.01503.x
  12. Provasoli, L., K. Shiraishi and J.R. Lance. 1959. Nutritional idiosyncrasies of Artemia and Tigriopus in monoxenic culture. Ann. N.Y. Sci., 77, 250-261 https://doi.org/10.1111/j.1749-6632.1959.tb36905.x
  13. Redfield, A.C. 1934. On the proportions of organic derivatives in sea water and their relation to the com- position of plankton. In: James Johnstone Memorial Volume. Daniel R.J., ed. University Press of Liverpool, U.K., 177-192
  14. Strathmann, R.R. 1967. Estimating the organic carbon content of phytoplankton from cell volume or plasma volume, Limnol. Oceanogr., 12, 411-418 https://doi.org/10.4319/lo.1967.12.3.0411
  15. Strickland, J.D.H. and T.R. Parsons. 1972. A Practical Handbook of Seawater Analysis, 2nd ed. Bulletin of Fisheries Research Board of Canada, Canada, 1-310
  16. Suzuki, M. and T. Yamamoto. 2005. Effects of grain size of substrate on the growth of a benthic microalgae Nitzschia sp. J. Grad. Sch. Biosp. Sci., Hiroshima Univ., 44, 31-38
  17. Yamamoto, T., K. Tarutani., M. Kawahara and S.J. Oh. 1999. Utilization and excretion of dissolved organic phosphorus by Alexandrium tamarense (Hiroshima Bay strain). J. Fac. Appl. Biol. Sci., Hiroshima Univ., 38, 151-159