• Parasites, Hosts and Diseases
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• 제57권1호
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• pp.61-67
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• 2019

Cystic echinococcosis (CE) in sheep is a hazardous zoonotic parasitic disease that is caused by Echinococcus granulosus (Eg). At present, serological test is an important diagnostic method for Eg infection in domestic animals. Here, a fusion protein Eg mefAg-1 harboring 8 dominant B-cell epitopes of Eg such as antigen B, tetraspanin 1, tetraspanin 6, reticulon and Eg95 was produced in E. coli and evaluated for CE in sheep by indirect ELISA. Eg mefAg-1 showed in ELISA a high sensitivity (93.41%) and specificity (99.31%), with a coincidence rate of 97.02%. Overall, it is suggested that the Eg mefAg-1 could be a potential antigen candidate for CE serodiagnosis in sheep.

• Advances in aircraft and spacecraft science
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• 제5권3호
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• pp.295-318
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• 2018

This research studies thermo-elastic behavior of rotating micro discs that are employed in various micro devices such as micro gas turbines. It is assumed that material is functionally graded with a variable profile thickness, density, shear modulus and thermal expansion in terms of radius of micro disc and as a power law function. Boundary condition is considered fixed-free with uniform thermal loading and elastic field is symmetric. Using incompressible material's constitutive equation, we extract governing differential equation of four orders; to solution this equation, we utilize general differential quadrature (GDQ) method and the results are schematically pictured. The obtained result in a particular case is compared with another work and coincidence of results is shown. We will find out that surface effect tends to split micro disc's area to compressive and tensile while nonlocal parameter tries to converge different behaviors with each other; this convergence feature make FGIMs capable to resist in high temperature and so in terms of thermo-elastic behavior we can suggest, using FGIMs in micro devices such as micro turbines (under glass transition temperature).

• 한국환경과학회지
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• 제20권9호
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• pp.1165-1182
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• 2011

The evaluation of potential submarine groundwater is an important research topic for exploring an alternative water resource. Two different approaches, water budget analysis and Rn mass balance method, were employed to investigate the annual variation of submarine groundwater discharge in 2010 at a marine watershed located at the south-eastern part of Korean Peninsula. In order to obtain reliable hydrological data during study period, temporal and spatial variations of rainfall and soil moisture had been collected and hydro-meterological data such as temperature, humidity and wind speed were collected The runoff response was simulated using SCS-CN method with spatial distributions of landuse and soil texture from GIS analysis. Six different methods were used to estimate the monthly variation of evapotranspiration and field measurements of soil moisture were used to account for the infiltration. Comparisons of infiltration and surface runoff between simulation and water balance with measurements showed coincidence. The water budget analysis and Rn mass balance method provide mean daily submarine groundwater as 5.35 and 4.07 $m^3/m/day$ in 2010, respectively.

• 한국약용작물학회지
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• 제14권2호
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• pp.117-123
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• 2006

The purpose is to study the identification method of Huangjinju powder for injection and the medicinal materials by the fingerprint off-ray Diffraction Fourier (XRDF). We used the same method on both the studying of Huangjinju and the medicinal materials. Then we selected a few components alignment to compare. We analyzed the data by setting up the deviation $d({\AA})$ as ${\pm}0.05$ to calculate the rate of special mark on the sample (Px) and on the patent (P). The special XRDF of Huangjinju$[d({\AA})/(I/I_0)]$ have 5 peaks that have not expressed in medicinal materials. Therefore Px is 22.73%. Flos Trollii Chinensis has 3 special marks and Px is 17.65%. Flos Chrysanthemi Indici has 1 special mark and Px is 3.57%. Its coincided interplanar spacing with the patent is $2.907{\AA}$. Flos Lonicerae Japonicae has 6 special marks and Px is 23.08%. Its special mark in the patent are 4.95/14 and 4.50/15, respectively. The P is 9.09%. Its coincided interplanar spacing with the patent is $2.910{\AA}\;and\;3.05{\AA}$, respectively. The number of special XRDF mark peaks of baicalin is 9 and Px is 18.37%. Its coincided interplanar spacing with the patent is $2.910{\AA}$. It has visible mark and specificity adopting XRDF fingerprint to identify Huangjinju and medicinal materials. Establishing the quality standard is a synthetic index that depends both on special marks in the medicinal materials of the patent and on the coincidence peak data.

