• Journal of Microbiology
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• v.42 no.4
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• pp.319-327
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• 2004

An additional amylase, besides the typical $\alpha-amylase,$ was detected for the first time in the cytoplasm of B. subtilis SUH4-2, an isolate from Korean soil. The corresponding gene (bbmA) encoded a malto­genic amylase (MAase) and its sequence was almost identical to the yvdF gene of B. subtilis 168, whose function was unknown. Southern blot analysis using bbmA as the probe indicated that this gene was ubiquitous among various B. subtilis strains. In an effort to understand the physiological function of the bbmA gene in B. subtilis, the expression pattern of the gene was monitored by measuring the $\beta-galactosidase$ activity produced from the bbmA promoter fused to the amino terminus of the lacZ struc­tural gene, which was then integrated into the amyE locus on the B. subtilis 168 chromosome. The pro­moter was induced during the mid-log phase and fully expressed at the early stationary phase in defined media containing $\beta--cyclodextrin\;(\beta-CD),$ maltose, or starch. On the other hand, it was kept repressed in the presence of glucose, fructose, sucrose, or glycerol, suggesting that catabolite repression might be involved in the expression of the gene. Production of the $\beta-CD$ hydrolyzing activity was impaired by the spo0A mutation in B. subtilis 168, indicating the involvement of an additional regu­latory system exerting control on the promoter. Inactivation of yvdF resulted in a significant decrease of the $\beta-CD$ hydrolyzing activity, if not all. This result implied the presence of an additional enzyme(s) that is capable of hydrolyzing $\beta-CD$ in B. subtilis 168. Based on the results, MAase encoded by bbmA is likely to be involved in maltose and $\beta-CD$ utilization when other sugars, which are readily usable as an energy source, are not available during the stationary phase.

• Environmental Analysis Health and Toxicology
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• v.17 no.2
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• pp.147-160
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• 2002

Volatile organic compounds (VOCs) are an important public health issue in Korea and many important questions remain to be addressed with respect to assessing exposure to these compounds. Because they are ubiquitous and highly volatile, special techniques must be applied in their analytic determination Valid Personal exposure assessment methods are needed to evaluate exposure frequency, duration and intensity, as well as their relationship to personal exposure characteristics. Biological monitoring is also important since it may contribute significantly in risk assessment by allowing the estimation of effective absorbed doses. This study was on ducted to establish the environmental measurement, personal dosimetry and biological monitoring methods for VOCs. These methods are needed to compare blood, urinary and exhalation breath VOC levels and to provide tools for risk assessment of VOC exposure. Passive monitors (badge type) and a active samplers (trap) for the VOCs collection were used for air sampling. Methods development included determining the minimum detectable amounts of VOCs in each media, as well as evaluating collection methods and developing analytical procedures. Method reliability was assessed by determining breakthrough volumes and comparing results between laboratories and with other methods. A total capacity of trap used in this study was 60ι. Although variable by compound, the average breakthrough was 20％. Also, there was no loss of compounds in trap even if keep for 45 day in -7$0^{\circ}C$. The recovery of active and passive methods was 69％ ~ 126％ and method detection limit was 0.24 $\mu\textrm{g}$/trap and 0.07 $\mu\textrm{g}$/badge. There was no statistical difference (P > 0.05) between active and passive methods.

• Journal of Broadcast Engineering
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• v.9 no.3
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• pp.225-235
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• 2004

In this paper, we propose a new stereoscopic video coding approach for heterogeneous consumer devices by exploiting the concept of spatio-temporal scalability. The proposed method uses MPEG-2 standard for coding the left or main sequence and an enhanced compatible coding scheme for predicting the P- and B-type of frames of the right or auxiliary sequence. The enhanced compatible coding scheme predicts matching block by interpolating both two forward and backward motion predicted macroblocks and disparity predicted macroblock. To provide flexible stereo video service, we define both a temporally scalable layer and a spatially scalable layer for each eye-view. The experimental results show the efficiency of proposed coding scheme by comparison with already known methods and the advantages of disparity estimation in terms of scalability overhead. According to the experimental results, we expect the proposed functionalities will play a key role in establishing highly flexible stereo video service for ubiquitous computing environment where devices and network connections are heterogeneous.

• Journal of Microbiology and Biotechnology
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• v.29 no.11
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• pp.1830-1840
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• 2019

Loliolide is one of the most ubiquitous monoterpenoid compounds found in algae, and its potential therapeutic effect on various dermatological conditions via agent-induced biological functions, including anti-oxidative and anti-apoptotic properties, was demonstrated. Here, we investigated the effects of loliolide on hair growth in dermal papilla (DP) cells, the main components regulating hair growth and loss conditions. For this purpose, we used a three-dimensional (3D) DP spheroid model that mimics the in vivo hair follicle system. Biochemical assays showed that low doses of loliolide increased the viability and size of 3D DP spheroids in a dose-dependent manner. This result correlated with increases in expression levels of hair growth-related autocrine factors including VEGF, IGF-1, and KGF. Immunoblotting and luciferase-reporter assays further revealed that loliolide induced AKT phosphorylation, and this effect led to stabilization of β-catenin, which plays a crucial role in the hair-inductive properties of DP cells. Further experiments showed that loliolide increased the expression levels of the DP signature genes, ALP, BMP2, VCAN, and HEY1. Furthermore, conditioned media from loliolide-treated DP spheroids significantly enhanced proliferation and the expression of hair growth regulatory genes in keratinocytes. These results suggested that loliolide could function in the hair growth inductivity of DP cells via the AKT/β-catenin signaling pathway.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.21 no.11
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• pp.2145-2152
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• 2017

