• Journal of the Korea Organic Resources Recycling Association
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• v.18 no.2
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• pp.76-83
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• 2010

We(Hansol EME Co. Ltd.) proceeded anaerobic digestion test for domestic food wastewater applying to two operating method for increasing of OLR. The methods are as follows. One was the rapidity operating method which was increasing the OLR continuously and rapidly and the other was the terraced operating method which was increasing the OLR having adaptation period for each step. As a result of this tests, the ratio of VFA/Alkalinity of the process was very unstable under the rapidity operating method then the volume of produced biogas was dramatically decreased. However the process was shown stable performance under the terraced operating method maintaining the ratio of VFA/Alkalinity less than 0.4. Also, the process was performing the biogas recovery of $0.8Nm^3/kgVS_{rem}/d$ and the VS removal ratio of 85%. T-RFLP analysis about the community of bacteria and methanogen is also conducted to check the change of the microbial community according to the methods of OLR increasing operation. The microbial community was changed by the methods of OLR increasing operation according to the result of T-RFLP analysis. Although the anaerobic digestion test was executed by same pilot plant, the reactivity and the tolerance of microbial community for surrounding environment could be considerably changed by the operating method for the process.

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.3
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• pp.430-435
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• 2012

This study was carried out to investigate the rheological effects of black garlic extracts on the bread dough. 0, 5, 10 and 15% of black garlic extracts were added in the bread flour. Falling number, RVA (Rapid visco analyser), farinograph and alveograph were analyzed. Falling number was lowered by increasing the amount of the extract added in the wheat flour. The pasting characteristics of the dough by RVA, value of pasting temperature, peak viscosity, holding strength, final viscosity, break down and set back were increased compared to the control. In the Farinograph analysis, the consistency and the water absorption ratio were elevated with the increase of the extract added. However, the rapid decrease of stability and the time to breakdown values were observed by increasing the extract addition amount compared to the control. However the mixing tolerance index (MTI) was rapidly increased as the amount of the extract addition was increased, and the highest farinogram quality number was observed in the control. Through the alveograph analysis, $P_{max}$ value was found to show the highest value in the control, while the L and G values were higher but W values was lowered in the dough with added 15% black garlic extract. As a result of the analysis, there was no significant bad effect until 5% of black garlic extracts was added to the bread dough.

• Journal of Plant Biotechnology
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• v.44 no.3
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• pp.264-270
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• 2017

Brassinosteroid (BR), a plant steroid hormone, plays a critical role in the growth and developmental processes through its canonical signaling and crosstalk with various internal and external signaling pathways. Recent studies have revealed the essential interplay mechanisms between BR and ABA during seed germination and early seedling establishment. However, molecular mechanisms for this important signaling crosstalk are largely unknown. To understand the crosstalk between BR-mediated signaling pathways and ABA functions during early seedling development, we carried out a comparative genome-wide transcriptome analysis with an Agilent Arabidopsis $4{\times}44K$ oligo chip. We selected and compared the expression patterns of ABA response genes in ABA-insensitive bes1-D mutant with wild type seedlings on which ABA was exogenously applied. As a result, we identified 2,353 significant differentially expressed genes (DEGs) in ABA-treated bes1-D and wild type seedlings. GO enrichment analysis revealed that ABA signaling, response, and metabolism were critically down-regulated by BR-activated signaling pathways. In addition, the genome-wide transcriptome analysis data revealed that BR-regulated signaling pathways were tightly connected to diverse signal cues including abiotic/biotic stress, auxin, ROS etc. In this study, we newly identified the molecular mechanisms of BR-mediated repression of ABA signaling outputs. Also, our data suggest that interplay among diverse signaling pathways is critical for the adaptive response of the plant to various environmental factors.

• Molecules and Cells
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• v.39 no.2
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• pp.111-118
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• 2016

MiR399f plays a crucial role in maintaining phosphate homeostasis in Arabidopsis thaliana. Under phosphate starvation conditions, AtMYB2, which plays a role in plant salt and drought stress responses, directly regulates the expression of miR399f. In this study, we found that miR399f also participates in plant responses to abscisic acid (ABA), and to abiotic stresses including salt and drought. Salt and ABA treatment induced the expression of miR399f, as confirmed by histochemical analysis of promoter-GUS fusions. Transgenic Arabidopsis plants overexpressing miR399f (miR399f-OE) exhibited enhanced tolerance to salt stress and exogenous ABA, but hypersensitivity to drought. Our in silico analysis identified ABF3 and CSP41b as putative target genes of miR399f, and expression analysis revealed that mRNA levels of ABF3 and CSP41b decreased remarkably in miR399f-OE plants under salt stress and in response to treatment with ABA. Moreover, we showed that activation of stress-responsive gene expression in response to salt stress and ABA treatment was impaired in miR399f-OE plants. Thus, these results suggested that in addition to phosphate starvation signaling, miR399f might also modulates plant responses to salt, ABA, and drought, by regulating the expression of newly discovered target genes such as ABF3 and CSP41b.

