• Journal of Life Science
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• v.24 no.12
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• pp.1330-1338
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• 2014

In Mycobacterium tuberculosis H37Rv 16 adenylyl cyclase (AC) genes have been identified, while 10 AC genes have been found in non-pathogenic Mycobacterium smegmatis. Expression of 6 AC genes (MSMEG_0218, MSMEG_3243, MSMEG_3780, MSMEG_4279, MSMEG_4477, and MSMEG_6154) among 10 AC genes in M. smegmatis was increased when M. smegmatis was subjected to hypoxic growth conditions. On the other hand, only MSMEG_3780 and MSMEG_4279 were slightly induced in the presence of NO. The cAMP levels in cells and culture media were 450- and 9.8-fold increased, respectively, when M. smegmatis was grown under hypoxic conditions relative to those grown aerobically. Intracellular levels of cAMP were increased 5.8-fold on the exposure to NO. The DevSR two-component system is known to be involved in the induction of many genes whose expression is induced under hypoxic conditions and in the presence of NO. Expression of 6 hypoxically induced AC genes was observed to be induced in a devR deletion mutant grown under hypoxic conditions, indicating that the induction of the 6 AC genes under hypoxic conditions is independent of the DevSR two-component system. In order to identify a trans-acting regulatory element that is pertinent in the hypoxic induction of MSMEG_3780, ligand-fishing analysis was performed using the upstream DNA of MSMEG_3780 and MSMEG_5136 protein was identified.

• Korean Journal of Microbiology
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• v.48 no.3
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• pp.187-191
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• 2012

Two-component system (TCS)-based bacterial zinc and copper biosensors, in which green fluorescent protein (GFP) is expressed under the control of zraP and cusC promoter in ZraS/R and CusS/R TCS, were evaluated in artificial wastewater. Bacterial biosensors developed in this study efficiently expressed GFP by the recognition of $Zn^{2+}$ and $Cu^{2+}$ in artificial wastewater. Secondly, TCS-based zinc and copper removing bacteria with the peptide displayed on cell surface were examined in artificial wastewater. Zinc and copper removing bacteria expressed the peptide as a fusion protein such as OmpC-ZBP (zinc binding peptide) and OmpC-CBP (copper binding peptide) on the cell surface when sensing exogenous $Zn^{2+}$ and $Cu^{2+}$ through ZraS/R and CusS/R TCS. The recombinant cell expressing metal-adsorbing peptide could efficiently remove copper and zinc (15 and 18 mg/g dry cell weight, respectively) in artificial wastewater. Therefore, it was demonstrated that the TCS-based recombinant cell for the recognition or removal of heavy metal functions well in artificial wastewater environment.

• Journal of Hydrogen and New Energy
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• v.16 no.3
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• pp.209-218
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• 2005

To find a suitable metal component in oxygen carrier particles for chemical-looping hydrogen generation system(CLH), oxygen transfer capacities of metal components were compared and Ni has been selected as the best metal component. The proper operating conditions to achieve high hydrogen generation rate have been investigated based on the chemical-equilibrium composition analysis for water splitting reactor. Moreover, suitable compositions of syngas from gasifier of heavy residue to achieve high energy efficiency have been investigated by calculation of heat of reaction. Based on the selected operating conditions, the best configuration of two interconnected fluidized beds system for the chemical-looping hydrogen generator has been investigated as well.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2006.11a
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• pp.898-902
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• 2006

The motion of a system composed of a plate, constant springs and varying dampers is considered when the system is subject to harmonic force. Letting the frequencies of harmonic force and damper variation ${\Large f}_1\;and\;{\Large f}_2$ respectively, the displacement at the center of the plate has the strongest component at frequency ${\Large f}_1$. The angular displacement of the plate has strong components at ${\Large f}_1-{\Large f}_2$ and the natural frequency of the rotational mode of the system. If these two frequencies coincide, the plate oscillates with almost single frequency and a large amplitude. Part of these simulation results are proved analytically.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.16 no.9 s.114
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• pp.958-963
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• 2006

