• 한국육종학회지
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• 제40권3호
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• pp.286-290
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• 2008

본 연구에서 분자육종을 위한 신속 DNA추출법을 개발하고자, 2% CTAB 추출법을 시스템적으로 개량한 Modified CTAB법에 대한 결과를 요약하면 아래와 같다. 1. DNA 추출용 샘플마쇄는 Voltex mixer, 3개 텅스텐 구슬 및 96 deep well block을 이용하였으며, 5분 이내에 96개 샘플을 분쇄할 수 있었다. 2. Modified CTAB DNA 추출법은 1.5 ml E-tube 대신에 1.2 ml 8연식 tube를 사용하였으며 DNA 추출, PCR작업 및 전기영동까지 일련의 작업이 8채널 멀티피펫을 이용할 수 있는 시스템적으로 개선하였다. 3. Modified CTAB법은 어린잎 뿐 만 아니라 이앙 후 30일째 채취한 잎에서도 DNA 추출이 가능하기 때문에 기존의 육종포장에 재식된 재료를 활용할 수 있는 장점이 있다.

• 한국강구조학회 논문집
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• 제15권2호
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• pp.167-174
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• 2003

본 연구는 보 플랜지와 스티프너의 용접부 양단, 기둥 모서리 부분 스티프너에 라운드를 두는 방법 등을 통하여 총 7개 시험체에 실물대 반복하중실험을 실시한 결과를 나타낸 것이다. 실험 결과를 통하여 다음과 같은 결론을 얻을 수 있었다. (1) 필레(fillet)를 둠으로써 응력집중을 줄여 내진성능을 크게 향상 시킬 수 있다. (2) 스티프너 폭의 증가로 접합부의 강성 및 강도는 증가하였지만 에너지 소산 능력은 감소하였다. (3) 모든 시험체는 규준에서 강진지역의 내진구조에 대해 요구하는 총 0.04 radian의 회전각을 발휘할 수 있는 것으로 나타났다.

• 한국윤활학회:학술대회논문집
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• 한국윤활학회 2002년도 proceedings of the second asia international conference on tribology
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• pp.363-364
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• 2002

Although the factors that cause the failure of orthopedic implants were not clearly determined, it was reported that the shapes of wear debris affect the tribological behavior of artificial implant. Many researches were conducted to examine the wear mechanism by debris but the role of debris shape in inflammatory reaction remains unclear. To observe the debris shape by addition of reinforcement, carbon nanotubes ( CNTs ) were added to ultra high molecular weight polyethylene ( UHMWPE ) to investigate the reinforcement effect of CNTs. CNTs which have a diameter of about 10-50 nm, while their length is about 3-5 nm were produced by the catalytic decomposition of the acetylene gas using a tube furnace. Plate on disc type wear test were performed to evaluate the tribological performance of UHMWPE composites reinforced with CNTs in lubricating condition ( bovine serum ). The wear losses of CNT added UHMWPE in bovine serum were significantly reduced. Worn surface and wear debris of UHMWPE with CNTs and without CNTs were compared to investigate the reinforcement effect of CNT on tribological behavior.

• 에너지공학
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• 제20권1호
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• pp.30-35
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• 2011

2중관형과 판형 열교환기에 에틸렌 클리콜-물 수용액으로 만들어진 아이스슬러리를 적용하는 경우의 유동 및 열적 특성을 규명하기 위하여 실험을 수행하였다. 아이스슬러리의 질량유속과 얼음 분율은 각각 800에서 3500 kg/$m^2s$과 0에서 25%이었다. 실험을 통해, 압력강하와 열전달율은 질량유속과 얼음 분율에 따라 증가하였다. 그러나 얼음 분율의 효과는 높은 질량유속 영역에서는 크지 않은 것으로 나타났다. 낮은 질량유속에서는 압력강하와 열전달율의 급속한 증가가 질량 유속에 관계되는 것으로 나타났다.

• Steel and Composite Structures
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• 제21권1호
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• pp.73-91
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• 2016

The use of box columns has been increased due to the rigidity in rigid orthogonal moment resisting frames. On the other hand, the installation and welding of necessary horizontal continuity plates inside the columns are both labor-consuming and costly tasks. Accordingly, in this paper, a new beam-to-box column connection by trapezoidal external stiffeners and horizontal bar mats is presented to provide seismic parameters. The proposed connection consists of eight external stiffeners in the level of beam flanges and five horizontal bar mats in Concrete Filled Tube (CFT) columns. The new connection effectively alleviates the stress concentration and moves the plastic hinge away from the column face by horizontal external stiffeners. In addition, the result shows that proposed connection has provided the required strength and rigidity of connection, so that the increased strength, 8.08% and rigidity, 3.01% are compared to connection with internal continuity plates, also the results indicate that this connection can offer appropriate ductility and energy dissipation capacity for its potential application in moment resisting frames in seismic region. As a result, the proposed connection can be a good alternative for connection with continuity plates.