• Molecules and Cells
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• v.28 no.4
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• pp.383-388
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• 2009

The dehydration responsive element binding protein 2C (DREB2C) is a dehydration responsive element/C-repeat (DRE/CRT)-motif binding transcription factor that induced by mild heat stress. Previous experiments established that overexpression of DREB2C cDNA driven by the cauliflower mosaic virus 35S promoter (35S:DREB2C) resulted in increased heat tolerance in Arabidopsis. We first analyzed the proteomic profiles in wild-type and 35S:DREB2C plants at a normal temperature ($22^{\circ}C$), but could not detect any differences between the proteomes of wild-type and 35S: DREB2C plants. The transcript level of DREB2C in 35S: DREB2C plants after treatment with mild heat stress was increased more than two times compared with expression in 35S:DREB2C plants under unstressed condition. A proteomic approach was used to decipher the molecular mechanisms underlying thermotolerance in 35S:DREB2C Arabidopsis plants. Eleven protein spots were identified as being differentially regulated in 35S:DREB2C plants. Moreover, in silico motif analysis showed that peptidyl-prolyl isomerase ROC4, glutathione transferase 8, pyridoxal biosynthesis protein PDX1, and elongation factor Tu contained one or more DRE/CRT motifs. To our knowledge, this study is the first to identify possible targets of DREB2C transcription factors at the protein level. The proteomic results were in agreement with transcriptional data.

• Korean Journal of Ecology and Environment
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• v.43 no.1
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• pp.69-81
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• 2010

This study was conducted at 25 sites of 6 mainstreams and 19 tributaries sites within Heuck Stream watershed during May~October 2003 for the survey of fish distributions, compositions, and community characteristics. The survey showed that total fish was identified as 9 family and 26 species in all sites, and Cyprinidae dominated the community as 15 species. The dominant species of >20% of the total were Zacco temminckii (29%), Zacco platypus (22%), and Rhynchocypris oxycephalus (21%) in the watershed. Community analysis, based on the stream spatial gradients, indicated composition differences along the main axis of the stream from the headwater to the downstream; R. oxycephalus predominated in the most headwater zone and Z. temminckii dominated in the lower headwater zone, whereas Z. temminckii -Z. platypus dominated in the mid-to-downstream, and Z. platypus dominated the community in the most downstream zone. Total endemic species was 7 family and 15 species, which is made of 50% in the fish community, so that the high proportion of endemic species indicated a healthy region in terms of fish community, compared to average 23% in Korean peninsula in general. The total number and species of fish increased as the stream order (stream size) increased, indicating that impacts on chemical pollution or habitat disturbance were not so large to the fish community in this watershed. Trophic and tolerance guilds analysis showed that relative proportions of sensitive and insectivore species were >50% in the watershed and decreased as the stream order increased, whereas relative proportions of tolerant and omnivore species showed an opposite results. These outcomes suggest that the natural condition of watershed is preserved relatively and the region should be protected from the chemical and habitat disturbace by agricultural activity and urban developments.

• Korean Journal of Environment and Ecology
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• v.29 no.6
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• pp.873-883
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• 2015

From August 2012 to April 2013, the ichthyofauna and post-restoration properties of Yangjae stream were investigated. A total of 1,503 fish of 2 forms, 16 species and 5 families were studied during the survey period. A survey of the distribution of freshwater fish inhabiting Yangjae stream after restoration of the stream showed large populations of Zacco platypus and Rhynchocypris oxycephalus. From the frequency distribution of the total length for the Zacco platypus population inhabiting Yangjae stream, it was estimated that there are 4 distinct age groups, with an abundance of the 27-63 mm (Age $0^+$) category accounting for 51.3% of the population. The regression coefficient (b) for the length-weight relationship in Zacco platypus was 3.27, while the slope of the condition factor was positive, demonstrating that stable growth conditions are being maintained. Analysis of tolerance and trophic guilds showed a decrease in the numbers of sensitive species and insectivore species further downstream, and an increase in tolerant species and omnivore species. Principal component analysis using community indices and indices of biotic integrity (IBI) showed that there were differences according to the survey time: the first and second surveys showed major changes on certain single species populations while during the third survey, it was analyzed that the populations of various types of fish had established balance.

