• KOREAN JOURNAL OF CROP SCIENCE
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• v.46 no.4
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• pp.279-283
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• 2001

The content and composition of tocopherol were analyzed in peanuts produced in Korea. The tocopherol assay was consisted of an extraction with n-hex-ane:isopropanol mixture followed by high pressure liquid chromatography with fluorescence detection. Tocopherol content was higher in leaves and seeds but lower in seed coats and shells. $\alpha$-/${\gamma}$- Tocopherol ratio was as low as 0.53 in seeds and as high as 22.5 in shells. Tocopherol content in seeds of nine varieties ranged from 142 to 220 ng/mg dry weight, and the $\alpha$-/${\gamma}$-tocopherol ratio from 0.40 to 0.75. Tocopherol content decreased by 18% but $\alpha$-/${\gamma}$-tocopherol ratio increased by 44% in roasted seeds. The results indicate that the level of tocopherol in Korean peanut varieties is moderate and the ratio of $\alpha$- to ${\gamma}$-tocopherol is low.

• Journal of Nutrition and Health
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• v.19 no.5
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• pp.304-314
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• 1986

To observe the effect of the different level of PUFA and marginal tocopherol supplement on HDL-chol, tissue tocopherol content and fatty acid composition, the rats were supplied either safflower oil or conconut oil with or without tocopherol supplement to the experimental diet. Plasma tocopherol level was not greatly influenced by the different dietary fat and similar effect was observed in the liver but not in the adipose tissue. HDL-chol level was reduced in the high PUFA diet regardless of tocopherol content. No effect by tocopherol supplement was observed in the fatty acid composition of liver and adipose tissue lipid in both dietary PUFA levels . There was also no increase in the content of tissue polyenoid acid by tocopherol in the high PUFA diet . Fatty acid composition of tissue lipid was rather more influenced by dietary fat. Lauric and myristic acid contents were higher in the low PUFA diet and linoleic acd and total polynoic acid content were higher in the high PUFA diet. With tocopherol supplement tocopherol /PUFA ratio of tissue was increased but the ratio of high PUFA diet was significantly lower than that of low PUFA diet. Marginal tocopherol supplement could not reduce the peroxidizability index of high PUFA diet.

• Molecules and Cells
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• v.24 no.2
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• pp.301-306
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• 2007

Tocopherols, essential components of the human diet, are synthesized exclusively by photosynthetic organisms. To increase tocopherol content by increasing total flux to the tocopherol biosynthetic pathway, genes encoding Arabidopsis homogentisate phytyltransferase (HPT/V-TE2) and tocopherol cyclase (TC/VTE1) were constitutively overexpressed in lettuce (Lactuca sativa L.). Total tocopherol content of the transgenic plants overexpressing either of the genes was increased by more than 2-fold mainly due to an increase in ${\gamma}$-tocopherol. However, chlorophyll content in the HPT/VTE2 and TC/VTE1 transgenic lines decreased by up to 20% and increased by up to 35%, respectively (P < 0.01). These results demonstrate that manipulation of the tocopherol biosynthetic pathway can increase or decrease chlorophyll content depending on the gene introduced.

• Journal of Nutrition and Health
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• v.23 no.2
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• pp.81-92
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• 1990

Ten college women were treated with either corn oil(CO< as a source of C18:2, $\omega$6) or fish oil concentrates(FO, as a source of C20:5+C22:6, $\omega$3) with experimental diet for 7 days and then returned to normal home made diet. Kinetic changes of plasma and RBC lipids, tocopherol, MDA levels and hemolysis were observed at day-0, -3, -7, -8, -10, -14 and -21 of experimental periods. Plasma and RBC tocopherol contents were significantly increased at day-3 and -7 by tocopherol tocopherol supplement in dietary oil, but there was no significant difference between CO and FO diet with large dose of tocopherol supplement. After stop experimental regimen at day-7, plasma tocopherol content was sustained at high level until day-10 but drastically decreased at day-14 and remained at low level at day-21. However, RBC tocopherol level was not greatly responded to the dietary intake of tocopherol and was varied in the very narrow range. MDa levels of plasma and RBC were not responded in the same way as tocopherol content of plasma. MDa content of RBC was very low compared to that of plasma. RBC hemolysis by incubation in hypotonic solution was negatively correlated to plasma tocopherol level and was not correlated to the level of MDa in either plasma or RBC. There were no significant change in the levels of plasma cholesterol, HDL-chol, triglyceride, phospholipid, and lipoprotein pattern throught experimental periods.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.57 no.1
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• pp.83-88
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• 2012

