• Horticultural Science & Technology
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• v.28 no.6
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• pp.996-1002
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• 2010

Flow cytometry (FCM) was used to measure the ploidy level of three different sports from 'Campbell Early' ($Vitis$ $labruscana$) grape. Results of the study showed different ploidy levels. FCM analysis for 'Campbell Early' grape which contains 2C DNA diploid cells showed single peak around 35-40 while 'Kyoho' grape with 4C DNA tetraploid cells had a different level of 70-80. However, analysis of the sports displayed a histogram with 2 peaks containing both 2C and 4C nuclei. There was no difference in histograms of 2C DNA flesh and pericarp; on the other hand, 4C DNA flesh type of sports had a different histogram from that of the 2C DNA pericarp. Chromosome numbers of diploid ('Campbell Early'), tetraploid ('Kyoho'), and three sports were counted under the microscope. 'Campbell Early' and 'Kyoho' have 38 and 76 chromosomes, respectively. Three different sports are mixoploids with mixtures of diploid and tetraploid cells. Microscopic observations of shoot apical meristems in sports from 'Campbell Early' grape were carried out to determine the type of plant chimera. 'Campbell Early' grape (diploid) and 'Kyoho' grape (tetraploid) showed that both had 2 tunica layers covering corpus cells, while the three different sports had tunica layers showing mostly oblique division. Most cells from 'Kyoho' grape were larger than 'Campbell Early' grape. Cells from L-2 and L-3 layers of the three sports were similar to 'Kyoho' grape in size, although all cells in L-1 surface layer were uniform in size like 'Campbell Early' grape. Results of FCM analysis indicated that both normal and polyploid cells could be intermixed in sports and could become mixoploidy consisting of diploid and tetraploid. All sports used in the tests were periclinal chimera plants with two distinct L-1 and L-2 cell layers. The result of this study suggests that all three sports which originated from 'Campbell Early' grape might be 2-4-4 type chimera formation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.3
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• pp.394-400
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• 2015

The roots of Platycodon grandiflorum species either dried or fresh, are used as an ingredient in salads and traditional cuisine in Korea. To interpret the root proteins, a systematical and targeting analysis were carried out from diploid and tetraploid roots. Two dimensional gels stained with CBB, a total of 39 differential expressed proteins were identified from the diploid root under in vivo condition using image analysis by Progenesis Same Spot software. Out of total differential expressed spots, 39 differential expressed protein spots (${\geq}\;1.5$-fold) were analyzed using LTQ-FTICR mass spectrometry. Except two proteins, the rest of the identified proteins were confirmed as down-regulated such as Isocitrate dehydrogenase, Proteasome subunit alpha type-2-B. However, the most of the identified proteins from the explants were mainly associated with the oxidoreductase activity, nucleic acid binding, transferase activity and catalytic activity. The exclusive protein profile may provide insight clues for better understanding the characteristics of proteins and metabolic activity in various explants of the economically important medicinal plant Platycodon grandiflorum.

• Horticultural Science & Technology
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• v.31 no.3
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• pp.352-358
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• 2013

For the germination and differentiation of immature embryos obtained by artificial crossing between tetraploid grape 'Fujiminori' (Vitis vinifera ${\times}$ V. labruscana) and triploid 'Summer Black' (V. labruscana ${\times}$ V. vinifera), were incubated in vitro using MS medium supplemented with $GA_3$ or coconut water (CW) at various concentrations. The percentage of embryo formation of 'Fujiminori' ${\times}$ 'Summer Black' was 64.3%. Embryo germination percentage was higher than 95% in all the $GA_3$ treatments at the concentrations of 0.01, 0.05, 0.25, and $1.25mg{\cdot}L^{-1}$. However, only 15.8-31.6% of the germinated embryos successfully developed into normal plantlets. At higher concentration of $GA_3$, the plantlets developed infirm hypocotyls with over elongated and less enlarged structure. Among the treatments of CW at the concentrations of 5, 10, 15, and 20% (v/v), 10% and 15% were more effective and plantlet achievement percentage were 68.4 and 66.7%, respectively. The addition of 10% CW was most effective to obtain plantlets with optimal shoot length, node and root numbers. 15% CW was suitable to obtain plantlets with longer roots. Accordingly, the embryo culture using the MS medium supplemented with 10-15% CW was observed to be more efficient for germinating and growing the immature embryos produced from artificial crossing between tetraploid grape 'Fujiminori' and triploid 'Summer Black'.

• Journal of the Korean Society of Food Science and Nutrition
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• v.46 no.2
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• pp.196-201
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• 2017

In this study, antioxidant activities, proximate composition, and physicochemical characteristics of tetraploid 'Etteum' variety in Platycodon grandiflorum (SD) were evaluated and compared with those of a domestic diploid variety in P. grandiflorum (ND). Moisture content, crude fat, crude fiber, and crude ash contents were higher in ND, whereas crude protein and carbohydrate content were higher in SD. The amount of crude saponin in SD (2.01%) was higher than that in ND (0.88%). The reducing sugar contents of ND and SD were 3.09% and 2.82%, respectively (P<0.05). The pH level was lower in the ND (ND, 4.98; SD, 5.68). Acidity was 2.74% in ND and 2.34% in SD. Under the Hunter color system, redness was lower and lightness/yellowness higher in SD compared to those in ND. Total phenol contents of ND and SD were 0.100 mg/mL and 0.227 mg/mL, respectively. Antioxidant activities based on 1,1-diphenyl-2-picrylhydrazyl scavenging activity and SOD-like activity of SD were higher than those in ND. Based on these results, SD is a good candidate for food processing in terms of its physicochemical and antioxidative activities.

