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Relationship between Stress Gene Polymorphisms and Litter Size by AI in Pigs  

Jin, H.J. (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
Kim, I.C. (Swine Science Division, NIAS, RDA)
Wee, M.S. (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
Yeon, S.H. (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
Kim, C.D. (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
Lee, S.S. (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
Cho, C.Y. (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
Cho, S.R. (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
Son, D.S. (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
Park, C.K. (College of Animal Resource Science, Kangwon National University)
Li, Z.D. (College of Biological Science, China Agricultural University)
Publication Information
Journal of Embryo Transfer / v.22, no.4, 2007 , pp. 257-263 More about this Journal
Abstract
This study was performed to investigate the relationship between PSS-HSP70 gene polymorphism and artificial insemination (AI) reproductivity in the pigs. The RFLP polymorphism of PSS and the SSCP polymorphisms of HSP70 K1, K3 and K4 PCR product were detected different patterns. In the experiment for AI of fresh semen, spring and fall season showed higher litter size born of 10.89 head than 10.47 head of summer season. Landrace was showed higher litter size of 9.96 head than that of Duroc and Yorkshire (p<0.05). Stress relating PSS and HSP70 polymorphism of PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showd a highest litter size born of 10.97 head and litter size born alive of 10.69 head than that of the other polymorphisms(p<0.05). In the experiment for AI of frozen semen, effects of season and pig breeds were not showed for litter size born. The stress relating polymorphism of PSS-Carrier, HSP70 K1-BB, K3-BB, K4-AB showed highest litter size born of 11.29 head and litter size born alive of 10.82 head and PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showed the lowest litter size born of 8.48 head and litter size born alive of 7.33 head than that of the other polymorphisms(p<0.05). These results suggest that AI litter size born for the stress of forzen thawed semen may be affected by PSS and HSP70 polymorphism in pigs.
Keywords
HSP70 gene; PSS; AI; pig;
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1 Tsukimi Y and Okabe S. 2001. Recent advances in gastrointestinal pathophysiology: role of heat shock proteins in mucosal defense and ulcer healing. Biological and Pharmaceutical Bulletin, 24: 1-9   DOI   ScienceOn
2 Chowdbury AK and Steinberger E. 1964. A quantitative study of the effect of heat on germinal epithelium of rat testes. Am. J. Anat., 115: 509-524   DOI   ScienceOn
3 Leung SM, Senisterra G, Ritchie KP, Sadis SE, Lepock JR and Hightower LE. 1996. Thermal activation of the bovine HSC70 molecular chaperone at physiological temperatures: physical evidence of a molecular thermometer. Cell Stress Chaperones, 1: 78-89   DOI
4 Rothschild MF and Ruvinsky A. 1998. The genetics of the pig. CAB. INTERNATIONAL, pp. 135-197
5 Subjeck JR and Shyy TT. 1986. Stress protein systems of mammalian cells. Am. J. Physiol., 250 (1 Pt 1): C1-17   DOI   PUBMED
6 Welch WJ. 1992. Mammalian stress response: cell physiology, structure/function of stress proteins and implications for medicine and disease. Physiol. Rev., 72: 1063-1081   DOI   PUBMED
7 Wertemann RP, Wells ME, Omtvedt IT, Pope CE and Turman EJ. 1976. Influence of elevated ambient temperature on reproductive performance of boars. J. Anim. Sci., 42: 664-669   DOI   PUBMED
8 Kennedy BW and Wilkins IN. 1984. Boar, breed and environmental factors influencing semen characteristics of boars used in artificial insemination. Can. J. Anim. Sci. 64: 833-843   DOI
9 Nover L. 1991. HSFs and HSPs-a stressful program on transcription factors and chaperones. Stress Proteins and the Heat Shock Response, sponsored by Cold Spring Harbor Laboratory, Cold Spring Harbor, NY USA, April 29-May 2, 1991. New Biol., 3(9): 855-859
10 SAS Institute. 1989. SAS User's Guide: Statistics. Release 6.3 ed. SAS Institute Inc., Cary, NC, USA
11 Jin HJ, Park BY, Park JC, Hwang IH, Lee SS, Yeon SH, Kim CD, Cho CY, Kim YK, Min KS, Feng ST, Li ZD, Park CK and Kim CI. 2006. The effects of stress related genes on carcass traits and meat quality in pigs. Asian-Aust. J. Anim. Sci., 19(2):280-285
