• Bulletin of the Korean Chemical Society
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• 제33권10호
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• pp.3248-3252
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• 2012

Vibrio extracellular metalloprotease (vEP), secreted from Vibrio vulnificus, shows various proteolytic function such as prothrombin activation and fibrinolytic activities. Premature form of vEP has an N-terminal (nPP) and a C-terminal (C-ter100) region. The nPP and C-ter100 regions are autocleaved for the matured metalloprotease activity. It has been proposed that two regions play a key role in regulating enzymatic activity of vEP. Especially, C-ter100 has a regulatory function on proteolytic activity of vEP. C-ter100 domain has been cloned into the E. coli expression vectors, pET32a and pGEX 4T-1 with TEV protease cleavage site and purified using gel-filtration chromatography followed by affinity chromatography. To understand how C-ter100 modulates proteolytic activity of vEP, structural studies were performed by heteronuclar multi-dimensional NMR spectroscopy. Backbone $^1H$, $^{15}N$ and $^{13}C$ resonances were assigned by data from standard triple resonance and HCCH-TOCSY experiments. The secondary structures of vEP C-ter100 were determined by TALOS+ and CSI software based on hydrogen/deuterium exchange. NMR data show that C-ter100 of vEP forms a ${\beta}$-barrel structure consisting of eight ${\beta}$-strands.

• BMB Reports
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• 제34권6호
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• pp.551-558
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• 2001

We extended the measurable time scale of DNA dynamics to submicrosecond using a long-lifetime metal-ligand complex, $[Ru(phen)_2(dppz)]^{2+}$ (phen=1,10-phenanthroline, dppz=dipyrido[3,2-a:2',3'-c]phenazine) (RuPD), which displays a mean lifetime near 350 ns. We partially characterized the fluorescence resonance energy transfer (FRET) in calf thymus DNA from RuPD to nile blue (NB) using frequency-domain fluorometry with a high-intensity, blue light-emitting diode (LED) as the modulated light source. There was a significant overlap of the emission spectrum of the donor RuPD with the absorption spectrum of the acceptor NB. The F$\ddot{o}$rster distance ($R_0$) that was calculated from the spectral overlap was $33.4\;{\AA}$. We observed dramatic decreases in the steady-state fluorescence intensities of RuPD when the NB concentration was increased. The intensity decays of RuPD were matched the closest by a triple exponential decay. The mean decay time of RuPD in the absence of the acceptor NB was 350.7 ns. In a concentration-dependent manner, RuPD showed rapid intensity decay times upon adding NB. The mean decay time decreased to 184.6 ns at $100\;{\mu}M$ NB. The FRET efficiency values that are calculated from the mean decay times increased from 0.107 at $20\;{\mu}M$ NB to 0.474 at $100\;{\mu}M$ NB concentration. The use of FRET with a long-lifetime metal-ligand complex donor is expected to offer the opportunity to increase the information about the structure and dynamics of nucleic acids.

• 대한자기공명의과학회:학술대회논문집
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• 대한자기공명의과학회 2002년도 제7차 학술대회 초록집
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• pp.134-134
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• 2002

목적： 심근경색의 가역성 여부 판단시 cardiac MR imaging(CMRI)와 myocardial SPECT의 정확성을 비교하기 위하여 대상 및 방법： 흉통을 주소로 내원한 환자 중 CMRI와 SPECT를 시행한 17를 대상으로 역행적으로 조사하였다. CMRI는 double and triple inversion recovery image(IR)에서 심벽의 고신호강도, 2D-FIESTA에서 심기능 분석을 조사하였다. 이어 gadopentetate를 주입하여 확산스캔에서 확산결손을 조사하였다. 연속하여 gadopentetate를 더 주입 후 5분 후 지연심근강조영상을 얻어 지연조영증강 유무와 미세혈류 폐쇄를 조사하였다. SPECT는 아데노신으로 stress 스캔을 얻은 후 T1-201을 재주사 후 rest 스캔하여 확산결손과 재분배를 조사하여 가역성을 판단하였다.

