• 제목/요약/키워드: transformation method

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재조합 Plasmid DNA에 의한 Bacillus subtilis의 형질전환 (Transformation of Bacillus subtilis Protoplast by Recombinant Plasmid DNA)

  • Kim, Sang-Dal;John Spizizen
    • 한국미생물·생명공학회지
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    • 제13권4호
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    • pp.345-348
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    • 1985
  • Mannitol hypertonic regeneration media를 사용하는 PEG-induced protoplast transformation system을 이용해서 pUB110과 pE194의 recombinant plasmid로 B. subtilis BR151을 transformation 시킴으로써 두 plasmid에서 유래되는 각각의 Neo$^{R}$와 Em$^{R}$을 동일한 recipient cell 내에서 동시에 발현시킬 수 있었다. Neomycin과 erythromycin을 함께 함유하는 mannitol regeneration media상에서 recombinant plasmid의 transformation frequency는 6.5 $\times$ $10^{-5}$이었다. 한편 transformant cell 내에서 recombinant plasmid의 replication이 agarose gel electrophoresis로 확인되었다.

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Servant Leadership, Business Transformation, and Corporate Competitiveness

  • HUTABARAT, Christine;SUHARYONO, Suharyono;UTAMI, Hamidah Nayati;PRASETYA, Arik
    • The Journal of Asian Finance, Economics and Business
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    • 제8권2호
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    • pp.1091-1099
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    • 2021
  • The purpose of this research is to examine the effect of Servant Leadership and Business Transformation on Corporate Competitiveness and Corporate Sustainability. In this research, Service Innovation is the mediating element. The sample of this study was made up of all ship leaders in all crossings of PT ASDP Indonesia Ferry (Persero), which consisted of 150 Commanders or Chief Commanders (Mualim I). The location of the research is the Vessel PT. ASDP Indonesia Ferry (Persero) is used on all crossings of the company. It is a survey research using questionnaires given to 150 respondents. The validity and reliability were checked using Pearson correlation and Cronbach's Alpha. The analysis used the WarpPLS statistical method and WarpPLS software. The results from this research show that the increased Servant Leadership will improve Service Innovation. Business Transformation developed by PT ASDP has not been able to improve Service Innovation. To our knowledge, there is no study on the concept of comprehensive competitiveness and sustainability involving Servant Leadership, Business Transformation, and Service Innovation in an integrated and simultaneous manner. Therefore, the novelty of this research is the development of concepts or models of Corporate Competitiveness and Corporate Sustainability Theory through Servant Leadership, Business Transformation, and Service Innovation.

현장계측에 의한 쏘일네일링 보강벽체의 변형특성에 관한 고찰 (A Consideration on Deformation Characteristics of Soil Nailed Retaining-Walls on Field Measurements)

  • 윤배식;이종문;강인규;권영호;김홍택
    • 한국방재학회:학술대회논문집
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    • 한국방재학회 2007년도 정기총회 및 학술발표대회
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    • pp.534-537
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    • 2007
  • The soil nailing was generally using method in practical business, in application of the soil nailing, the analysis was primarily used to Beam-Colum Method, Finite Element Method and Limit Equilibrium Method. Beam-Colum Method and Finite Element Method were able to examine transformation but widely using Limit Equilibrium Method wasn't able to examine transformation and displacement Therefore, this study was focused on presenting stability in comparison with former study-results about horizontal displacement of the soil nailing retaining-walls satisfing a criterion safety factor of Limit Equilibrium. There were performing comparison field measurements and former study-results in first step.

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Modified finite element-transfer matrix method for the static analysis of structures

  • Ozturk, D.;Bozdogan, K.;Nuhoglu, A.
    • Structural Engineering and Mechanics
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    • 제43권6호
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    • pp.761-769
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    • 2012
  • In this paper the Modified Finite Element-Transfer Matrix Method, which is the combination of Transfer Matrix Method and Finite Element Method, is applied to the static analysis of the structures. In the method, the structure is divided into substructures thus the number of unknowns that need to be worked out is reduced due to the transformation process. The static analysis of the structures can be performed easily and speedily by the proposed method. At the end of the study examples are presented for ensuring the agreement between the proposed method and classic Finite Element Method.

변분근사식과 연계된 산란체법에 의한 파랑변형 계산 (Computation of Wave Propagation by Scatter Method Associated with Variational Approximation)

  • 서승남
    • 한국해안·해양공학회논문집
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    • 제20권6호
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    • pp.553-563
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    • 2008
  • 만일 임의의 지형을 다수의 계단으로 근사하면 이 지형 위를 지나는 선형 파랑의 변형을 계산하기 위해 변분근사법과 고유함수 전개법을 사용할 수 있다. 본 논문에서는 반사율과 투과율을 계산하기 위해 변분근사식과 연계된 산란체법을 제시하였다. 본 기법은 O'Hare and Davies의 변환행렬 축차법보다 간단하고 직접적인 방법임을 보였다. 또한 수 개의 수치실험을 실시하여 기존 결과와 거의 같은 결과를 얻었다.

