• 한국연초학회지
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• 제4권1호
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• pp.29-35
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• 1982

Potassium deficiency symptoms were studied with flue-cured tobacco which was applied with different levels of compound fertilizer (10-15-20) ; 75kg, 100hg, and 125kg/10a. Ratio of N/$K_2O$ in leaves was increased from bottom to top stalk position due to the increase of nitrogen content in leaves. Nitrogen content in leaves was increased from stalk to tip as wall as from midrib to laminae, but vice versa in potassium content. Consequently, resulting in potassium deficiency symptoms in tip of leaves. Rate of reabsorption by rainfall during the latter part of growth was highest at top stalk position in case of nitrogen, but lowest in potassium. This observation was more evident with higher application rate of fertilizer. Nitrogen content of about 4 % in leaves of top stalk position applied with 125kg/10a was maintained up to 85days after trans planting. No increase in potassium in upper leaves was observed over the level of 100kg/10a fertilizer application. As the result, N/$K_2O$ ratio in leaves of top stalk position applied with 125kg/10a was kept at more than 1.0 up to 85days after transplanting, but it was less than 0.9 at 65days after tracts planting with less than 100kg/10a fertilizer application.

• 한국연초학회지
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• 제20권2호
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• pp.139-143
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• 1998

The occurrence and physico-chemical properties of flat leaves found in 1997 curing trial related to the prevention of occurrence of flat leaves at bulk curing of flue-cured tobacco were investigated and compared with those of normal leaves. Cured leaves of NC82 were separated into 4 classes of none, slight, fair and severe symptoms of flat leaf by the percentage of flattened parts to whole leaf area. The flat leaves were mostly found among the leaves of lower stalk position, and it was estimated that growth rate of lower leaves also influenced on the occurrence of flat leaves. In chromatic aberration of cured leaf, flat leaves showed remarkably lower b and L values than in those of normals but there was no difference in a value. On the other hand, in chemical analyses of flat leaf samples, nicotine, total sugar, ether extract and total nitrogen contents were decreased with the degree of flat symptoms. In physical properties, filling capacity of cured leaves was decreased with the degree of flat symptoms comparing with those of normal leaves, while shatter index was showed a reverse tendency, and then within the same leaf, flat parts were decreased in total sugar, ether extract and filling capacity compared with those of normal ones, but remarkably increased in shatter index, and there was no difference in nicotine and total nitrogen contents.

• Plant Biotechnology Reports
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• 제4권1호
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• pp.75-83
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• 2010

Glycine betaine has been reported as an osmoprotectant compound conferring tolerance to salinity and osmotic stresses in plants. We previously found that the expression of betaine aldehyde dehydrogenase 1 gene (OsBADH1), encoding a key enzyme for glycine betaine biosynthesis pathway, showed close correlation with salt tolerance of rice. In this study, the expression of the OsBADH1 gene in transgenic tobacco was investigated in response to salt stress using a transgenic approach. Transgenic tobacco plants expressing the OsBADH1 gene were generated under the control of a promoter from the maize ubiquitin gene. Three homozygous lines of $T_2$ progenies with single transgene insert were chosen for gene expression analysis. RT-PCR and western blot analysis results indicated that the OsBADH1 gene was effectively expressed in transgenic tobacco leading to the accumulation of glycine betaine. Transgenic lines demonstrated normal seed germination and morphology, and normal growth rates of seedlings under salt stress conditions. These results suggest that the OsBADH1 gene could be an excellent candidate for producing plants with osmotic stress tolerance.

• 한국연초학회지
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• 제15권2호
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• pp.145-151
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• 1993

Using a flue - cured tobacco variety, KF lH9, recently bred and released to farming, effect of growth regulators(fatty alcohol and C - MH) on the change of alkaloid contents and dynamics of MH uptake and distribution were investigated. An efficient sucker control method was also pursued. Treatment of fatty alcohol at button stage remarkably increased the rate of C - MH uptake. However, except in the plants treated with 2- fold volume of C - MH, residue of C -MH was kept under maximum residue level(80 ppd). C - MH residue in stalk was higher than in foliage in general. Compared to untreated control, leaf weight per unit area was increased by the treatment even in lower leaves where vigorous transfer of C - MH from treated tips was indicated and sucker control higher than 94.9% was achieved with all treatments except for sole half volume C - MH treatment at topping stage. Alkaloid content in foliage was reduced by dual treatment of fatty alcohol and C - MH but increased in stalk and root.