• Nuclear Engineering and Technology
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• 제54권7호
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• pp.2592-2598
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• 2022

Research groups in the field of PET instrumentation are studying time-of-flight(TOF) technology to improve the signal-to-noise ratio of PET images. Scintillation light transport and collection plays an important role in improving the coincidence resolving time(CRT) of PET detector based on a pixelated crystal array. Four crystal arrays were designed by the different optical reflection configuration such as external reflectors and surface treatment on the CRT and compared with the light output, energy resolution and CRT. The design proposed in the study was composed of 8 × 8 LYSO crystal array consisted of 3 × 3 × 15 mm³ pixels. The entrance side was roughened while the other five surfaces were polished. Four sides of all crystal pixels were wrapped with ESR-film, and the entrance surface was covered by Teflon-tape. The design provided an excellent timing resolution of 210 ps and improved the CRT by 16% compared to the conventional method using a polishing treatment and ESR-film. This study provided a method for improving the light output and CRT of a pixelated scintillation crystal-based brain TOF PET detector. The proposed configuration might be an attractive detector design for TOF brain PET requiring fast timing performance with high cost-effectiveness.

• 한국전자파학회논문지
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• 제21권2호
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• pp.170-178
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• 2010

논문에서는 전원부에서 노이즈가 발생되어 신호선에 노이즈가 유기될 때, 유전체의 손실 특성이 노이즈에 미치는 영향에 관해서 연구를 하였다. 이를 분석하기 위해 Full-wave 시뮬레이터인 Ansoft사의 HFSS(High Frequency Structure Simulation)와 CST사의 MWS(MicroWave Studio)의 계산 결과와 측정 결과를 비교하여 신뢰성을 확보하였고, 실제 사용되고 있는 4가지의 상용 기판에 대한 유기되는 노이즈를 해석하였다. 또한, TLM(Transmission Line Method)를 이용해서 전원면의 회로 모델 구성 시 기판의 유전체 손실을 반영할 수 있는 Debye 모델을 적용하여 주파수에 대한 임피던스를 분석할 수 있는 모델을 적용 측정 결과와 3 GHz까지 일치하는 모델을 얻었다.

• 대한수의학회지
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• 제43권4호
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• pp.677-681
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• 2003

Currently, both the indirect fluorescent antibody test (IFAT) and enzyme linked immunosorbent assay (ELISA) have been used to detect Neospora caninum antibodies. Several factors such as the buffers, the conjugate, the pattern of fluorescence, and the cross reactivity with other apicornplexan protozoan, may result in poorly correlated data. The present study was undertaken to develop and evaluate the Neospora agglutination test (NAT) for the detection and quantification of IgG antibodies to N. caninum from various animal species. Compared to the ELISA method, the NAT with a cutoff value of 1:512 gave a high index of coincidence (kappa=0.807) and no cross reactivity to Toxoplasma gondii antiserum. Hence, this NAT method, which did not require a species-specific secondary antibody and expensive tools, would be easily available for the detection of antibodies to N. caninllm of various animal species.

• KSBB Journal
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• 제18권1호
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• pp.39-44
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• 2003

