• 미생물학회지
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• 제36권2호
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• pp.109-111
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• 2000

Lactobacillus casei ssp. casei NCIB 4114 균주로부터 3,5kb의 플라스미드를 분리하여, 이 플라스미드를 함유하는 Escherichia coli-Lactobacillus 셔틀 백터(shuttle vector)들을 만들었다. tu틀 벡터들은 모두 electroporation에 의해 성공적으로 형질전환 되었다. Electroporation의 최적조건은 벡터DNA 1$\mu$g당 $2{\times}10^5$ 형질전환체의 효율이었고, 이들 벡터의 성공적 도입은 이들 벡터의 유산균에서의 음식등급 벡터로의 사용 가능성을 제시하였다.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2001년도 추계종합학술대회
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• pp.579-582
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• 2001

벡터변형은 벡터 양자화(VQ)와 부호화를 통합한 새로운 방법이다. 최근까지 부호화에 적용된 코드북 생성은 LBG 알고리즘이었으나 신경회로망을 기반으로 한 자기생성 특성맵(SOFM: Self Organizing Feature Map)의 장점을 이용하면 시스템의 성능을 개선할 수 있다는 점에 착안하였다. 본 논문에서는 SOFM 알고리즘을 적용한 VTC(Vector Transformation coding)코드북 생성과 LBG 알고리즘의 부호화률에 대한 결과를 비교하여 분석하였다. 벡터 양자화의 문제점은 계산의 복잡성과 코드북 생성에 있으므로 본 연구에서는 이 문제의 해결을 위해 신경망 접근법을 제안한다.

• 한국식물병리학회지
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• 제11권1호
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• pp.66-72
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• 1995

The coat protein (CP) gene of cucumber mosaic virus-As (CMV-As) strain was engineered for expression in the plant by using the cauliflower mosaic virus 35S transcript regulatory sequences. The CP gene was cloned into an Agrobacterium-derived binary vector. A chimeric gene was constructed by the cDNA of CMV-As CP and plant expression vector pBI121. The clone, pCMAS66, was first introduced into the phagemid vector pSPORT1 for situating sense orientation for translation and making restriction sites in order to re-introduce plant expression vector, pHI121. The resulting subclone pCASCP02 and plant expression vector pBI121 were treated with BamHI-SacI for excising the target gene and removing GUS gene, respectively. After Agrobacterium transformation by freeze-thaw technique, the clone, pCMASCP121-123 which contains sense orientation of the target gene, was selected and confirmed by restriction endonuclease analysis. The CMV-As CP gene was introduced into A. tumefaciens. The results on tobacco plant transformation with the vector system revealed that the system could be successfully introduced and showed high frequency of selection to putative transformations.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1997년도 하계학술대회 논문집 B
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• pp.422-424
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• 1997

In this paper, we characterize the whole class of vector fields that can be linearized by a given nominal state transformation and a feedback linearizing controller. The necessary and sufficient condition for a given uncertain vector field to be so-called "completely linearizable by the nominal coordinate transformation" is given in terms of Lie Bracket of uncertain vector fields and some suitable vector fields of the nominal system.

• Journal of Plant Biotechnology
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• 제46권4호
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• pp.323-330
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• 2019

The high expression level of industrial and metabolically important proteins in plants can be achieved by plastid transformation. The CaIA vector, a Capsicum-specific vector harboring aadA (spectinomycin resistance), is a selectable marker controlled by the PsbA promoter, and the terminator is flanked by the trnA and trnI regions of the inverted repeat (IR) region of the plastid. The CaIA vector can introduce foreign genes into the IR region of the plastid genome. The biolistic method was used for chloroplast transformation in Scoparia dulcis with leaf explants followed by antibiotic selection on regeneration medium. Transplastomes were successfully screened, and the transformation efficiency of 3 transgenic lines from 25 bombarded leaf explants was determined. Transplastomic lines were evaluated by PCR and Southern blotting for the confirmation of aadA insertion and its integration into the chloroplast genome. Seeds collected from transplastomes were analyzed on spectinomycin medium with wild types to determine genetic stability. The increased chloroplast transformation efficiency (3 transplastomic lines from 25 bombarded explants) would be useful for expressing therapeutically and industrially important genes in Scoparia dulcis L.