12 Fujii JK, Otsu F, Zorzato S, De Leon VK, Khanna JE, Weiler PJ, Brien O and Maclennan DH. 1991. Identification of a mutation in porcine ryanodine receptor associated with malignant hyperthermia. Science, 253: 448-451   DOI
13 Jin HJ, Kim IC, Wee MS, Yeon SH, Kim CD, Cho CY, Choi SH, Cho SR, Son DS, Kim YK, Jung JR, Choi HS, Park CK and Kim CI. 2005. Relationship between HSP70 gene Polymorphisnms and IVF embryo development in pigs. Korean J. Emb. Trans., 20(3):289-295   과학기술학회마을
14 Sanchez Y, Taulien J, Borkovich KA and Lindquist SL. 1992. HSPI04 is required for tolerance to many forms of stress. EMBO J., 11: 2357-2364   PUBMED
15 Li GC and Laszlo A. 1985. Thermotolerance in Mammalian Cells: A possible role for heat shock protein. In: Atkinson BG, Walden DB. (Eds.), Changes in Eukaryotic Gene Expression in Response to Environmental Stress. Academic Press, Orlando, FL, USA, pp. 227-254
16 Lindquist S. 1986. The heat-shock response. Annu. Rev. Biochem., 55: 1151-1191   DOI   ScienceOn
17 Pena FJ, Dominguez JC, Carbajo M, Anel L and Alegre B, 1998. Treatment of swine summer infertility syndrome by means of oxytocin under field conditions. Theriogenology, 49(4):829-836   DOI   ScienceOn
18 Rokutan K. 2000. Gastric mucosal protection and cell proliferation-role of heat shock proteins in gastric mucosal protection. Journal of Gastroenterology and Hepatology, 15: 12-19   DOI   ScienceOn
19 Shoichi S, Kaoru N, Makoto U and Hiroshi D. 1999. An additional exon of stress-inducible heat shock protein 70 Gene (HSP70-1). Biochemical and Biophysical Research Communications, 257: 193-198   DOI   ScienceOn
20 Vandemark NL and Free MJ. 1970. Temperature effects. In: Johnson AD, Gomes WR, Vandemark NL. (Eds.), The Testes, Vol. 3. Academic Press, New York, USA, pp. 233-312
21 Sarnbrook J, Fritsh EF and Maniatis T. 1989. Molecular Cloning: A Laboratory Manual (2nd Ed.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
22 Kuo YH, Huang SY and Lee YP. 1997. Effects of breed and season on semen characteristics of boars in subtropical area. J. Chin. Soc. Vet. Sci. 23: 114-122 (In Chinese, with English abstract)
23 Wertemann RP and Desjardins C. 1979. Testicular function in boars exposed to elevated ambient temperature. Biol, Reprod., 20: 235-241   DOI   ScienceOn
24 Cheng SP and Wung SC. 1974. A study of seasonal changes in the boar semen characteristics. Research Report Animal Industry Research Institute, Taiwan Sugar Corporation, pp. 89-103 (In Chinese, with English abstract)
25 Lindquist S and Craig EA. 1988. The heat-shock proteins. Annu. Rev. Genet., 22: 631-677   DOI   ScienceOn
26 Sanchez Y and Lindquist SL. 1990. HSPI04 is required for induced thermotolerance. Science, 248: 1112-1115   DOI
27 Koh TJ, Crabo BG, Tsou HL and Graham EF. 1976. Fertility of liquid boar semen as influenced by breed and season. J. Anim. Sci., 42: 138-144   DOI   PUBMED
28 Huang SY, Kuo YH, Lee YP, Tsou HL, Lin EC, Ju CC and Lee WC. 2000. Association of heat shock protein 70 with semen quality in boars. Anim. Reprod. Sci., 63: 231-240   DOI   ScienceOn
29 Christenson RK, Teaque HS, Grifo Jr. AP and Roller WL. 1972. The effect of high environmental temperature on the boar. Ohio Swine Research and Information Report, Ohio Agricultural Research and Development Center, Wooster, OH, USA, pp. 19-23
30 Malmgren L and Larsson K. 1984. Semen quality and fertility after heat stress in boars. Acta. Vet. Scand., 25: 425-435   PUBMED
31 Liu SH, Kuo YH, Yang TS and Lee KH. 1994. Relationship between characteristics of serum reproductive hormones and reproductive performance in Yorkshire boars during hot summer months. J. Chin. Soc. Anim. Sci. 23: 33-42 (In Chinese, with English abstract)
32 Craig EA and Gross CA. 1991. Is HSP70 the cellular thermometer? TIBS., 16: 135-140   DOI   PUBMED   ScienceOn
33 Richter LE, Romeny KF, Weitze KF and Zimmermann F. 1975. Zur Tiefgefrierung von Ebersperma. 7 Mitteilung: Weitere Labor-und Besamungsversuhe mit dem Verdunner Hulsenberg Ⅶ. Dtsch Tierarztl. Wschr., 82(4): 155-162
34 Shichijo K, Ihara M, Matsuu M, Ito M, Okumura Y and Sekine I. 2003. Overexpression of heat hsock protein 70 in stomach of stress-induced gastric ulcer-resistant rats. Digestive Disease and Science, 48: 340-348   DOI   ScienceOn
35 Sarge KD. 1995. Male germ cell-specific alteration in temperature set point of the cellular stress response. J. Biol. Chem., 270: 18745-18748   DOI