• Title/Summary/Keyword: the coincidence method

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Multi-Epitope Fusion Protein Eg mefAg-1 as a Serodiagnostic Candidate for Cystic Echinococcosis in Sheep

  • Tianli, Liu;Xifeng, Wang;Zhenzhong, Tian;Lixia, Wang;Xingxing, Zhang;Jun, Qiao;Qingling, Meng;Shasha, Gong;Ying, Chen;Xuepeng, Cai
    • Parasites, Hosts and Diseases
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    • v.57 no.1
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    • pp.61-67
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    • 2019
  • Cystic echinococcosis (CE) in sheep is a hazardous zoonotic parasitic disease that is caused by Echinococcus granulosus (Eg). At present, serological test is an important diagnostic method for Eg infection in domestic animals. Here, a fusion protein Eg mefAg-1 harboring 8 dominant B-cell epitopes of Eg such as antigen B, tetraspanin 1, tetraspanin 6, reticulon and Eg95 was produced in E. coli and evaluated for CE in sheep by indirect ELISA. Eg mefAg-1 showed in ELISA a high sensitivity (93.41%) and specificity (99.31%), with a coincidence rate of 97.02%. Overall, it is suggested that the Eg mefAg-1 could be a potential antigen candidate for CE serodiagnosis in sheep.

Thermo-elastic analysis of rotating functionally graded micro-discs incorporating surface and nonlocal effects

  • Ebrahimi, Farzad;Heidar, Ebrahim
    • Advances in aircraft and spacecraft science
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    • v.5 no.3
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    • pp.295-318
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    • 2018
  • This research studies thermo-elastic behavior of rotating micro discs that are employed in various micro devices such as micro gas turbines. It is assumed that material is functionally graded with a variable profile thickness, density, shear modulus and thermal expansion in terms of radius of micro disc and as a power law function. Boundary condition is considered fixed-free with uniform thermal loading and elastic field is symmetric. Using incompressible material's constitutive equation, we extract governing differential equation of four orders; to solution this equation, we utilize general differential quadrature (GDQ) method and the results are schematically pictured. The obtained result in a particular case is compared with another work and coincidence of results is shown. We will find out that surface effect tends to split micro disc's area to compressive and tensile while nonlocal parameter tries to converge different behaviors with each other; this convergence feature make FGIMs capable to resist in high temperature and so in terms of thermo-elastic behavior we can suggest, using FGIMs in micro devices such as micro turbines (under glass transition temperature).

Estimation of Submarine Groundwater Discharge in Il-Gwang Watershed Using Water Budget Analysis and Rn Mass Balance (물 수지 방법과 라돈 물질수지 방법을 이용한 일광유역의 해저용출수 평가)

  • Gwak, Yong-Seok;Kim, Sang-Hyun;Lee, Yong-Woo;Hamm, Se-Yeung;Kim, In-Soo;Khim, Boo-Keun
    • Journal of Environmental Science International
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    • v.20 no.9
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    • pp.1165-1182
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    • 2011
  • The evaluation of potential submarine groundwater is an important research topic for exploring an alternative water resource. Two different approaches, water budget analysis and Rn mass balance method, were employed to investigate the annual variation of submarine groundwater discharge in 2010 at a marine watershed located at the south-eastern part of Korean Peninsula. In order to obtain reliable hydrological data during study period, temporal and spatial variations of rainfall and soil moisture had been collected and hydro-meterological data such as temperature, humidity and wind speed were collected The runoff response was simulated using SCS-CN method with spatial distributions of landuse and soil texture from GIS analysis. Six different methods were used to estimate the monthly variation of evapotranspiration and field measurements of soil moisture were used to account for the infiltration. Comparisons of infiltration and surface runoff between simulation and water balance with measurements showed coincidence. The water budget analysis and Rn mass balance method provide mean daily submarine groundwater as 5.35 and 4.07 $m^3/m/day$ in 2010, respectively.

The Fingerprinting of Huangjinju Powder for Injection on Chinese Patent Medicine by XRD Fourier

