• Title/Summary/Keyword: theoretical yield

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Construction of a Thermotolerant Saccharomyces cerevisiae Strain for Bioethanol Production with Reduced Fermentation Time and Saccharifying Enzyme Dose

  • Lim, Ji Sung;Jang, You Ri;Lim, Young Hoon;Kim, Keun
    • Journal of Microbiology and Biotechnology
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    • v.22 no.10
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    • pp.1401-1405
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    • 2012
  • A thermotolerant Saccharomyces cerevisiae mutant strain, TT6, was constructed after multi-parental hybridization of five mutant strains obtained by UV or NTG treatment of the original strain, S. cerevisiae KV1. When incubated at $40^{\circ}C$ in YPD broth, TT6 began to grow exponentially in 10 h, but KV1 did not show any noticeable growth even after 22 h. The thermotolerant growth of TT6 was confirmed by serial dilution assay at $42^{\circ}C$; TT6 grew at a cell concentration ($10^{-5}$) 10,000 times lower than that of KV1 ($10^{-1}$). Whereas ethanol production from YP containing 23% (w/v) glucose by KV1 decreased with increasing temperature from $30^{\circ}C$ to $36^{\circ}C$, ethanol production by TT6 did not decrease at temperatures up to $37^{\circ}C$. When TT6 was tested for ethanol production at $36^{\circ}C$ by simultaneous saccharification and fermentation (SSF) from 23% corn, 24 h of fermentation time or 50% of the glucoamylase dose was saved when compared with KV1 at $30^{\circ}C$. The ethanol yield from corn by SSF with TT6 at $36^{\circ}C$ was 91.7% of the theoretical yield, whereas that of KV1 at $30^{\circ}C$ was 90.6%.

Population Dynamics of Corbicula ( Corbicula) japonica Prime from Namdae Stream in Yangyang, Korea (강원도 양양 남대천에서 채집된 일본재첩, Corbicula (Corbicula) japonica Prime의 수산자원학적 연구)

  • Kwon Dae Hyeon;Kang Yong Joo;Kim Wan Ki;Lee Chae Sung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.35 no.6
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    • pp.686-695
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    • 2002
  • Samples of Corbicula ( Corbicula) japonica Prime of Namdae Stream in Gangnung were collected from November 2000 to October 2001. Age of C. (C.) japonica was determined from the rings on the shell, The shell length of the samples ranged from 8 mm to 38 mm. The ring on the shell was formed once a year in March. Von Bertalanffy's growth parameters were estimated using a nonlinear regression method, asyinptotie shell length ($L_{\omega}$) was 48,98 mm, K was 0.20421year, theoretical age at 0 shell length $(t_0)$ was 0.3169 year, and asymptotic total weight ($W_{\omega}$) was 41.37 g. The formula of allomeky between shell length (L, mm) and total weight (W, g) of the brackish water clam was W=3.42$\times$10^{-4}L^{3}. The annual survival rate was estimated at 0.3799, instantaneous coefficient of natural mortality was 0.5007/year, and instantaneous coefficient of fishing mortality was 0.46721year. The age at first capture was estimated at 2.1593 year using shell length compositions of the brackish water clam, The current yield-per-recruit at 0.4672/year of fishing mortality was 0.6595 g. F_0.1 was estimated at 0.1865/year, Acceptable biological catch was estimated at 14.4 metric ton.

A Study on Development of Structural Health Monitoring System for Steel Beams Using Strain Gauges (변형률계를 이용한 강재보의 건전도 평가 시스템 개발에 관한 연구)

  • Hahn, Hyun Gyu;Ahn, Hyung Joon
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.16 no.1
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    • pp.99-109
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    • 2012
  • This study aimed to develop a Structural Health Monitoring System for steel beams in the manner of suggesting and verifying a theoretical formula for displacement estimation using strain gauges, and estimating the loading points and magnitude. According to the results of this study, it was found that when a load of 160kN (56% of the yield load) was applied, the error rate of the deflection obtained with a strain gauge at the point of maximum deflection compared to the deflection measured with a displacement meter was within 2%, and that the estimates of the magnitude and points of load application also showed the error rate of not more than 1%. This suggests that the displacement and load of steel beams can be measured with strain gauges and further, it will enable more cost-effective sensor designing without displacement meter or load cell. The Structural Health Monitoring System program implemented in Lab VIEW gave graded warnings whenever the measured data exceeds the specified range (strength limit state, serviceability limit state, yield strain), and both the serviceability limit state and strength limit state could be simultaneously monitored with strain gauge alone.