• 센서학회지
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• 제29권5호
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• pp.354-359
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• 2020

The high vacuum hermetic sealing technique ensures excellent performance of MEMS resonators. For the high vacuum hermetic sealing, the customization of anodic bonding equipment was conducted for the glass/Si/glass triple-stack anodic bonding process. Figure 1 presents the schematic of the MEMS resonator with triple-stack high-vacuum anodic bonding. The anodic bonding process for vacuum sealing was performed with the chamber pressure lower than 5 × 10^-6 mbar, the piston pressure of 5 kN, and the applied voltage was 1 kV. The process temperature during anodic bonding was 400 ℃. To maintain the vacuum condition of the glass cavity, a getter material, such as a titanium thin film, was deposited. The getter materials was active at the 400 ℃ during the anodic bonding process. To read out the electrical signals from the Si resonator, a vertical feed-through was applied by using through glass via (TGV) which is formed by sandblasting technique of cap glass wafer. The aluminum electrodes was conformally deposited on the via-hole structure of cap glass. The TGV process provides reliable electrical interconnection between Si resonator and aluminum electrodes on the cap glass without leakage or electrical disconnection through the TGV. The fabricated MEMS resonator with proposed vacuum packaging using three-layer anodic bonding process has resonance frequency and quality factor of about 16 kHz and more than 40,000, respectively.

• 한국항행학회논문지
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• 제25권6호
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• pp.536-542
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• 2021

본 논문에서는 WLAN(WiFi) 이중대역(2.4 ~ 2.484 / 5.15 ~ 5.825 GHz) 및 3.5 GHz 5G 대역(3.42~3.7 GHz) 을 위한 마이크로스트립 급전 구조를 가지는 굽은 가지 스트립을 가진 삼중대역 인쇄형 모노폴 안테나를 제안하였다. 3중 대역 안테나 구조로 수직으로 세워진 전형적인 λ/4 모노폴 스트립을 기반으로 하여 다중공진을 발생시키기 위한 굽은 스트립(strip)을 나무 가지(branch) 형태로 붙인 안테나 구조를 새롭게 제안하였다. 유전상수 4.3, 유전체 두께 1.6mm 의 FR4 기판에 28×40 mm²의 크기를 가진다. 측정 결과 2.4 GHz 대역에서 430 MHz (2.22~2.65 GHz), 3.5 GHz 5G 대역에서 450 MHz (3.38~3.83 GHz), 5 GHz 대역에서 2390 MHz (4.95~7.34 GHz)의 임피던스 대역폭을 나타냈다. 또한, 모든 대역에서 안정되고 무지향성 경향의 방사패턴을 얻을 수 있었으며, 각각 1.537 dBi, 1.878 dBi, 2.337 dBi의 이득을 가진다.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1996년도 춘계학술대회
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• pp.183-183
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• 1996

Bacteriophage T4 endonuclease V initiates the repair of ultraviolet (UV)-induced pyrimidine dimer photoproducts in duplex DNA. The mechanism of DNA strand cleavage involves four sequential stens: linear diffusion along dsDNA, pyrimidine dimer-specific binding,l pyrimidine dimer-DNA glycosylase activity, and Af lyase activity. Although crystal structure is known for this enzyme, solution structure has not been yet known. In order to elucidate the solution structure of this enzyme NMR spectroscopy was used. As a basis for the NMR peak assignment of the protein, HSQC spectrum was obtained on the uniformly $\^$15/N-labeled T4 endonuclease V. Each amide peak of the spectrum were classified according to amino acid spin systems by interpreting the spectrum of $\^$15/N amino acid-specific labeled T4 endonuclease V. The assignment was mainly obtained from three-dimensional NMR spectra such as 3D NOESY-HMQC, 3D TOCSY-HMQC. These experiments were carried out will uniformly $\^$15/N-labeled sample. In order to assign tile resonance of backbon atom, triple-resonance theree-dimensional NMR experiments were also performed using double labeled($\^$15/N$\^$13/C) sample. 3D HNCA, HN(CO)CA, HNCO, HN(CA)HA spectra were recorded for this purpose. The results of assignments were used to interpret the interaction of this enzyme with DNA. HSQC spectrum was obtained for T4 endonuclease V with specific $\^$15/N-labeled amino acids that have been known for important residue in catalysis. By comparing the spectrum of enzyme*DNA complex with that of the enzyme, we could confirm the important role of some residues of Thr, Arg, Tyr in activity. The results of assignments were also used to predict the secondary structure by chemical shift index (CSI).

