• Title/Summary/Keyword: u-map

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Cloning and Characterization of a Methionine Aminopeptidase (MAP) Gene from Tetragenococcus halophilus CY54 Isolated from Myeolchi-Jeotgal

  • Tae Jin Kim;Min Jae Kim;Yun Ji Kang;Ji Yeon Yoo;Jeong Hwan Kim
    • Microbiology and Biotechnology Letters
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    • v.51 no.1
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    • pp.26-31
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    • 2023
  • A map gene encoding methionyl-specific aminopeptidase (MAP; EC 3.4.11.18) was cloned from Tetragenococcus halophilus CY54. Translated amino acid sequence of CY54 MAP showed high similarities with those from Enterococcus faecalis (83.8%) and Streptococcus salivarius (62.2%) but low similarities with MAPs from Lactobacillus and Lactococcus genera. The map gene was overexpressed in E. coli BL21(DE3) using pET26b(+),pET26b(+), and the recombinant MAP was purified by using an Ni-NTA column. The size of recombinant MAP was 29 kDa as determined by SDS-PAGE. The optimum pH and temperature of CY54 MAP were pH 5.0 and 60℃, respectively. The activity of CY54 MAP was most significantly increased by Co2+ ion (159%), and showed the highest activity at 12% NaCl. Km and Vmax were 0.64 ± 0.006 mM and 10.12 ± 0.014 U/mg protein, respectively when met-pNA was used as the substrate. This is the first report on a MAP from Tetragenococcus species.

Virtual City System Based on 3D-Web GIS for U-City Construction (U-City 구현을 위한 3D-Web GIS 기반의 가상도시 시스템)

  • Jo, Byung-Wan;Lee, Yun-Sung;Yoon, Kwang-Won;Park, Jung-Hun
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.25 no.5
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    • pp.389-395
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    • 2012
  • U-City has been promoted nation-wide by development of recent IT technology. This paper studied the concept of 3D-virtual city in order to realize the current Ubiquitous City(U-City) efficiently, and to manage all the RFID/USN monitoring data in the real U-City. 3D-Virtual City is the concept of the reproduction of real world U-City, for embodying Ubiquitous technology while using Digital map, satellite image, VRML(Virtual Reality Modeling Language). U&V-City is the four-dimensional future city that real-time wire/wireless communication network and 3D-web GIS shall be connected that massive database, intelligent service be perceived through employing EAI(External Authoring Interface) that provides HTML&JAVA, and interface for efficient removal/process of massive information/ service and also by employing GPS/LBS/Navigation in support of world-wide orientation concept, and RTLS(Real Time Location System).

D-정책과 복수휴가를 갖는 MAP/G/1 대기행렬시스템의 고객수분석

  • Jeon, Sang-Hun;Lee, Ho-U
    • Proceedings of the Korean Operations and Management Science Society Conference
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    • 2005.05a
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    • pp.874-880
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    • 2005
  • 본 논문에서는 D-정책과 복수휴가를 갖는 MAP/G/1 대기행렬시스템의 고객수를 분석한다. 고객도착은 마코비안 도착과정(MAP)을 따른다. 서어버는 바쁜기간 종료 직후 휴가를 떠나고 휴가에서 돌아온 직후의 시스템 총일량이 D를 초과할 때까지 휴가를 반복한다. 만일, 휴가에서 돌아온 직후의 시스템 총일량이 D를 초과하면, 서어버는 즉시 서비스를 시작한다. 본 논문에서는 임의의 이탈시점 고객수분포를 유도하고, 이를 이용하여 임의시점 고객수분포와 평균고객수를 유도한다. 또한, 동일한 조건하에 D-정책과 복수휴가를 갖는 M/G/1 대기행렬시스템의 평균고객수와의 차이를 비교해 본다.