Enantioselective Hydrolysis for Preparing (S)-Styrene Oxide in Organic Solvents Using Recombinant Escherichia coli Expressing Protein-engineered Epoxide Hydrolase of Mugil cephalus (Mugil cephalus 유래 에폭사이드 가수분해효소를 발현하는 재조합 대장균을 이용한 유기용매에서의 (S)-Styrene Oxide 제조를 위한 입체선택적 가수분해 반응)

  • Lee, Ok Kyung;Lee, Eun Yeol
    • Applied Chemistry for Engineering
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    • v.23 no.6
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    • pp.599-603
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    • 2012
  • The enantioselective hydrolysis of racemic styrene oxide in organic solvents was conducted using a recombinant E. coli expressing protein-engineered Mugil cephalus epoxide hydrolase (McEH). The volumetric total activity of the recombinant E. coli was enhanced 2.2-fold by IPTG induction at a mid-exponential growth phase. Among organic solvents with different log P values, isooctane was chosen based on the high activity and the enantioselectivity of McEH. Some lyoprotectants such as skim milk or sucrose enhanced the McEH activity. Enantiopure (S)-Styrene oxide with a 98% ee was obtained from the racemic styrene oxide with a 53.6% yield based on its theoretical yield in isooctane.

Recent Progress in Strain Development of Zymomonas mobilis for Lignocellulosic Ethanol Production (Zymomonas mobilis를 이용한 목질계 에탄올 생산을 위한 균주 개선에 관한 연구 동향)

  • Jeon, Young Jae
    • Journal of Life Science
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    • v.29 no.1
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    • pp.135-145
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    • 2019
  • Zymomonas mobilis has been recognized as a potential industrial ethanologen for many decades due to its outstanding fermentation characteristics, including high ethanol tolerance, fast sugar uptake rate, and high theoretical ethanol yield. With the emergence of the postgenomic era and the recent announcement of DuPont's world largest cellulosic ethanol production process, research on this bacterium has become even more important to harness successful application not only for use in the bioethanol process but also in other biochemical processes, which can be included in bio-refinery. As an important industrial microorganism, Z. mobilis will likely be exposed to various stressful environments, such as toxic chemicals, including the end-product ethanol and fermentative inhibitory compounds (e.g., furan derivatives, organic acids, and lignin derivatives in pretreatment steps), as well as physical stresses, such as high temperature during large-scale ethanol fermentation. This review focuses on recent information related to the industrial robustness of this bacterium and strain development to improve the ethanol yield and productivity in the lignocellulosic ethanol process. Although several excellent review articles on the strain development of this bacterium have been published, this review aims to fill gaps in the literature by highlighting recent advances in physiological understanding of this bacterium that may aid strain developments and improve the ethanol productivity for lignocellulosic biomass.

J2-bounding Surface Plasticity Model with Zero Elastic Region (탄성영역이 없는 J2-경계면 소성모델)

  • Shin, Hosung;Oh, Seboong;Kim, Jae-min
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.43 no.4
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    • pp.469-476
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    • 2023
  • Soil plasticity models for cyclic and dynamic loads are essential in non-linear numerical analysis of geotechnical structures. While a single yield surface model shows a linear behavior for cyclic loads, J2-bounding surface plasticity model with zero elastic region can effectively simulate a nonlinearity of the ground response with the same material properties. The radius of the yield surface inside the boundary surface converged to 0 to make the elastic region disappear, and plastic hardening modulus and dilatancy define plastic strain increment. This paper presents the stress-strain incremental equation of the developed model, and derives plastic hardening modulus for the hyperbolic model. The comparative analyses of the triaxial compression test and the shallow foundation under the cyclic load can show stable numerical convergence, consistency with the theoretical solution, and hysteresis behavior. In addition, plastic hardening modulus for the modified hyperbolic function is presented, and a methodology to estimate model variables conforming 1D equivalent linear model is proposed for numerical modeling of the multi-dimensional behavior of the ground.

