• Microbiology and Biotechnology Letters
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• v.13 no.4
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• pp.345-348
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• 1985

Recombinant chimeric plasmid constructed with Xba I digested pUBl10 and -pE194 was transformed by polyethylene glycol induced protoplast transformation system into Bacillus subtilis BR 151 on the mannitol regeneration media, and two genes of antibiotics resistance were expressed simultaneously in the transfromant. Transformation frequency of the recombinant plasmid was 6.5 $\times$ 10$^{-5}$ on the mannitol regeneration agar plate containing neomycin and erythromycin. The replication of recombinant plasmid in the recipient cells was confirmed by the alkaline extraction method and agarose gel electrophoresis.

• The Journal of Asian Finance, Economics and Business
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• v.8 no.2
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• pp.1091-1099
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• 2021

The purpose of this research is to examine the effect of Servant Leadership and Business Transformation on Corporate Competitiveness and Corporate Sustainability. In this research, Service Innovation is the mediating element. The sample of this study was made up of all ship leaders in all crossings of PT ASDP Indonesia Ferry (Persero), which consisted of 150 Commanders or Chief Commanders (Mualim I). The location of the research is the Vessel PT. ASDP Indonesia Ferry (Persero) is used on all crossings of the company. It is a survey research using questionnaires given to 150 respondents. The validity and reliability were checked using Pearson correlation and Cronbach's Alpha. The analysis used the WarpPLS statistical method and WarpPLS software. The results from this research show that the increased Servant Leadership will improve Service Innovation. Business Transformation developed by PT ASDP has not been able to improve Service Innovation. To our knowledge, there is no study on the concept of comprehensive competitiveness and sustainability involving Servant Leadership, Business Transformation, and Service Innovation in an integrated and simultaneous manner. Therefore, the novelty of this research is the development of concepts or models of Corporate Competitiveness and Corporate Sustainability Theory through Servant Leadership, Business Transformation, and Service Innovation.

• 한국방재학회:학술대회논문집
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• 2007.02a
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• pp.534-537
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• 2007

The soil nailing was generally using method in practical business, in application of the soil nailing, the analysis was primarily used to Beam-Colum Method, Finite Element Method and Limit Equilibrium Method. Beam-Colum Method and Finite Element Method were able to examine transformation but widely using Limit Equilibrium Method wasn't able to examine transformation and displacement Therefore, this study was focused on presenting stability in comparison with former study-results about horizontal displacement of the soil nailing retaining-walls satisfing a criterion safety factor of Limit Equilibrium. There were performing comparison field measurements and former study-results in first step.

• Structural Engineering and Mechanics
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• v.43 no.6
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• pp.761-769
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• 2012

In this paper the Modified Finite Element-Transfer Matrix Method, which is the combination of Transfer Matrix Method and Finite Element Method, is applied to the static analysis of the structures. In the method, the structure is divided into substructures thus the number of unknowns that need to be worked out is reduced due to the transformation process. The static analysis of the structures can be performed easily and speedily by the proposed method. At the end of the study examples are presented for ensuring the agreement between the proposed method and classic Finite Element Method.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.20 no.6
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• pp.553-563
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• 2008

If an arbitrary topography is approximated to a number of vertical steps, both variational approximation and eigenfunction expansion method can be used to compute linear wave transformation over the bottom. In this study a scatterer method associated with variational approximation is proposed to calculate reflection and transmission coefficients. Present method may be shown to be more simple and direct than the successive-application-matrix method by O'Hare and Davies. And Several numerical examples are given which are in good agreement with existing results.