In order to get information on tocopherol content and composition in fifty-six soybean germplasms were evaluated by HPLC. From the principle component analysis, the first three components accounted for 71.6% of the total variance of tocopherol content in the germplasms. Principal component 1 showed significant correlations with all the morphological markers except 100-seed weight. Soybean germplasms were divided into three groups by the first two principal components. The highest content of ${\alpha}$-tocopherol was $38.6{\mu}g$ per g in Tanner, while that of ${\gamma}$-tocopherol was $195.6{\mu}g$ in PI 91073. In case of ${\delta}$-tocopherol, IT 105622 showed the highest value as $29.8{\mu}g$. The contents of tocopherol were gradually increased from the late August to late September, which was 10 days before maturity, in Alchankong and PI 96322. The tocopherol content was higher in seeds from the plants sown early than those sown late. Total tocopherol contents of PI 96188, Geomjeongkong 2, and Suwon 183 grown in Gimje were higher than those grown in Iksan, but the difference was not observed in PI 96322. These results suggest that the contents of tocopherol in soybean were affected not only by the genotypes but also by environment.

• Journal of Animal Science and Technology
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• v.48 no.2
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• pp.279-286
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• 2006

This study was carried out to investigate the effects of addition of milk minerals (2%) and α-tocopherol (0.03%) on lipid oxidation and minerals content of emulsion-type sausages. The pH values of sausage containing milk mineral alone and combined with milk mineral and α-tocopherol were significantly (P<0.05) higher than those for control and α-tocopherol. The POV values of sausage containing combined with milk mineral and α-tocopherol were significantly (P<0.05) lower than those of control and α-tocopherol during storage. The TBARS values of sausage containing milk mineral alone and combined with milk mineral and α-tocopherol were significantly (P<0.05) lower then those for control and α-tocopherol. The Mg, Ca and P content of sausage containing combined with milk mineral and α-tocopherol were significantly (P<0.05) higher than those of control and the other. The K content of sausage containing α-tocopherol were significantly (P<0.05) higher than those of control and the other. Milk mineral, when used with α-tocopherol, provided no additional effect on lipid oxidation, but mineral contents were higher than those of control.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.6
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• pp.468-471
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• 2004

Tocotrienol $(T_3)$ and tocopherol (T) are well-known antioxidant vitamin E isomers that are enriched in rice bran, most of which is removed during polishing process. To verify the polishing-dependent fractional content of tocotrienol and tocopherol, harvested brown rice of 4 cultivars were polished by 4.0, 6.4, 8.0, 9.6, and $12\%$ (w/w) and resultant bran powder and rice were used for $T_3$ and T analysis. Polishing degree affected bran $T_3$ content in that bran polished by $6.4\%$ exhibited highest $T_3$ content compared to other polishing degrees except in cv. Chucheongbyeo which showed similar $T_3$ content from 4.0 to $8.0\%$ polishing. In case of tocopherol, bran polished by $8.0\%$ exhibited highest T content in all tested cultivars. In general, $T_3$ distributed in higher quantity in exterior i.e., in less polished bran, while T showed relatively less decrease with enhanced polishing degree. Consequently, total vitamin E content in bran as calculated by the summation of $T_3$ and T became less dependent on polishing degree in that bran polished between 6.4 to $9.6\%$ exhibited no statistical differences. The rice of cv. Chuchongbyeo, remained after polishing, exhibited similar vitamin E content up to $6.4\%$ polishing, above which significant vit E loss could be observed. These results suggest that polishing less than $6.4\%$ is desirable to minimize the loss of tocotrienols and tocopherols during polishing.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.54 no.1
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• pp.80-87
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• 2009