Biological Yielding Potential of Rice in Association with Climatic Factors in Yeongnam Region (영남지역 기상과 수도의 한계생산력 해석)

  • Kim, Soon-Chul;Lee, Soo-Kwan;Chung, Geun-Sik
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.30 no.3
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    • pp.259-270
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    • 1985
  • Meteorological year variations for rice crop from 1973 to 1984 were compared by using air temperature and sunshine hour for nursery period, cooling index for reproductive stage and meteorological yield productivity index for ripening period. The most optimum transplanting date and heading date for crop yield based on real transplanting date-grain yield relationship or heading date-grain yield relationship, meteorological yield productivity index and actual results showed good agreement each other. Around May 26 for transplanting and August 10 for heading were the most optimum date in Indica/Japonica hybrid cultivars while these were about June 8 and August 23 for Japonica cultivars, respectively. On the other hand, theoretical late limiting heading date for safe ripening were August 20 for Indica/Japonica hybrid cultivars and August 30 for Japonica cultivars, respectively, for both methods, cumulative temperature method during ripening with 80% believable frequency and meteorological yield productive index method having 1000(kg/10a) yielding potential. Based on the yield forecast trial, the highest values of photosynthetic efficiency, 2.5%, and crop growth rate, 23g/㎡/day, were recorded during 30 days before rice heading. Considering the photosynthetic efficiency and solar radiation, the potential crop growth rate was more or less 30g/㎡/day and the biological grain yielding potential in a existing cultural practices was approximately 900-1000(kg/10a) in Milyang weather condition. To increase further yielding potential, either photosynthetic efficiency or harvest index or both should be improved by manipulating appropriate canopy architecture, plant spacing, fertilizer, chemical, etc.

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Determinants of Department Store Sales Commissions Under Consignment Contracts: An Integrated Perspective (백화점 특약매입 거래에서 판매수수료의 결정요인 : 거래비용, 힘-의존이론과 자원기반이론의 통합적 관점)

  • Yi, Ho-Taek;Yeom, Min-Sun;Seo, Hun-Joo
    • Journal of Distribution Science
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    • v.13 no.11
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    • pp.47-58
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    • 2015
  • Purpose - This study aims to seek determinants of department store sales commission rates under consignment contracts based on transaction cost theory, the power-dependence view, and the resource-based view. A consignment contract is a unique contract where the retailer, over a given period, takes possession of goods owned by a supplier, promotes the sales of these goods, and receives a profit share from their sales. Under this contract, the supplier owns the goods until they are sold. In department stores in South Korea, over 70% of overall sales comes through consignment contracts. In other words, this is the most popular contract agreement between large retailers and vendors in South Korea. Consignment contracts yield high profits to department stores with minimal sales uncertainty, stock cost, and marketing investment. Many suppliers believe the consignment contract commission rates are too high. However, department stores disagree. They state that the commissions are not high as they generate new value for the suppliers by accumulating up-to-date merchandise and supporting various marketing programs on their behalf. Recently, consignment contracts have been critically examined and scrutinized by politicians, mass media, and the public of Korea. This study further intends to derive implications reflecting both buyer and seller perspectives as well as offer insights to policy makers in making appropriate decisions. Research design, data, and methodology - To verify the proposed research model and test hypotheses, the authors selected 164 suppliers, which currently have relationships with department stores. This study carefully investigated the reliability, content validity, convergent validity, and discriminant validity of the proposed model. The data were analyzed using SPSS 18.0 and AMOS structural equation modeling program Results - For the transaction cost theory and the power-dependence view, the results indicated that product diversity and demand volatility had a positive impact on the sales dependence on a department store. Dependence in turn had a positive effect on the sales commission under the consignment contract. Based on the resource-based view, the department store's marketing capability, the supplier's perception toward merchandising, and supporting activities could enhance the department store's channel leadership in the buyer-seller relationship. Subsequently, the channel leadership had a positive effect on the sales commission. However, product complexity had no relationship with department store dependence. Conclusions - This is the first empirical research that investigates the determinants of sales commissions under consignment contracts in the domestic retail industry. This study reveals several theoretical and practical implications for both marketing scholars and marketers. In terms of theoretical implication, this study integrated and enlarged certain theoretical background, such as transaction cost theory, the power-dependence view, and the resource-based view, to explain the determinants of sales commissions under consignment contracts that include sales revenue. From a business management viewpoint, this research offers useful insights for policy makers by applying two different perspectives, both the manufacturer and the retailer, in terms of the sales commission issue under a consignment contract.

