• Title/Summary/Keyword: the Salmons

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Genetic Identification of the North Pacific Chum Salmon (Oncorhynchus keta) Stocks (유전적 형질에 의한 북태평양 연어 (Oncorhynchus keta)의 계군 구분)

  • JUNG Woongsic;LEE Youn-Ho;KIM Suam;JIN Deuk-Hee;SEONG Ki Baek
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.36 no.6
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    • pp.578-585
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    • 2003
  • The chum salmon (Oncorhynchus keta) is an anadromous fish distributed all around the North Pacific. Artificial production and release of the juveniles are being made by Korea, Japan, Russia, Canada and the United States. It is important to set up some criteria identifying each stock in order to clarify each nation's right of harvest for the chum salmon resource. As an attempt to build such criteria, we analyzed sequences of a microsatellite DNA Ogo5 and the COIII-ND3-ND4L region of the mitochondrial DNA from chum salmons of Korea, Japan, and the United States. Ogo5 has 4 different alleles: allele A, B-1, B-2, and B-3. Allele B-3 is found only in 3 individuals out of 12 Korea salmons. The Japan salmons have the other 3 alleles and the America salmons have only 2 allots, A and B-1. Heterozygosity index (Ho/He) distinguishes the Korea (1.61) and Japan salmons (1.63) from the America ones (1.09). Seventeen different haplotypes are found in the COIII-ND3-ND4L region from 60 individuals,20 from each stock. The gene genealogy of the haplotypes revealed by TCS program shows that the Korea and Japan salmons are genetically closely linked, but that they are clearly distinguished from the America ones. Ten and eleven individuals of the Korea and Japan salmons have an identical haplotype. Nine individuals of the Korea salmons $(45\%),$ however, are separable from the Japan salmons by their own specific nucleotides. This result presents usefulness of the COIII-ND3-ND4L region as a genetic marker for identification of the chum salmon stocks.

Genetic Similarity-dissimilarity Among Korea Chum Salmons of Each Stream and Their Relationship with Japan salmons (한국 연어의 소상하천간 유전적 유사성과 차이점 및 일본 연어와 유전적 관계)

  • Kim, Go-Eun;Kim, Choong-Gon;Lee, Youn-Ho
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.12 no.2
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    • pp.94-101
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    • 2007
  • Analysis of population structure of Oncorhynchus keta, the most abundant salmon in the East Sea of Korea, has not been much carried out despite its importance as a fishery resource in the North Pacific. Currently, molecular methods are being applied to stock identification and a method of using single nucleotide polymorphisms (SNPs) is getting more popular. In this study, we analyzed the 720 bp long sequence of the mtDNA COIII-ND3-ND4L region in order to examine genetic similarity-dissimilarity among the Korea chum salmons of each stream and their relationship with the Japan chum salmons. A total of 152 individuals were analyzed, 108 from 3 locations of Korea and 44 from 2 locations of japan, which resulted in as many as 29 different haplotypes. Pairwise $F_{ST}$ and AMOVA tests of the populations show that there is no significant population-level genetic difference among the chum salmons analyzed ($F_{ST}<0.07$). On the other hand, haplotype relationships among the individuals reveal that approximately 25% of the Korea salmons consist genetic lineages independent of Japan salmons and also that a genetic lineage exists in the Puk river and the Namdae river salmons independent of the Wangpi river salmons of Korea.

DNA Heteropolymorphism of Chum Salmon Detected by Denaturing Gradient Gel Electrophoresis and Real Time PCR (Denaturing gradient gel electrophoresis와 real time PCR 방법을 이용한 연어 유전자들의 DNA 이형 다양성 검색)

  • Ham Seung Hub;Lee Suk Keun;Han Hyon Sob;Jin Deuk Hee
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.35 no.5
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    • pp.490-496
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    • 2002
  • In order to detect the DNA heteropolymorphism of chum salmon, selected essential genes were examined in different regional chum salmons, i.e., Korean, Japanese and American by denaturing gradient gel electrophoresis (DGGE) and real time PCR methods. From the promoter regions and introns of growth hormone, mtDNA NDI region, D-loop region, IGF-I, histone H3 and MCH2 several representative primer pairs were obtained and employed for the DGGE with the PCR products from the genomic DNAs of the different regional chum salmons. mtDNA NDI, D-loop region and IGE-I genes showed marked heteropolymorphism between Korean and American chum salmons. Intron C of growth hormone also showed a heteropolymorphism between Korean and Japanese chum salmons. Whereas heteropolnnorphism of histone liH and MCH2 genes was detected among in Korean, Japanese and Asnerican chum salmons in the examined region. The real time PCR disclosed the characteristic incremental production of target DNAs dependent on the heteropolymorphic conditions of genomic DNAa of chum salmons, thus the different regional chum salmons could be grouped by the variable incremental curies. Although the DGGE and real time PCR did not produce the identical results in this study, we suggest that the DGGE and real time PCR could be used for the primary screening of the DNA heteropolymorphism of different animal genome.

