The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Relationship between the Population of Ralstonia solanacearum in Soil and the Incidence of Bacterial Wilt in the Naturally Infested Tobacco Fields

  • Chung, Yun-Hwa (Agro-Tech Research Group, KT&G Central Research Institute) ;
  • Yu, Yun-Hyun (Agro-Tech Research Group, KT&G Central Research Institute) ;
  • Kang, Yue-Gyu (Agro-Tech Research Group, KT&G Central Research Institute)
  • Published : 2004.12.31
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Abstract

The population of Ralstonia solanacearum (Rs) in soil is very important as a primary inoculum source of bacterial wilt in tobacco fields. To investigate the population of Rs, physical properties and chemical components during the tobacco growing season, soil samples were taken from the fifteen fields which were located in the flue-cured tobacco growing area, Ansung, Kyunggi province and Wonju, Kangwon province. Two fields of the fifteen were bacterial wilt free. Six fields had less than 10% plants being diseased and seven over 10%. The Rs population level determined by using SMSA medium generally showed an up-and-down pattern being low in May, high in Jun and July and low in August. The soil population in May and June showed a positive correlation with the incidence of bacterial wilt (r=0.571$^*$, r=0.688$^{**}$), but P$_2O_5$, content of soil was negatively correlated with the disease incidence (r=-0.539$^*$). These results suggest that Rs population in soil examined in May or in June, and the P$_2O_5$ content in soil should be key factors to determine the bacterial wilt potential of tobacco fields.

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References

  1. Chen, W-Y. and Echandi, E. 1982. Bacteriocin production, and selective medium for detection and isolation, and quantification of Pseudomonas solanacearum in soil. Phytopathology 72:310-313
  2. DeBoer, S. H. 1982. Survival of phytopathogenic bacteria in soil. In: Phytopathogenic prokaryotes Vol. 1. ed. by M. S. Mount, and G H. Lacy. pp. 285-306. Academic Press. NY
  3. Englebrecht, M. C. 1994. Modification of a semi-selective medium for isolation and quantification of Pseudomonas solanacearum. In: Bacterial wilt newsletter 10:3-5. ed. by A. C. Hayward. Austrian Centre for International Agricultural Research. Canberra AU
  4. Elphinstone, J. G, Stanford, H. M. and Stead, D. E. 1997. Detection of Ralstonia solanacearum in potato tubers, Solanum dulcamara and associated irrigation water. In: Bacterial wilt disease: molecular and ecological aspects. ed. by P. H. Prior, e. Allen, and J. G Elphinstone. pp. 133-139. Springer-Verlag, Berlin, Gennany
  5. Fortnum, B. A. and Martin, S. B. 1998. Disease management strategies for control of bacterial wilt of tobacco in the southeastern USA. In: Bacterial wilt disease: molecular and ecological aspects. ed. by P. H. Prior, C. Allen and J. a Elphinstone. pp. 394-402. Springer, Berlin, Gennany
  6. French, E. B., Gutarra, L. Aley, P. and Elphinstone, J. 1995. Culture media for Ralstonia solanacearum isolation, identification and maintenance. Fitopatologia 30:126-130
  7. Grey, B. E. and Steck, T. R. 2001. The viable but nonculturable state of Ralstonia solanacearum may be involved in long-tenn survival and plant infection. Appl. Environ. Microbiol. 67: 3866-3872 https://doi.org/10.1128/AEM.67.9.3866-3872.2001
  8. Granada, G A. and Sequeira, L. 1983a. A new selective medium for Pseudomonas solanacearum. Plant Dis. 67: 1084-I088 https://doi.org/10.1094/PD-67-1084
  9. Granada, G A. and Sequeira, L. 1983b. Survival of Pseudomonas solanacearum in soil, rhizosphere, and plant roots. Can. J. Microbiol. 29:433-440 https://doi.org/10.1139/m83-070
  10. Hayward, A. C. 1991. Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum. Annu. Rev. Phytopathol. 29:65-87 https://doi.org/10.1146/annurev.py.29.090191.000433
  11. Ito, S., Fujii, T., Usijima, Y., Tanaka, S. and Kameya-Iwaki, M. 1998. Detection of viable cells of in soil using semi-selective medium and a PCR technique. J. Phytopathol. 146:379-384 https://doi.org/10.1111/j.1439-0434.1998.tb04769.x
  12. Kang, Y.-G, Kim, K.-H. and Oh, M.-H. 2002. Development of tobacco pest control measures. 200 I Annual Tobacco Research Report (Agriculture part). Korea Ginseng & Tobacco Research Institute. pp. 1-30
  13. Lucas, G. B. 1975. Granville wilt. pp. 365-382. In: Diseases of tobacco 3rd ed. Biological Consulting Association, Raleigh NC.621pp
  14. Melton, T. A. Broadwell, A. and Wilson, J. 2004. Disease management. In: Flue-cured tobacco infonnation. pp. 142- I66. North Carolina Cooperative Extension Service. NC
  15. Shew, H. D. and Lucas, G. B. 1991. Compendium of tobacco diseases. APS Press. Mn, USA. 68pp
  16. Schroth, M. N., Thomson, S. V. and Weinhold, A. R. 1979. Behavior of plant pathogenic bacteria in rhizosphere soils. In: Ecology ofroot pathogens. ed. by S. V. Krupa, and Y. R. Dommergues. pp. 105-156. Elsiver Scientific Publishing Co. NY
  17. van Elsas, J. D. Kastelin, R, van Bekkum, R, van der Wolf, J. M.,de Vries, P. M. and van Overbeek, L. S. 2000. Survival of Ralstonia solanacearum biovar 2, the causative agent of potatobrown rot, in field and microcosm soil in temperate climates.Phytopatholosy 90:1358-1366 https://doi.org/10.1094/PHYTO.2000.90.12.1358
  18. van Overbeek, L. S., Bergervoet, H. W., Jacobs, F. H. H. and van Elas, J. D. 2004. The low-temperature-induced viable-butnonculturable state affects the virulence of Ralstonia solanacearum biovar 2. Phytopathology 94:463-469 https://doi.org/10.1094/PHYTO.2004.94.5.463

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