Production of Methane from Anaerobic Fermentation of Marine Macro-algae (해조류의 혐기성 발효를 이용한 메탄 생산)

  • Kim, Jeong-Min;Lee, Yeung-Ho;Jung, Sung-Hoon;Lee, Jin-Tae;Cho, Moo-Hwan
    • Clean Technology
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    • v.16 no.1
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    • pp.51-58
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    • 2010
  • Methane was produced from the anaerobic digestion of marine macro-algae. Elemental analysis was first performed to estimate the theoretical methane production of three macro-algae (Undaria pinnatifida, Laminaria japonica, Hizikia fusiformis). Three algae were found to contain C 34 ~ 36%, H 5%, O 37 ~ 43%, N 2 ~ 4%, S 0.4 ~ 0.7%, and ash 14~21%, and the theoretical methane content was in the range of 56 ~ 60%, which can produce 442 ~ 568 mL $CH_4$ per g of volatile solid (VS). Using the biological methane potential (BMP) test, we found that L. japonica resulted in the highest yield of methane (52%). Moreover, various operational conditions, such as algae amount, pH, salinity, particle size, and pre-treatment, were investigated in order to find an optimal condition of anaerobic digestion. At pH 8.0, the autoclaved L. japonica (5g VS/200 mL), when used without washing salt, produced 268.5 mL/g VS which is 65% of the theoretical methane productions. Furthermore, using a CSTR (with the working volume of 7 L out of the total volume of 10 L), we have successfully operated the reactor for 65 days and obtained maximum methane production rate of 1.4 L/day with purity of 70%.

Two-step High Temperature Pretreatment Process for Bioethanol Production from Rape Stems (유채대의 이단 고온 처리에 의한 알콜 발효용 당화물 생산)

  • Han, Jae-Gun;Oh, Sung-Ho;Jeong, Myoung-Hoon;Kim, Seung-Seop;Seo, Hyeon-Beom;Jeong, Kyung-Hwan;Jang, Young-Seok;Kim, Il-Cheol;Lee, Hyeon-Yong
    • KSBB Journal
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    • v.24 no.5
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    • pp.489-494
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    • 2009
  • Two-step pretreatment process was investigated to efficiently hydrolyzed rape stems for obtaining fermentable sugars. The process was consisted of two consecutive steps as $200^{\circ}C$ and 15 MPa and $374^{\circ}C$ and 24 MPa with the flow rate of 5 mL/min. Under this condition, 5.5 (g/L) of glucose and 25.6 (g/L) of xylose were obtained from rape stems, showing 18% of glucose yield based on 25% cellulose in the rape stems. It was also found that this process could generate less amounts of toxic residues, such as HMF (Hydroxy- Methyl-Furfural) and other fulfural components during hydrolysis process. It could reaction maintain relatively high ethanol production yield as 90% of theoretical conversion yield from glucose. Therefore, this pretreatment process could be applied to hydrolyze other cellulosic and marine resources such as woods, stem and algae for bioethanol production.