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Development of Flood Map Using Geographic Information System (GIS기반 홍수예측지도의 개발)

  • Kim Sang-Ho;Kim Han-Joong;Lee Nam-Ho;Kim Seong-Joon
    • Proceedings of the KSRS Conference
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    • 2006.03a
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    • pp.36-40
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    • 2006
  • The objective of the study is to develop a GIS-based flood map. Hydraulic model (HEC-RAS) is linked with hydrologic model (HEC-HMS) for flood map. Geospatial data processors, HEC-GeoHMS and HEC-GeoRAS, are used for operating HEC-HMS and HEC-RAS. HEC-HMS was calibrated and validated at the Hwa-Ong watershed. HEC-HMS was used for calculating runoff from the Hwa-Ong watershed which consisted of Nam-Yang, Ja-An, U-Eun river sub-watersheds, and HEC-RAS was applied and validated for river flow routing at the Hwa-Ong watershed. The simulated results from HEC-HMS and HEC-RAS were reasonably good compared with the observed data. HEC-RAS and HEC-HMS were applied to simulate flooding from probability rainfall at the Hwa-Ong watershed, and the simulated result was used to develop a flood map. Flood map developed in this study will be used for mitigating and predicting the flood damages.

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Automatic Generation of Land Cover Map Using Residual U-Net (Residual U-Net을 이용한 토지피복지도 자동 제작 연구)

  • Yoo, Su Hong;Lee, Ji Sang;Bae, Jun Su;Sohn, Hong Gyoo
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.40 no.5
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    • pp.535-546
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    • 2020
  • Land cover maps are derived from satellite and aerial images by the Ministry of Environment for the entire Korea since 1998. Even with their wide application in many sectors, their usage in research community is limited. The main reason for this is the map compilation cycle varies too much over the different regions. The situation requires us a new and quicker methodology for generating land cover maps. This study was conducted to automatically generate land cover map using aerial ortho-images and Landsat 8 satellite images. The input aerial and Landsat 8 image data were trained by Residual U-Net, one of the deep learning-based segmentation techniques. Study was carried out by dividing three groups. First and second group include part of level-II (medium) categories and third uses group level-III (large) classification category defined in land cover map. In the first group, the results using all 7 classes showed 86.6 % of classification accuracy The other two groups, which include level-II class, showed 71 % of classification accuracy. Based on the results of the study, the deep learning-based research for generating automatic level-III classification was presented.

Depth Map Extraction from the Single Image Using Pix2Pix Model (Pix2Pix 모델을 활용한 단일 영상의 깊이맵 추출)

  • Gang, Su Myung;Lee, Joon Jae
    • Journal of Korea Multimedia Society
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    • v.22 no.5
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    • pp.547-557
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    • 2019
  • To extract the depth map from a single image, a number of CNN-based deep learning methods have been performed in recent research. In this study, the GAN structure of Pix2Pix is maintained. this model allows to converge well, because it has the structure of the generator and the discriminator. But the convolution in this model takes a long time to compute. So we change the convolution form in the generator to a depthwise convolution to improve the speed while preserving the result. Thus, the seven down-sizing convolutional hidden layers in the generator U-Net are changed to depthwise convolution. This type of convolution decreases the number of parameters, and also speeds up computation time. The proposed model shows similar depth map prediction results as in the case of the existing structure, and the computation time in case of a inference is decreased by 64%.

Application Development and Performance Analysis of Smartphone-based QR Code Interpreter (스마트폰 기반의 QR코드 해석기 성능분석 및 응용개발)

  • Park, Chan-Jung;Hyun, Jung-Suk
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.13 no.10
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    • pp.2242-2250
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    • 2009
  • Recently, with the advance of Ubiquitous era, the types of services become diverse. Especially, due to the rapid development of mobile technology, the new functions of mobile phones are added and the new applications of mobile phones are developed actively. Among the various applications related to mobile phones, 2 dimensional barcode-based applications are increasing. 2 dimensional barcode is mostly used for the management of past record. However, by combing 2 dimensional barcode with mobile phones, the application areas of 2 dimensional barcode are expanded to the means of publicity for education, tourism, and festivals. In this paper, we develop a QR code decoder running on smartphones, which connects on-line and off-line. In addition, we modify our decoder by detecting the point for performance enhancement based on TRIZ. We compare our decoder with an open-source based decoder in terms of the code size of decoding and the speed of decoding in order to prove that our decoder has a better performance than the other. Finally, we introduce two applications: u-map and u-pamphlet as QR code applications.

