• Proceedings of the Korean Society of Sericultural Science Conference
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• 2003.10a
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• pp.145-146
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• 2003

Overproduction of transforming growth factor (TGF)-$\beta$l has been implicated in the pathogenesis of fibrotic diseases. TGF-$\beta$l plays a crucial role in the accumulation of extracellular matrix (ECM) in human and experimental glomerular diseases. However, it remains unclear whether inhibition of TGF- $\beta$l overproduction would suppress TGF- $\beta$l induced ECM accumulation. To inhibit the overproduction of TGF- $\beta$l in experimental glomerulonephritis induced by anti-Thy 1.1 antibody, we introduced antisense oligodeoxynucleotides (ODN) fur TGF- $\beta$l into the nephritic kidney by the HVJ-liposome-mediated gene transfer method. (omitted)

• Journal of the Korea Concrete Institute
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• v.12 no.4
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• pp.113-119
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• 2000

The R/C flat plate system provides architectural flexibility, clear space, reduced building height, simple formwork, which consequently enhance constructibility. One of the serious problems in the flat plate system is brittle punching shear failure due to transfer of shear force and unbalanced moments in column-slab joint. Recently, the flat plate system accompanied with shear walls to resist the lateral loads is applied to high-rise buidings. Although the flat plate system is not considered in design as part of the lateral load-resisting system, it is required that this system keeps the ductile behavior for the lateral displacement of the building. However, it is unclear whether the column-slab joint possesses ductility enough to survive the lateral deformation. The objective of this paper is to investigate the major parameters that influence the ductility of R/C flat plate system by examining the existing experiments on column-slab joint. The effects of gravity load and shear reinforcement on the ductility of the flat plate system are presented.

• Journal of Advanced Marine Engineering and Technology
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• v.22 no.5
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• pp.595-608
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• 1998

A diffuser an important equipment to change kinetic energy into pressure energy has been studied for a long time. Though experimental and theoretical researches habe been done the understanding of energy transfer and detailed mechanism of energy dissipation is unclear. As far as numerical prediction of diffuser flows are concerned various numerical studies have also been done. On the contrary many turbulence models have constraint to the applicability of diffuser-like flows with expansion and streamline curvature. In order to obtain the reliability of k-$\varepsilon$ turbulence model modified combination turbulence models composed of the anisotropic k-$\varepsilon$model modified combination turbulence models composed of the anisotropic k-$\varepsilon$ model with Hanjalic-Launder's preferential normal strain and Pope's vortex stretching mechanism are proposed. The results of the present proposed models prove the fact that the coefficient of pressure and the shear stress are well predicted at the diffuser flow.

• Proceedings of the KSME Conference
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• 2004.04a
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• pp.1613-1618
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• 2004

Diffuser is an important fluid-mechanical equipment to convert kinetic energy into pressure energy. Many of the experimental and theoretical researches have been done in a diffuser but the understanding of energy transfer and detailed mechanism of energy dissipation is unclear. In this study, computations were performed using a numerical method with SIMPLE algorithm for conical diffuser with various diffuser angles and diffuser lengths. Also, we investigated the pressure recovery coefficient in conical diffuser by inserting strut. In this paper, we showed that the strut can cause a rising pressure recovery.

• Genomics & Informatics
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• v.19 no.2
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• pp.18.1-18.8
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• 2021

G protein–coupled receptors (GPCRs), including olfactory receptors, account for the largest group of genes in the human genome and occupy a very important position in signaling systems. Although olfactory receptors, which belong to the broader category of GPCRs, play an important role in monitoring the organism's surroundings, their actual three-dimensional structure has not yet been determined. Therefore, the specific details of the molecular interactions between the receptor and the ligand remain unclear. In this report, the interactions between human olfactory receptor 1A1 and its odorant molecules were simulated using computational methods, and we explored how the chemically simple odorant molecules activate the olfactory receptor.

• Journal of Chest Surgery
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• v.54 no.6
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• pp.535-538
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• 2021

Schwannomas are rare benign tumors that develop in Schwann cells lining peripheral nerves. Schwannomas of the brachial plexus are especially rare, accounting for 5% of all cases. Although several treatments can be considered, the exact method of treatment is unclear owing to the scarcity and sporadic occurrence of schwannomas. Tumor resection is performed in most cases, and nerve damage is inevitable in cases of neuroinvasive schwannoma. In this case series, we present our successful use of transposition of cable-grafted nerves for the treatment of schwannomas. We performed cable-grafted nerve interposition in addition to tumor resection, leading to increased recovery of nerve damage. To relieve postoperative symptoms and minimize sequelae, precise surgical tumor resection followed by nerve interposition using a cable-grafted nerve may be recommended.