• 한국연초학회지
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• 제17권1호
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• pp.20-26
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• 1995

This study was carried out to obtain the basic data which include the change of the photosynthetic rate and protein content according to growth stage in the process of senescence of tobacco plant The photosynthetic rate was the maximum with 26.31$\mu$mol.CO2/m2.sec and stomatal resistance was the minimum with 0.2552cm/sec at 15th days after leaf emergence. However, after 50 days the photosynthesis was very little occurred. During leaf developments the number of chloroplast was increased and reached at the maximum at 25th days after emergence of leaf, thereafter, it was decreased gradually. The content of protein increased continuously and showed the highest value at 15th days after leaf emergence. The degradation rate of soluble protein was more rapid than that of insoluble protein at early stage of senescence. The range of decrement in the insoluble protein was low at late stage of senescence. The content of Rubisco, the key enzyme of photoamthesis, corresponded to about 50% of soluble protein and reached to the maximum at 150 days after leaf emergence. As the senescence progressed, the content of large subunit(UV) of Rubisco showed a tendency to decrease more rapidly than that of small subunit(SSU). The total amount of amino acids was the highest at 15th days after leaf emergence.

• 한국연초학회지
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• 제2권1호
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• pp.37-52
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• 1980

신개간지 토양에 대한 인산시용 수준별 석회, 퇴비증시, 퇴비와 붕소의 혼합시용에 따른 비옥도 증진의 효과가 연초의 수량 및 품질에 미치는 영향을 2년간에 걸쳐 시험한 결과를요약하면 다음과 같다. 1. 개량제 처리의 효과는 1차년도에서 보다 2차년도에 담배 생육과 엽면적지수와 단위엽 면적중을 월등하게 증가시켰다. 3. 개량제의 시용으로 초년도의 pH, 치환성염기등이 숙전과 같은 수준으로 개선되었으며, 처리별 비옥도 증진효과는 퇴비증시구가 컸다. 3. 2차년도 시험결과에서 수량은 대조구에 비하여 15∼25% 증수되었고, 품질은 처리간에 큰 차 없으나, 개량제 시용으로 대조구에 비하여 3 - 7% 향상되었다. 수익 (10a당 수납대금)은 인산흡수계수에 대한 3 %조절량+퇴비증시구가 가장 높아 대조구보다 34%의 증가를 가져왔다.

• 한국연초학회지
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• 제15권1호
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• pp.34-48
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• 1993

This experiment were conducted to investigate heterosis and combining ability for several mutant characters by analyzing dialled crosses of flue-cured tobacco. In a dialled cross of 3 flue-cured varieties and the mutant line 83H -5, the heterosis was somewhat higher in Fl than in F2. For growth character, the heterosis was 0.28-6.03% in plant height, leaf number, leaf shape index and yield, and was 43.2% for bacterial wilt disease index. The mutant line 83H-5 showed significantly negative GCA effect for plant height, leaf width and bacterial wilt disease index in Fl and F2, leaf length in F2, and positive GCA effect for total alkaloids, total nitrogen in Fl and days to flower in F2, respectively. Specific combining ability(SCA) in 83H-5 x Hicks was significant in negative effect for leaf length(F2), number of leaves(F2), leaf shape(F1, F2), bacterial wilt(F2) and alkaloids(F1), and in 83H-5 x NC 2326 in positive effect for leaf length(F1, F2) and leaf width(F2), and for 83H-5 x NC 82 in positive effect for plant height(F1, F2) and leaf width(F2), and for 83H-5 x NC 82 in Positive effect for Plant height(F1, F2), leaf length(F2) and yield(F1, F2).

