• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.7 no.3
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• pp.79-84
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• 1998

It is very difficult to analyze the transient temperature distribution during quenching of the steel because of coupled effects among temperature, structures and stresses. In this paper, using Inoue's equation of evolution and mixture rule, transient temperature distribution is calculated by the finite element method considering latent heat of transformation structure and temperature dependence of physical and mechanical prperties for the 0.45% carbon cylindrical steel bar with 40mm diameter and 20mm height during end-quenching.

• Molecules and Cells
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• v.39 no.9
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• pp.705-713
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• 2016

The efficiency of Agrobacterium-mediated transformation in plants depends on the virulence of Agrobacterium strains, the plant tissue culture conditions, and the susceptibility of host plants. Understanding the molecular interactions between Agrobacterium and host plant cells is crucial when manipulating the susceptibility of recalcitrant crop plants and protecting orchard trees from crown gall disease. It was discovered that Arabidopsis voltage-dependent anion channel 1 (atvdac1) mutant has drastic effects on Agrobacterium-mediated tumorigenesis and growth developmental phenotypes, and that these effects are dependent on a Ws-0 genetic background. Genetic complementation of Arabidopsis vdac1 mutants and yeast porin1-deficient strain with members of the AtVDAC gene family revealed that AtVDAC1 is required for Agrobacterium-mediated transformation, and there is weak functional redundancy between AtVDAC1 and AtVDAC3, which is independent of porin activity. Furthermore, atvdac1 mutants were deficient in transient and stable transformation by Agrobacterium, suggesting that AtVDAC1 is involved in the early stages of Agrobacterium infection prior to transferred-DNA (T-DNA) integration. Transgenic plants overexpressing AtVDAC1 not only complemented the phenotypes of the atvdac1 mutant, but also showed high efficiency of transient T-DNA gene expression; however, the efficiency of stable transformation was not affected. Moreover, the effect of phytohormone treatment on competence to Agrobacterium was compromised in atvdac1 mutants. These data indicate that AtVDAC1 regulates the competence of Arabidopsis to Agrobacterium infection.

• JSTS:Journal of Semiconductor Technology and Science
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• v.17 no.2
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• pp.288-293
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• 2017

Transient simulation capability with an implicit time derivation method is a missing feature in deterministic Boltzmann equation solvers. The H-transformation, which is critical for the stable simulation of nanoscale devices, introduces difficulties for the transient simulation. In this work, the transient simulation of graphene sheets is reported. It is shown that simulation of homogeneous systems can be done without abandoning the H-transformation, as much as a specially designed discretization method is employed. The AC mobility and step response of the graphene sheet on the $SiO_2$ substrate are simulated.

• Journal of Plant Biotechnology
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• v.31 no.3
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• pp.185-190
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• 2004

We established a transient gene expression system in chili pepper leaves based on Agrobacterium-mediated transformation of GUS gene. For the best GUS transient expression, two step culture system was adopted. When the Agrobacterium tumefaciens cell density of pre-culture was $A_{600nm}$ 0.3, the cells were harvested and diluted to $A_{600nm}$ 0.8 with virulence induction medium after cell harvested. The addition of acetosyringone (200 $\mu$M) in virulence induction step was a key factor for successful transient expression. Additionally, Younger leaves showed more effective transient expression than older leaves. Temporally, the strongest intensity of GUS expression was detected at 2 days after infiltration. These results demonstrate that Agrobacterium-mediated transient expression can be used for a simple in vivo assays of plant promoters, transcription factors and furthermore provide efficient protocol for chili pepper transformation.

• Journal of Plant Biotechnology
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• v.7 no.2
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• pp.113-121
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• 2005

The objective of this study was to identify the major parameters controlling DNA delivery by particle bombardment to immature embryos of Chinese spring wheat (Triticum aestivum L.). Efficiency of DNA (uidA gene) delivery was assessed by transient GUS (${\beta}$-glucuronidase) expression in bombarded tissues. Of the parameters analyzed, acceleration pressure, bombardment distance, chamber vacuum pressure, bombardment times, osmotic conditioning of culture had a remarkable influence on transient gene expression. A bombardment procedure suitable for Chinese spring wheat cultivars was developed which allowed high-efficiency DNA delivery combined with reduced damage to target tissues. The high efficiency made the system practical for wheat genetic transformation research and accelerating wheat breeding programs.

