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http://dx.doi.org/10.4489/KJM.20180027

First Report of Six Trichoderma Species Isolated from Freshwater Environment in Korea  

Goh, Jaeduk (Fungal Resources Research Division, Nakdonggang National Institute of Biological Resources)
Nam, Bora (Department of Biology, Kunsan National University)
Lee, Jae Sung (Department of Biology, Kunsan National University)
Mun, Hye Yeon (Fungal Resources Research Division, Nakdonggang National Institute of Biological Resources)
Oh, Yoosun (Fungal Resources Research Division, Nakdonggang National Institute of Biological Resources)
Lee, Hyang Burm (Division of Food Technology, Biotechnology and Agrochemistry, Chonnam National University)
Chung, Namil (Fungal Resources Research Division, Nakdonggang National Institute of Biological Resources)
Choi, Young-Joon (Department of Biology, Kunsan National University)
Publication Information
The Korean Journal of Mycology / v.46, no.3, 2018 , pp. 213-225 More about this Journal
Abstract
Trichoderma (Hypocreaceae) is one of the most ubiquitous genera worldwide. This genus has an excellent ability to adapt to diverse environments, even under poor nutritional conditions, such as in freshwater. However, little is known about the diversity of Trichoderma species in freshwater environments. In this study, we isolated diverse fungal strains from algae, plant litter, and soil sediment in streams in Korea. The strains were identified based on molecular phylogenetic analyses of internal transcribed spacer (ITS) rDNA and translation elongation factor 1 ($TEF1{\alpha}$) sequences. We also investigated their morphological characteristics by microscopic observation and determination of cultural features on different media. As a result, six Trichoderma species were found in Korea: T. afroharzianum, T. capillare, T. guizhouense, T. paraviridescens, T. reesei, and T. saturnisporum. Interestingly, T. paraviridescens showed both cellulose activity and hypoxia stress tolerance phenotypes, indicating its role as a decomposer in freshwater ecosystems. Our study revealed that freshwater environment could be a good candidate for investigating the species diversity of Trichoderma.
Keywords
Freshwater; Extracellular enzymes; ITS rDNA; Plant litter; $TEF1{\alpha}$;
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