As the ubiquitous paradigm that allows users to access the Internet anytime and anywhere is expanding, the expectation and interest of the Internet is increasing. As interest in the Internet has increased, interest in open source hardware has also increased. As a result, the maker, who is a creator who actually uses the digital production tools to create a product that he thought, is emerging as a new trend. As these makers shared how to make products, the 'maker movement', an open source manufacturing movement, spread. In the case of the domestic market, the government's policy support is also stimulating the maker movement. However, compared to developed countries, there is not enough awareness and environment of maker culture, and only open platform hardware and software are used without special education / development equipment or kit for maker.

• The Korean Journal of Mycology
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• v.46 no.3
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• pp.213-225
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• 2018

Trichoderma (Hypocreaceae) is one of the most ubiquitous genera worldwide. This genus has an excellent ability to adapt to diverse environments, even under poor nutritional conditions, such as in freshwater. However, little is known about the diversity of Trichoderma species in freshwater environments. In this study, we isolated diverse fungal strains from algae, plant litter, and soil sediment in streams in Korea. The strains were identified based on molecular phylogenetic analyses of internal transcribed spacer (ITS) rDNA and translation elongation factor 1 ($TEF1{\alpha}$) sequences. We also investigated their morphological characteristics by microscopic observation and determination of cultural features on different media. As a result, six Trichoderma species were found in Korea: T. afroharzianum, T. capillare, T. guizhouense, T. paraviridescens, T. reesei, and T. saturnisporum. Interestingly, T. paraviridescens showed both cellulose activity and hypoxia stress tolerance phenotypes, indicating its role as a decomposer in freshwater ecosystems. Our study revealed that freshwater environment could be a good candidate for investigating the species diversity of Trichoderma.

• The Journal of Korean Association of Computer Education
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• v.17 no.3
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• pp.1-10
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• 2014

Smartphone addiction and internet addiction cause a serious negative social problem due to the spread of the new media which is combined with the ubiquitous function. To evaluate the degree of Internet addiction and smartphone addiction of college students in the nearby areas, K-scale of internet addiction self-diagnosis scale and S-scale of smartphone addiction self-diagnosis scale for Korean adults were applied. This study aims to develop the survey questions, which get the basic internet patterns and access the web site, and to find the hidden addicts that is not found by general K-scale and S-scale using computers and smartphones. The result shows that a method of addiction diagnosis using the tolerance degree which is calculated by smart phone's internet main activities and using time(anticipated and real time) apart from K-scale and S-scale. So hidden internet and smartphone addicts could be found through the SPSS statistical analysis program.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.47 no.7
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• pp.135-142
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• 2010

Wireless mesh network, which is an access network technology, adopts ubiquitous features of ad-hoc network that includes capabilities of self-configuration and self-management. This paper proposes a scheme which enables nodes along route in wireless mesh network to authenticate data and verify data integrity. The scheme distinguishes infra-node, which is a network device used to form mesh network, and user node in ad-hoc network, which operates functions as a sender, receiver or relayer, to deploy different authentication scheme. That is, hop-based authentication scheme along route forming wireless backbone differs from authentication scheme for user nodes in route over MANET. The proposed scheme is less complex than previously proposed schemes from the repects of security setup procedures and managements. In addition, the scheme is able to reduce transmission delay from a source to a destination owing to fast authentication over wireless backbone.

• The Journal of the Korea Contents Association
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• v.8 no.4
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• pp.264-272
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• 2008

In ubiquitous geographic information environment, the users are provided with geographic information anywhere and any time on their needs by any types of devices and communication media. The unique location ID, ePosition, is a new technology to support UBGI environment, using a logical location ID instead of physical location of a point of interest. Domain names of plural ePosition servers, where location information with its ePosition is stored, needs to be registered in DNS for some service through Internet. For better ePosition service of the type of public service like email service, a set of DNS resource records can be newly defined. This paper suggests a new DNS resource record EPO for implementation of the ePosition service through Internet.

• Journal of the Ergonomics Society of Korea
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• v.29 no.4
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• pp.585-593
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• 2010

Recently, we designed an interactive virtual space for the multi-purpose hall in Songdo Future City, located in Incheon, Korea. The goal of the design is to make a virtual space that is flexible and can be adjusted thanks to its unfixed seats in order to accommodate different and unspecified audience sizes. Virtual images are interactively adjusted according to the distance, position and size of audiences, information about which is detected by 9 photo sensors. To increase the sense of immersion, intensity and reality, we utilized the technology of scent display that can create appropriate scents to match the images on the screen. The intensity and persistence of scents were determined by the size, distance and position of audiences. The virtual image contains background images and reactive images. The background images repeatedly project images of spring, summer, autumn and winter. The reactive images consist of small portraits or pictures or icons that define or characterize the season types, and these are added to the background image according to the distance, position and size of the audiences.