• 디지털융복합연구
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• 제14권1호
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• pp.339-351
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• 2016

성적 공상은 개인의 무의식적인 측면을 반영할 뿐만 아니라 장애를 유발하거나 치료에 활용되는 등의 양면성을 갖기도 한다. 본 연구는 성적 공상의 양면성에 대해 다음과 같이 고찰하는 것을 목적으로 하였다. 첫째, 성적 공상의 정상과 비정상적인 측면이 선행연구에서 정의된 방식과 그 한계점을 살펴보았다. 둘째로, 성적 공상에 대한 강박적인 불편감, 성범죄와 성적 공상 등의 관계 등 위험 요인으로서의 문제점들을 확인하였다. 셋째, 성적 공상을 치료적인 방식으로 사용할 때의 지침과 원리 및 그 긍정적인 효과에 대해 고찰하였다. 넷째, 성적 공상의 적절한 활용을 위한 성적 공상의 측정도구와 측정방법에 대해 살펴보고 발전방향을 제시하였다. 본 연구는 성적 공상에 대한 양면적인 연구들을 정리하여 향후 연구의 방향을 제시한 것에 의의가 있으며 이와 관련한 논의를 제시하였다.

• 한국임상수의학회지
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• 제23권1호
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• pp.22-30
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• 2006

말의 근골격계 질환의 진단과 치유 경과 평가를 위한 체열촬영술의 유용성을 검토코자 실험적으로 유발한 감염성 관절염의 치료 과정을 조사하였다. 실험적으로 완관절에 감염성 관절염을 유발하여 항생제와 봉독으로 처치한 후, 치료 과정별로 임상검사, 실험실 검사, 방사선 검사와 병행하여 실시한 체열촬영술은 전반적으로 모든 진단 결과와 유사하게 나타났으며, 진단 시 조직에 대한 비접촉성, 비침습성 및 무통성 특성과 함께 치유경과를 시각화, 객관화하므로 효과적인 것으로 판단되었다. 따라서 말의 근골격계 질환의 체열학적 평가는 기존의 검사나 진단 영상 장치와 더불어 말의 근골격계 질환의 진단 및 치유경과 평가에 있어서 유용할 것으로 사료된다.

• The Korean Journal of Physiology and Pharmacology
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• 제13권5호
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• pp.373-378
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• 2009

Cerebellar Purkinje cells (PCs) play a crucial role in motor functions and their progressive degeneration is closely associated with spinocerebellar ataxias. Although immunohistochemical (IHC) analysis can provide a valuable tool for understanding the pathophysiology of PC disorders, the method validation of IHC analysis with cerebellar tissue specimens is unclear. Here we present an optimized and validated IHC method using antibodies to calbindin D28k, a specific PC marker in the cerebellum. To achieve the desired sensitivity, specificity, and reproducibility, we modified IHC analysis procedures for cerebellar tissues. We found that the sensitivity of staining varies depending on the commercial source of primary antibody. In addition, we showed that a biotin-free signal amplification method using a horseradish peroxidase polymer-conjugated secondary antibody increases both the sensitivity and specificity of ICH analysis. Furthermore, we demonstrated that dye filtration using a $0.22\;{\mu}m$ filter eliminates or minimizes nonspecific staining while preserving the analytical sensitivity. These results suggest that our protocol can be adapted for future investigations aiming to understand the pathophysiology of cerebellar PC disorders and to evaluate the efficacy of therapeutic strategies for treating' these diseases.

• Journal of Microbiology and Biotechnology
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• 제20권10호
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• pp.1463-1470
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• 2010

E. coli has long been widely used as a host system for the manufacture of recombinant proteins intended for human therapeutic use. When considering the impurities to be eliminated during the downstream process, residual host cell DNA is a major safety concern. The presence of residual E. coli host cell DNA in the final products is typically determined using a conventional slot blot hybridization assay or total DNA Threshold assay. However, both the former and latter methods are time consuming, expensive, and relatively insensitive. This study thus attempted to develop a more sensitive real-time PCR assay for the specific detection of residual E. coli DNA. This novel method was then compared with the slot blot hybridization assay and total DNA Threshold assay in order to determine its effectiveness and overall capabilities. The novel approach involved the selection of a specific primer pair for amplification of the E. coli 16S rRNA gene in an effort to improve sensitivity, whereas the E. coli host cell DNA quantification took place through the use of SYBR Green I. The detection limit of the real-time PCR assay, under these optimized conditions, was calculated to be 0.042 pg genomic DNA, which was much higher than those of both the slot blot hybridization assay and total DNA Threshold assay, where the detection limits were 2.42 and 3.73 pg genomic DNA, respectively. Hence, the real-time PCR assay can be said to be more reproducible, more accurate, and more precise than either the slot blot hybridization assay or total DNA Threshold assay. The real-time PCR assay may thus be a promising new tool for the quantitative detection and clearance validation of residual E. coli host cell DNA during the manufacturingprocess for recombinant therapeutics.

