• Korean journal of applied entomology
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• v.50 no.3
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• pp.253-256
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• 2011

The walnut leaf beetle, Gastrolina depressa, belongs to family Chrysomelidae (Coleoptera), and is one of the major pests of walnut trees. G. depressa eggs were oval and dark orange. The three G. depressa larval instars were gray in color. The larval period was approx. 8.14 days ($24^{\circ}C$, 16L:8D). Adults of both sexes were dark blue, and females were larger than males. Overwintered adults emerged in late April, and laid eggs in clusters on young leaves of the host tree. Mature 3rd instar larvae pupated after a short prepupal period. Adults emerged starting mid-May and entered into diapause shortly afterward.

• Journal of the Korean Institute of Landscape Architecture
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• v.18 no.3 s.39
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• pp.1-9
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• 1990

Landscape plants as attenuator of vehicular noise were basically experimented with 8 kinds of trees and shrubs at the university nursery, and checked the attenuating effects by the planted distances, species and sound frequencies. The experimental results are shown and sumarized as follows ; 1. Most plants showed the considerable noise attenuating function in the sound frequencies of 63Hz and 1,000Hz. Tall trees such as Juniperus chinensis var. kaizuka and Magnolia grandiflora showed better effects than shrubs or small trees such as Gardenia jasminoides, Osmanthus fragnance, Osmanthrs heterophyllus, Rhododendron indicum, Chamaecyparis obtusa 'Tetragona Aurea' and Pinus paruiflora in 63Hz frequency, but shrubs showed better effects in 1,000Hz. 2. Trees showed 6~8dB attenuation and shrubs 4~5dB regardless an increase of distance between sound source and receiver in 63Hz. 10 meter width of tree belt could reduce 10 more dB, but 5 meter of shrub belt showed the same effect in 1,000Hz. 3. Trees having more leaves and wider crown showed constantly increase of attenuation of sound by the distance in 1,000Hz, and distinct increase of attenuation were observed in 2,000 more Hz.

• Journal of Ecology and Environment
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• v.29 no.1
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• pp.41-47
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• 2006

We investigated the enect of $O_3$ on the photosynthetic characteristics of tree of heaven (Ailanthus altissima) that is naturalized plant and used as restoration plant for contaminated area. Two-year-old seedlings were planted to pots and transferred into closed $O_3$ chamber. Photosynthetic pigments contents and photosynthetic characteristics were measured every three weeks under 100 pub $O_3$ fumigation. There was no visible foliar injury by $O_3$ exposure and contents of photosynthetic pigments did not show significant differences between control and $O_3$-treated seedlings. Also there were no significant differences in stomatal conductance, and water use efficiency. But photosynthetic rate and apparent quantum yield (AQY) of $O_3$ treated seedlings were reduced after nine weeks of ozone fumigation. In addition, the reduction of carboxylation efficiency and photorespiration were observed in the leave of $O_3$ treated seedlings after six weeks. In accordance with our result, carbon fixation system of A. altissima was most sensitive to $O_3$ stress to evaluate physiological damage induced by $O_3$.

• Journal of Plant Biotechnology
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• v.5 no.2
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• pp.131-135
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• 2003

Callus and cell suspension cultures of Eurycoma longifolia Jack were initiated from leaves of different trees. The leaf explant of tree Eu9 produced the most calli and also induced high cell biomass in the cell suspension culture. Optimum production of cell biomass could be initiated in proliferating culture medium with a pH of 5.75 prior to autoclaving. The effects of macronutrient inorganic salts of Murashige and Skoog (MS) liquid medium supplemented with X on production of cell biomass of Eurycoma longifolia were also investigated. The highest cell biomass was produced in MS medium containing macronutrients of $21\;mM\;NH_4NO_3,\;12.25\;mM\;KNO_3,\;3.00\;mM\;CaCl_2.2H_2O,\;0.575\;mM\;MgSO_4.7H_2O$, and $1.83\;mM\;KH_2PO_4$. A new medium labeled as TAM was formulated for the production of Eurycoma longifolia cell biomass in the cell suspension culture.

• Korean Journal of Plant Resources
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• v.32 no.4
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• pp.275-281
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• 2019

The pathological condition of excessive melanogenesis causing freckles, melasma, senile lentigo, pigmented acne scars, and cancer has a critical impact on the wellness of individuals. The mechanism of melanogenesis is related to the expression of melanogenic enzymes. Here, we evaluated the inhibitory effect of pine cone (Pinus densiflora) extracts on melanogenesis. P. densiflora, the Korean Red Pine, is the predominant tree species in the cool, temperate forests of northeast Asia, occurring in pure stands across Korea, Japan, and parts of northern China and Russia. P. densiflora leaves, pollen, and bark have been widely used for traditional medicine, or edible purposes. However, pine cones are rarely used as natural raw materials, although they contain many bioactive phytochemicals. The pine cone ethyl acetate fraction (PEF) showed no toxicity to B16F10 cells at a concentration of less than $100{\mu}g/mL$. PEF inhibited the expression of microphthalmiaassociated transcription factor (MITF), tyrosinase and tyrosinase-related factors in B16F10 cells treated with 3-Isobutyl1-methylxanthine (IBMX). These results suggest that pine cones can be used as an effective natural melanogenesis inhibitory agent.

