• Title/Summary/Keyword: total bacterial number

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Seasonal Variation of Total Bacterial Number and Environmental Factors in Daechung Reservoir (대청호에서 계절에 따른 세균 군집 및 환경요인의 변화)

  • Bing, Sun-Hye;Lee, Sang-Wook;Hwang, Soon-Jin;Oh, In-Hye
    • Korean Journal of Ecology and Environment
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    • v.35 no.2 s.98
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    • pp.71-78
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    • 2002
  • Seasonal change of total bacterial numbers were estimated in Daechung Reservoir from September, 1998 to August, 1999 and environmental factors which affects total bacterial numbers were estimated. Total bacterial numbers ranged between 1.2 and $19.0{\times}10^6\;cells/ml$ and averaged $6.9{\times}10^6\;cells/ml$. Bacterial numbers changed dynamically with season. The bacterial numbers were highest in July and showed peaks in April and July in sites studied except for the HOE (Hoenam site), In the HOE (Hoenam site), total bacterial numbers increased eve the period of March${\sim}$August. The correlation coefficient between bacterial number and chlorophyll a (<$200\;{\mu}m$) was r = 0.72(p< 0.05) at the MAN (man site). Total bacterial numbers in Daechung Reservoir was diversely changed by the seasonal variation of several environmental quality parameters.

Effects of ionized calcium on microbial cross-contamination in surface of carcass via slaughter process of chickens (이온화칼슘이 도계과정 중 도체표면의 미생물 교차오염에 미치는 영향)

  • Park, B.S.;Jin, J.Y.
    • Journal of the Korean Applied Science and Technology
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    • v.33 no.4
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    • pp.813-823
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    • 2016
  • The purpose of this study was to investigate the effects of ionized calcium treatment on total bacterial cross-contamination of chicken carcass surface in the slaughtering process. The growth of Escherichia coli was strongly inhibited in a medium prepared by using a 0.5% ionized calcium solution. The total bacterial cross-contamination of chicken carcass surface and the scalding water was significantly increased as the number of scalding was increased (p<0.05). The total bacterial cross-contamination of chicken carcass surface reached a plateau without a further increase as scalding was performed consecutively for 10 or more times. The total bacterial cross-contamination of the scalding water was significantly increased as the number of scalding was increased (p<0.05). The total bacterial cross-contamination of chicken carcass surface of the chickens raised on a floor type farm was significantly higher than that of the chickens raised in a battery cages (p<0.05). The total bacterial cross-contamination of chicken carcass surface of the chickens raised on a floor type farm was significantly lower in the 0.5% ionized calcium solution treatment group than in the control group (p<0.05).

Distribution and Dynamics of the Total Bacterial Number in the Kyongan Stream and Paltang Reservoir (경안천과 팔당호에서 총세균수의 분포 및 동태)

  • Park, Kyung-Mi;Hwang, Soon-Jin;Cho, Kyung-Je;Shin, Jae-Ki
    • Korean Journal of Ecology and Environment
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    • v.34 no.2 s.94
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    • pp.119-125
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    • 2001
  • Total bacterial density was investigated in the main stream and tributaries of the Kyongan Stream and inlet parts of Paltang Reservoir from September 2000 to February 2001 by acridine orange direct count (AODC) method. Total bacterial number in the Kyongan Stream was mainly under influence of the effluent discharge of sewage wastewater treatment plant (SWTP) located in the upstream or downstream. Decreasing rate with water flowing distance (km) in the main stream is $0.13\;{\time}\;10^6$ cells/ml, and it was estimated to much accumulating quantity on the stream bed during transport to downstream. Average values of total bacterial number in September${\sim}$October, November and December${\sim}$February were range $1.74{\sim}3.10{\time}10^6$, $1.86{\sim}7.30{\time}10^6$ and $4.56{\sim}8.75{\time}10^6$cells/ml, respectively, and were high at low temperature than that of high temperature period. Total bacterial number was more abundant at below $10^{\circ}C$ with $2.1{\sim}3.0$ folds than at above $10^{\circ}C$. Water quality by total bacterial number was classify to eutrophic and the potential of wastewater treated effluent for the microbial contamination assessed to very high. The results of this study indicate that the management of point source, SWTP effluent, is urgent to mitigate bacterial impact of Paltang Reservoir as well as the Kyongan Stream.