• Journal of Microbiology and Biotechnology
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• 제15권6호
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• pp.1304-1309
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• 2005

The production of therapeutic proteins for human diseases in plants results in many economic benefits, including reduced risk of animal virus contamination, high yields, and reduced production and storage costs. Human cytokines, interleukin-11 (hlL-11) and granulocyte-macrophage colony-stimulating factor (hGM-CSF), cDNAs were introduced into rice or tobacco, using either the maize ubiquitin promoter or the 35S promoter. The primary hIL-11 transgenic rice plants exhibited stunted growth and a sterile phenotype, whereas the hIL-11 transgenic tobacco plants did not. This suggests that hIL-11 expression in rice disrupts the normal growth and development of the plant. The regeneration efficiency of rice calli transformed with hGM-CSF was found to be approximately a quarter of that seen with the hIL-11, suggesting that hGM-CSF expression is more deleterious to the regeneration of rice calli than is hIL-11. However, the surviving hGM-CSF transgenic rice plants exhibited a normal phenotype of growth. Therefore, it appears that only those transgenic rice lines that expressed the human cytokines in small quantities were able to survive the selection process.

• 한국토양비료학회지
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• 제20권1호
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• pp.35-41
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• 1987

질산태질소(窒酸態窒素)를 선택흡수(選擇吸收)하는 담배식물에 대하여 흡수된 $NO_3-N$의 각조직별(各組織別) 이동양상(移動樣相)과 질산환원효소(窒酸還元酵素)의 활성도(活性度)(nitrate reductase activity. NRA)를 조사하고 생육과정(生育過程)에서 출엽(出葉)된 잎들의 질산환원능력(窒酸還元能力)을 비교조사(比較調査)한 결과(結果) 1. 도관조직(導管組織)인 엽맥(葉脈)까지는 함유(含有)된 질소(窒素)의 대부분(大部分)이 $NO_3-N$으로써 무기태질소(無機態窒素)로 엽맥(葉脈)을 통과(通過)하며 엽육(葉肉)에서는 $NO_3-N$이 환원(還元)되어 무기태질소(無機態窒素)가 거의 검출(檢出)되지 않았다. 2. NRA는 성숙(成熟)된 잎에서 가장 왕성하였으며 분열생장중(分裂生長中)인 잎이나 도관조직(導管組織)에서는 환원기능이 매우 낮은 수준에 머물렀다. 3. 하위(下位)의 성숙엽(成熟葉)은 두껍고 수분(水分)을 많이 함유(含有)하였으며 가장 왕성한 질산환원능력(窒酸還元能力)을 보였다. 4. 생장조직(生長組織)으로 유기질소물질(有機窒素物質)을 공급(供給)하기 위하여 유식물(幼植物)때는 소수(小數)의 좁은 잎에서 높은 수준(水準)의 NRA를 유지(維持)했으나 생육(生育)이 진전되어 많은 잎이 착엽(着葉)되면서 상위(上位)쪽의 성숙엽(成熟葉)은 NRA가 낮은 수준에 머물렀다.

• 한국응용곤충학회지
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• 제23권3호
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• pp.142-146
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• 1984

인삼 근부병 억제토양 및 유발토양의 추출액 배지에서 병원균인 Fusarium solani, Rhizoctonia solani, Phytophthora cactorum, Sclerotinia sp.의 균계생장과 여기에 영향을 미지는 두 토양의 근권환경을 비교하였다. 4병원균 모두 추출액을 열처리하지 않았을 때 유발토양보다 억제토양 추출액 배지에서 생장이 더 억제되였고, $100^{\circ}C$로 처리 하였을 때는 F. solani와 Sclerotinia sp.는 두 토양간에 차이가 없었으나 R. solani와 P. cactorum은 유발토양에서 균사생장이 더 좋았다. 또 억제토양 추출액의 열처리 온도를 높힐수록 모든 병원균의 생장이 증가되었다. 두 토양의 근권미생물 밀도는 Fusariumtn는 유의차가 없었으나 전세균, 진균은 모두 억제토양에 밀도가 더 높았으며 이 미생물들에 대한 Fusarium밀도의 비율도 억제토양이 더 높았다. 점토함량은 액제토양이, 모래함량은 유발토양이 각각 더 높았고, 화학성분중 Mg, Na 함량은 유발 토양이 더 많았으며 Ca, Fe, $P_2O_5$도 유의성은 없었으나 억제토양 보다 높은 경향이었다.