• Journal of Korean Tunnelling and Underground Space Association
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• v.19 no.6
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• pp.887-903
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• 2017

Lots of shallow tunnels are constructed in the mountainous areas where the stress distribution in the ground around tunnel is not simple, also the impact of stress conditions on the longitudinal load transfer characteristics is unclear. The tunnel construction methods and the ground conditions would also affect the longitudinal load transfer characteristics which would be dependant on the displacement patterns of tunnel face. Therefore, in this study, the slope of the ground surface was varied in $0^{\circ}$, $10^{\circ}$, $20^{\circ}$, $30^{\circ}$, and the longitudinal load transfer depended on the deformation conditions of tunnelface (that were maximum deformation on the top, constant deformation, and maximum deformation on the bottom), and the stress distribution at tunnelface. As results, when the tunnelface deformed, the earth presure on the tunnelface decreased and the load at tunnel crown increased. The load transferred on the crown was influenced by the earth presure on tunnel face. Smaller load would be transfered to the wide areas when the slope of ground surface decreased. When the slope of ground surface became larger, the longitudinal load transfer would be smaller and would be concentrated on tunnelface, In addition, the shape of the transferred load distribution in the longitudinal direction was dependant on the deformation shape of tunnelface. The deformation shape of tunnelface and stress conditions in longitudinal sections would affect the shape and the magnitude of the load transfer in the longitudinal directions.

• The Journal of Korean Obstetrics and Gynecology
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• v.35 no.2
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• pp.28-41
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• 2022

Objectives: The purpose of this study is to investigate the effectiveness of moxibustion in infertility with In Vitro Fertilization and Embryo Transfer (IVF-ET). Methods: We searched 8 databases (Embase, PubMed, CiNii, CNKI, OASIS, ScienceOn, KMBASE, KISS)to identify eligible studies published before 2021 Oct. We included randomized controlled clinical trials (RCTs) using moxibustion in infertility with IVF-ET. The methodological quality of each RCT was assessed by the Cochrane risk of bias tool. Results: Two RCT studies were eligible in our review. The overall risk of bias was evaluated as unclear. The meta-analysis of 2 trials indicated that favorable results for the use of moxibustion with IVF-ET. Conclusions: This systematic review and meta-analysis of clinical trials suggests that moxibustion with IVF-ET can effect on Infertility patients. However, because of studies included analysis was biased due to unclear risk of bias and unreliable study design, future RCT studies and additional Meta-Analysis are needed to judge the supplementary treatment role of moxibustion in infertility with IVF-ET.

• Journal of Embryo Transfer
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• v.26 no.2
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• pp.111-115
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• 2011

In general, zona pellucida (ZP) of the blastocyst has to be removed first, then either isolated the inner cell mass (ICM) or ZP-removed whole blastocyst, which is then cultured on the feeder layer to induce ICM outgrowth for the generation of embryonic stem cells (ESC). However, it is unclear whether ICM isolation before seeding on feeder layer is beneficial or not because the interaction between ICM and trophoblasts may affect cellular growth and/or pluripotency during the culture on the feeder. In the present study, two ZP removal methods (mechanically by splitting with a 28-gauge needle versus chemically by the treatment of acid-Tyrode's solution) and two ICM isolation methods (ZP-free whole blastocyst seeding versus mechanical isolation of ICM) were evaluated for the efficient isolation and culture of putative parthenogenetic bovine ESC. The number of maintained outgrown colonies was counted in each experimental group. As the result, mechanical removal of ZP with a needle and followed by whole ZP-free blastocyst seeding on feeder cells tended to attach more on the feeder layer and resulted in more outgrown colonies with its simple and less time-costing benefits. Currently we are generating ESC lines in HanWoo cattle by using this method for initial outgrowth of the parthenogenetic bovine blastocysts.

• Journal of Embryo Transfer
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• v.15 no.2
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• pp.167-173
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• 2000

The principal objective of this study was to clone transgenic embryos in order to improve the efficiency of transgenic animal production by the combination of microinjection and nuclear transplantation techniques. Mature female New Zealand White rabbits were superovulated by eCG and hCG treatments, fllowed by natural mating. Zygotes were collected from the oviducts at 18∼22 h after hCG injection by flushing with D-PBS containing 5% fetal calf serum(FCS). Two to three picoliters of green fluorescent protein(GFP) gene wa microinjected into male pronucleus. The foreign gene-injected zygotes were cultured in TCM-199 or RD medium containing 10% FCS with a monolayer of rabbit oviductal epithelial cells in a 5% CO2 incubator. The morulae expressing GFP gene were selected and their blastomeres were separated for the use of nuclear donor. Following nuclear transplantation of fluorescence-positive morula stage blastomeres, 13 (21.3%) out of 61 fused oocytes developed to blastocyst stage and all of the cloned blastocysts expressed GFP. The results indicate that the screening of transgene in rabbit embryos by GFP detection could be a promisible method for the preselection of transgenic embryos. Also the cloning of preselected transgenic embryos by nuclear transplantatin could be efficiently applied to the multiple production of transgenic animals.