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Development of the rapid detection kit for Salmonella spp. using immunochromatographic assay  

Jung, Byeong-yeal (Veterinary Pharmacology Division, National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry)
Jung, Suk-chan (Livestock Products Standard Division, National Veterinary Research and Guarantine Service, Ministry of Agriculture and Forestry)
Publication Information
Korean Journal of Veterinary Research / v.45, no.2, 2005 , pp. 191-197 More about this Journal
Abstract
An immunochromatographic (IC) strip for the rapid detection of Salmonella spp. in the enriched sample was developed. Affinity purified Salmonella polyclonal antibody was conjugated with 40 nm colloidal gold particles which were prepared by citrate method in our laboratory. The antigen-antibody-gold complex was captured by Salmonella antibody attached to test line of nitrocellulose membrane during the capillary migration of sample. Specificity of the IC strip was calculated to be 100% (12/12) and sensitivity was 97.6% (41/42) in the test with pure cultured bacteria. Salmonella was artificially inoculated into raw pork macerated with enrichment broth. And then it was 10-fold diluted from $5.2{\times}10^{8}CFU/ml$ to 5.2 CFU/ml. The IC strip could detect $5.2{\times}10^{6}CFU/ml$ before enrichment. However, the lowest limit of detection was 5.2 CFU/ml after overnight incubation. The results indicated that the IC assay was a rapid, economical and simple method with high specificity and sensitivity for the detection of Salmonella spp. without using any equipment.
Keywords
Salmonella; immunochromatographic assay; colloidal gold;
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1 국립보건원. 배양확인 살모넬라 분리 현황, 1996-2002 년. 감염병발생정보. 제13권 제12호, pp. 203, 2002
2 정병열, 정석찬, 조동희, 김종염, 박용호, 신쌍재, 김성국, 김봉환. 식육중 Escherichia coli O157 검출을 위한 enzyme immunoassay 기법 개발. 대한수의학회지 1998, 38, 745-750
3 Bautista DA, Elankumaran S, Arking JA, Heckert RA. Evaluation of an immunochromatography strip assay for the detection of Salmonella sp. from poultry. J Vet Diagn Invest 2002, 14, 427-430   DOI   ScienceOn
4 Borch E, Nesbakken T, Christensen H. Hazard identification in swine slaughter with respect to foodborne bacteria. Int J Food Microbiol 1996, 30, 9-25   DOI   ScienceOn
5 DiScipio RG. Preparation of colloidal gold particles of various sizes using sodium borohydride and sodium cyanoborohydride. Anal Biochem 1996, 236, 168-170   DOI   PUBMED   ScienceOn
6 June GA, Sherrod PS, Andrews WH. Comparison of two enzyme immunoassay for recovery of Salmonella spp. from four low-moisture foods. J Food Prot 1992, 55, 601-604   DOI
7 Paek SH, Lee SH, Cho JH, Kim YS. Development of rapid one-step immunochromatographic assay. Methods 2000, 22, 53-60   DOI   ScienceOn
8 Chiao DJ, Shyu RH, Hu CS, Chiang HY, Tang SS. Colloidal gold-based immunochromatographic assay for detection of botulinum neurotoxin type B. J Chromatogr 2004, 809, 37-41   DOI   ScienceOn
9 Kenna JG, Major GN, Williams RS. Methods for reducing non-specific antibody binding in enzymelinked immunosorbent assays. J Immunol Methods 1985, 85, 409-419   DOI   ScienceOn
10 정윤희, 오승건. 생닭: 50개소중 34개소의 생닭에서 식중독균 검출. pp. 52-55, 소비자시대. 한국소비자보호원, 11월호, 1998
11 김규태, 정병열, 김봉환. 경북지역 가축에서 분리한 Salmonella 속균의 혈청형 분포 및 약제 감수성. 한국수의공중보건학회지 2003, 27, 47-52
12 Grimont PAD, Grimont F, Bouvet P. Taxonomy of the genus Salmonella. In: Wray C, Wray A(eds.). Salmonella in Domestic Animals. pp. 1-17, CABI Publishing, 2000
13 Roth J. The protein A-gold (pAg) technique: a qualitative and quantitative approach for antigen localization on thin sections. In: Bullock GR, Petrusz P(eds.). Techniques in Immunocytochemistry. Vol. 1, pp. 107-133, Academic Press, London, 1982
14 de Boer E, Beumer RR. Methodology for detection and typing of foodborne microorganisms. Int J Food Microbiol 1999, 50, 119-130   DOI   PUBMED   ScienceOn
15 Ruiz J, Nunez ML, Diaz J, Sempere MA, Gomez J, Usera MA. Note: comparison of media for the isolation of lactose-positive Salmonella. J Appl Bacteriol 1996, 81, 571-574
16 Roth J. The colloidal gold marker system for light and electron microscopic cytochemistry. In: Bullock GR, Petrusz P(eds.). Techniques in Immunocytochemistry. Vol. 2, pp. 216-284, Academic Press, London, 1983
17 Slot JW, Geuze HJ. Gold markers for single and double immunolabelling of ultrathin cryosections. In: Polak JM, Varndell IM(eds.). Immunolabelling for electron microscopy. pp. 129-142, Elsevier, Amsterdam, 1984
18 Nakamura T, Otaki Y, Lin Z, Kato A. Rapid and quantitative assay system for measuring anti-infectious bursal disease virus antibody using monoclonal antibody bound to polystyrene latex microspheres. Avian Dis 1993, 37, 786-792   DOI   ScienceOn
19 Mallinson ET, Miller RG, de Rezende CE, Ferris KE, deGraft-Hanson J, Joseph SW. Improved plating media for the detection of Salmonella species with typical and atypical hydrogen sulfide production. J Vet Diagn Invest 2000, 12, 83-87   DOI   ScienceOn
20 Wilson G. Rapid and economical method for biochemical screening of stool isolates for Salmonella and Shigella species. J Clin Microbiol 2004, 42, 4821-4823   DOI   PUBMED   ScienceOn
21 Geoghegan WD, Ambegaonkar S, Calvanico NJ. Passive gold agglutination. An alternative to passive hemagglutination. J Immunol Methods 1980, 34, 11-21   DOI   ScienceOn