• Korean Journal of Environment and Ecology
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• v.26 no.1
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• pp.91-99
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• 2012

The purpose of this study was to find out the vegetation community structure and the distribution characteristics of Buxus koreana through analysis of the topography and soil characteristics in Kwanaksan Ecological and Scenery Conservation Area. To do this twenty two plots as $100m^2$ each were set up and investigated. As a result, the vegetation communities were classified in four groups; Pinus rigida community (I), P. rigida - B. koreana community(II), Quercus mongolica community(III), Quercus acutissima - Quercus serrata community(IV). The distribution characteristics in topography appeared that B. koreana distributed more in valley where soil water and nutrition conditions are good. The drainage condition also influenced the distribution, because the ratio of clay was low in the result of soil texture analysis. B. koreana was distributed in high-pitched slope and the high rock ratio area. According to the soil analysis, the mean soil pH of B. koreana distributed groups(pH 4.79) was higher than that of non-B. koreana distributed groups(pH 4.41). It appeared that B. koreana had tolerance to the barren soil, so the value of exchangeable cations such as $Ca^{2+}$ and $Mg^{2+}$were showed lower than that of the average of Korean forest soil.

• Korean Journal of Environmental Biology
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• v.31 no.4
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• pp.440-447
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• 2013

The ecological health, based on the Index of Biological Integrity (IBI) and Qualitative Habitat Evaluation Index (QHEI) was evaluated in 10 stream sites of Southern Han River. Eleven parameters of 12 parameters (Karr 1981) were modified for the application of regional Korean circumstance. The ecological health, based on IBI grade, was in "good condition" and the IBI score ranged from 33 to 47. Nine parameters of the original 12-parameter metrics in QHEI model (Plafkin et al. 1989) were applied in the habitat assessment. The mean QHEI model values were judged as "partially supporting" and ranged from 75 (non-supporting) to 109 (supporting). Comparative analyses revealed that values of IBI and QHEI models were greater in Gj stream than Ig- and Dn streams. The analysis of fish compositions showed that the proportions of insectivore, omnivore, and carnivore were 61.9%, 19%, and 9.5%, respectively. According to tolerance guild analysis, sensitive species and tolerant species were 76.1% and 4.7%, respectively, indicating a healthy trophic state in terms of food chain. The analysis by habitat guild type indicated that riffle benthic species dominated (57.1%) when compared to water column species (28.5%). The introduced species and individuals with diseases or external abnormality were not observed. Overall, the model values of IBI and QHEI suggested that the ecological health was maintained well in this upstream region.

• Korean Journal of Environmental Biology
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• v.35 no.4
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• pp.684-693
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• 2017

The purpose of this study was to analyze fish communities in the Wonju stream located in Wonju-si in Gangwon-do, and to evaluate the health of the stream through past and present changes. We performed investigations four times from May 2015 to September 2016. In the survey, 5,201 individuals which belonged to 27 species and 9 families were collected. Zacco platypus was the dominant species and Pungtungia herziwas the subdominant species. Eight Korean endemic species (Acanthorhodeus gracilis, Zacco koreanus, Coreoleuciscus splendidus, Squalidus gracilis majimae, Microphysogobio yaluensis, Iksookimia koreensis, Koreocobitis rotundicaudata, Coreoperca herzi) were observed and showed a ratio of 16.54%. The fish community of dominance ($0.72{\pm}0.10$), diversity ($1.37{\pm}0.32$), evenness ($0.61{\pm}0.13$), and richness ($1.70{\pm}0.23$) were evaluated. According to our analysis of tolerance guilds, the total individual number of intermediate species was higher than the sensitive and tolerant species. As a result of the trophic guild analysis,the omnivore and insectivore species were relatively high. The value of the qualitative habitat evaluation index (QHEI) in the wonju stream was averaged 121.2 (${\pm}23.4$), indicating a suboptimal condition. Stream health showed that the fish assessment index (FAI) value was an A to C grade in the Wonju stream. A correlation coefficient analysis with FAI and various factors was analysed statistically, and had a high correlation in QHEI, with the omnivore species, insectivore species, intermediate species, sensitive species, dominance, diversity, evenness, and richness.