• 대한화학회지
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• 제46권5호
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• pp.432-449
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• 2002

본 논문에서는 산화 환원 효소의 최적 pH를 예측해 보았다. Boltzman 분배를 이용하여 어떤 아미노산의 side chain이 물에서 발견될수 있는 상대적 확률이나 물에 대한 상대적 친화력을 구하였으며 p$K_R$을 이용해 protonated 아미노산의 양과 deprotonated 아미소산의 양을 계산하였다. 효소의 최적 pH는 아미노산의 side chain이 물에서 발견될수 있는 상대적 확률과 protonated 또는 deprotonated된 아미노산 양의 곱인 유효 protonated된 양과 유효 depotonated된 양이 같아지는 pH로 예측하였다. 문헌 값과 비교해 보았을 때 예측치는 상당히 일치하는 경향을 보였으며 이 결과 로 효소 자체의 전도도가 생물학적 기능에 있어 매우 중요한 역할을 하는 것을 알 수있다.

• 한국수정란이식학회지
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• 제22권4호
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• pp.257-263
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• 2007

This study was performed to investigate the relationship between PSS-HSP70 gene polymorphism and artificial insemination (AI) reproductivity in the pigs. The RFLP polymorphism of PSS and the SSCP polymorphisms of HSP70 K1, K3 and K4 PCR product were detected different patterns. In the experiment for AI of fresh semen, spring and fall season showed higher litter size born of 10.89 head than 10.47 head of summer season. Landrace was showed higher litter size of 9.96 head than that of Duroc and Yorkshire (p<0.05). Stress relating PSS and HSP70 polymorphism of PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showd a highest litter size born of 10.97 head and litter size born alive of 10.69 head than that of the other polymorphisms(p<0.05). In the experiment for AI of frozen semen, effects of season and pig breeds were not showed for litter size born. The stress relating polymorphism of PSS-Carrier, HSP70 K1-BB, K3-BB, K4-AB showed highest litter size born of 11.29 head and litter size born alive of 10.82 head and PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showed the lowest litter size born of 8.48 head and litter size born alive of 7.33 head than that of the other polymorphisms(p<0.05). These results suggest that AI litter size born for the stress of forzen thawed semen may be affected by PSS and HSP70 polymorphism in pigs.

• Bulletin of the Korean Chemical Society
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• 제7권5호
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• pp.395-399
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• 1986

Intramolecular proton transfer equilibria of acetamide and methyl carbamate have been studied theoretically by MNDO MO method. For both substrates, carbonyl-O protonated tautomer was found to be the most stable form, the next most stable one being N-protonated form. Gas phase proton transfers take place by the 1,3-proton rearrangement process and in all cases have prohibitively high activation barriers. When however one solvate water molecule participates in the process, the barriers are lowered substantially and the process proceeds in an intermolecular manner through the intermediacy of the water molecule via a triple-well type potential energy surface; three wells correspond to reactant(RC), intermediate(IC) and product complex(PC) of proton donor-acceptor pairs whereas two transition states(TS) have proton-bridge structure. General scheme of the process can be represented for a substrate with two basic centers(heteroatoms) of A and B as, $$ABH\limits^+\;+\;H_2O\;{\to}\;ABH\limits^+{\cdots}{\limits_{RC}}OH_2\;{\to}\;AB{\cdots}H\limits_{TS}^+{\cdots}{\limits_{1}}OH_2\;{\to}\;AB{\cdots}{\limits_{IC}}H\limits^+OH_2\;{\to}\;BA{\cdots}H\limits_{TS}^+{\cdots}{\limits_{2}}OH_2\;{\to}\;BA H\limits^+{\cdots}{\limits_{PC}}OH_2\;{\to}\;BAH\limits^+\;+\;H_2O$$ Involvement of a second solvate water had negligible effect on the relative stabilities of the tautomers but lowered barrier heights by 5∼6 Kcal/mol. It was calculated that the abundance of the methoxy-O protonated tautomer of the methyl carbamate will be negligible, since the tautomer is unfavorable thermodynamically as well as kinetically. Fully optimized stationary points are reported.

