Browse > Article
http://dx.doi.org/10.5657/kfas.2003.36.2.094

Cloning and DNA Sequences Anaylsis of Mitochondrial NADH Dehydrogenase Subunit 3 from Korean Chum Salmon, Oncorhynchus keta  

CHOI Yoon-Sil (Faculty of Marine Bioscience & Technology, Kangnung National University)
LEE Youn-Ho (Laboratory of Polar Science, Korea Ocean Research & Development institute)
JIN Deuk-Hee (Faculty of Marine Bioscience & Technology, Kangnung National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.36, no.2, 2003 , pp. 94-99 More about this Journal
Abstract
Mitochondrial DNAs has been used frequently as genetic markers for the population genetic studies of salmonid fishes. Samples used in this experiment were chum salmons (Oncorhynchus keta) from Korea. We analyzed variation of mitochondrial NADH dehydrogenase subunit 3 gene (ND3) among 4 individuals of the Korea population. Genomic DNA was extracted from the liver of the chum salmon samples. Then, the ND3 gene was amplified by polymerase chain reaction (PCR) including the 3' region of cytochrome oxidase III gene (COIII) and the 5` region of NADH dehydrogenase subunit 4L gene (ND4L). The size of the PCR product was 752 Up and the sequences showed some genetic variation among those four individuals. Genetic variations were observed in 7 sites as single nucleotide polymorphism (SNP). Within the open reading frame of the ND3 gene which encodes 116 amino acids, 5 nucleotide substitutions were found. Both transitional and transversional changes occurred more frequently with transitional changes. Comparison of these sequences with the others of a Japanese chum salmon in GenBank showed 5 sites of SNPs. This study provided the basic information of SNP in ND3 gene among Korean chum salmons and demonstrated the possible use of the SNP data as a genetic marker.
Keywords
Mitochondrial DNA; Chum salmon; COIII; ND3; ND4L; PCR; SNP;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Domanico, M.J. and R.B. Phillips. 1995. Phylogenetic analysis of Pacific Salmon (Genus Oncorhynchus) based on mitochondrial DNA sequence data. Mol. Phylo. Evol, 4, 366-371   DOI   ScienceOn
2 Hong, K.P., J.G. Myoung, J.K. Son and C.W. Park. 1994. A biochemical study for the development of genetic marker on Salmonids in Korea. J. Kor. Fish. Soc.,27(1), 83-88
3 Hwang, G.L., Y.C Lee and C.S. Chang. 1997. Mitochondrial DNA analysis of the Fleshy Prawn (Penaeus chinensis) for stock discrimination in the Yellow Sea. J.Kor. Fish. Soc., 30(I), 88-94
4 Kim, S.H., M.S. Park, Y.H. Kim and D.W. Park. 1997. Genetic analysis of mitochondrial DNA from Korea Oysters, Crassostrea gigas. J. Kor. Fish. Soc., 30(5), 804-808
5 Kim, I.S., B.Y. Min, M.H. Yoon and D.H. Kim. 1999. Mitochondrial DNA polymorphism of the Blue Mussel (MytiJus edulis) species complex on the East Coast of Korea. Kor. J. Life Sci., 9(3), 262-267
6 Lee, H.J., J.Y. Park, J.H. Lee, K.S. Min, I.G. Jeon, M.A. Yoo and W.H. Lee. 2000. Phylogeny of the subfamily Salmoninae distributed in Korea based upon nucleotide sequences of mitochondrial ribosomal RNA genes. J. Kor. Fish. Soc., 33(2), 103-109
7 McKay, S.J., R.H. Devlin and M.J. Smith. 1996. Phylogeny of Pacific salmon and trout based on growth hormone type-2 and mitochondrial NADH dehydrogenase subunit 3 DNA sequences. Can. J. Fish. Aquat. Sci., 53, 1165-1176   DOI   ScienceOn
8 McKay, S.J., M.J. Smith and R.H. Devlin. 1997. Polymerase chain reaction-based species identification of salmon and coastal trout in British Columbia. Mol. Mar. BioI. Biotechnol., 6, 131-140
9 Park, J.Y. and Y. Kim. 1995. The number of nucleotide substitutions per sites of mitochondrial DNA in the four Pleuronectid species. J. Kor. Fish. Soc., 28(5),649-658
10 Brown, W.M., J.R. George and A.C Wilson. 1979. Rapid evolution of animal mitochondrial DNA. Proc. Natl. Acad. Sci. USA, 76, 1969-1971
11 Brown, W.M., E.M. Prager, A Wang and A.C Wilson. 1982. Mitochondrial DNA sequences of primates: Tempo and mode of evolution. J. Mol. Evol., 18, 225-239   DOI
12 Verspoor, E., E.M. McCarthy and D. Knox. 1999. The phylogeography of European Atlantic salmon (Salmo salar L.) based on RFLP analysis of the ND1/16sRNA region of the mtDNA. Bio. J. Lin. Soc., 68, 129-146   DOI
13 Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laborator Manual, 2nd ed. Cold Spring Harbor Laboratory Press, New York, 545 pp