Effect of EGCG on Expression of Neurogenin 3 via the MAP Kinase Signaling Pathway in AR42J Cells, a Rat Pancreatic Tumor Cell Line (녹차 카테킨, Epigallocathechin Gallate (EGCG)의 흰쥐췌장종양 선 세포 AR42J의 MAP Kinase 세포 신호전달 기전을 통한 Neurogenin 3 발현에 미치는 영향)

  • Kim, Sung-Ok;Choe, Won-Kyung
    • Journal of Nutrition and Health
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    • v.44 no.3
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    • pp.196-202
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    • 2011
  • Epigallocatechin gallate (EGCG), or epigallocatechin 3-gallate, is the ester of epigallocatechin and gallic acid, and is a type of catechin. EGCG may be therapeutic for many disorders including diabetics and some types of cancer. However it is unknown whether EGCG can induce transdifferentiation of pancreatic cells in pancreatitis. The aim of this study was to investigate the effects of EGCG on the expression of pancreatic regenerating related markers in pancreatic AR42J cells, a model of pancreatic progenitor cells. AR42J cells, differentiated with betacellulin and activin A, were cultured with/without EGCG in a time-dependent manner. Cell growth rate, levels of mRNA, and protein expression were examined with the MTT assay, quantitative PCR, and Western blots, respectively. The results showed that AR42J cell growth rates were inhibited by EGCG in a dose-dependent manner. mRNA and protein expression of amylase, insulin and neurogenin 3 (ngn 3) increased in AR42J cells treated with EGCG. Additionally, we demonstrated that the signal transduction pathway of mitogen-activated protein (MAP) kinase is active in EGCG-treated AR42J cells. ERK and JNK phosphorylation decreased in cells treated with EGCG but not p38 phosphorylation. Activation of the p38 MAP kinase pathway was confirmed by specific MAP kinase pathways inhibitors: U0126 for ERK, SP600126 for JNK, and SB203580 for p38. Activated p38 phosphorylation was inhibited by the specific p38 inhibitor SB203580 but p38 phosphorylation was inhibited with increased EGCG treatment. The ERK and JNK MAP kinase pathways were not affected by EGCG treatment. Although further studies are needed, these results suggest that EGCG affects the induction of pancreatic cell regeneration by increasing the ngn 3 protein and mRNA expression and activating the p38 MAP kinase pathway.

NEHARI MANIFOLD AND MULTIPLICITY RESULTS FOR A CLASS OF FRACTIONAL BOUNDARY VALUE PROBLEMS WITH p-LAPLACIAN

  • Ghanmi, Abdeljabbar;Zhang, Ziheng
    • Bulletin of the Korean Mathematical Society
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    • v.56 no.5
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    • pp.1297-1314
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    • 2019
  • In this work, we investigate the following fractional boundary value problems $$\{_tD^{\alpha}_T({\mid}_0D^{\alpha}_t(u(t)){\mid}^{p-2}_0D^{\alpha}_tu(t))\\={\nabla}W(t,u(t))+{\lambda}g(t){\mid}u(t){\mid}^{q-2}u(t),\;t{\in}(0,T),\\u(0)=u(T)=0,$$ where ${\nabla}W(t,u)$ is the gradient of W(t, u) at u and $W{\in}C([0,T]{\times}{\mathbb{R}}^n,{\mathbb{R}})$ is homogeneous of degree r, ${\lambda}$ is a positive parameter, $g{\in}C([0,T])$, 1 < r < p < q and ${\frac{1}{p}}<{\alpha}<1$. Using the Fibering map and Nehari manifold, for some positive constant ${\lambda}_0$ such that $0<{\lambda}<{\lambda}_0$, we prove the existence of at least two non-trivial solutions