• 한국소프트웨어감정평가학회 논문지
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• 제17권1호
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• pp.11-16
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• 2021

정보통신의 발달로 인하여 소프트웨어의 중요성이 증대되고 있으며, 이에 따른 소프트웨어 저작권 분쟁도 증가하는 추세에 있다. 본 논문은 제출된 프로그램들의 소스와 관련하여 프로그램 수행에 필요한 파일들을 감정범위로 하였다. 분석 대상인 대용량 파일 전송 솔루션 프로그램은 데이터에 대한 전자서명 및 암호화를 통하여 기밀성, 무결성, 사용자 인증, 부인방지 기능 등의 부가 기능을 제공하고 있다. 본 논문에서는 프로그램 A, 프로그램 B, 프로그램 C 3개에 대하여 분석을 수행한다. 프로그램 유사율을 산출하기 위하여 다음과 같은 내용을 분석한다. 패키지의 구조, 패키지 이름, 각 패키지 내 소스파일 이름, 소스파일 내 변수명, 함수명, 함수구현 소스코드, 제품의 환경변수 정보에 대하여 유사 여부를 분석하고 프로그램의 전체 유사율을 산출한다. 패키지 구조 및 패키지 이름이 일치되는 정도를 확인하기 위해, 폴더 구조를 비교하여 유사도 판단을 하였다. 또한 패키지 구조 및 패키지 이름이 어느 정도 일치하는지와 각 패키지 내 소스 파일(클래스) 이름이 어느 정도 일치하는지에 대한 분석을 하였다.

• 한국수정란이식학회:학술대회논문집
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• 한국수정란이식학회 2002년도 국제심포지엄
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• pp.99-99
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• 2002

The hormone resistin is associated with typeII diabetes mellitus in rodent model. Resistin impairs glucose tolerance and insulin action. A new class of anti-diabetic drugs were called thiazolidinediones (TZDs) downregulates a resistin which is induced during adipocyte differentiation. But the connection between increased adiposity and resistin remains unknown. The objectives of this study was to clone a mouse resistin cDNA and to generate transgenic mice overexpressing mouse resistin gene. The 555 bp of mouse resistin was amplified from mob cDNAS by polymerase chain reaction (PCR) and cloned into pCR$\^$(R)/ 2.1 TOPO T-vector. Mouse resistin mRNA on the basis of Genbank sequence (acession no. AF323080). Then, the PCR product was cloned into pTargeT$\^$TM/ mammalian expression vector that has pCMV promoter and chimeric intron. Restriction enzyme analysis with BamH I and Not I was carried out to determine an orientation of the insert in the vector. The pCMV-mus/resistin gene was prepared from previous recombinant pTargeT$\^$TM/-mus/resistin by digestion of Bgl II, and has used for microinjection into pronuclei of one cell embryos. The microinjected embryos were transfered to pseudopregnant foster-mother. Mouse resistin expression was detected in transgenic F1 mice by Reverse Transcriptase- Polymerase Chain Reaction (RT-PCR). Resistin gene expression mouse has heavier body weight which was measured higher level of plasma glucose than that of normal mouse. And in diet-induced experiments, the abdominal fat pads were isolated from each 24h starvation and re-feeding after fasting group mice that were assessed by RT-PCR analysis. In fasting group mice, resistin expression was higher than that of re-feeding group mice. This result suggests that the resistin gene overexpressing mice may be became to obesity and be useful as an animal disease model to be diabetes mellitus caused by insulin resistance of resistin.

• 한국의상디자인학회지
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• 제13권1호
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• pp.135-146
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• 2011

Despite the recession that has affected people's consumption of clothing, the 'Gold Kids' market shows steady growth. This study analyzed the characteristics of components that make up infant and children's wear brands. Firstly, this study found the linguistic features of infant and children's clothing brands. Most brand names have common characteristics such as idiomatic expressions, combination and separation of vocabulary, and more than 6 syllables. Also, when they formative brand quality, they frequently used words like 'girl', 'kid' and 'baby' to clarify the category of the brand product. Also it is meant to present a clear brand concept to the target consumer to offer their brand information and product characteristics. Moreover, English is widely used in the brand names, and they used appropriate symbol graphics that fit the titles and qualities of the brands. Second, to strengthen their brand identity and inherent concepts, infant and children's clothing brands normally use their own particular brand colors wich makes special and strong visual impressions on the target consumer. Also, they mainly use applied sans serif typeface showing the softer brand quality. Brand logos have different visual expressions depending on their brand value and the target consumer. The word type of the logos presents clear brand identity and information to the target consumer. Through the study, we found that infant and children's clothing brands tend to use written brand names for the logo type more than graphic symbol logo patterns. This indicates that infant and children's clothing brands tend to emphasize information transfer rather than visual graphic symbols when they organize brand logos.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권4호
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• pp.299-305
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• 2006

