• Proceedings of the Microbiological Society of Korea Conference
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• 2000.10a
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• pp.85-91
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• 2000

Aspergillus oryzae is a filamentous fungus classified in the group Aspergillaceae Ascomycetes. It is an important microorganism for industrial production of enzymes and fermented food productions. It secrets large quantities of proteins or enzymes into the culture medium which makes this organism appealing for the production of heterologous proteins. Recently Electric field-mediated transformation method, electroporation, has been applied to fungal transformation. In this study, fungal transformation was carried out by bypassing the protoplast isolation step, decreasing the culturing time and non-protoplast transformation for the increment of transformation efficiency. Transformants were obtained with electroporation in optimal condition 2,500 voltage, 1,540 ohm and 0.50 capacitance. More than 1,000 transform ants were obtained with 6-10 hrs cultured mycelia without enzyme treatment, called non-protoplast transformation.

• The Journal of Advanced Prosthodontics
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• v.11 no.5
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• pp.253-261
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• 2019

PURPOSE. To evaluate the influence of cyclic loading on phase transformation of zirconia abutments and to compare the effectiveness of three different quantitative ageing assessment techniques. MATERIALS AND METHODS. Thirty two Y-TZP prostheses fabricated from two brands, InCoris ZI and Ceramill ZI, were cemented to titanium bases and equally divided into two subgroups (n=8): control group without any treatment and aged group with cyclic loading between 20 N and 98 N for 100,000 cycles at 4 Hz in distilled water at $37^{\circ}C$. The tetragonal-to-monoclinic phase transformation was assessed by (i) conventional x-ray diffraction (XRD), (ii) micro x-ray diffraction (${\mu}XRD$), and (iii) micro-Raman spectroscopy. The monoclinic-phase fractions (M%) were compared by two-way ANOVA. RESULTS. InCoris Zi presented significantly higher M% than Ceramill Zi in both control and aged groups (P<.001). Both materials exhibited significant phase transformation with monoclinicphase of 1 to 3% more in aged groups than controls for all three assessment techniques. The comparable M% was quantified by both ${\mu}XRD$ and XRD. The highest M% was assessed with micro-Raman. CONCLUSION. Cyclic loading produced significant phase transformation in tested Y-TZP prostheses. The micro-Raman spectroscopy could be used as an alternative to XRD and ${\mu}XRD$.

• Structural Engineering and Mechanics
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• v.85 no.5
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• pp.645-654
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• 2023

Based on first-order shear deformation theory, a wave propagation model of graphene platelets reinforced metal foams (GPLRMFs) circular plates is built in this paper. The expressions of phase-/group- velocities and wave number are obtained by using Laplace integral transformation and Hankel integral transformation. The effects of GPLs pattern, foams distribution, GPLs weight fraction and foam coefficient on the phase and group velocity of GPLRMFs circular plates are discussed in detail. It can be inferred that GPLs distribution have great impacts on the wave propagation problems, and Porosity-I type distribution has the largest phase velocity and group velocity, followed by Porosity-III, and finally Porosity-II; With the increase of the GPLs weight fraction, the phase- and group- velocities for the GPLRMFs circular plate will be increased; With the increase of the foam coefficient, the phase- and group- velocities for the GPLRMFs circular plate will be decreased.

• Journal of the Korean Mathematical Society
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• v.27 no.1
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• pp.111-117
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• 1990

• Communications of the Korean Mathematical Society
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• v.6 no.1
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• pp.135-143
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• 1991

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.215-218
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• 2000

Aspergillus oryzae is a filamentous fungus classified in the group Aspergillaceae Ascomycetes. It is an important microorganism for industrial production of enzymes and fermented food productions. The genetic transformation system in A. oryzae was used to protoplast mediated transformation with PEG/$CaCl_2$. When the protoplast was used, the regeneration efficiency was decreased and then transformation frequence was also effected. In this study, fungal transformation was carried out by bypassing the protoplast isolation step, changing enzymes, such as hemicellulase and celluclast, and decreasing the culturing time for the increment of the transformation efficiency. 83 transformants/10ug of DNA with hemicellulase were obtained, compared with less than 10 transformants with novozyme234 and celluclast.

• Korean Journal of Mathematics
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• v.5 no.1
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• pp.49-54
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• 1997

In this paper we study the generalized Reidemeister number $R({\varphi},{\psi})$ for a self-map $({\varphi},{\psi}):(X,G){\rightarrow}(X,G)$ of a transformation group (X, G), as an extension of the Reidemeister number $R(f)$ for a self-map $f:X{\rightarrow}X$ of a topological space X.

• Proceedings of the KIEE Conference
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• 1997.07b
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• pp.422-424
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• 1997

In this paper, we characterize the whole class of vector fields that can be linearized by a given nominal state transformation and a feedback linearizing controller. The necessary and sufficient condition for a given uncertain vector field to be so-called "completely linearizable by the nominal coordinate transformation" is given in terms of Lie Bracket of uncertain vector fields and some suitable vector fields of the nominal system.

• Journal of the Chungcheong Mathematical Society
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• v.20 no.1
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• pp.31-35
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• 2007

In dealing with transformation group, topological approach is very natural. But, it is not sufficient to investigate geometric properties of transformation group and we need geometric method. Frankel-McDuff Conjecture is very interesting in the point that it shows struggling between topological method and geometric method. In this paper, the author suggest generalized Frankel-McDuff conjecture as a topological version of the conjecture and construct a counterexample for the generalized version, and from this we assert that topological method does not work for Frankel-McDuff Conjecture.

• The Journal of Pediatrics of Korean Medicine
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• v.21 no.1
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• pp.189-209
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• 2007

Objectives : In order to study the effect of Dangkwiyughwangtang and Okbyoungpoongsangamibang on the immune response induced by methotrexate in mice. Method : Delayed type of hypersensitivity, hemagglutinin titer, hemolysin titer, rosette forming cells, phagocytic activity for immune response, lymphocyte transformation, and productivity of Interleukin-2 were measured. Results : Body weight decreasing was significantly inhibited as compared with control group in both the Dangkwiyughwangtang and Okbyoungpoongsangamibang groups. Delayed type of hypersensitivity was significantly increased as compared with control group in both groups Hemagglutinin titer was significantly increased as compared with control group in both groups. Hemolysin titer was significantly increased as compared with control group in the Okbyoungpoongsangamibang group. Rosette forming cells were significantly increased as compared with control group in both groups. Phagocytic activity for immune response was slightly decreased in the Dangkwiyughwangtang group and slightly increased in the Okbyoungpoongsangamibang group insignificantly as compared with the control group. Lymphocyte transformation was significantly increased as compared with the control group in both the Dangkwiyughwangtang and Okbyoungpoongsangamibang groups. Productivity of Interleukin-2 was significantly increased as compared with the control group in both the Dangkwiyughwangtang and Okbyoungpoongsangamibang groups. Conclusion : Both the Dangkwiyughwangtang and Okbyoungpoongsangamibang groups enhance immunity in mice.