Journal of the Korean Society for Precision Engineering (한국정밀공학회지)

Korean Society for Precision Engineering (한국정밀공학회)
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Single-cell Electroporation and Gene Transfection using MEMS-based Microdevice with Cantilever-type Microelectrode

멤스 기반의 캔틸레버 형 전극을 가진 마이크로 디바이스를 이용한 단일세포의 Electroporation 및 유전자 Transfection

  • Cho, Young-Hak (School of Mechanical Design & Automation Engineering, Seoul Natl. Univ. of Tech.) ;
  • Kim, Beom-Joon (Precision Mechanical Engineering, the Univ. of Tokyo)
  • 조영학 (서울산업대학교 기계설계자동화공학부) ;
  • 김범준 (동경대학교 정밀기계공학과)
  • Received : 2010.02.04
  • Accepted : 2010.04.05
  • Published : 2010.05.01
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Abstract

In this paper, we present details on fabrication of single-cell electroporation microdevice, practical experiments of single-cell electroporation with our fabricated microdevice. Also, the continuous electroporation for the continuous flow of cells is used for high-throughput electroporation. The delivery efficiency and cell viability tests are provided and the successful GFP transfection into cells is also evaluated with a fluorescent microscope after electroporation. This device enables to reduce the size of samples and thus the use of small amount of reagents. Also, it makes it possible to permit to avoid cell discrimination (transfected cells versus non-transfected cells) encountered when traditional bulk electroporation is held.

Keywords

References

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