The Distribution and Migration Boundary Lines of Oncorhynchus keta in the Milyang River (연어(Oncorhynchus keta)의 밀양강 분포 및 소상 한계선 파악)

  • Hong, Donghyun;Seong, Ki Baik;Ko, Eui-Jeong;Jung, Eunsong;Jo, Hyunbin;Joo, Gea-Jae
    • Korean Journal of Ecology and Environment
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    • v.53 no.4
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    • pp.445-452
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    • 2020
  • In this study, we carried out a distribution and migration boundary lines of chum salmon (Oncorhynchus keta) in the Milyang River. We measured the total length of dead chum salmons. As a results, 40 chum salmons were found during the study period, 25 dead salmons and 15 alive individuals. The ratio of female was 64%. We verified the migration boundary lines of chum salmons based on a sighting survey until detecting a structure that chum salmons are not able to migrate upstream. We discovered that chum salmons were not able to migrate up to 13 km in a mainstream of the Milyang River and up to 12 km in the Danjang stream (a tributary of the Milayang River) from upstream of the Yerim Bridge. Therefore, our results indicate that Milyang River should improve a river connectivity by demolishing weirs that disturb chum salmon's migration or installing appropriate fishways. Also, effective legislations are needed to retain naturality in spawning ground and micro-habitats to increase the survival rate of parrs and smolts.

Cloning and DNA Sequences Anaylsis of Mitochondrial NADH Dehydrogenase Subunit 3 from Korean Chum Salmon, Oncorhynchus keta (한국산 연어의 미토콘드리아 NADH Dehydrogengse Subunit 3 영역의 클로닝 및 DNA 염기서열 분석)

  • CHOI Yoon-Sil;LEE Youn-Ho;JIN Deuk-Hee
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.36 no.2
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    • pp.94-99
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    • 2003
  • Mitochondrial DNAs has been used frequently as genetic markers for the population genetic studies of salmonid fishes. Samples used in this experiment were chum salmons (Oncorhynchus keta) from Korea. We analyzed variation of mitochondrial NADH dehydrogenase subunit 3 gene (ND3) among 4 individuals of the Korea population. Genomic DNA was extracted from the liver of the chum salmon samples. Then, the ND3 gene was amplified by polymerase chain reaction (PCR) including the 3' region of cytochrome oxidase III gene (COIII) and the 5` region of NADH dehydrogenase subunit 4L gene (ND4L). The size of the PCR product was 752 Up and the sequences showed some genetic variation among those four individuals. Genetic variations were observed in 7 sites as single nucleotide polymorphism (SNP). Within the open reading frame of the ND3 gene which encodes 116 amino acids, 5 nucleotide substitutions were found. Both transitional and transversional changes occurred more frequently with transitional changes. Comparison of these sequences with the others of a Japanese chum salmon in GenBank showed 5 sites of SNPs. This study provided the basic information of SNP in ND3 gene among Korean chum salmons and demonstrated the possible use of the SNP data as a genetic marker.

Reconnaissance study on the Water Quality of Miomotegawa River for the evaluation of the impacts of acid pollution

  • Yang, Hyung-Jae;Satake, Kenichi
    • Proceedings of the Korean Sanitation Conference
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    • 2006.11a
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    • pp.33-42
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    • 2006
  • We decided Miomotegawa river was the object of our investigation since it is one of the most important salmon rivers in Japan. A great number of salmonshave returned to the river, and it was expected to contain relatively lower cation concentrations since granite is widely distributed in the river catchment area. The concentration of calcium and magnesium were less than a half of that of other major rivers. The concentration of analyzed parameters indicating water quality effect on salmons, $NO_{3},\;SO_{4}\;and\;NH_{4}$, were 1.37, 4.08 and 0.01mg/l, respectively. Most parameters down stream were greater in concentration than in the upper stream.

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Reconnaissance Study for the Impact Evaluation of Acid Pollution at Miomotegawa River

  • Yang, Hyung-Jae;Park, Jung-Min
    • Journal of environmental and Sanitary engineering
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    • v.23 no.4
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    • pp.31-36
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    • 2008
  • This study was implemented to investigate the anion and cation concentrations of Miomotegawa river since it is one of the most important salmon rivers in Japan. A great number of salmons have returned to the river, and it was expected to contain relatively lower cation concentrations since granite is widely distributed in the river catchment area. The anion and cation concentrations of station 1 except $NH_4{^+}$ were higher than those of other stations. The concentrations of $Na^+,\;K^+,\;Mg^{2+},\;Ca^{2+}$, and $Cl^-$ at station 2 were 8.61, 1.06, 1.84, 4.47, and 14.5mg/l, respectively. In the case of $NH_4{^+},\;NO_3{^-}$ and ${SO_4}^{2-}$, they were 0.04, 2.99 and 5.31mg/l, respectively at station 2 of down stream. However, at station 5 of upstream, they were 0.01, 1.37 and 4.08mg/l, respectively, which were relatively lower than that of downstream.