• 설비공학논문집
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• 제22권4호
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• pp.248-257
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• 2010

An exhausted heat recovery system for a small gas engine cogeneration plant was investigated. The system was designed and built in a 300 kW class cogeneration demonstrative system. The basic performance was tested depending on load variation, and installed to a field site as a bottoming heat and power supply system. The exhaust gas heat exchangers (EGHXs) in shell-and-tube type and shell-and-plate type were tested. The entire efficiency of the cogeneration system was estimated between 85 to 90% under the 100% load condition, of which trend appears higher in summer due to the less thermal loss than in winter. Power generation efficiency and thermal efficiency was measured in a range of 31~33% and 54~57%, respectively.

• 한국기계가공학회지
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• 제14권6호
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• pp.7-13
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• 2015

The production processes were reviewed through the injection analysis of the shift lever as a core component of an auto lever installed in the automatic transmission of cars. The injection analysis was carried out for the shift lever and rod among the components in a shift lever module. The shift lever and rod are designed for injection molding with the insertion of a tube, a pin cable plate, and a steel rod for securing the strength of the product. The charging time, failure of injection molding, weld line, air trap, and deformation were reviewed according to this insert. Analyses on various gate positions were carried out for reviewing the cultivation and deformation of fiber around major components, such as the generation section of manipulation feeling and assembly section, so that optimal gate conditions might be reviewed and reflected in the mold design. Finally, we plan to compare the analysis results with the production of trial products.

• 한국유체기계학회 논문집
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• 제9권2호
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• pp.7-12
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• 2006

This study is to develop a system of electric power generation utilizing the wind resources available in the domestic wind environment. We tested drag-type vortical wind turbine models, which have two different types of blades: a flat plate and circular arc shape. Through a performance test, conditions of maximum rotational speed were found by measuring the rpm of wind turbine. The rotational speed was measured by a tachometer in a wind tunnel and the tunnel wind speed was by using a pilot-static tube and a micro manometer. The performance test for a prototype was accomplished by calculating power, power coefficient, torque coefficient from the measurement of torque and rpm by a dynamometer controller From the measurements for miniature turbine models with two different blades, the circular arc shape was found to Produce a maximum rotational speed for the same wind velocity condition. Based on this result, the prototype with the circular arc blade was made and tested. We found that it produces 500W at the wind velocity of 10.8 m/s and the power coefficient was 20%.

• 한국미생물·생명공학회지
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• 제20권1호
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• pp.1-5
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• 1992

유백피 추출물의 항세균 효과를 측정하기 위해 각각 다른 농도의 추출물의 함유한 배지에서 MIC를 측정하고, 증식에 미치는 영향을 조사한 결과는 다음과 같았다.이 추출물은 실험에서 사용된 세균 중 S.aureus, S.epidermidis, S.faecalis, B.subtilis, M.luteus와 같은 그람 양성균 및 E.coli, S.typhi, P.vulgaris, P.aeruginosa와 같은 그람 음성균에서 높았고, K.pneumoniae에 대해서는 항균력을 나타내지 않았다. 이 추출물 중의 항세균 효과를 가진 물질은 $121^{\circ}C$에서 20분간 멸균한 후에도 항세균 효과에는 별다른 변화가 없었다.

• Journal of Mechanical Science and Technology
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• 제14권11호
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• pp.1305-1318
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• 2000

The local wall shear stress in transitional boundary layer was estimated from the near-wall mean velocity data using the principle of Computational Preston Tube Method(CPM). The previous DNS and experimental databases of transitional boundary layers were used to demonstrate the accuracy of the method and to provide the applicable range of wall unit y(sup)+. The skin friction coefficients predicted by the CPM agreed well with those from previous studies. To reexamine the applicability of CPM, near-wall hot-wire measurement were conducted in developing transitional boundary layers on a flat plate with different freestream turbulence intensities. The intermittency profiles across the transitional boundary layers were reasonably obtained from the conditional sampling technique. An empirical correlation between the representative intermittency near the wall and free parameter K$_1$of the extended wall function of CPM has been newly proposed using the present and other experimental data. The CPM has been verified as a useful tool to measure the wall shear stress in transitional boundary layer with reasonable accuracy.