• Journal of the Korean Society for Precision Engineering
• /
• v.27 no.5
• /
• pp.85-91
• /
• 2010

In this paper, we present details on fabrication of single-cell electroporation microdevice, practical experiments of single-cell electroporation with our fabricated microdevice. Also, the continuous electroporation for the continuous flow of cells is used for high-throughput electroporation. The delivery efficiency and cell viability tests are provided and the successful GFP transfection into cells is also evaluated with a fluorescent microscope after electroporation. This device enables to reduce the size of samples and thus the use of small amount of reagents. Also, it makes it possible to permit to avoid cell discrimination (transfected cells versus non-transfected cells) encountered when traditional bulk electroporation is held.

• The Korean Journal of Applied Statistics
• /
• v.21 no.6
• /
• pp.1007-1014
• /
• 2008

Usability evaluation(UE) experiments are conducted to provide UE practitioners with guidelines for better outcomes. In UE research, significant quantities of empirical results have been accumulated in the past decades. While those results have been anticipated to integrate for producing generalized guidelines, traditional meta-analysis has limitations to combine UE empirical results that often show considerable heterogeneity. In this study, a new data mining method called weighted bi-clustering(WBC) was proposed to partition heterogeneous studies into homogeneous subsets. We applied the WBC to UE empirical results and identified two homogeneous subsets, each of which can be meta-analyzed. In addition, interactions between experimental conditions and UE methods were hypothesized based on the resulting partition and some interactions were confirmed via statistical tests.

• Korean Journal of Pharmacognosy
• /
• v.32 no.3 s.126
• /
• pp.238-241
• /
• 2001

The leaves and flowers of Hemisteptia lyrata Bunge (Compositae) have been used for cure sour throat and treat tumors in traditional chinese medicine. As a result of the chemical investigation, two compounds were isolated from the $CHCl_3$ extract of the flowers of H. lyrata. On the basis of spectroscopic experiments, their structures were identified as 8-hydroxy-3-keto-10(14)-guaien-12,6-oxide (Isoamberboin) and 3,8-dihydroxy-4(15),10(14),11(13)-guaiatrien-12,6-oxide (8-Hydroxyzaluzanin C). They were first isolated from the genus Hemisteptia.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.10 no.7
• /
• pp.3026-3049
• /
• 2016

Cloud computing promises high performance and cost-efficiency. However, most cloud infrastructures operate at a low utilization, which greatly adheres cost effectiveness. Previous works focus on seeking efficient virtual machine (VM) consolidation strategies to increase the utilization of virtual resources in production environment, but overlook the under-utilization of backup virtual resources. We propose a heuristic time sharing policy of backup VMs derived from the restless multi-armed bandit problem. The proposed policy achieves increasing backup virtual resources utilization and providing high availability. Both the results in simulation and prototype system experiments show that the traditional 1:1 backup provision can be extended to 1:M (M≫1) between the backup VMs and the service VMs, and the utilization of backup VMs can be enhanced significantly.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2002.10a
• /
• pp.383-386
• /
• 2002

It is very important to inspect color of printed texture in the textile process. To distinguish the color of the printed texture, RGB color values obtained from a scanner must be transformed to the standard colorimetric system used in the textile industry. It is XYZ color system that is defined by CIE(Commission Internationale do 1Eclairage). The mapping from RGB to XYZ color values is not simple and the scanner has even a positional deviation of RGB colors. In this paper an automatic color inspection method using a general scanning machine is presented. We used a U(neural network) model to map RGB to XYZ and compensate the positional error. In the real experiments, this inspection system shows to get very exact XYZ values from the traditional scanner regardless of the measuring position.

• Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
• /
• 2011.10a
• /
• pp.345-357
• /
• 2011

Recently increasing eco-efficiency of natural dyeing has become an important topic. The purpose of this study was to manufacture colored Wall paper for interior materials by combining color therapy and natural dyeing. For this purpose, through experiments with water-bleed, K/S value and chroma changes of samples in Wall paper for interior materials.

• Proceedings of the Korean Vacuum Society Conference
• /
• 2012.02a
• /
• pp.179-179
• /
• 2012

Low pressure radio-frequency glow discharges are investigated using theoretical modeling and various experimental diagnostic methods. In the calculations, global models and transformer models are developed to understand the chemical kinetics as well as the electrical properties such as the effective collision frequency, the heating mechanism and the power transferred to the plasma electrons. In addition, Boltzmann equation solver is used to compensate the effect of the electron energy distribution function (EEDF) shape in the global model, and the general expression of energy balance for non-Maxwellian electrons is developed. In the experiments, a number of traditional plasma diagnostic methods are used to compare with calculated results such as Langmuir probe, optical emission spectroscopy (OES), optical absorption spectroscopy (OAS) and two-photon absorption laser-induced fluorescence (TALIF). These theoretical and experimental methods are applied to understand several interesting phenomena in low pressure ICP discharges. The chemical and physical properties of low pressure ICP discharges are described and the applications of these methods are discussed.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.6 no.9
• /
• pp.2341-2350
• /
• 2012

Face detection is the first step of vision-based driver fatigue detection method. Traditional face detection methods have problems of high false-detection rates and long detection times. A space-time restrained Adaboost method is presented in this paper that resolves these problems. Firstly, the possible position of a driver's face in a video frame is measured relative to the previous frame. Secondly, a space-time restriction strategy is designed to restrain the detection window and scale of the Adaboost method to reduce time consumption and false-detection of face detection. Finally, a face knowledge restriction strategy is designed to confirm that the faces detected by this Adaboost method. Experiments compare the methods and confirm that a driver's face can be detected rapidly and precisely.

• Journal of Information Processing Systems
• /
• v.16 no.5
• /
• pp.1105-1112
• /
• 2020

Gait recognition, as a promising biometric, can be used in video-based surveillance and other security systems. However, due to the complexity of leg movement and the difference of external sampling conditions, gait recognition still faces many problems to be addressed. In this paper, an improved convolutional neural network (CNN) based on Gabor filter is therefore proposed to achieve gait recognition. Firstly, a gait feature extraction layer based on Gabor filter is inserted into the traditional CNNs, which is used to extract gait features from gait silhouette images. Then, in the process of gait classification, using the output of CNN as input, we utilize metric learning techniques to calculate distance between two gaits and achieve gait classification by k-nearest neighbors classifiers. Finally, several experiments are conducted on two open-accessed gait datasets and demonstrate that our method reaches state-of-the-art performances in terms of correct recognition rate on the OULP and CASIA-B datasets.

• Speech Sciences
• /
• v.13 no.4
• /
• pp.89-105
• /
• 2006

This study examines the phonetic realizations of English word stress to identify the influence of experiment method on experiment results. Stimuli uttered by native and Korean ESL beginners in authentic conversations are extracted to be shuffled according to their positions in utterances and information structure. Results indicate that the acoustic characteristics of English word stress are realized depending on its position in utterances. The native speakers correlate the stressed syllables in shorter duration with higher pitch and stronger intensity at sentence-final positions unlike the previous experiments and the traditional definition that stressed syllables are uttered in longer duration with higher pitch and stronger intensity; at sentence-medial positions, the native speakers correlate the stressed syllables in longer duration with higher pitch and no regularity in intensity or in shorter duration with lower pitch and intensity depending on their conversational intention. Korean ESL beginners correlate the stressed syllables in shorter duration regardless of positions in sentences with no regularity in pitch and intensity. This study, thus, shows that a different experiment method may result in different results on the phonetic realizations of English word stress.