Plant Biotechnology Reports

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Expression of Indica rice OsBADH1 gene under salinity stress in transgenic tobacco

  • Hasthanasombut, Supaporn (Laboratory of Plant Cell Technology, Graduate School of Horticulture, Chiba University) ;
  • Ntui, Valentine (Laboratory of Plant Cell Technology, Graduate School of Horticulture, Chiba University) ;
  • Supaibulwatana, Kanyaratt (Department of Biotechnology, Faculty of Science, Mahidol University) ;
  • Mii, Masahiro (Laboratory of Plant Cell Technology, Graduate School of Horticulture, Chiba University) ;
  • Nakamura, Ikuo (Laboratory of Plant Cell Technology, Graduate School of Horticulture, Chiba University)
  • Received : 2009.10.01
  • Accepted : 2009.12.06
  • Published : 2010.03.30
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Abstract

Glycine betaine has been reported as an osmoprotectant compound conferring tolerance to salinity and osmotic stresses in plants. We previously found that the expression of betaine aldehyde dehydrogenase 1 gene (OsBADH1), encoding a key enzyme for glycine betaine biosynthesis pathway, showed close correlation with salt tolerance of rice. In this study, the expression of the OsBADH1 gene in transgenic tobacco was investigated in response to salt stress using a transgenic approach. Transgenic tobacco plants expressing the OsBADH1 gene were generated under the control of a promoter from the maize ubiquitin gene. Three homozygous lines of $T_2$ progenies with single transgene insert were chosen for gene expression analysis. RT-PCR and western blot analysis results indicated that the OsBADH1 gene was effectively expressed in transgenic tobacco leading to the accumulation of glycine betaine. Transgenic lines demonstrated normal seed germination and morphology, and normal growth rates of seedlings under salt stress conditions. These results suggest that the OsBADH1 gene could be an excellent candidate for producing plants with osmotic stress tolerance.

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References

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