• Journal of Plant Biotechnology
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• v.7 no.4
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• pp.219-223
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• 2005

We investigated chemical-wounding effect on Agrobacterium-mediated Chinese cabbage transformation via vacuum infiltration. Pre-germinated or germinating Chinese cabbage seeds were infiltrated with Agrobacterium tumefaciens LBA4404 cells carrying either GUS gene (pBI121) or hepatitis B virus surface antigen DNA (pBIHBsAg). Prior to agroinfiltration process, the seeds were soaked in sodium hydrosulfite (SHS) solution or just in sterile water as a control. Comparative transformation efficiency was determined by both of histochemistry and ELISA. We could demonstrate that SHS solution treatment especially to 1-day or 2-days old germinating seeds efficiently improved transformation process, and therefore, transient expression level. This strongly indicated that Agrobacterium infection could be facilitated indeed by SHS-causing wounds on Chinese cabbage seeds.

• Journal of Power Electronics
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• v.9 no.3
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• pp.507-515
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• 2009

This paper proposes an autonomous decentralized control for a parallel connected uninterruptible power supply (UPS) system based on a fast power detection method using a FPGA based hardware controller for a single phase system. Each UPS unit detects only its output voltage and current without communications signal exchange and a quasi dq transformation method is applied to detect the phase and amplitude of the output voltage and the output current for the single phase system. Fast power detection can be achieved based on a quasi dq transformation, which results in a realization of very fast transient response under rapid load change. In the proposed method, the entire control system is implemented in one FPGA chip. Complicated calculations are assigned to hardware calculation logic, and the parallel processing circuit makes it possible to realize minimized calculation time. Also, an Nios II CPU core is implemented in the same FPGA chip, and the software can be applied for non-time critical calculations. Applying this control system, an autonomous decentralized UPS system with very fast transient response is realized. Feasibility and stable operation are confirmed by means of an experimental setup with three UPSs connected in parallel. Also, rapid load change is applied and excellent performance of the system is confirmed in terms of transient response and stability.

• Journal of Applied Biological Chemistry
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• v.48 no.4
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• pp.229-230
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• 2005

• KSBB Journal
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• v.21 no.6 s.101
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• pp.489-492
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• 2006

For molecular breeding purpose, genetic transformation of Brassica napus cultivars has been extensively performed using Agrobacterium method. B. napus cv. napus, one of major oil crops, can be transformed via Agrobacterium-based method. We demonstrated that Agrobacterium-mediated transformation via vacuum infiltration slightly worked for the seedlings of B. napus cv. napus according to fluorometric GUS enzyme analysis. In contrast, transformation efficiency was highly enhanced when the seedlings, prior to agroinfiltration, were treated with sodium hydrosulfite solution as a chemical wounding agent. GUS gene expression in transformed seedlings that was confirmed by RT-PCR suggests their usefulness for the development of transient expression system.

• Advances in materials Research
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• v.6 no.1
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• pp.27-43
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• 2017

The transient thermo-mechanical behavior of a simply-supported beam made of a functionally graded material (FGM) under the effect of a moving heat source is investigated. The FGM consists of a ceramic part (on the top), which is the hot side of the beam as the heat source motion takes place along this side, and a metal part (in the bottom), which is considered the cold side. Grading is in the transverse direction, with the properties being temperature-dependent. The main steps of the thermo-elastic modeling included deriving the partial differential equations for the temperatures and deflections in time and space, transforming them into ordinary differential equations using Laplace transformation, and finally using the inverse Laplace transformation to find the solutions. The effects of different parameters on the thermo-mechanical behavior of the beam are investigated, such as the convection coefficient and the heat source intensity and speed. The results show that temperatures, and hence the deflections and stresses increase with less heat convection from the beam surface, higher heat source intensity and low speeds.