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http://dx.doi.org/10.5657/kfas.2003.36.6.578

Genetic Identification of the North Pacific Chum Salmon (Oncorhynchus keta) Stocks  

JUNG Woongsic (Polar Sciences Laboratory Korea Ocean Research &, Development Institute)
LEE Youn-Ho (Polar Sciences Laboratory Korea Ocean Research &, Development Institute)
KIM Suam (Department of Marine Biology, Pukyong National University)
JIN Deuk-Hee (Faculty of Marine Bioscience & Technology, Kangnung National University)
SEONG Ki Baek (Yang Yang Inland Fisheries Research Institute)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.36, no.6, 2003 , pp. 578-585 More about this Journal
Abstract
The chum salmon (Oncorhynchus keta) is an anadromous fish distributed all around the North Pacific. Artificial production and release of the juveniles are being made by Korea, Japan, Russia, Canada and the United States. It is important to set up some criteria identifying each stock in order to clarify each nation's right of harvest for the chum salmon resource. As an attempt to build such criteria, we analyzed sequences of a microsatellite DNA Ogo5 and the COIII-ND3-ND4L region of the mitochondrial DNA from chum salmons of Korea, Japan, and the United States. Ogo5 has 4 different alleles: allele A, B-1, B-2, and B-3. Allele B-3 is found only in 3 individuals out of 12 Korea salmons. The Japan salmons have the other 3 alleles and the America salmons have only 2 allots, A and B-1. Heterozygosity index (Ho/He) distinguishes the Korea (1.61) and Japan salmons (1.63) from the America ones (1.09). Seventeen different haplotypes are found in the COIII-ND3-ND4L region from 60 individuals,20 from each stock. The gene genealogy of the haplotypes revealed by TCS program shows that the Korea and Japan salmons are genetically closely linked, but that they are clearly distinguished from the America ones. Ten and eleven individuals of the Korea and Japan salmons have an identical haplotype. Nine individuals of the Korea salmons $(45\%),$ however, are separable from the Japan salmons by their own specific nucleotides. This result presents usefulness of the COIII-ND3-ND4L region as a genetic marker for identification of the chum salmon stocks.
Keywords
Oncorhynchus keta; Salmon stock; ND3; Microsatellite DNA;
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