• 대한자기공명의과학회:학술대회논문집
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• 대한자기공명의과학회 2001년도 제6차 학술대회 초록집
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• pp.140-140
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• 2001

We present two cases of surgically proven focal nodular hyperplasia whou underwent tri contrast-enhance MR imaging using gadolinium chelates, mangafodipir trisodium, and ferumoxides After the unehanced MR images were obtained, dynamic gadolinium-enhanced T1-weighted imagi were performed, then mangafodipir enhanced and ferumoxides-enhanced images were obtained. In one case, the mass was isointense on both T1- and T2-weighted images on the unehanced M images, iso and slightly hyperintense on ferumoxides-enhanced FSE and GRE images, strong hyperintense on the mangafodipir enhanced and gadolinium enhanced arterial phase images. In th other case, the mass was isointense on T2-weighted and hypointense on T1-weighted image isointense on ferumoxides-enhanced images, and hyperintense on mangafodipir enhanced an gadolinium enhanced arterial phase images. Triple contrast enhanced MR images were useful correctly diagnose these two cases preoperatively.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1995년도 춘계학술대회
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• pp.80-80
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• 1995

T4 Endonuclease V (Mw 16,000) acts as a repair enzyme for UV induced pyrimidine dimers in DNA. Many researchers have studied the biochemical characteristics of the enzyme. However the precise action mechanism of T4 endo V has not fully elucidated yet. In our laboratory NMR spectroscopy technique is being used for the structural study of T4 endo V. Because of its low temperature stability and high content of ${\alpha}$-helix, the conventional $^1$H NMR technique was inapplicable. Therefore we utilized stable isotope labeling technique and so far prepared about 10 amino acid specific labeled proteins. The HSQC spectra of amino acid specific labeled proteins will help us to interpret the triple resonance 3D, 4D data which are under processing, We also studied the behaviors of specific amino acid residues whose roles might be critical. When the enzyme labeled by $\^$15/N-Thr was mixed with the substrate oligonucleotide (semispecific -TT- sequence), one crosspeak in its HSQC spectrum was completely desappeared, which means that one of seven Thr residues is in the binding site of the enzyme with DNA, This result is well consistent with previous report that implicated the Thr 2 residue in the activity of the enzyme. Similar studies were carried on the behaviors of Arg and Tyr residues.

• Bulletin of the Korean Chemical Society
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• 제30권1호
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• pp.149-152
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• 2009

Hydrotalcites are anionic clays that are quite prevalent in nature and their importance is growing more and more because of their very wide range of potential applications and uses. Understanding the structural and compositional changes that occur on the molecular scale during the thermal decomposition of hydrotalcite compounds is essential for the basic prediction and comprehensive understanding of the behavior and technical application of these materials. In this study, several hydrotalcite compounds calcined at different temperatures for applications in a chlorine resistant textile were prepared and 27-Aluminm solid-state nuclear magnetic resonance (NMR) spectroscopy was used as a tool to study their local structure and behavior. The changes in the Al coordination of the hydrotalcite compounds were investigated with one dimensional (1D) solid-state magic angle spinning (MAS) NMR spectroscopy. The two broad resonances arising from the structurally different Al coordinations of these compounds were clearly resolved by two dimensional (2D) triple quantum magic angle spinning (3QMAS) NMR spectroscopy.

• 대한기계학회논문집B
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• 제39권7호
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• pp.579-589
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• 2015

본 연구의 목적은 수직으로 강제 진동하는 소수성 표면 위에 놓인 액적의 유동 및 증발 특성을 이해하는 것이다. 액적의 공진주파수를 예측하기 위해서 Lamb과 Strani and Sabetta의 이론적 고유진동수식을 이용하였고, 실험값과 비교하여 보다 근접한 고유진동수 식에 대해 타당성을 검증하였다. 액적의 형상 및 내부 유동을 가시화하기 위해 초고속카메라, 초접사렌즈 그리고 연속광을 사용하여 진동하는 소수성 표면 위 액적의 유동 및 증발 특성을 확인하였다. 그 결과 각각의 모드에서 액적은 다양한 형상을 가졌으며, 각각의 액적 내부에서 복잡한 와류가 관찰되었다. 일반적으로, 유동흐름이 대칭축을 따라 위로 상승하여 액적상단에서 표면을 따라 접촉선부근으로 이동하였고, 2차, 4차 모드는 분기형, 6차, 8차 모드는 큰 타원형의 유동패턴을 갖는 것을 확인하였다. 여러 가지 모드 중 4차 모드에서 가장 빠른 유동속도를 가졌으며, 다음은 8, 6, 2차 모드 순서였다. 네 가지 진동 모드에서의 증발률은 4, 8, 6, 2차 모드 순서로 빨랐으며, 각각의 공진에서는 그 주위 주파수 영역보다 빠른 증발률을 보였다. 마지막으로 진동을 이용한 액적의 증발은 4차 모드에서 진행되어야 보다 효율적인 진동 증발을 유도할 수 있다.