Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Cloning and DNA Sequences Anaylsis of Mitochondrial NADH Dehydrogenase Subunit 3 from Korean Chum Salmon, Oncorhynchus keta

한국산 연어의 미토콘드리아 NADH Dehydrogengse Subunit 3 영역의 클로닝 및 DNA 염기서열 분석

  • CHOI Yoon-Sil (Faculty of Marine Bioscience & Technology, Kangnung National University) ;
  • LEE Youn-Ho (Laboratory of Polar Science, Korea Ocean Research & Development institute) ;
  • JIN Deuk-Hee (Faculty of Marine Bioscience & Technology, Kangnung National University)
  • 최윤실 (강릉대학교 해양생명공학부) ;
  • 이윤호 (한국해양연구원 극지연구본부) ;
  • 진덕희 (강릉대학교 해양생명공학부)
  • Published : 2003.04.01
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Abstract

Mitochondrial DNAs has been used frequently as genetic markers for the population genetic studies of salmonid fishes. Samples used in this experiment were chum salmons (Oncorhynchus keta) from Korea. We analyzed variation of mitochondrial NADH dehydrogenase subunit 3 gene (ND3) among 4 individuals of the Korea population. Genomic DNA was extracted from the liver of the chum salmon samples. Then, the ND3 gene was amplified by polymerase chain reaction (PCR) including the 3' region of cytochrome oxidase III gene (COIII) and the 5` region of NADH dehydrogenase subunit 4L gene (ND4L). The size of the PCR product was 752 Up and the sequences showed some genetic variation among those four individuals. Genetic variations were observed in 7 sites as single nucleotide polymorphism (SNP). Within the open reading frame of the ND3 gene which encodes 116 amino acids, 5 nucleotide substitutions were found. Both transitional and transversional changes occurred more frequently with transitional changes. Comparison of these sequences with the others of a Japanese chum salmon in GenBank showed 5 sites of SNPs. This study provided the basic information of SNP in ND3 gene among Korean chum salmons and demonstrated the possible use of the SNP data as a genetic marker.

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