The motion of a system composed of a plate, constant springs and varying dampers is considered when the system is subject to harmonic force. Letting the frequencies of harmonic force and damper variation $f_1\;and\;f_2$, respectively, the displacement at the center of the plate has the strongest component at frequency $f_1$. The angular displacement of the plate has strong components at $f_1-f_2$ and the natural frequency of the rotational mode of the system. If these two frequencies coincide, the plate oscillates with almost single frequency and a large amplitude. These results can be applied to development of a moment shaker with low frequencies.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2006.05a
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• pp.81-85
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• 2006

The motion of a system composed of a plate, constant springs and varying dampers is considered when the system is subject to harmonic force. Letting the frequencies of harmonic force and damper variation ${\Large f}_1\;and\;{\Large f}_2$, respectively, the displacement at the center of the plate has the strongest component at frequency ${\Large f}_1$. The angular displacement of the plate has strong components at ${\Large f}_1-{\Large f}_2$, and the natural frequency of the rotational mode of the system. If these two frequencies coincide, the plate oscillates with almost single frequency and a large amplitude. These results can be applied to development of a moment shatter with low frequencies.

• Journal of KSNVE
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• v.2 no.2
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• pp.125-134
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• 1992

In this study, ti identify the dynamic characteristics of automobile steering system which consists of many components and joints, each component combined structure was analyzed using commercial structural package, ANSYS. And, the finite element method for each component and modeling method of several joints universal joint, bolt joint, bearing, etc. were studied. On the other hand, the experimental modal analysis was performed to compare with the results of the finite element analysis and joint modeling. The result shows very close agreement between two analysis. Also, it was found that the steeing column used in this experiment does not effect the low frequency mode of entire system. In addition, we found that constraint equations need to be considered in modeling universal joint. Since the stiffness effect of Urethane around wheel could be ignored, it can be modeled only with mass effect. In the end, it was found that dynamic characteristics of the entire steerintg system depends mainly upon the wheel characteristics.

• Journal of The Korean Society of Agricultural Engineers
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• v.49 no.1
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• pp.101-109
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• 2007

The purpose of this study is to improve ability of GASS using diverse communication layers of material and information. GASS is a dynamic system simulator developed for analyzing complex agricultural system. However, it had two difficulties which are inefficient modeling of information transmission and complicated implementation of material transmission. This paper proposes dual communication layers which consist of material and information to overcome the hardship and applies intake tower component which controls amount of irrigation using water requirement in paddy to an irrigation system model. The application focused on available information communication and correctable material communication.

• 제어로봇시스템학회:학술대회논문집
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• 1987.10b
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• pp.101-104
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• 1987

This paper shows the robot-vision system which consists of robot, vision system, single board computer and IBM-PC. IBM-PC based system has a great flexibility in expansion for a vision system interfacing. Easy human interfacing and great calculation ability are the benefits of this system. It was carried to interface between each component. The calibration between two coordinate systems is studied. The robot language for robot-vision system was written in "C" language. User also can write job program in "C" language in which the robot and vision related functions reside in the library.side in the library.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.33 no.11
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• pp.1288-1293
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• 2009

This paper discusses about modeling method to simulate a nonlinear behavior like sliding or rocking of two stacked body system under earthquake condition. A double body system design can be an option to reduce seismic response of a component in comparison to a single body system for free standing structures. Therefore, according to the priority of components, the structure is to be designed by proper ratio of partition in their height for improvement of seismic capability and structural integrity. Nonlinear modeling and analysis using simple rigid body and dynamic system has been performed to check the trend in such cases. As a result, one of the two bodies can be chosen to reduce the seismic response from energy absorption of the other one by appropriate application of friction ratios not only in slip-slip condition but in slip-rock condition.