• Journal of Soil and Groundwater Environment
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• v.26 no.6
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• pp.82-94
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• 2021

Facilities for low impact development (LID) or groundwater recharge have the high potential spreading groundwater nitrate contamination because of the rapid infiltration. This study was initiated to remove nitrate from the waters using agricultural byproducts as organic sources for denitrification during infiltration. As the first step of this purpose, we experimentally tested the denitrifying efficiency of 4 organic materials (pine tree woodchips, cherry leaves, rice straws, and rice hulls) and tried to identify the key factors controlling the efficiency. For this study, we precisely investigated the change of chemical reactions during the experiment by analyzing various geochemical parameters. The result shows that the denitrification efficiency is not simply linked to the availability of the easily decomposable contents in the organic matter. It is found that avoiding the severe pH decrease due to the CO₂ generation is the essence to derive the efficient denitrifying conditions when organic matters were used.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.119-119
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• 2019

Pinus densiflora, the Korean Red Pine, is the predominant tree species of the cool, temperate forests of northeast Asia, occurring in pure stands across Korea, Japan, and parts of northern China and Russia. Pinus densiflora leaves, pollen, and bark have been widely used for traditional medicine, or edible purposes. However, pine cones contain many bioactive phytochemicals, but they are rarely used as natural raw materials. This study was conducted to evaluated the anti-inflammatory effect of pine cone extracts and its possbility of natural sources were evaluated. Pine cones were extracted with 80% methanol, concentrated and then partitioned with ethyl acetate, and the organic layer was used as a sample. The Pine cone Ethyl acetate Fraction (PEF) showed no toxicity to RAW 264.7 cells at a concentration of less than $50{\mu}g/ml$. PEF inhibited the production of nitric oxide (NO) in RAW 264.7 cells treated with lipopolysaccharide (LPS). Also, It suppressed the expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and transcription of nuclear factor-kappa B (NF-${\kappa}B$). These results suggest that pine cones can be used as an effective natural material for anti-inflammatory agent.

• Biomedical Science Letters
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• v.28 no.2
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• pp.92-100
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• 2022

Ginkgo biloba Leaf Extract (GBE) is an extract from leaves of the Ginkgo biloba tree, widely used as a health supplement. GBE can inhibit the proliferation of several types of tumor cell. Although it is known to have anti-cancer effects in breast cancer and skin cancer, research related to gastric cancer is still insufficient. Based on results showing anti-cancer effects on solid cancer, we aimed to determine whether GBE has similar effects on gastric cancer. In this study, the anti-cancer effect of GBE in gastric adenocarcinoma was investigated by confirming the cell proliferation inhibitory effect of AGS cells. We also evaluated whether GBE regulates expression of the tumor suppressor protein p53 and Rb. GBE has apoptotic effects on AGS cells that were confirmed by changes in anti-apoptosis protein Bcl-2, Bcl-xl and pro-apoptosis protein Bax levels. Wound healing and cell migration were also decreased by treatment with GBE. Furthermore, we verified the effects of GBE on mitogenic signaling by investigating AKT target gene expression levels and revealed downregulated Sod2 and Bcl6 expression. We also confirmed that expression of inflammation-related genes decreased in a time-dependent manner. These results indicate that GBE has an anti-cancer effect on human gastric cancer cell lines. Further research on the mechanism of the anti-cancer effect will serve as basic data for possible anti-cancer drug development.

• Mycobiology
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• v.50 no.5
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• pp.302-316
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• 2022

Many Apiospora species have been isolated from bamboo plants - to date, 34 bambusicolous Apiospora species have been recorded. They are known as saprophytes, endophytes, and plant pathogens. In this study, 242 bambusicolous Apiospora were isolated from various bamboo materials (branches, culms, leaves, roots, and shoots) and examined using DNA sequence similarity based on the internal transcribed spacer, 28S large subunit ribosomal RNA gene, translation elongation factor 1-alpha, and beta-tubulin regions. Nine Apiospora species (Ap. arundinis, Ap. camelliae-sinensis, Ap. hysterina, Ap. lageniformis sp. nov., Ap. paraphaeosperma, Ap. pseudohyphopodii sp. nov., Ap. rasikravindrae, Ap. saccharicola, and Ap. sargassi) were identified via molecular analysis. Moreover, the highest diversity of Apiospora was found in culms, and the most abundant species was Ap. arundinis. Among the nine Apiospora species, two (Ap. hysterina and Ap. paraphaeosperma) were unrecorded in Korea, and the other two species (Ap. lageniformis sp. nov. and Ap. pseudohyphopodii sp. nov.) were potentially novel species. Here, we describe the diversity of bambusicolous Apiospora species in bamboo organs, construct a multi-locus phylogenetic tree, and delineate morphological features of new bambusicolous Apiospora in Korea.

• Mycobiology
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• v.51 no.2
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• pp.67-71
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• 2023

A powdery mildew (Erysiphaceae) has been continuously collected on the leaves of Lonicera harae in the southern part of the Korean Peninsula, where this shrub is indigenous. Microscopic examination of the asexual morphs revealed that the current collections are differentiated from the all known Erysiphe species on Lonicera spp. by its longer conidiophores and longer conidia. Although the morphology of the chasmothecia is reminiscent of Erysiphe ehrenbergii and E. lonicerae, the specimens on L. harae differ from them in having smaller ascospores. A phylogenetic tree generated from a combined dataset of the internal transcribed spacer region and 28S rDNA gene sequences demonstrates that sequences obtained from three powdery mildew collections on L. harae clustered together as an independent species clade with high bootstrap values distant from other Erysiphe species on Lonicera, representing a species of its own. Based on morphological differences and molecular-phylogenetic results, the powdery mildew on L. harae is proposed as a new species, Erysiphe lonicerigena, and the holomorph of the fungus is described and illustrated in this study.