이동 CRANE의 제어에 관한 연구 (A Study on Control of Mobile Cranes)

  • 김상봉;신민생;김환성;정용길
    • 한국정밀공학회지
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    • 제8권2호
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    • pp.47-56
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    • 1991
  • The specifications needed for the mobile cranes are summarized as the following : 1) there may be not occured the oscillation of the cargo at unloading point. 2)the required time from departure point to destination point may be as short as possible. 3) there may be not a collapse of cargo caused by the oscillation in the course that the crago is mobilling. In this paper, the linear fractional transformation method is adopted as a method in order to improve the above mentioned problems. A design method of servo system is developed by modifying Davison's method for the case that the homogeneous differential equations of reference input and disturbance are different types. The real time control of a mobile crane system is implemented by 16bits microcomputer with A/D and D/A converters to illustrate the application of the adopted method. The experimental results for the three types of the design methods; linear fractional transformation method, servo system design method and optimal control method are shown for the comparison.

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서울 북촌한옥의 변화양상에 관한 연구 - 북촌 가꾸기 사업에 따른 2002~2007 한옥 대수선 사례를 대상으로 - (A study on the Changes in form and spatial uses of Urban Hanok in Bukchon, Seoul)

  • 송인호;김영수;조은주
    • 건축역사연구
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    • 제18권2호
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    • pp.47-63
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    • 2009
  • This study focused on changes in form and spatial uses of Urban Hanok in Bukchon, Seoul. There are 10 representative cases which have been renovated through the policy of 'Preservation & Regeneration of Bukchon' by Seoul metropolitan government and other experts. Changes in form and spatial uses of Urban Hanok in Buckon are as follows. First, Changes of scale. Trough removing extension parts, facade of renovated Hanoks are 'transformed' into recovering their identity. Using basements or lofts, intensive application of spaces is transformation which promotes the vitality of Hanoks. Second, changes of space organization. As Hanok changes its function from residence to commercial or cultural use, il a1so changes space character or reorganizes space organization. It is important that deciding function of Hanok has to adjust its scale and organization. Third, changes of construction performance. Through introducing new material and constructing method, performance of wall has been changed respecting its wooden structure and interior-exterior figure. However, technical studies must back it up not to destroy its value of eco-friendly architecture. Fourth, changes of facility systems, like floor heating system. They changes floor level of Hanok equally, and then sections of Hanok have became simple. Furthermore, inserting new facility space, such as boiler room, stand-up kitchen, bathroom and toilet, organization of space also changed. It is necessary that wise alternative proposal through the method of transformation or mutation must be presented. These four changes can be classification into method of 'transformation' and 'mutation'. Changes of scale are method of transformation and changes of space organization are method of mutation. Also, while changes of construction performance are mutation, changes of facility systems are transformation. Recently, as price of lots have been increased, a lot of Hanoks have been commercialized. Thc commercial energy threat 'the identity of Bukchon as residential area'. From now on, to operate 'identity' and 'vitality' complementary, it is necessary to make up for the preservation policy of Hanok and consolidate renovating standards of Hanok which correspond to character of particular region and building usage.

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가변두께를 가지는 원판의 진동해석에 관한 연구 (Vibration Analysis of Circular Plate with Continuously Varying Thickness)

  • 신영재;전수주;윤종학;유영찬
    • 한국강구조학회 논문집
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    • 제18권1호
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    • pp.1-10
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    • 2006
  • 본 논문은 원형 판의 진동 해석에 미분변환법을 적용하였다. 계산된 수치적인 결과들은 이전의 연구결과들과 비교되었다. 그 결과를 기존의 연구 결과와 비교하여 미분변환법의 타당성을 검증하였고, 두께 형상과 경계조건 및 내경변화에 의한 고유진동수의 변화를 해석 및 고찰하였다. 유용성을 입증하였다.

A Simple Sensorless Scheme for Induction Motor Drives Fed by a Matrix Converter Using Constant Air-Gap Flux and PQR Transformation

  • Lee, Kyo-Beum;Blaabjerg, Frede
    • International Journal of Control, Automation, and Systems
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    • 제5권6호
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    • pp.652-662
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    • 2007
  • This paper presents a new and simple method for sensorless operation of matrix converter drives using a constant air-gap flux and the imaginary power flowing to the motor. To improve low-speed sensorless performance, the non-linearities of a matrix converter drive such as commutation delays, turn-on and turn-off times of switching devices, and on-state switching device voltage drop are modeled using PQR transformation and compensated using a reference current control scheme. The proposed compensation method is applied for high performance induction motor drives using a 3 kW matrix converter system. Experimental results are shown to illustrate the feasibility of the proposed strategy.

LQG/LTR 기법에 의한 발사체의 자동조종장치 설계 (Autopilot Design Using LQG/LTR Method for a Launch Vehicle)

  • 김승환;권오규
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 1990년도 추계학술대회 논문집 학회본부
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    • pp.413-416
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    • 1990
  • This paper proposes a design procedure based on the LQG/LTR method for a launch vehicle autopilot. Continuous-discrete type LQG/LTR compensators are designed using the $\delta$-transformation [1] in order to overcome numerical problems occurring in the process of discretization. The $\delta$-LQG/LTR compensator using the $\delta$-transformation is compared with the $\delta$-LQG/LTR compensator using the $\delta$-transformation. The performance of the overall system controlled by the $\delta$-LQG/LTR compensator is evaluated via simulations, which show that the discretization error problem is resolved and the control performances are satisfied in the proposed compensator.

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