• Journal of Microbiology and Biotechnology
• /
• v.23 no.6
• /
• pp.771-778
• /
• 2013

Essential oils are increasingly of interest for use as novel drugs acting as antimicrobial and antiviral agents. In the present study, we report the in vitro antiviral activities of 29 essential oils, extracted from Chinese indigenous aromatic plants, against the tobacco mosaic virus (TMV). Of these essential oils, those oils from ginger, lemon, tea tree, tangerine peel, artemisia, and lemongrass effected a more than 50% inhibition of TMV at 100 ${\mu}g/ml$. In addition, the mode of antiviral action of the active essential oils was also determined. Essential oils isolated from artemisia and lemongrass possessed potent inactivation and curative effects in vivo and had a directly passivating effect on TMV infection in a dose-dependent manner. However, all other active essential oils exhibited a moderate protective effect in vivo. The chemical constitutions of the essential oils from ginger, lemon, tea tree, tangerine peel, artemisia, and lemongrass were identified by gas chromatography and gas chromatography-mass spectrometry. The major components of these essential oils were ${\alpha}$-zingiberene (35.21%), limonene (76.25%), terpinen-4-ol (41.20%), limonene (80.95%), 1,8-cineole (27.45%), and terpinolene (10.67%). The curative effects of 10 individual compounds from the active essential oils on TMV infection were also examined in vivo. The compounds from citronellal, limonene, 1,8-cineole, and ${\alpha}$-zingiberene effected a more than 40% inhibition rate for TMV infection, and the other compounds demonstrated moderate activities at 320 ${\mu}g/ml$ in vivo. There results indicate that the essential oils isolated from artemisia and lemongrass, and the individual compound citronellal, have the potential to be used as an effective alternative for the treatment of tobacco plants infected with TMV under greenhouse conditions.

• Journal of Applied Biological Chemistry
• /
• v.42 no.4
• /
• pp.161-165
• /
• 1999

The antiviral activity of CAP30 from Chenopodium album, a type1 ribosome-inactivating protein (RIP), was examined against 5 different plant viral pathogens, and its activity against Tobacco mosaic virus was compared to those of well known antiviral proteins such as Pokeweed Antiviral protein from leaves and seeds. When the inoculating concentration of Tobacco mosaic virus was varied from 0.4 to $400{\mu}g/ml$, it was observed that CAP30 at the concentration of $1{\mu}g/ml$ suppressed the viral infection of C. amaranthicolor and C. quinoa almost completely up to $40{\mu}g/ml$ Tobacco mosaic virus. Results from the assays for the inhibitions of in vitro translation of rabbit reticulocyte lysate and the suppression of Tobacco mosaic virus infection ($10{\mu}g/ml$) to C. quinoa indicated that CAP30 is a strong inhibitor of protein synthesis and virus infection. The infection of several viruses other than Tobacco mosaic virus to host plants were also inhibited by $5{\mu}g/ml$ CAP30, suggesting that a gene encoding CAP30 can be used to develop transgenic virus-resistant plants.

• Journal of the Korean Society of Tobacco Science
• /
• v.1 no.1
• /
• pp.21-32
• /
• 1979

This experiment was carried out to fond out the proper transplanting time of burley tobacco since the transplanting dates In Jeonla Do varied from early April to late June. In this experiment , the first transplanting was lone on April 10 and continued until June, 20 with the total of 7 Plantings. The results of experiment from 1977 to 1978 showed no significant differences on value per kg (won /kg ) from April 10 to May 10 whether the plants were grown with mulching or non -mulching The yield and price per 10a were highest with May, 10 transplanting . The proper transplanting date obtained by regression analysis, however, was between May, 1 to May 5.

• Journal of the Korean Society of Tobacco Science
• /
• v.16 no.2
• /
• pp.139-143
• /
• 1994

This study was carried out to control the stalk curing period in Burley Tobacco, for its period was longer than 40 days. The 5 methods were treated to shorten the stalk curing period. The curing period was shortened for 1~3 days by the harvesting methods and the amount of predehydration, but it was shortened for 8 days by the hanging methods(sloping and horizontal curing)and the hanging density(90 plants/3.3m2) without the decrease of quality. In the ventilating condition of the curing house, the curing period could be controlled for 5 dyes with the quality like the conventional cured leaves by closing the curing house from the late curing stage. But when the curing house was not ventilated earlier, however the curing period was shortened but its quality was decreased.

• Journal of the Korean Society of Tobacco Science
• /
• v.17 no.2
• /
• pp.114-119
• /
• 1995

Cultivars of Nicotiana tabacum L. normally have pink flowers, but the flue-cured tobacco mutant line, BU 8832-85, had white flower. The mutant line was crossed with five normal varieties of KF 109, NC 82, TC 499, NC 567 and Coker 176. All Fl plants showed pink flower. The progenies of F2 generations were segregated with the phenotypic ratio 9 : 3 : 4 with pink, varigated(a recombinant type) and white flower, respectively. Test-cross populations showed 1 : 1 : 2 ratios. These results showed that the white flower character was controlled by two recessive genes. The genes were designated as FFCC for pink and ffcc for white flower. The recessive gene ff was epistatic to C and c. Therefore, white flower had a recessive epistasis gene.

