• Title/Summary/Keyword: test-strip

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Optimization of disposable paper-based test strips for hypochlorous acid detection

  • Rita E. Ampiaw;Muhammad Yaqub;Changyeon Woo;Wontae Lee
    • Membrane and Water Treatment
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    • v.14 no.4
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    • pp.181-189
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    • 2023
  • The Covid-19 pandemic has increased demand for chlorine-based sanitizing solutions, most of which contain hypochlorous acid (HOCl) as an active agent. Free chlorine (HOCl) in these sanitizers is crucial for their efficacy. Disposable test strips are affordable and convenient tools for determining various qualitative and quantitative parameters. In this study, disposable opto-chemical test strips were developed by physically immobilizing 3,3',5,5'-tetramethylbenzidine (TMB) and o-dianisidine (o-D) reagents on chromatography and filter paper-based test strips for the visualization and detection of free chlorine in the form of HOCl. The reagents undergo a rapid color change upon reaction with chlorine through a redox reaction. The paper-based test strips showed rapid color change within a minute and a low sample volume requirement (1 ml). This portable, disposable paper-based test strip is a simple and cost-effective way to rapidly detect the presence of HOCl sanitizers for home and field applications. Both TMB and o-D successfully detected chlorine. Chromatography paper proved to be the more efficient option among the two papers used as substrates for the reagents (TMB and o-D). It exhibited high retention capacity and high performance in terms of color transformation when reacting with HOCl, even after two months of storage.

Effects of locally-delivered minocycline hcl on controlled periodontal disease (Minocycline 국소 약물 방출 제제의 치주질환 치료 효과)

  • Chung, Mi-Hyun;Kwon, Young-Hyuk;Herr, Yeek;Lee, Man-Sup;Park, Joon-Bong
    • Journal of Periodontal and Implant Science
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    • v.28 no.1
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    • pp.37-56
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    • 1998
  • The purpose of this study was to evaluate the clinical and microbiological outcomes following the use of 30% minocycline-loaded polycaprolacton film and 2% minocycline-loaded gel that was applied locally into pockets combined with scaling and root planing. 25 human subjects who were non-pregnant, non-lactating, aged 20-50 and diagnosed as moderate to advanced adult periodontitis were enrolled. Subjects were excluded if they had a history of severe acute or chronic systemic disease, if they required antibiotic prophylaxis for dental treatment for any reason, or if they reported a history suggestive of hypersensitivity reactions to minocycline or tetracycline. 4quadrants that had several teeth with a 5-8mm probing pocket depth and radiographic evidence of alveolar bone loss for each patient were selected and divided into test sites and control sites according to the split-mouth design. Scaling and root planing was done for each site at baseline(0week). Test sites received the minocycline gel and strip and control sites had saline irrigation. The patients received both treatments simyltaneously. Subgingival irrigation of sterile saline was applied to the control sites for approximately 30 seconds. Minocycline strip and gel was applied into the periodontal pocket at 1, 2, 3, 4 weeks each after scaling and root planing in the test sites. The clinical and microbiological measurements were made at baseline and at the follow-up visits 6, 10, 14, 20 weeks. The results of this study were as follows; 1. The sulcular bleeding index, probing pocket depth and Periocheck test was significantly reduced and the relative proportions of spirochetes and motile rods were significantly reduced and the proportion of cocci was correspondingly increased, in locally delivered minocycline strip group compared to saline irrigation group. 2. In locally delivered minocycline gel group, The effect was the same with minocycline strip group as compared with saline irrigation therapy. 3. There was no significant differences between minocycline strip group and minocycline gelgroup. In conclusion, minocycline HCl local drug delivery combined with scaling and root planing may provide added improvement of clinical and microbiological responses by inhibiting bacterial recolonization of treated sites. It is suggested that the local administration of minocycline-HCl in the periodontal pocket is effective when combined with subgingival mechanical debridement.

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Bearing of Strip Foundation on Geogrid-Reinforced Sand With Embedment Depth (기초의 근입깊이를 고려한 지오그리드 보강 사질토지반의 지지력 평가)

  • 신은철;신동훈;오영인
    • Proceedings of the Korean Geotechical Society Conference
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    • 1999.10a
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    • pp.233-240
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    • 1999
  • The laboratory tests on geogrid-reinforced sand were conducted with considering embedment effect. The relative densities of sand are 60% and 80%, respectively. The embedment depths of foundation were varied as D$\_$f/B=0, 0.5, 1.0. Based on the model test results, (u/B)$\_$cr/, BCR$\_$u/, and (b/B)$\_$cr/, were determined. The optimum depth of reinforcement was determined. The embedment depth of foundation is greatly contributed on the bearing capacity of geogrid-reinforced sand.