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Distribution and Activity of Heterotrophic Bacterial Communities in Kyeonggi Bay, Korea (경기만의 종속영양 세균군집의 분포 및 활성)

  • 강찬수;이기승;김명운;권개경;김용학;박성주;이건형;김상종
    • Korean Journal of Microbiology
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    • v.28 no.4
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    • pp.324-330
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    • 1990
  • The distribution of physicochemical environmental factors and microbiological factors was studied at 6 sampling sites in Kyeongge Bay of Yellow Sea from October 1989 to October 1990. The total bacterial number, saprophytic bacterial number, petroleum-degrading bacterial number, bacterial biomass, and bacterial secondary production were measured in the range of 0.09~1.24*10$^{7}$ cells/ml, 7~60000 CFUs/ml, 0~240 cells/ml, 14.16~301 .$\mu$g-C/l, and 0.13~11.82 mg-C/m$^{3}$/hr, respectively. The turnover times of $^{3}$H-glucose and $^{3}$H-acetate were in range of 6.5~6984 and 41~24897 hours, respectively. The spatial distribution of heterotrophic bacterial communities were hightly affected by influx of organic pollutants from the coastal area and the seawater exchange with offshore.

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Distribution of bacterial biomass in the water column of Soyang lake (소양호 수중 생태계에서의 세균 생체물질량의 분포)

  • 김명운;강찬수;김상종
    • Korean Journal of Microbiology
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    • v.27 no.2
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    • pp.130-138
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    • 1989
  • Microbiological parameters such as bacterial biovolume and biomass in Soyang Reservoir was statistically analyzed with the physico-chemical enviromental factors. Analysis of correlation and multiple regression showed that temperature affects most of microbiological parameters. Variations of total bacterial number, total bacterial biovolume and saprophyte number were highly correlatd with the concentrations of chlorophyll a and pheophytin a. Bacterial production by the $^{3}H$-thymidine incorporation rate was largely affected by Seston. It suggests that microbiological factors such as bacterial biovolume and bacterial biomass were controled by the concentration of seston and distribution of phytoplankton which acts as carbon and energy source for the bacterial community in the water column of Soyang Reservoir.

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Vertical Variation of Total Bacterial number in Daechung Reservoir (대청호에서 세균의 수직적 분포에 관한 연구)

  • Bing, Sun-Hye;Oh, In-Hye
    • The Journal of Natural Sciences
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    • v.16 no.1
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    • pp.143-154
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    • 2005
  • To define the ecological roles of bacterial community in Daechung Reservoir. Vertical variation of total bacterial numbers were estimated at MAN site for one year with DAPI staining. The bacterial numbers were counted, environmental factors were monitored at the surface, -5m, -10m, -15m and -25 m of MAN sited and tried to explain the vertical bacterial numbers with environmental factors. The bacterial number was $1.6-1.7.0 x10^6$ cells/ml at the surface, $2.3-11.0x10^6$ cells/ml at 5 m depth, $1.2-1.4.0x10^6$ cells/ml at 10 m depth, $1.4-15.0x10^6$ cells/ml at 15 m depth and $1.4-1.3.0x10^6$ cells/ml at 25 m depth. The Mean bacterial number at the surface was more that those at any other depth. The explanation of the vertical total bacterial numbers with environmental factors were suggested.

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The Activity and Structure of Bacterial Community within Artificial Vegetation Island (AVI) (인공 수초재배섬에서 세균의 활성과 세균 군집 구조)

  • Jeon, Nam-Hui;Park, Hae-Kyung;Byeon, Myeong-Seop;Choi, Myung-Jae
    • Journal of Korean Society on Water Environment
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    • v.23 no.5
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    • pp.676-682
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    • 2007
  • The bacterial number, extracellular enzyme activities and structure of bacterial community which are major constituent of aquatic ecosystem within the artificial vegetation island (AVI) were compared to those of the nearby pelagic lake waters in order to evaluate the possibility of the AVI as a eco-technological measure for water quality improvement and restoration of littoral zone in man-made reservoirs. There was not a significant difference in the total number of bacteria, but the number of active (viable) bacteria within the AVI was about 0.7 to 4.1 times higher than nearby pelagic lake water. The ratio of the number of active bacteria versus the total number of bacteria was also higher in the AVI than nearby pelagic lake water. The activities of ${\beta}$-glucosidase and phosphatase were 1.0 to 13.1 and 0.8 to 7.3 times higher respectively in the AVI than nearby pelagic lake water, showing that microorganisms were more active within the AVI. The bacterial communities of the two waters, examined by FISH method, did not indicate a clear difference in the springtime when the growth of macrophytes was immature, but during summer and fall it showed a clear difference indicating the formation of distinct bacterial community within the AVI compared to nearby lake water. From the results of this study, we conclude that AVI can contribute to make up the littoral ecosystem which show rapid cycling of matters through active detritus food chain in the dam reservoirs which have unstable aquatic ecosystem due to short hydraulic residence time and to strengthen the self-purification capacity of the lake.