Morphological study of Oncorhynchus spp.(Pisces : Salmonidae) in Korea- IV. Comparison of morphological characters of chum salmon Oncorhynchus keta, masu salmon, Oncorhynchus masou and rainbow trout Oncorhynchus mykiss (한국산 연어속 어류의 형태학적 연구- IV. 연어, Oncorhynchus keta, 산천어 Oncorhynchus. masou, 및 무지개송어 Oncorhynchus. mykiss의 형태 비교)

  • Myoung, Jung-Goo;Kim, Yong-Uk
    • Korean Journal of Ichthyology
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    • v.5 no.1
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    • pp.96-112
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    • 1993
  • The morphological characteristics of chum salmon Oncorhynchus keta, masou salmon. Oncorhynchus masou and rainbow trout, Oncorhynchus mykiss in Korea were studied from 1989 to 1990. It was found that 13 factors of the female and 11 factors of the male in body form, number of pyloric ceaca and gill rakers has significant specific differences. There were some differences among species in the ratios of body depth, caudal peduncle depth, dorsal fin length and anal fin length to standard length (PBL), and snout length, eye diameter, upper jaw length, and cheek to head length. In the ratio of head length to fork length of the male, chum salmon showed the highest(24.7%) value and rainbow trout the lowest(21.6%). No difference was found in the female. It was proved that number of vertebrae, gill rakers, pyloric ceaca and lateral line scales were useful taxonomical characteristics of the salmon species. In particular, the ratio of eye diameter (ED) to head length (HL) appeared to be a new taxonomic criterion for the salmons in Korean waters.

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Morphological Study of Oncorhynchus spp. in Korea-V. Comparison of Skeletal Characters of Chum Salmon O. keta, Masu Salmon O. masou and Rainbow Trout O. mykiss (한국산 연어속 어류의 형태학적 연구-V. 연어 (Oncorhynchus keta), 산천어 (O. masou) 및 무지개송어 (O. mykiss)의 골격 비교)

  • MYOUNG Jung-Goo;KIM Yong Uk
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.29 no.2
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    • pp.208-229
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    • 1996
  • Comprative osteology of the chum salmon Oncorhynchus keta, masu salmon O. masou and rainbow trout O. mykiss in Korea was studied. As a result, it was found that there were some differences in the structural features of the cranium and visceral skeleton among the species. Interspecific differencies in glossohyal, supraethmoid, parasphenoid and vomer were distinctive for the species. Chum salmon could be distinguished easily by some characters on form of glossohyal which is thin, long and sharp in shape. It has triangle shape supraethmoid while other two species have oval shape one. The shape of parasphenoid, preopercle, hyomandibular and the number of epurals were evaluated as new taxonomic criteria for the salmons.

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A Study on the Current Situation of Adult Children Cohabiting with Their Parents and an Exploration of the Frame of Analysis (성인자녀의 부모 동거 현황 및 분석틀의 탐색)

  • Choi, Youn Shil
    • Journal of Families and Better Life
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    • v.32 no.4
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    • pp.75-89
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    • 2014
  • The purpose of this study is to explore whether the phenomenon of both married and unmarried adults' cohabiting with their parents in Korean society is "unilaterally parasitic" on the child's side, or is "interdependent", characterized by expectation and dependence from the parent's side. As a result of this study possessing the characteristics of theoretic research, the following propensities of parent-dependent adults have been discussed. First of all, it was discovered that the ratio of adult children dependent on and cohabiting with their parents is considerable. Second, parents cohabiting with their adult children have unfavorable sociological features, such as high age, low level of education and income, and lower standards of education and income, compared to parents in normal households. Third, it was found that parent-dependent adults in Korean society maintain a relatively high rate of financial activity and stable employment-based occupation status. Fourth, it was shown that the level of satisfaction on the relationship between parent-dependent adults and their parents was discovered to be high, which is contrastive to the prediction of negative results based on some previous researches. Single adult children's age, their level of education and financial activity status, and their parents' age and level of education were deducted as variables related to the level of satisfaction of the relationship between parents and their children. It seems that the issue of married and unmarried adults' cohabiting with their parents in Korean society should be approached from various perspectives such as political, economic, socio-cultural and developmental aspects. On the basis of this fundamental awareness and several of the materials, it is pertinent that approaches to both married and unmarried adults' cohabiting with parents in Korean society should be distinguished from approaches to those in Japanese or Western society because it reflects the uniqueness of Korean society. In the phenomenon of married and unmarried adults' cohabiting with their parents in Korean society, there are several factors besides the economic factor, especially the socio-cultural factor that have the characteristics of mutual dependence between parents and their children rather than those of unilateral parasitism, in contrast with the phenomenon in Japanese or Western society. This research was aimed to contribute by establishing basic data for policy making by providing necessary information to treat the issues of instability and anxiety related to families and reflection on the matters of generations and parent-child relationships in current Korean society.