  • Pan, Yan-Li;Zhang, Gui-Jun;Gong, Ning-Bo;Wu, Yun-Shan;Lu, Yang;Luo, Rong;Choi, Ho-Young
    • Korean Journal of Medicinal Crop Science
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    • v.14 no.2
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    • pp.117-123
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    • 2006
  • The purpose is to study the identification method of Huangjinju powder for injection and the medicinal materials by the fingerprint off-ray Diffraction Fourier (XRDF). We used the same method on both the studying of Huangjinju and the medicinal materials. Then we selected a few components alignment to compare. We analyzed the data by setting up the deviation $d({\AA})$ as ${\pm}0.05$ to calculate the rate of special mark on the sample (Px) and on the patent (P). The special XRDF of Huangjinju$[d({\AA})/(I/I_0)]$ have 5 peaks that have not expressed in medicinal materials. Therefore Px is 22.73%. Flos Trollii Chinensis has 3 special marks and Px is 17.65%. Flos Chrysanthemi Indici has 1 special mark and Px is 3.57%. Its coincided interplanar spacing with the patent is $2.907{\AA}$. Flos Lonicerae Japonicae has 6 special marks and Px is 23.08%. Its special mark in the patent are 4.95/14 and 4.50/15, respectively. The P is 9.09%. Its coincided interplanar spacing with the patent is $2.910{\AA}\;and\;3.05{\AA}$, respectively. The number of special XRDF mark peaks of baicalin is 9 and Px is 18.37%. Its coincided interplanar spacing with the patent is $2.910{\AA}$. It has visible mark and specificity adopting XRDF fingerprint to identify Huangjinju and medicinal materials. Establishing the quality standard is a synthetic index that depends both on special marks in the medicinal materials of the patent and on the coincidence peak data.

Optimized TOF-PET detector using scintillation crystal array for brain imaging

  • Leem, Hyuntae;Choi, Yong;Jung, Jiwoong;Park, Kuntai;Kim, Yeonkyeong;Jung, Jin Ho
    • Nuclear Engineering and Technology
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    • v.54 no.7
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    • pp.2592-2598
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    • 2022
  • Research groups in the field of PET instrumentation are studying time-of-flight(TOF) technology to improve the signal-to-noise ratio of PET images. Scintillation light transport and collection plays an important role in improving the coincidence resolving time(CRT) of PET detector based on a pixelated crystal array. Four crystal arrays were designed by the different optical reflection configuration such as external reflectors and surface treatment on the CRT and compared with the light output, energy resolution and CRT. The design proposed in the study was composed of 8 × 8 LYSO crystal array consisted of 3 × 3 × 15 mm3 pixels. The entrance side was roughened while the other five surfaces were polished. Four sides of all crystal pixels were wrapped with ESR-film, and the entrance surface was covered by Teflon-tape. The design provided an excellent timing resolution of 210 ps and improved the CRT by 16% compared to the conventional method using a polishing treatment and ESR-film. This study provided a method for improving the light output and CRT of a pixelated scintillation crystal-based brain TOF PET detector. The proposed configuration might be an attractive detector design for TOF brain PET requiring fast timing performance with high cost-effectiveness.

Modeling of the Power/Ground Plane Noise Including Dielectric Substrate Loss (유전체 손실을 고려한 전원부에서 유기되는 노이즈 모델링에 관한 연구)

  • Kim, Jong-Min;Nam, Ki-Hoon;Ha, Jung-Rae;Song, Ki-Jae;Na, Wan-Soo
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.21 no.2
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    • pp.170-178
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    • 2010
  • In this paper, we propose the modeling of the power/ground plane which includes complex dielectric permittivity and loss tangent for the power/ground coupled noise. In order to estimate the effects of the dielectric substrate for the coupled noise, we used full-wave simulators, HFSS(High Frequency Structure Simulation) and MWS(MicroWave Studio). The simulated results for the commercial substrates are compared with the measured values. TLM(Transmission Line Method) was used for the calculation of power plane impedance using Debye model which depicts the dielectric loss of PCB. Finally, impedance from proposed circuit model showed very good coincidence to the measured data.

Establishment of Neospora agglutination test for serologic diagnosis of neosporosis (Neospora응집반응을 이용한 네오스포라증의 혈청학적 진단)

  • Kang, Min-Soo;Kim, Jae-Hoon;Hwang, Woo-Suk;Nam, Ho-Woo;Youn, Hee-Jeong;Bae, Jong-Hee;Kim, Dae-Yong
    • Korean Journal of Veterinary Research
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    • v.43 no.4
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    • pp.677-681
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    • 2003
  • Currently, both the indirect fluorescent antibody test (IFAT) and enzyme linked immunosorbent assay (ELISA) have been used to detect Neospora caninum antibodies. Several factors such as the buffers, the conjugate, the pattern of fluorescence, and the cross reactivity with other apicornplexan protozoan, may result in poorly correlated data. The present study was undertaken to develop and evaluate the Neospora agglutination test (NAT) for the detection and quantification of IgG antibodies to N. caninum from various animal species. Compared to the ELISA method, the NAT with a cutoff value of 1:512 gave a high index of coincidence (kappa=0.807) and no cross reactivity to Toxoplasma gondii antiserum. Hence, this NAT method, which did not require a species-specific secondary antibody and expensive tools, would be easily available for the detection of antibodies to N. caninllm of various animal species.