Aromatic L-amino acid transaminase is an enzyme that is able to transfer the amino group from L-glutamate to unnatural aromatic ${\alpha}-keto$ acids to generate ${\alpha}-ketoglutarate$ and unnatural aromatic L-amino acids, respectively. Enrichment culture was used to isolate thermophilic Bacillus sp. T30 expressing this enzyme for use in the synthesis of unnatural L-amino acids. The asymmetric syntheses of L-homophenylalanine and L-phenylglycine resulted in conversion yields of >95% and >93% from 150 mM 2-oxo-4-phenylbutyrate and phenylglyoxylate, respectively, using L-glutamate as an amino donor at $60^{\circ}C$. Synthesized L-homophenylalanine and L-phenylglycine were optically pure (>99% enantiomeric excess) and continuously pre-cipitated in the reaction solution due to their low solubility at the given reaction pH. While the solubility of the ${\alpha}-keto$ acid substrates is dependent on temperature, the solubility of the unnatural L-amino acid products is dependent on the reaction pH. As the solubility difference between substrate and product at the given reaction pH is therefore larger at higher temperature, the thermophilic transaminase was successfully used to shift the reaction equilibrium toward rapid product formation.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.3113-3113
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• 2001

Some two years ago our company undertook a project on manufacturing network rationalization to maximize competitiveness through continuous improvement in manufacturing efficiency. One key outcome was the recognition of the benefits that could be derived from timely application of new technology or novel use of existing technologies and even more importantly the need to develop company wide strategies to maximize the impact of such applications. As a direct result an exercise was undertaken to identify the ten most promising technologies from a list of literally hundreds seen as having the capability of making a rapid impact on the manufacturing initiative. One of the outcomes of this exercise was the identification of Near Infrared Spectroscopy as a pivotal technology for improving process understanding, performance, and control to deliver consistent product quality cost effectively with broad applicability across our product range. While NIR had been in use in targeted areas on some of our sites for some years our new challenge was to develop a strategy to extend NIRs application, initially over 17 manufacturing sites, while concurrently expanding the NIR skill base company wide to ensure that the return on initial investment could be further maximized as shared applications across the remaining sites as required. This presentation will provide an overview of how life cycle based user requirement specifications were developed covering: ㆍSpectrophotometers ㆍSample interfaces ㆍSoftware ㆍEquipment and Software qualification ㆍCalibration transfer ㆍ Ease of developing effective user interfaces and control for applications transferred to a production area ㆍUser training ㆍWorld wide support The presentation will also describe the process adopted for vendor selection to ensure maximum utilization of the existing company wide NIR skill base and its future development to expedite applications of the technology in development, quality control and production areas.

• 대한산업공학회지
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• 제39권1호
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• pp.46-54
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• 2013

Recently, the marine leisure industry has begun to make a mark on the growth engines of the Korean economy. Hence, various government research projects are in progress to catch up with the fundamental yacht technologies of advanced countries. Research on the integrated management technologies of sail yachts is carried out as a part of those projects. Work Breakdown Structure (WBS) is a basic architecture for robust and high-level management systems. In this paper, we suggest a product-oriented Yacht Work Breakdown Structure (YWBS). General WBS has a drawback in that it can only follow the targeted purpose, but YWBS allows for a generic WBS approach. This concept allows us to apply WBS to multi-purpose uses. The YWBS is used as an infrastructure in information management and for data relationships in sail yacht management systems. YWBS is designed and managed by a web application, and the system is implemented with a website as a user interface. This application has not only a design function, but also a data transfer function that is based on the Microsoft Silverlight platform, a kind of Rich Internet Application (RIA) technology. Using this application, YWBS designers can communicate with the management system without added tasks like as uploading and updating.

• 전자공학회논문지C
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• 제36C권5호
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• pp.38-47
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• 1999

저전력을 실현하기 위하여 구조, 논리 및 트랜지스터레벨에서 16비트 덧셈기를 설계하였다. 기존의 ELM덧셈기는 입력 비트 패턴에 의해 계산되는 블록캐리발생신호 (block carry generation signal) 때문에 특정 입력 비트 패턴이 인가되었을 때에는 G셀에서 글리치(glitch)가 발생하는 단점이 있다. 따라서 구조레벨에서는 특정 입력 비트 패턴에 대해서 글리치를 피하기 위해 자동적으로 각각의 블록캐리발생신호를 마지막 레벨의 G셀에 전달하는 저전력 덧셈기 구조를 제안하였다. 또한, 논리레벨에서는 정적 CMOS(static CMOS)논리형태와 저전력 XOR게이트로 구성된 저전력 소모에 적합한 조합형 논리형태(combination of logic style)를 사용하였다. 게다가 저전력을 위하여 트랜지스터레벨에서는 각 비트 전파의 논리깊이(logic depth)에 따라서 가변 크기 셀들(variable-sized cells)을 사용하였다. 0.6㎛ 단일폴리 삼중금속 LG CMOS 표준 공정변수를 가지고 16비트 덧셈기를 HSPICE로 모의 실험한 결과, 고정 크기 셀(fixed-sized cell)과 정적 CMOS 논리형태만으로 구성된 기존의 ELM 덧셈기에 비해 본 논문에서 제안된 덧셈기가 전력소모면에서는 23.6%, power-delay-product면에서는 22.6%의 향상을 보였다.