Major Microbial Composition and Its Correlation to the Taste of Sunchang Traditional Kochujang (순창 전통 고추장의 주요 미생물 조성 및 맛과의 상관성)

  • Jin, Hyo-Sang;Kim, Jong-Bum;Lee, Kyung-Ja
    • The Korean Journal of Food And Nutrition
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    • v.20 no.4
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    • pp.363-368
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    • 2007
  • Traditional kochujang samples were collected from the folk village in Sunchang to find the major microbial composition and correlation between the taste and the microbial properties. Among the 29 samples, 17 samples showed Bacillus licheniformis as dominant strain with 11 samples Bacillus subtilis and 1 sample Staphylococcus pasteuri. Subdominant strain of 17 samples was Bacillus licheniformis with 12 samples Bacillus subtilis. Dominant strain numbers varied in rage of $6.60{\sim}8.38$ logCFU/g with subdominant strain number $5.90{\sim}7.86$ logCFU/g and total microbial number $6.64{\sim}8.56$ logCFU/g respectively. Bacterial type number varied in range of $6{\sim}18$ with the average 10.5 types. Fungi were found only in 2 samples and identified all to be Aspergillus oryzae. Yeasts were found in 25 samples. The dominant yeast strain of 23 samples was identified to be Zygosaccharomyces pseudorouxii and that of 2 samples Zygosaccharomyces rouxii. Yeast type variety showed that only single type was found in 15 samples, 2 types in 8 samples, 3 types in 1 sample and 4 types in 1 sample. Fungal number was 3.90 and 4.08 logCFU/g and yeast numbers varied in range of $3.90{\sim}6.43$ logCFU/g. The sensory scores of kochujang showed positive correlation with type numbers, but no significant correlation with the other microbial properties like total bacterial number, dominant bacterial number, subdominant bacterial number and dominant yeast number.

Antibiotic sensitivity and resistance of bacteria from odontogenic maxillofacial abscesses

  • Kang, Sang-Hoon;Kim, Moon-Key
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.45 no.6
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    • pp.324-331
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    • 2019
  • Objectives: This study investigated the types and antibiotic sensitivity of bacteria in odontogenic abscesses. Materials and Methods: Pus specimens from 1,772 patients were collected from affected areas during incision and drainage, and bacterial cultures and antibiotic sensitivity tests were performed. The number of antibiotic-resistant bacteria was analyzed relative to the total number of bacteria that were tested for antibiotic susceptibility. Results: Bacterial cultures from 1,772 patients showed a total of 2,489 bacterial species, 2,101 gram-positive and 388 gram-negative. For penicillin G susceptibility tests, 2 out of 31 Staphylococcus aureus strains tested showed sensitivity and 29 showed resistance. For ampicillin susceptibility tests, all 11 S. aureus strains tested showed resistance. In ampicillin susceptibility tests, 46 out of 50 Klebsiella pneumoniae subsp. pneumoniae strains tested showed resistance. Conclusion: When treating odontogenic maxillofacial abscesses, it is appropriate to use antibiotics other than penicillin G and ampicillin as the first-line treatment.

Analysis of Attached Bacterial Community of Biological Activated Carbon Process Using FISH (FISH 기법을 이용한 생물활성탄 공정에서의 운전기간별 부착 박테리아 군집변화 분석)

  • Son, Hyeng-Sik;Son, Hee-Jong;Park, Geun-Tae;Lee, Sang-Joon
    • Journal of Environmental Science International
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    • v.22 no.1
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    • pp.25-35
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    • 2013
  • The concentration of organic compounds was analyzed at each step of BAC process though $BDOC_{total/rapid/slow}$. Further, bacteria communities and biomass concentrations measured FISH and ATP methods were analyzed. The bed volume (BV) of steady state is different from that of based on assessment of organic compounds removal. Bed volumes in DOC, $BDOC_{rapid}$ and $BDOC_{total/slow}$ removal at steady state were around 27,500 (185.8 day), 15,000 (101.4 day) and 32,000 (216.2 day), respectively. A biomass didn't change after the bed volume reached 22,500 (152.0 day) according to analyzing ATP concentration of bacteria. The concentration of ATP was 2.14 ${\mu}g/g$ in BV 22,500 (152.0 day). The total bacterial number was $4.01{\pm}0.4{\times}10^7$ cells/g at the bed volume 1,150 (7.8 day) (the initial operation) and the number of bacteria was $9.27{\pm}0.2{\times}10^9$ at the bed volume 58,560 395.7 day) that increased more than 200 times. Bacterial uptrend was reduced and bacterial communities were stabilized since BV 18,720 (126.5 day). When BV were 1,150 (7.8 day), 8,916 (60.2 day), 18,720 (126.5 day), 31,005 (209.5 day), 49,632 (335.3 day), 58,560 (395.7 day), a proportion of total bacteria for the Eubacteria were 60.1%, 66.0%, 78.4%, 82.0%, 81.3% respectively. ${\gamma}$-Proteobacteria group was the most population throughout the entire range. The correlation coefficient ($r^2$) between Eubacteria biomass and ATP concentration was 0.9448.