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Development of the rapid detection kit for Salmonella spp. using immunochromatographic assay (면역크로마토그라피 기법을 이용한 Salmonella 속균 신속 검출킷트 개발)

  • Jung, Byeong-yeal;Jung, Suk-chan
    • Korean Journal of Veterinary Research
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    • v.45 no.2
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    • pp.191-197
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    • 2005
  • An immunochromatographic (IC) strip for the rapid detection of Salmonella spp. in the enriched sample was developed. Affinity purified Salmonella polyclonal antibody was conjugated with 40 nm colloidal gold particles which were prepared by citrate method in our laboratory. The antigen-antibody-gold complex was captured by Salmonella antibody attached to test line of nitrocellulose membrane during the capillary migration of sample. Specificity of the IC strip was calculated to be 100% (12/12) and sensitivity was 97.6% (41/42) in the test with pure cultured bacteria. Salmonella was artificially inoculated into raw pork macerated with enrichment broth. And then it was 10-fold diluted from $5.2{\times}10^{8}CFU/ml$ to 5.2 CFU/ml. The IC strip could detect $5.2{\times}10^{6}CFU/ml$ before enrichment. However, the lowest limit of detection was 5.2 CFU/ml after overnight incubation. The results indicated that the IC assay was a rapid, economical and simple method with high specificity and sensitivity for the detection of Salmonella spp. without using any equipment.

A Study on the Development of Surface Defect Inspection Preprocessing Algorithm for Cold Mill Strip (냉연 표면흠 검사를 위한 전처리 알고리듬에 관한 연구)

  • Kim, Jong-Woong;Kim, Kyoung-Min;Moon, Yun-Shik;Park, Gwi-Tae;Lee, Jong-Hak;Jung, Jin-Yang
    • Proceedings of the KIEE Conference
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    • 1996.07b
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    • pp.1240-1242
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    • 1996
  • In a still mill, the effective surface defect inspection algorithm is necessary. For this purpose, this paper proposed the preprocessing algorithm for surface defect inspection of cold mill strip. This consists of live steps. They are edge detection, binarizing, noise deletion, combining of fragmented defect and selecting the largest defect. Especially, binarizing is a critical problem. Bemuse the performance of the preprocessing is largely depend on the binarized image. So, we develope the adaptive thresholding method, which is multilevel thresholding. The thresholding value is varied according to the mean graylevel value of each test image. To investigate the performance of the proposed algorithm, we classified the detected defect using neural network. The test image is 20 defect images captured at German Sick Co. This algorithm is proved to have good property in cold mill strip surface inspection.

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A Study on Failure Mechanism of Reinforced Earth Retaining Wall under Strip Load (대상하중하의 보강토옹벽의 파괴 메카니즘에 관한 연구)

  • 유남재;김영길
    • Geotechnical Engineering
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    • v.7 no.4
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    • pp.35-48
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    • 1991
  • Based on centrifuge model tests, the failure mechanism of reinforced earth retaining wall under strip load was investigated in this paper. Tests were performed by changing the materials of reinforcing strips, strip lengths, and strip arrangements. The strips were strain-gauged to measure the tensions in strips. The results were analyzed and compared with various design methosds in use to verify their feasibility. Consequently, a centrifuge model test was an effective method of investigating the behavior of reinforced earth retaining wall. The 2 : 1 stress diffusion method showed comparable results with tests in estimating the capacity of the reinforced earth wall under strip load. The superposition of tensions due to selfweight of the backfill and strip load was valid to estimate total tensions mobilized in strips. Using the elasticity theory to estimate the maximum tension mobilized in strips due to surcharge, while solutions of Boussinesq and Westergaard underestimated less tensions than the measured valises, Frohlich solution showed the comparable results with tests.

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Prediction of Three-Dimensional Strip Profile for 6-High Mill in Thin-Strip Rolling (6 단 압연기의 극박 압연공정에서 3 차원 판 형상 예측)

  • Lee, Sang-Ho;Song, Gil-Ho;Lee, Sung-Jin;Kim, Byung-Min
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.35 no.8
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    • pp.855-861
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    • 2011
  • We predict the rolled-strip profile for a 6-high mill using thin rolling theory and a numerical model. In the numerical model, we calculate the distributions of the contact pressures between the rolls and the rolling pressure between the strip and the work roll in the transverse direction using the geometric structure of the 6-high mill and the boundary conditions. We determine the distribution of the rolling pressure in the rolling direction via a thin-foil rolling model using Fleck's theory. We calculate the three-dimensional elastic deformation of the work roll using the pressures of the width and rolling directions. We then obtain the three-dimensional strip profile via the elastic deformation of the work roll during the rolling process. The profile is verified by a thin cold-rolling test and FE simulation.

Numerical Prediction of Permanent Deformation of Automotive Weather Strip (자동차용 웨더스트립의 영구변형 예측)

  • Park, Joon-Chul;Min, Byung-Kwon;Oh, Jeong-Seok;Moon, Hyung-Il;Kim, Heon-Young
    • Transactions of the Korean Society of Automotive Engineers
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    • v.18 no.4
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    • pp.121-126
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    • 2010
  • The automotive weather strip has functions of isolating of water, dust, noise and vibration from outside. To achieve good sealing performance, weather strip should be designed to have the high contact force and wide contact area. However, these design causes excessive permanent deformation of weather strip. The causes of permanent deformation is generally explained to be the chemical material detrioration and physical variation and cyclic loading, etc. This paper introduces a numerical method to predict the permanent deformation using the time dependent viscoelastic model which is represented by Prony series in ABAQUS. Uniaxial tension and creep tests were conducted to obtain the material data. And the lab. test for the permanent deformation was accelerated during shorter time, 300 hours. The permanent deformation of weather strip was successfully predicted under the different loading conditions and different section shapes using the suggested numerical process.