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http://dx.doi.org/10.7845/kjm.2012.039

Heavy Metal Detection and Removal in Artificial Wastewater Using Two-Component System Based Recombinant Bacteria  

Ravikumar, Sambandam (School of Chemical Engineering and Bioengineering, University of Ulsan)
Hong, Soon-Ho (School of Chemical Engineering and Bioengineering, University of Ulsan)
Yoo, Ik-Keun (School of Chemical Engineering and Bioengineering, University of Ulsan)
Publication Information
Korean Journal of Microbiology / v.48, no.3, 2012 , pp. 187-191 More about this Journal
Abstract
Two-component system (TCS)-based bacterial zinc and copper biosensors, in which green fluorescent protein (GFP) is expressed under the control of zraP and cusC promoter in ZraS/R and CusS/R TCS, were evaluated in artificial wastewater. Bacterial biosensors developed in this study efficiently expressed GFP by the recognition of $Zn^{2+}$ and $Cu^{2+}$ in artificial wastewater. Secondly, TCS-based zinc and copper removing bacteria with the peptide displayed on cell surface were examined in artificial wastewater. Zinc and copper removing bacteria expressed the peptide as a fusion protein such as OmpC-ZBP (zinc binding peptide) and OmpC-CBP (copper binding peptide) on the cell surface when sensing exogenous $Zn^{2+}$ and $Cu^{2+}$ through ZraS/R and CusS/R TCS. The recombinant cell expressing metal-adsorbing peptide could efficiently remove copper and zinc (15 and 18 mg/g dry cell weight, respectively) in artificial wastewater. Therefore, it was demonstrated that the TCS-based recombinant cell for the recognition or removal of heavy metal functions well in artificial wastewater environment.
Keywords
Escherichia coli; artificial wastewater; biosensor; copper; zinc;
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1 Bae, W., Wu, C.H., Kostal, J., Mulchandani, A., and Chen, W. 2003. Enhanced mercury biosorption by bacterial cells with surfacedisplayed MerR. Appl. Environ. Microbiol. 69, 3176-3180.   DOI   ScienceOn
2 Behera, S.K., Rene, E.R., and Murthy, D.V.S. 2008. Effect of hydraulic retention time and initial nitrate concentration on the performance of an up-flow anoxic bioreactor: A factorial design study. Indian Chem. Eng. 50, 27-33.
3 Biran, I., Babai, R., Levcov, K., Rishpon, J., and Ron, E.Z. 2000. Online and in situ monitoring of environmental pollutants: electrochemical biosensing of cadmium. Environ. Microbiol. 2, 285-290.   DOI   ScienceOn
4 Falchuk, K.H., Hilt, K.L., and Vallee, B.L. 1998. Determination of zinc in biological samples by atomic absorption spectrometry. Method Enzymol. 158, 422-434.
5 Harvey, B.R., Georgiou, G., Hayhurst, A., Jeong, K.J., Iverson, B.L., and Rogers, G.K. 2004. Anchored periplasmic expression, a versatile technology for the isolation of high-affinity antibodies from Escherichia coli-expressed libraries. Proc. Natl. Acad. Sci. USA 101, 9193-9198.   DOI   ScienceOn
6 Ivask, A., Hakkila, K., and Virta, M. 2001. Detection of organomercurials with sensor bacteria. Anal. Chem. 73, 5168-5171.   DOI   ScienceOn
7 Lee, J-S., Shin, K-S., Pan, J-G., and Kom, C-J. 2000. Surface-displayed viral antigens on Salmonella carrier vaccine. Nat. Biotechnol. 18, 645-648.   DOI   ScienceOn
8 Leonhartsberger, S., Huber, A., Lottspeich, F., and Bock, A. 2001. The hydH/G genes from Escherichia coli code for a 아연 and lead responsive two-component regulatory system. J. Mol. Biol. 307, 93-105.   DOI   ScienceOn
9 Mascher, T., Helmann, J.D., and Unden, G. 2006. Stimulus perception in bacterial signal-transducing histidine kinases. Microbiol. Mol. Biol. Rev. 70, 910-938.   DOI   ScienceOn
10 Munson, G.P., Lam, D.L., Outten, F.W., and O'Halloran, T.V. 2000. Identification of a copper-responsive two-component system on the chromosome of Escherichia coli K-12. J. Bacteriol. 182, 5864-5871.   DOI   ScienceOn
11 Ravikumar, S., Pham, V.D., Lee, S.H., Yoo, I.K., and Hong, S.H. 2012. Modification of CusSR bacterial two-component systems by the introduction of an inducible positive feedback loop. J. Ind. Microbiol. Biotechnol. 39, 861-868.   DOI   ScienceOn
12 Ravikumar, S., Yoo, I.K., Lee, S.Y., and Hong, S.H. 2011a. A study on the dynamics of the zraP gene expression profile and its application to the construction of zinc adsorption bacteria. Bioproc. Biosyst. Eng. 34, 1119-1126.   DOI   ScienceOn
13 avikumar, S., Yoo, I.K., Lee, S.Y., and Hong, S.H. 2011b. Construction of copper removing bacteria through the integration of two-component system and cell surface display. Appl. Biochem. Biotechnol. 165, 1674-1681.   DOI   ScienceOn
14 Sagi, E., Hever, N., Rosen, R., Bartolome, A.J., Premkumar, J.R., Ulber, R., Lev, O., Scheper, T., and Belkin, S. 2003. Fluorescence and bioluminescence reporter functions in genetically modified bacterial sensor strains. Sensor Actuat. B-Chem. 90, 2-8.   DOI   ScienceOn
15 Stock, A.M., Robinson, V.L., and Goudreau. P.N. 2000. Two-component signal transduction. Ann. Rev. Biochem. 69, 183-215.   DOI   ScienceOn
16 Yong, Y.C. and Zhong, J.J. 2009. A genetically engineered whole-cell pigment-based bacterial biosensing system for quantification of N-butyryl homoserine lactone quorum sensing signal. Biosen. Bioelectron. 25, 41-47.   DOI   ScienceOn
17 Taschner, S., Meinke, A., von Gabain, A., and Boyd, A.P. 2002. Selection of peptide entry motifs by bacterial surface display. Biochem. J. 367, 393-402.   DOI   ScienceOn