유전자보유 유무에 따른 계통수와 16S rRNA에 의한 계통수를 염기서열 분석이 완료된 33종의 미생물에 대하여neighbor joining method와 bootstrap method(n=1,000)를 이용하여 상관관계를 분석하였다. 각 분류그룹에서 공통적으로 보존된 COG와 각 미생물이 보유하고 있는 ortholog 수에 대한 비율을 조사한 결과, Mezorhiaobium lot의 4.60% Mycoplasma genitalium의 56.57% 사이에 분포하는 것으로 파악되었다. 이는 미생물 종류에 따라서 공통 유전자의 보유정도가 차이를 보이는 것으로 독특한 유전자를 탐색할 수 있는 가능성을 제시하는 결과로 사료되었다. 그리고 같은 종 내에 서도 20% 이상의 ortholog가 서로 독립적인 것을 알 수 있었다. Archaeabacteria와 Proteobacteria 그리고 Firmicutes모두 유전자보유 계통수와 16S rRNA 계통수가 일치하는 부분과 일치하지 않는 부분으로 나뉘어진다는 것을 알 수 있었다. 이러한 결과는 165 rDNA처림 보존적이지 않은 유전자까지 고려한 결과이거나 horizontal gene transfer에 의한 영향 등으로 사료되었다. COC에 기초한 유전자보유 계통수는 생화학 적 실험과 염기서열에 기초한 분류의 중간자적 입장에서 유용유전자 탐색에 이용될 수 있을 것이다.

• 한국식품과학회지
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• 제46권5호
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• pp.538-543
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• 2014

가공식품 중 새우의 검출을 위한 샌드위치 ELISA(sELISA)의 조건을 확립하기 위해 홍다리 새우의 tropomyosin에 대한 특이항체를 이용하여 하였다. 이때, 새우의 검출범위는 1-100 ppm (${\mu}g/mL$)이며, 검출한계는 0.3 ppm이었다. 특이항체의 교차반응 결과, 홍다리새우, 흰다리 새우, 칵테일 새우, 바다가재, 꽃게에 대한 반응성은 각각 100, 73, 155, 18, 0%를 나타내었으며 새우에 대한 특이성이 매우 높았다. 열처리한 시료와 항체간의 반응성은 $100^{\circ}C$까지는 121-221%로 안정하였으나 $121^{\circ}C$에서는 반응성이 급격히 감소하였다(7.8%). 크림스프, 이유식, 소시지, 어묵, 소스에 대한 spike test에서 새우의 분석회수율은 각각 397, 639, 168, 234, 0%로 소스를 제외하고 매우 높게 나타났다. sELISA에 의하여 14점의 시판시료 중 새우의 함유 유무를 조사한 결과, 정성적으로 원료의 표시사항과 일치하는 비율은 79%이었다.

• 한국환경생태학회지
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• 제31권2호
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• pp.202-219
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• 2017

본 연구는 금수산 일대의 군집구조를 파악하여 생태적 보전과 관리의 기초자료제공 목적으로 수행되었다. 2016년 6월부터 2016년 11월까지 식물사회학적으로 식생조사를 하였으며, 41개소의 방형구를 설정하여 식생유형분류, 중요치, 일치법을 적용하여 분석하였다. 산림식생 분석결과, 신갈나무군락군에서 군락단위로 층층나무군락, 밤나무군락(식생단위 3), 굴참나무군락(식생단위 4), 신갈나무전형군락(식생단위 5)으로 분류되었으며, 층층나무군락은 느릅나무군(식생단위 1)과 담쟁이덩굴군(식생단위 2)으로 세분되었다. 평균상대중요치는 식생단위 1에서 물푸레나무 14.9%, 산뽕나무 8.7%, 고로쇠나무 8.3% 순으로 나타났고, 식생단위 2는 일본잎갈나무 23.2%, 층층나무 20.1%, 담쟁이덩굴 6.5% 순으로 나타났다. 식생단위 3은 신갈나무 15.8%, 밤나무 13.4%, 물푸레나무 9.8% 순으로 나타났고, 식생단위 4는 신갈나무 26.6%, 굴참나무 20.8%, 소나무 16.6% 순으로 나타났으며, 식생단위 5는 신갈나무 48.3%, 쪽동백나무 7.5%, 물푸레나무 5.3% 순으로 나타났다. 일치법 분석의 결과로서 식생단위는 해발 700m, 경사 $20^{\circ}$, 지형은 사면중, 암석노출도 20%, 출현종수 30종, 교목층 식피율 80%, 교목층 수고 20m를 기준으로 분류되었다. 따라서 금수산 일대의 생태학적이고 지속가능한 산림식생관리를 위해서는 분류되어진 식생단위에 따라 차별화된 관리방법의 도출이 필요할 것으로 판단되었다.