• 가정∙방문간호학회지
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• 제13권1호
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• pp.5-15
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• 2006

Purpose : This study was done to examine the home care service provided by home care nurses and the level of client satisfaction in community. Method : Data were collected from 120 clients who received the home care service at the community home care service center in Pusan from 2th May. 2003 to 31th May 2003. The tool for measurement of satisfaction was composed of 16 items and was 4 score scale by Kim. Data were analyzed by using SPSS/WIN 10.5 program. Result: 1) 67.5% among 120 clients was over sixty years old and 30.6% of clients received home visits 20 times by home care nurse. 2) The total number of home care services was 15,783. And most of the clients received therapeutic nursing care. 3) The mean score of satisfaction on provided home care services was 3.31, among 16 items, "The home care nurses kept up the secret of patients and family" was the highest($3.67{\pm}0.48$). the total level of satisfaction of home care services was very high, with mean score $3.31{\pm}0.32$. Conclusion : The satisfaction level of home care services was very high. so we can expect that the prospect of home care services is very challengeable. Therefore we should try to expand the service recipients with promotional education to assure close ties with the public health centers for the continuous home care service linking and make more efforts to improve the quality of the service.

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book II
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• pp.508-518
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• 2003

SZ95 cell is an immortalized human sebaceous gland cell line that shows the morphologic, phenotypic and functional characteristics of normal human sebocytes. Sebocytes may play crucial parts in the pathophysiologic processes and disorders of the pilosebaceous unit. The secretory activity of the sebaceous gland is remarkably species-specific and acne is an exclusively human disease. Thus, this SZ95 cells offer possibilities for investigations on the physiology of the sebaceous gland and its role in sebum-associated skin disease such as acne. In this study, we investigated the effects of 13-cis-retinoic acid (13-cis-RA) and spironolactone, frequently used as therapeutic agents of acne, on the lipid synthesis and proliferation of human sebocytes. Cell proliferation was determined by MTT assay and cytoplasmic lipid droplets was shown by Oil-red a staining. Total lipid levels were biochemically estimated by the sulfo-phospho-vanilline reagent. 13-cis-RA and spironolactone significantly inhibited proliferation and lipid levels in a dose-dependent manner. Combined treatment with testosterone and 13-cis-RA or spironolactone resulted in a lower total lipid levels than that with androgen alone. These observations indicate that 13-cis-RA and spironolactone are potent inhibitors of both cell proliferation and lipid synthesis in human sebocytes. We will provide experimental evidence that this human sebocyte cell line serves as an adequate tool for evaluating the anti-lipogenic activity of various compounds potentially useful for the bioactive cosmeceutical ingredients on acne skin, and studying the intracellular biochemical markers depending on the types of compounds from various sources.

• The Korean Journal of Pain
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• 제18권1호
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• pp.43-47
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• 2005

Background: The optimal management of pain using magnetic irradiation modalities continues to attract considerable debate. Therefore, we conducted a randomized, double-blind, placebo-controlled clinical trial to determine the effectiveness of pulsed electromagnetic therapy for the treatment of chronic lower back pain. Methods: Fifteen-minute sessions of active (n = 20) or placebo (n = 20) electromagnetic therapy were repeated 3 times a week for 3 weeks. Patients were assessed using the 11-point numerical rating scale and the revised Oswestry disability scores for up to 4 weeks after therapy. Results: The active magnetic group showed significantly more pain reduction than the placebo group immediately after therapy and one and four weeks after therapy (P < 0.05). At 4 weeks after therapy percentage changes in NRS from baseline were $22{\pm}24%$ and $38{\pm}11%$ in the placebo and magnetic groups, respectively. The revised Oswestry disability percentage in the active magnetic group was also significantly improved (P < 0.05). Conclusions: Pulsed electromagnetic therapy provided pain relief and ameliorated disability in patients with chronic lower back pain. According to our results, pulsed electromagnetic therapy should be considered an important potential therapeutic tool for the conservative therapy of chronic lower back pain.