• Korean Journal of Plant Tissue Culture
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• v.22 no.3
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• pp.121-126
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• 1995

Diploid plants were obtained by anther culture of tetraploid poplar(Populus alba L. X P.glandutosa Uyeki). The effect 2,4D on callus formation from anther culture was greater than any other auxins tested. The highest average number of multiple shoots per callus was obtained when zeatin was used at levels of 6-8 ${\mu}$M. Regenerated shoots were excised and transferred to MS basal medium. Rooted plantlets were subsequently transferred to pots containing artificial soil mix. Finally 100 plane were transplanted in nursery located in forest Genetics Research Institute. for the 300 anther clones growing in greenhouse for 6 months after transplanting, 33% were slow-growing, 47% were rapid-growing and 20% had huge leaf size with rapid-growing characteristics. Chromosome study showed a narrow range of variation from diploid to tetraploid. DNA polymorphism studies using various RAPD markers revealed some extend of differences among the anther-clones in their band pattern.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.3
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• pp.365-370
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• 1995

This study was conducted to obtain the basic information on salinity tolerance in Italian Ryegrass. Seeds of 20 cultivars that contained diploid(9 cultivars) and tetraploid(l1 cultivars) types were exposed to eight levels of NaCl concentration ranging from 0 to 350 mM under three levels (15$^{\circ}C$, 2$0^{\circ}C$ and $25^{\circ}C$) of temperature. There were highly significant differences in germination percentage among the levels of NaCl concentration, levels of temperature and cultivars, respectively. Average germination percentage of cultivars in control(no NaCl was higher at 2$0^{\circ}C$(90.1%) than that of $25^{\circ}C$(87.8%) or 15$^{\circ}C$(87.5%). Average germination percentage of cultivars began to decrease over 200mM of NaCl concentration. The degree of decrease in germination was higher at $25^{\circ}C$ than that of 15$^{\circ}C$ or 2$0^{\circ}C$. At 2$0^{\circ}C$, the germination percentage and gemination speed were gradually decreased as NaCl concentration increased. The average germination day was lengthened by the increase of NaCl concentration and temperature. The germination percentage in diploid types were higher than that of tetraploid types but the average germination day in diploid types was shortened more than that of tetraploid types, regardless of temperature and NaCl concentration.

• Korean Journal of Environment and Ecology
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• v.25 no.5
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• pp.710-716
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• 2011

The growth characteristics and the several component content of leaves in one year old tetraploid of Robinia pseudoacacia introduced at five site planted respectively were surveyed in Sangwon area Pyongyang, and the expand plant possibility and the leaves harvested as feed additive use of possibility in Democratic People's Republic of Korea was discussed in comparison with tetraploid of R. pseudoacacia of Yanqing area. The results were as follows (1) the length and stump diameter of annual sprouting stems at Sangwon area were smaller 50% than the tetraploiod of R. pseudoacacia stems that Yanqing area; (2) the length of sprouting stems in slope sites of Eastern (E), Western(W) and Southern(S) at Sangwon were higher 23~30% than the length that Northern slope site(N), and the diameters of sprouting stem in the slope of Eastern(E) and Southern(S) were higher 14~23% than the slope sites of Northern(N) and Western(W); (3) crude protein contents and ash contents of leaves at Sangwon were higher 40% general R. pseudoacacia and similar to the leaves that Yanqing area.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 2006.05a
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• pp.186-191
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• 2006

• Journal of Plant Biology
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• v.32 no.3
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• pp.181-188
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• 1989

C-banded karyotypes in A. anisopodium, A. sacculiferum, A. deltoide-fistulosum and A. splendens were investigated. The chromosome compositions were diploid of 2n=16 in A anisopodium and A. splendens, and teiraploid of 2n=32 in a. sacculiferum and A. deltoide-fistulosum. The bands were distributed in telomeric parts of the chromosomes dominantly in addition to interstitial regions sporadically, resulting in the specific C-banded karyo types according to the species. No centromeric band was observed in these species except only one chromosome in A. deltoidefistulosum. The interspecific relationship based on the C-band distribution will be discussed.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.5
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• pp.745-753
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• 2008

Gene-manipulated mice were discovered for the first time about a quarter century ago. Since then, numerous sophisticated technologies have been developed and applied to answer key questions about the fundamental roles of the genes of interest. Functional genomics can be characterized into gain-of-function and loss-of-function, which are called transgenic and knock-out studies, respectively. To make transgenic mice, the most widely used technique is the microinjection of transgene-containing vectors into the embryonic pronucleus. However, there are critical drawbacks: namely position effects, integration of unknown copies of a foreign gene, and instability of the foreign DNA within the host genome. To overcome these problems, the ROSA26 locus was used for the knock-in site of a transgene. Usage of this locus is discussed for the gain of function study as well as for several brilliant approaches such as conditional/inducible transgenic system, reproducible/inducible knockdown system, specific cell ablation by Cre-mediated expression of DTA, Cre-ERTM mice as a useful tool for temporal gene regulation, MORE mice as a germ line delete and site specific recombinase system. Techniques to make null mutant mice include complicated steps: vector design and construction, colony selection of embryonic stem (ES) cells, production of chimera mice, confirmation of germ line transmission, and so forth. It is tedious and labor intensive work and difficult to approach. Thus, it is not readily accessible by most researchers. In order to overcome such limitations, technical breakthroughs such as reporter knock-in and gene knock-out system, production of homozygous mutant ES cells from a single targeting vector, and production of mutant mice from tetraploid embryos are developed. With these upcoming progresses, it is important to consider how we could develop these systems further and expand to other animal models such as pigs and monkeys that have more physiological similarities to humans.