Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Development of the rapid detection kit for Salmonella spp. using immunochromatographic assay

면역크로마토그라피 기법을 이용한 Salmonella 속균 신속 검출킷트 개발

  • Jung, Byeong-yeal (Veterinary Pharmacology Division, National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry) ;
  • Jung, Suk-chan (Livestock Products Standard Division, National Veterinary Research and Guarantine Service, Ministry of Agriculture and Forestry)
  • 정병열 (농림부 국립수의과학검역원 동물약품과) ;
  • 정석찬 (농림부 국립수의과학검역원 축산물규격과)
  • Accepted : 2005.05.20
  • Published : 2005.06.30
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Abstract

An immunochromatographic (IC) strip for the rapid detection of Salmonella spp. in the enriched sample was developed. Affinity purified Salmonella polyclonal antibody was conjugated with 40 nm colloidal gold particles which were prepared by citrate method in our laboratory. The antigen-antibody-gold complex was captured by Salmonella antibody attached to test line of nitrocellulose membrane during the capillary migration of sample. Specificity of the IC strip was calculated to be 100% (12/12) and sensitivity was 97.6% (41/42) in the test with pure cultured bacteria. Salmonella was artificially inoculated into raw pork macerated with enrichment broth. And then it was 10-fold diluted from $5.2{\times}10^{8}CFU/ml$ to 5.2 CFU/ml. The IC strip could detect $5.2{\times}10^{6}CFU/ml$ before enrichment. However, the lowest limit of detection was 5.2 CFU/ml after overnight incubation. The results indicated that the IC assay was a rapid, economical and simple method with high specificity and sensitivity for the detection of Salmonella spp. without using any equipment.

Keywords

References

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