• The Plant Pathology Journal
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• 제20권2호
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• pp.158-163
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• 2004

Chlorella is a eukaryotic microalgae which shares metabolic pathways with higher plants. These charac-teristics make chlorella a potential candidate for eukaryotic overexpression systems. Recently, a foreign flounder growth hormone gene was stably introduced and expressed in transformed Chlorella ellipsoidea by using a modified plant transformation vector that contains cauliflower mosaic virus (CaMV) 35S pro-moter and the phleomycin resistant Sh ble gene as a selection marker. In this study, this same vector was modified by incorporating a promoter and a 3' UTR region of the 33kDa peptide gene from a chlorella virus that was isolated in our laboratory. The 33kDa gene promoter was used to replace the 35S promoter and the 3' UTR was introduced to separate the target gene and downstream Sh ble gene. Three different chlorella transformation vectors containing human erythropoietin (EPO) gene were constructed. The mp335EPO vector consists of a promoter from the 33kDa peptide gene, whereas the mp3353EPO vector contains the same promoter from the 33kDa peptide gene and its 3' UTR. The mp35S33pEPO vector contains the 35S promoter and the 3' UTR from the 33 kDa peptide gene. There was no significant difference in the expression levels of EPO protein in chlorella cells transformed with either of three of the transformation vectors. These data indicate that the promoters from the chlorella virus are comparable to the most common CaMV 35S promoter. Furthermore, these data suggest that other promoters from this virus can be used in future construction of chlorella transformation system for higher expression of target proteins.

• 미생물학회지
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• 제27권2호
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• pp.92-97
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• 1989

the vector, pGUR19, for Rhizobium gene manipulation, was constructed by combining the replication and mobilization function of indigenous multicopy plasmid from Acacia(Robinia pseudoacacia L.) Rhizobia sp86 with E. coli cloning vehicle, pBR322. The vector could be efficiently mobilized by RP4 tra function incorporated into chromosome of E. coli named SM10 and efficiently transferred to various gram negative hosts including Rhizobium and Afrobacterium by transformation. Mobilization frequency of the constructed vector was ranged from $1.2\times 10^{-2}$ (E.coli HB 101) to $4.6\times 10^{-4}$ (A. tumefaciens 15955) and transformation frequency was ranged from $5.4\times 10^{-7}$(E. coli HB101) to $1.2\times 10^{-10}$ (A. tumefaciens 15955). The vector, pGUR19, was stably replicated and maintained in a variety of Rhizobium and Agrobacterium.

• Journal of international Conference on Electrical Machines and Systems
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• 제3권2호
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• pp.116-120
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• 2014

3-phase Induction machines are being used in industry and dq transformation from 3 phase of a, b, c is commonly used to analyze these machines. The equivalent circuits of d and q axis are however generally cross coupled and not simple to analyze. In this study, an analysis method of 3ph induction motor considering current regulator using dq transformation and matrix vector is proposed and it can explain the 3ph induction motor physically. This model does not need the separating process of d and q components. With this technique, the model becomes simple, is easy to understand in physical, and can get the same results with those from the other dq models. These simulation results of the proposed model are compared with those of other models for the conformation of the proposed method.

• 전기학회논문지
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• 제61권11호
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• pp.1601-1605
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• 2012

Three-phase voltage and current is applied to the three-phase alternating current motors which are commonly used in industry. Three phase variables of a, b, c are converted into d, q, 0 axis and the AC machines are modeled and analyzed. Basically the coordinate transformation or d-q transformation is used for convenience, a few steps are needed to analyze the motor performances - separating d and q components, establishing each equivalent circuit, and solving the differential equations of the circuits. In this study, a modeling technique of induction motor using complex vector is proposed and it can explain the induction motor physically. This method does not need the separating process of d and q components. With this technique, the model becomes simple, is easy to understand in physical, and can get the same results with those from the other models. These simulation results of the proposed model are compared with them for the conformation of the proposed method.

• 조명전기설비학회논문지
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• 제27권8호
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• pp.98-104
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• 2013

AC machines are in wide use in industry and d-q transformation from 3 phase of a, b, c is commonly used to analyze these kinds of machines. The equivalent circuits of d and q axis are, however, generally cross coupled and difficult to analyze. In this study, a modeling technique of AC machine including induction and PM synchronous motors using matrix vector is proposed. With that model, it can not only explain the AC machines physically but also make it simple to analyze them. The separating process of d and q components is not needed in this model and this model can be applied to analyze asymmetric motors like IPMSM machine. With this technique, the model becomes simple, easy to understand physically, and yields results that are the same as those from other models. These simulation results of the proposed model for induction motor are compared with those of other models to verify the method proposed.