• Journal of the Korean Society of Tobacco Science
• /
• v.5 no.1
• /
• pp.3-9
• /
• 1983

Due to the wide and effective use of sucker controlling chemicals, gentic studies on the suckering habits of tobacco plants have long been neglected. So, to investigate the genetic basis of suckering, crosses between "Little Dutch" and "Cuban" were made, and subsequent generations, $F_1F_2,\;BC_1,\;BC_2,$ and parents were grown on the field. Several morphological characters were measured and the relation among them were analyzed. Correlation coefficients between leaf shape and sucker leaf shape, and the number of suckers and sucker weight were 0.25 and 0.42, both sighificant at 1 % level. But negative correlation between number of suckers per plant and that of leaves was observed, though statistically not significant. Dominance was observed in the number of suckers and amount of suckers produced, and the heritability of sucker shape was calculated as high as 0.88. And two paris of genes seemed to be involved in the inheritance of sucker shape, and narrow leaf appeared as dominant in the $F_1$.inant in the $F_1$.

• Journal of the Korean Society of Tobacco Science
• /
• v.15 no.2
• /
• pp.115-122
• /
• 1993

KB103 was developed from a cross between Burley 21 and Va 528 in 1983. It was developed by haploid method derived from anther culture method. It has moderate resistance to black shank(Phytophhorn nicotianae Breda de Haan Var. nictiana Waterhouse). KB103 produced good yields and quality in 1988-1990. It flowered about four or five days later than standard variety Burley 21, and plant height, leaf width, leaves per plants were similar to those of Burley 21. In maturing and curing properties as well as in chemical constituents of cured leaves, KB 103 was very similar to that of Burley 21. Regional farm test with the breeding line, KB 8304- ADH 65 named as KB 103 in 1986, was performed for 4 crop years from 1987 at 29 locations. It was released on burley tobacco area in 1992.

• The Plant Pathology Journal
• /
• v.20 no.4
• /
• pp.289-292
• /
• 2004

The population of Ralstonia solanacearum (Rs) in soil is very important as a primary inoculum source of bacterial wilt in tobacco fields. To investigate the population of Rs, physical properties and chemical components during the tobacco growing season, soil samples were taken from the fifteen fields which were located in the flue-cured tobacco growing area, Ansung, Kyunggi province and Wonju, Kangwon province. Two fields of the fifteen were bacterial wilt free. Six fields had less than 10% plants being diseased and seven over 10%. The Rs population level determined by using SMSA medium generally showed an up-and-down pattern being low in May, high in Jun and July and low in August. The soil population in May and June showed a positive correlation with the incidence of bacterial wilt (r=0.571$^*$, r=0.688$^{**}$), but P$_2O_5$, content of soil was negatively correlated with the disease incidence (r=-0.539$^*$). These results suggest that Rs population in soil examined in May or in June, and the P$_2O_5$ content in soil should be key factors to determine the bacterial wilt potential of tobacco fields.

• Journal of the Korean Society of Tobacco Science
• /
• v.22 no.2
• /
• pp.107-113
• /
• 2000

This study was carried out to investigate the changes in composition of the non-volatile organic acid, fatty acid and polyphenolic compounds during air-curing in burley tobacco leaves, and the effect of curing methods on the contents in air-cured leaves. The air-cured variety, (Nicotiana tabacum cv KB108) was normally grown at the Chonju tobacco experiment station in 1998. Plants designated for the each curing methods were harvested on the same date, and the ripe leaves for primed curing were harvested at the tenth leaf position from the top on the stalk. The major compounds of non-volatile organic acid and fatty acid were malic, citric, oxalic, palmitic, and linolenic acid. The concentrations of malic acid, unsaturated fatty acids, chlorogenic acid and rutin in cured leaves were remarkably decreased during curing, while citric acid was increased. The changes of these compounds showed the similar pattern during both primed and stalk curing. In connection with curing methods, the contents of malic, linoleic and linolenic acid were higher in excessive cured leaves than those in primed cured or stalk cured leaves, while the content of citric acid was lower in excessive cured leaves than that in primed cured or stalk cured leaves.

• Journal of the Korean Society of Tobacco Science
• /
• v.8 no.1
• /
• pp.49-56
• /
• 1986

To obtain the information of low chlorine leaf production, the chlorine content of the soil and cured leaf in Burley tobacco growing area was investigated. The results obtained are as follows. 1. The chlorine concentration in soil at transplanting time in Kwang-ju growing area was higher than in Jeon-ju(1983), and paddy field sell showed higher concentration of chlorine than upland field in Jeon-ju(1984). The chlorine concentration in soil was different between years, locations, and cultivated land (paddy field and upland field), but the concentration, on the whole, was under the levels that might effect the leaf quality. 2. The chlorine concentration In soil was highest around todays after transplanting, and after harvesting, the concentration decreased to half of transplanting time. 3. The distribution of chlorine through the parts of tobacco plant showed remarkable difference between years, locations, cultivated land and growing stages. 4. There was significant positive correlation between chlorine concentration in top soil and chlorine content of cured leaf. 5. Chlorine content of cured leaf in Kwang-ju area(1.79%) was higher than that of Jeon-ju area(O.79%), relatively. 6. It was considered that the accumulation of salt from the sea by sea. wind did not main factor of high leaf chlorine content in seaside district.