• The Korean Journal of Physiology and Pharmacology
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• 제19권4호
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• pp.349-355
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• 2015

We examined the effects of peripherally or centrally administered botulinum neurotoxin type A (BoNT-A) on orofacial inflammatory pain to evaluate the antinociceptive effect of BoNT-A and its underlying mechanisms. The experiments were carried out on male Sprague-Dawley rats. Subcutaneous (3 U/kg) or intracisternal (0.3 or 1 U/kg) administration of BoNT-A significantly inhibited the formalin-induced nociceptive response in the second phase. Both subcutaneous (1 or 3 U/kg) and intracisternal (0.3 or 1 U/kg) injection of BoNT-A increased the latency of head withdrawal response in the complete Freund's adjuvant (CFA)-treated rats. Intracisternal administration of N-methyl-D-aspartate (NMDA) evoked nociceptive behavior via the activation of trigeminal neurons, which was attenuated by the subcutaneous or intracisternal injection of BoNT-A. Intracisternal injection of NMDA up-regulated c-Fos expression in the trigeminal neurons of the medullary dorsal horn. Subcutaneous (3 U/kg) or intracisternal (1 U/kg) administration of BoNT-A significantly reduced the number of c-Fos immunoreactive neurons in the NMDA-treated rats. These results suggest that the central antinociceptive effects the peripherally or centrally administered BoNT-A are mediated by transcytosed BoNT-A or direct inhibition of trigeminal neurons. Our data suggest that central targets of BoNT-A might provide a new therapeutic tool for the treatment of orofacial chronic pain conditions.

• 한국동물위생학회지
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• 제23권2호
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• pp.125-132
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• 2000

To establish the differential diagnosis and treatment method in bovine ovarian cysts, specially ovarian cysts with corpus luteum, serum progesterone ($P_4$) concentration and ultrasonography for measuring the cystic wall thickness and diameter of cyst and corpus luteum were investigated from slaughtered cows with ovarian cysts. Ovarian cysts were classified 8 types by the number of cyst, cystic wall thickness and present of corpus luteum. Ovarian cysts with corpus luteum were 11 (13.6%) of 81 cows and ovarian cysts without corpus luteum were 70 (86.4%) cows. The incidence rates of 8 various types of ovarian cysts were as follows; 2Ba 33.3%, 2Aa 25.9% and 2Bb 14.8%, respectively The Incidence rates of ovarian cysts without corpus luteum were follicular cyst 59.2% and luteal cyst 27.2%. The cystic wall thickness were 2Ab 3.7mm and 2Bb 3.5mm, and the serum P4 concentrations were above 2.0 ng/$m\ell$ in IAa, tAb, IBa, 2Ab and 2Bb, respectively In ovarian cysts with corpus luteum, the correlation coefficients between corpus luteum area and serum $P_4$ concentration were 0.45. In ovarian cysts without corpus luteum, there was significantly positive correlations between cystic wall thickness and serum $P_4$ concentration($r^2$ = 0.54, p<0.01). These results indicate that $PGF_2$$\alpha$ analogues can be choice for treatment of ovarian cysts with corpus luteum and above 3mm the cystic wall thickness because serum $P_4$ concentrations were above 2.0 ng/$m\ell$ in ovarian cysts with corpus luteum and thickened cystic wall. In conclusion, it Is suggested that ultrasonography is useful diagnostic tool for diagnosis and selection of treatment remedy in cystic ovaries of bovine.

• 대한불안의학회지
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• 제14권2호
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• pp.63-70
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• 2018

It has been suggested that aberrant self-referential processing (SRP) is one of the important components of the explanatory models of social anxiety disorder (SAD). The default mode network (DMN), which reflects intrinsic brain functions, is known to play a critical role in SRP. Recently, resting state functional magnetic resonance imaging (fMRI) research on the functional connectivity in the brain network has gained greater attention as a tool to elucidate the neurobiological basis of various psychiatric disorders. We reviewed resting state fMRI studies that investigated the resting state functional connectivity (RSFC) of the DMN in SAD. Despite of the heterogeneity of the analytic methods and occasional negative findings, most studies consistently reported abnormalities of RSFC within the DMN, suggesting that the DMN may be significant neural correlates of aberrant SRP in SAD. Also, changes in RSFC of the DMN are associated with clinical improvements of therapeutic interventions. Moreover, emerging findings provide the basis for potential use of RSFC as a complementary method in diagnosis of SAD. Ongoing and future research to investigate RSFC of the DMN could broaden our understanding regarding the neurobiological basis of SAD, and contribute to the development of novel treatments for SAD.