• Journal of the Korean Society for Precision Engineering
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• v.8 no.2
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• pp.47-56
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• 1991

The specifications needed for the mobile cranes are summarized as the following : 1) there may be not occured the oscillation of the cargo at unloading point. 2)the required time from departure point to destination point may be as short as possible. 3) there may be not a collapse of cargo caused by the oscillation in the course that the crago is mobilling. In this paper, the linear fractional transformation method is adopted as a method in order to improve the above mentioned problems. A design method of servo system is developed by modifying Davison's method for the case that the homogeneous differential equations of reference input and disturbance are different types. The real time control of a mobile crane system is implemented by 16bits microcomputer with A/D and D/A converters to illustrate the application of the adopted method. The experimental results for the three types of the design methods; linear fractional transformation method, servo system design method and optimal control method are shown for the comparison.

• Journal of architectural history
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• v.18 no.2
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• pp.47-63
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• 2009

This study focused on changes in form and spatial uses of Urban Hanok in Bukchon, Seoul. There are 10 representative cases which have been renovated through the policy of 'Preservation & Regeneration of Bukchon' by Seoul metropolitan government and other experts. Changes in form and spatial uses of Urban Hanok in Buckon are as follows. First, Changes of scale. Trough removing extension parts, facade of renovated Hanoks are 'transformed' into recovering their identity. Using basements or lofts, intensive application of spaces is transformation which promotes the vitality of Hanoks. Second, changes of space organization. As Hanok changes its function from residence to commercial or cultural use, il a1so changes space character or reorganizes space organization. It is important that deciding function of Hanok has to adjust its scale and organization. Third, changes of construction performance. Through introducing new material and constructing method, performance of wall has been changed respecting its wooden structure and interior-exterior figure. However, technical studies must back it up not to destroy its value of eco-friendly architecture. Fourth, changes of facility systems, like floor heating system. They changes floor level of Hanok equally, and then sections of Hanok have became simple. Furthermore, inserting new facility space, such as boiler room, stand-up kitchen, bathroom and toilet, organization of space also changed. It is necessary that wise alternative proposal through the method of transformation or mutation must be presented. These four changes can be classification into method of 'transformation' and 'mutation'. Changes of scale are method of transformation and changes of space organization are method of mutation. Also, while changes of construction performance are mutation, changes of facility systems are transformation. Recently, as price of lots have been increased, a lot of Hanoks have been commercialized. Thc commercial energy threat 'the identity of Bukchon as residential area'. From now on, to operate 'identity' and 'vitality' complementary, it is necessary to make up for the preservation policy of Hanok and consolidate renovating standards of Hanok which correspond to character of particular region and building usage.

• Journal of Korean Society of Steel Construction
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• v.18 no.1
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• pp.1-10
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• 2006

paper presents the results of the use of the differential transformation technique in analyzing the free vibration of circular plates.calculations were carried out and were compared with previously published results. The results that were obtained when this method was used coincide with the results of The present analysis shows the usefulness and validity of differential transformation in solving a solid-circular and annular-plate problem in terms of free-vibration responses.

• International Journal of Control, Automation, and Systems
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• v.5 no.6
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• pp.652-662
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• 2007

This paper presents a new and simple method for sensorless operation of matrix converter drives using a constant air-gap flux and the imaginary power flowing to the motor. To improve low-speed sensorless performance, the non-linearities of a matrix converter drive such as commutation delays, turn-on and turn-off times of switching devices, and on-state switching device voltage drop are modeled using PQR transformation and compensated using a reference current control scheme. The proposed compensation method is applied for high performance induction motor drives using a 3 kW matrix converter system. Experimental results are shown to illustrate the feasibility of the proposed strategy.

• Proceedings of the KIEE Conference
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• 1990.11a
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• pp.413-416
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• 1990

This paper proposes a design procedure based on the LQG/LTR method for a launch vehicle autopilot. Continuous-discrete type LQG/LTR compensators are designed using the $\delta$-transformation [1] in order to overcome numerical problems occurring in the process of discretization. The $\delta$-LQG/LTR compensator using the $\delta$-transformation is compared with the $\delta$-LQG/LTR compensator using the $\delta$-transformation. The performance of the overall system controlled by the $\delta$-LQG/LTR compensator is evaluated via simulations, which show that the discretization error problem is resolved and the control performances are satisfied in the proposed compensator.