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Effect of Sodium Hydrosulfite Solution on Agrobacterium-Mediated Chinese Cabbage Transformation and Transient Expression  

Park Hee-Sung (Department of Biotechnology, Catholic University of Daegu)
Shin Dong-Il (Department of Biotechnology, Catholic University of Daegu)
Publication Information
Journal of Plant Biotechnology / v.7, no.4, 2005 , pp. 219-223 More about this Journal
Abstract
We investigated chemical-wounding effect on Agrobacterium-mediated Chinese cabbage transformation via vacuum infiltration. Pre-germinated or germinating Chinese cabbage seeds were infiltrated with Agrobacterium tumefaciens LBA4404 cells carrying either GUS gene (pBI121) or hepatitis B virus surface antigen DNA (pBIHBsAg). Prior to agroinfiltration process, the seeds were soaked in sodium hydrosulfite (SHS) solution or just in sterile water as a control. Comparative transformation efficiency was determined by both of histochemistry and ELISA. We could demonstrate that SHS solution treatment especially to 1-day or 2-days old germinating seeds efficiently improved transformation process, and therefore, transient expression level. This strongly indicated that Agrobacterium infection could be facilitated indeed by SHS-causing wounds on Chinese cabbage seeds.
Keywords
Sodium hydrosulfite; Chemical wounding; Agroinfiltration; Transformation efficiency;
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1 Schaart JG, Salentijn EMJ, Krens FA (2002) Tissue-specific expression of the $\beta$ -glucuronidase reporter gene in transgenic strawberry (Fragaria x ananassa) plants. Plant Cell Rep 21: 313-319   DOI   ScienceOn
2 Vitha S, Benes K, Phillips JP, Gartland KMA (1995) Histochemical GUS analysis. In: Gartland KMA, Davey MR (eds), Agrobacterium Protocols, Humana Press, pp 185-193
3 Fisher R, Vaquero-Martin C, Sack M, Drossard J, Emans N, Commandeur U (1999) Towards molecular farming in the future: transient protein expression in plants. Biotechnol Appl Biochem 30: 113-116
4 Joung YH, Youm JW, Jeon JH, Lee BC, Ryu CJ, Hong HJ, Kim HC, Joung H, Kim HS (2004) Expression of the hepatitis B surface Sand preS2 antigens in tubers of Solanum tuberosum. Plant Cell Rep. 22: 925-930
5 Lohrke SM, Yang H, Jin S (2001) Reconstitution of acetosyringonemediated Agrobaderium tumefaciens virulence gene expression in the heterologous host Escherichia coli. J Bacteriol 183: 3704-3771   DOI   ScienceOn
6 Bechtold N, Ellis J, Pelletier G (1993) In planta Agrobacterium mediated gene ransfer by infiltration of adult Arabidopsis thaliana plants. CR Acad Sci Paris 316: 1194-1199
7 Fisher R, Hoffmann K, Schillberg, Emans N (2000) Antibody production by molecular farming in plants. J biol Regul Homeost Agents 14: 83-92
8 Sunil Kumar GB, Ganapathi TR, Revathi CJ, Prasad KSN Bapat VA (2003) Expression of hepatitis B virus surface antigen in tobacco cell suspension cultures. Protein Expr Purif 32: 10-17   DOI   ScienceOn
9 Doran PM (2000) Foreign protein production in plant tissue cultures. Curr Opin Biotech 11: 199-204   DOI   PUBMED   ScienceOn
10 Jefferson RA, Kavanagh TA, Bevan, MW (1987) GUS fusions: $\beta$ -glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 6: 3901-3907
11 Cho HS, Cao J, Ren JP, Earle ED (2001) Control of lepidopteran insect pests in transgenic Chinese cabbage transformed with synthetic Bacillus thuringiensis cry1C gene. Plant Cell Rep 20: 1-7   DOI   ScienceOn
12 Wagner B, Fuchs H, Adhami F, Ma Y, Scheiner O, Breiteneder H (2004) Plant virus expression systems for transient production of recombinant allergens in Nicotiana benthamiana. Methods 32: 227-234   DOI   ScienceOn
13 Giddings G (2001) Transgenic plants as protein factories. Curr Opin Biotech 12: 450-454   DOI   PUBMED   ScienceOn
14 Joh LD, Wroblewski T, Ewing NN, VanderGheynst JS (2005) High-level transient expression of recombinant protein in lettuce. Biotechnol Bioeng 30: 861-871
15 Cho YN, Park SY, Noh TK, Song MJ, Park YS, Min BW (2003) Transformation of Chinese cabbage with L-gluno-$\gamma$-lactone-oxidase(GLOase)-encoding gene using Agrobacterium tumefaciens. Kor J Hort Sci 21: 9-13
16 Jefferson RA (1987) Assaying chimeric genes in plants: the gus gene fusion system. Plant Mol Biol Rep 5: 387-405   DOI
17 Sojikul P, Buehner N. Mason HS (2003) A plant signal peptidehepatitis B surface antigen fusion protein with enhanced stability and immunogenecity expressed in plant cells. Proc Natl Acad Sci USA 100: 2209-2214   DOI   ScienceOn
18 Christey MC, Sinclair BK, Braun RH, Wyke L (1997) Regeneration of transgenic vegetable Brassicas (Brassica oleracea and B. campestris) via Ri-mediated transformation. Plant Cell Rep 16: 587-593   DOI   ScienceOn