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Single-cell Electroporation and Gene Transfection using MEMS-based Microdevice with Cantilever-type Microelectrode  

Cho, Young-Hak (School of Mechanical Design & Automation Engineering, Seoul Natl. Univ. of Tech.)
Kim, Beom-Joon (Precision Mechanical Engineering, the Univ. of Tokyo)
Publication Information
Journal of the Korean Society for Precision Engineering / v.27, no.5, 2010 , pp. 85-91 More about this Journal
Abstract
In this paper, we present details on fabrication of single-cell electroporation microdevice, practical experiments of single-cell electroporation with our fabricated microdevice. Also, the continuous electroporation for the continuous flow of cells is used for high-throughput electroporation. The delivery efficiency and cell viability tests are provided and the successful GFP transfection into cells is also evaluated with a fluorescent microscope after electroporation. This device enables to reduce the size of samples and thus the use of small amount of reagents. Also, it makes it possible to permit to avoid cell discrimination (transfected cells versus non-transfected cells) encountered when traditional bulk electroporation is held.
Keywords
Electroporation; Microcantilever-type Microelectrode; Green Fluorescent Protein(GFP); Gene Transfection;
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Times Cited By KSCI : 1  (Citation Analysis)
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