Aiming at development of region specialized crop, this study was conducted to clarify variety and cultivation region dependent on antioxidative compounds in black soybean seeds. For this purpose two black soybean varieties (Ilpumgeomjeongkong and Cheongjakong) were cultivated in 3 different regions (Hwaseong in Gyeonggido, Naju in Jeollanamdo and Jinju in Gyeongsangnamdo) in 2004 and 2005, and harvested seeds were used for isoflavone, anthocyanin and tocopherol contents along with electron donating ability-based antioxidative activities measurements. 100 grain weight between two varieties were not significantly different, but Hwaseong district showed higher 100 grain weight compared to Jinju and Naju. Ilpumgeomjeongkong was higher total isoflavone content ($1,064.9{\mu}g/g$) compared to Cheongjakong ($801.3{\mu}g/g$) in 2004, whereas Cheongjakong showed higher in 2005 compared to Ilpumgeomjeongkong. The highest isoflavone content was obtained in Hwaseong district in 2004, whereas it is the reverse in 2005 that Jinju district showed the highest isoflavone content. In total anthocyanin content, Ilpumgeomjeongkong (7.22 mg/g) was higher than that of Cheongjakong (6.83 mg/g), and Jinju district showed the highest total anthocyanin content (9.16 mg/g) compared to Naju and Hwaseong cultivating districts in their three cultivating districts. Total tocopherol content showed no significant difference between two varieties, but Hwaseong ($217.2{\mu}g/g$) and Jinju ($216.3{\mu}g/g$) districts showed higher content compared to Naju ($189.7{\mu}g/g$) among three cultivating districts. In tocopherol content ratio, $\gamma$-tocopherol was the highest from 56.2% to 59.9%. In electron donating ability (EDA) between two cultivars, Ilpumgeomjeongkong was significantly higher than Cheongjakong, and Naju was the highest of 55.6% among three cultivating districts.

• KSBB Journal
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• v.20 no.3
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• pp.210-214
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• 2005

The characteristics of sesame oil containing one of natural antioxidant, ' $\gamma$-tocopherol', were studied with the supercritical $CO_2$ extraction. Although $\gamma$-tocopherol has a lower vitamin E value in biological systems than $\alpha$-tocopherol, it is a more potent antioxidant with in oils. For the research of various factors influence to the $\gamma$-tocopherol contents increment, we have checked roasting time and temperature, as well as pressure, temperature and flow rate of supercritical fluid. As a result, we found that the $\gamma$-tocopherol content was maintained constant under the condition of roasting temperature over $200^{\circ}C$. With the longer roasting time, $\gamma$-tocopherol content was increased. Except 250 bar, the $\gamma$-tocopherol content was maintained constant under the condition of the various pressure of supercritical fluid. But $\gamma$-tocopherol content was increased with lower flow rate of supercritical fluid from 1 $m{\ell}$/L to 3 $m{\ell}$/L. When the extraction performance with the supercritical fluid was compared to the conventional compressed extraction, $\gamma$-tocopherol content was increased up to 1.6 times.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.7
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• pp.1071-1076
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• 2005

Gamma irradiation treatment brings about biochemical changes that could affect nutritional Quality of food. This study was conducted to examine the content of $\beta-Carotene$ in Jeju orange, vitamin $D_3$ in anchovy and $\alpha-Tocopherol$ in beef. $\beta-Carotene$ content of Jeju orange was not affected by irradiation of 5 and 10kGy. In anchovy, vitamin $D_3$ content was dose-dependently increased to $109.30\%\;and\;125.25\%$ of original content by irradiation of 5 and 10kGy, respectively. $\alpha-Tocopherol$ content in beef with high fat was progressively reduced by $17\%$ of original value with irradiation of 1kGy and by $84\%$ with 10kGy. It could be concluded that $\alpha-tocopherol$ might be most vulnerable to irradiation, while vitamin $D_3$ is very stable against irradiation within 10kGy of gamma irradiation.