Classification of Archaebacteria and Bacteria using a Gene Content Tree Approach (Gene Content Tree를 이용한 Archaebacteria와 Bacteria 분류)

  • 이동근;김수호;이상현;김철민;김상진;이재화
    • KSBB Journal
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    • v.18 no.1
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    • pp.39-44
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    • 2003
  • A Gene content phylogenetic tree and a 16s rRNA based phylogenetic tree were compared for 33 whole-genome sequenced procaryotes, neighbor joining and bootstrap methods (n=1,000). Ratio of conserved COG (clusters of orthologous groups of proteins) to orthologs revealed that they were within the range of 4.60% (Mezorhizobium loti) or 56.57% (Mycopiasma genitalium). This meant that the ratio was diverse among analyzed procaryotes and indicated the possibility of searching for useful genes. Over 20% of orthologs were independent among the same species. The gene content tree and the 16s rDNA tree showed coincidence and discordance in Archaeabacteria, Proteobacteria and Firmicutes. This might have resulted from non-conservative genes in the gene content phylogenetic tree and horizontal gene transfer. The COG based gene content tree could be regarded as a midway phylogeny based on biochemical tests and nucleotide sequences.

Development of Sandwich ELISA for the Detection of Shrimp in Processed Foods (가공식품 중 새우의 검출을 위한 샌드위치 ELISA의 개발)

  • Do, Jeong-Ryong;Back, Su-Yeon;Shon, Dong-Hwa
    • Korean Journal of Food Science and Technology
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    • v.46 no.5
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    • pp.538-543
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    • 2014
  • A sandwich enzyme-linked immunosorbent assay method (sELISA) for detecting the presence of shrimp in processed foods was developed using rabbit polyclonal antibodies against tropomyosin produced by black tiger prawns (shrimp). Based on the standard curve derived using this method, the detection range of shrimp was determined to be $1-100{\mu}g/mL$. The cross-reactivity of these antibodies toward black tiger prawns, fleshy prawns, cocktail prawns, lobster, and blue crab was 100, 73, 155, 18, and 0%, respectively. When shrimp was heated for 10 min, the mean assay recovery of tropomyosin was 121-221% at $70-100^{\circ}C$ and 7.8% at $121^{\circ}C$. When shrimp was added to cream soup, weaning food, sausage, fish paste, and sauce, the mean assay recovery was 397, 639, 168, 234, and 0%, respectively. In sample tests involving 14 commercial items, the coincidence ratio of assay results and reference was 79%.

Community Structure of Forest Vegetation in Mt. Geumsusan belong to Woraksan National Park (월악산국립공원 금수산 산림식생의 군집구조)

  • Kim, Ho-Jin;Shin, Jae-Kwon;Lee, Cheol-Ho;Yun, Chung-Weon
    • Korean Journal of Environment and Ecology
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    • v.31 no.2
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    • pp.202-219
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    • 2017
  • The purpose of this study was to analyze the community structure of forest vegetation in Mt. Geumsusan belong to Woraksan National Park for providing basic information of ecological management. Data were collected by 41 plots from June to November in 2016 using Z-M phytosociology method, which was analyzed with vegetation types, mean importance value and coincidence method. As the results, the forest vegetation was classified into Quercus mongolica community group including Cornus controversa community, Castanea crenata community(Vegetation unit 3), Quercus variabilis community(Vegetation unit 4) and Q. mongolica community(Vegetation unit 5). C. controversa community was divided into two groups such as Ulmus davidiana var. japonica group(Vegetation unit 1) and Parthenocissus tricuspidata group(Vegetation unit 2). Mean importance percentage of vegetation unit 1 was showing Fraxinus rhynchophylla 14.9%, Morus bombycis 8.7% and Acer pictum subsp. mono 8.3%, that of unit 2 was Larix kaempferi 23.2%, C. controversa 20.1% and P. tricuspidata 6.5%, that of unit 3 was Q. mongolica 15.8%, C. crenata 13.4% and F. rhynchophylla 9.8%, that of unit 4 was Q. mongolica 26.6%, Q. variabilis 20.8% and Pinus densiflora 16.7%, that of unit 5 was Q. mongolica 48.3%, Styrax obassia 7.5%, F. rhynchophylla 5.3% in the order, respectively. Each vegetation unit was classified with dependance on environmental factors as 700m of altitude, $20^{\circ}$ of slope degree, middle slope of topography, 20% of bare rock, 30 taxa of present species, 80% of tree layer coverage rate and 20m of tree layer height. In conclusion, it was preferentially considered that development of peculiar and specific management methods with vegetation unit classified above should be needed for ecological and sustainable forest vegetation management.