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http://dx.doi.org/10.7732/kjpr.2019.32.4.275

Inhibitory Effects of Pine Cone (Pinus densiflora) on Melanogenesis in B16F10 Melanoma Cells  

Lee, Seung-Hyun (Industry-Academia Cooperation Foundation, Jungwon University)
Jang, Tae-Won (Department of Medicinal Plant Resources, Andong National University)
Choi, Ji-Soo (Department of Medicinal Plant Science, Jungwon University)
Mun, Jeong-Yun (Department of Pharmaceutical Science, Jungwon University)
Park, Jae-Ho (Department of Pharmaceutical Science, Jungwon University)
Publication Information
Korean Journal of Plant Resources / v.32, no.4, 2019 , pp. 275-281 More about this Journal
Abstract
The pathological condition of excessive melanogenesis causing freckles, melasma, senile lentigo, pigmented acne scars, and cancer has a critical impact on the wellness of individuals. The mechanism of melanogenesis is related to the expression of melanogenic enzymes. Here, we evaluated the inhibitory effect of pine cone (Pinus densiflora) extracts on melanogenesis. P. densiflora, the Korean Red Pine, is the predominant tree species in the cool, temperate forests of northeast Asia, occurring in pure stands across Korea, Japan, and parts of northern China and Russia. P. densiflora leaves, pollen, and bark have been widely used for traditional medicine, or edible purposes. However, pine cones are rarely used as natural raw materials, although they contain many bioactive phytochemicals. The pine cone ethyl acetate fraction (PEF) showed no toxicity to B16F10 cells at a concentration of less than $100{\mu}g/mL$. PEF inhibited the expression of microphthalmiaassociated transcription factor (MITF), tyrosinase and tyrosinase-related factors in B16F10 cells treated with 3-Isobutyl1-methylxanthine (IBMX). These results suggest that pine cones can be used as an effective natural melanogenesis inhibitory agent.
Keywords
B16F10; Melanogenesis; Pine cone; Pinus densiflora;
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