• Journal of Microbiology and Biotechnology
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• 제26권7호
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• pp.1267-1277
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• 2016

Currently, enzymatic synthesis of lactulose, a synthetic prebiotic disaccharide, is commonly performed with glycosyl hydrolases. In this work, a new type of lactulose-producing biocatalyst was developed by displaying β-galactosidase from Bacillus stearothermophilus IAM11001 (Bs-β-Gal) on the surface of Bacillus subtilis 168 spores. Localization of β-Gal on the spore surface as a fusion to CotX was verified by western blot analysis, immunofluorescence microscopy, and flow cytometry. The optimum pH and temperature for the resulting spore-displayed β-Gal was 6.0 and 75℃, respectively. Under optimal conditions, it showed maximum activity of 0.42 U/mg spores (dry weight). Moreover, the spore-displayed CotX-β-Gal was employed as a whole cell biocatalyst to produce lactulose, yielding 8.8 g/l from 200 g/l lactose and 100 g/l fructose. Reusability tests showed that the spore-displayed CotX-β-Gal retained around 30.3% of its initial activity after eight successive conversion cycles. These results suggest that the CotX-mediated spore-displayed β-Gal may provide a promising strategy for lactulose production.

• Journal of Microbiology and Biotechnology
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• 제26권7호
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• pp.1234-1241
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• 2016

Methanol is a versatile compound that can be biologically synthesized from methane (CH₄) by methanotrophs using a low energy-consuming and environment-friendly process. Methylocella tundrae is a type II methanotroph that can utilize CH₄ as a carbon and energy source. Methanol is produced in the first step of the metabolic pathway of methanotrophs and is further oxidized into formaldehyde. Several parameters must be optimized to achieve high methanol production. In this study, we optimized the production conditions and process parameters for methanol production. The optimum incubation time, substrate, pH, agitation rate, temperature, phosphate buffer and sodium formate concentration, and cell concentration were determined to be 24 h, 50% CH₄, pH 7, 150 rpm, 30℃, 100 mM and 50 mM, and 18 mg/ml, respectively. The optimization of these parameters significantly improved methanol production from 0.66 to 5.18 mM. The use of alginate-encapsulated cells resulted in enhanced methanol production stability and reusability of cells after five cycles of reuse under batch culture conditions.

• Journal of Microbiology and Biotechnology
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• 제20권2호
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• pp.325-331
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• 2010

Rhodococcus erythropolis amidase was expressed in Escherichia coli cells. The crude amidase in the cell-free extract was immobilized using the cross-linked enzyme aggregate (CLEA) method. The crude amidase was mixed with bovine serum albumin and then precipitated with ammonium sulfate. The resultant precipitant was subsequently cross-linked with glutaraldehyde. Scanning electron microscopy revealed that this co-CLEA had a ball-like shape with a diameter of approximately $1\;{\mu}m$. This co-CLEA evidenced hydrolytic activity toward a variety of amide substrates. The amidase co-CLEA evidenced an optimum temperature of $60^{\circ}C$ and an optimum pH of 8.0, results that were similar to those of the soluble amidase. The reaction stability of the co-CLEA was increased. That is, it was stable up to $50^{\circ}C$ and in a pH range of 5.0-12.0. Additionally, the co-CLEA could be recovered by centrifugation, and retained 96% activity after 3 repeated cycles. This amidase co-CLEA may prove useful as a substitute for soluble amidase as a biocatalyst in the pharmaceutical and chemical industries.

• 한국초전도학회:학술대회논문집
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• 한국초전도학회 1999년도 High Temperature Superconductivity Vol.IX
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• pp.7-11
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• 1999

The recent progress in new cooling techniques of the high Tc superconductor(HTS) systems is reported and discussed with some practical examples. At the beginning stage of the HTS development in research laboratories, liquid nitrogen(LN$_2$) is the standard medium for an effective cooling. The success of HTS in many different application areas, however, has required a variety of need in the cooling temperature and the cooling capacity with specific design restrictions. While the utilization of alternative liquid cryogens such as liquid neon (LNe) or liquid hydrogen (LH$_2$) has been tired in some of them, even solid cryogens such as solid nitrogen (SN$_2$) or solid hydrogen (SH$_2$) may be another option in special applications. The gaseous helium cooled by a cryogenic refrigerator has also been a good candidate in many cases. One of the best cooling methods for the HTS is the direct conduction-cooling by a closed-cycle refrigerator with no cryogen at all. The refrigeration may be based on Joul-Thomson, Brayton, Stirling, Gifford-McMahon, or pulse tube cycles. The pros and cons of the newly proposed cooling methods are described and some significant design issues are presented.

• 한국항공우주학회지
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• 제39권7호
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• pp.612-617
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• 2011

동합금 Elbrodur-NIB 재질에 대해 평균응력을 포함한 응력제어시험을 수행하였으며, 그 결과 라체팅 거동에 응력진폭, 평균응력, 응력속도가 어떠한 영향을 주는지에 대해 시험을 통해 알 수 있었다. 라체팅 변형률은 같은 평균응력에서 응력진폭이 증가할수록, 또한 같은 응력진폭에서 평균응력이 증가함에 따라 같이 증가하는 것을 볼 수 있었다. 그러나, 응력속도가 증가함에 따라 라체팅 변형률은 감소하는 것을 알 수 있었다. $10^2-10^5$ 영역에서 3가지 평균응력을 포함한 피로수명예측모델을 검토하였으며, 그 결과 Smith-Watson-Topper가 제안한 모델과 Walker가 제안한 모델이 매우 신뢰성 있는 결과를 보여주었다.

• 한국마이크로전자및패키징학회:학술대회논문집
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• 한국마이크로전자및패키징학회 2007년도 SMT/PCB 기술세미나
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• pp.37-47
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• 2007

Factor : Structure Metal pad & SMO size Board level TC test : - Large SMO size better Board level Drop test : - Large SMO size better Factor : Structure Substrate thickness Board level TC test : - Thick substrate better Board level Drop test : - Substrate thickness is not a significant factor for drop test Factor : Material Solder alloy Board level TC test : - Not so big differences over Pb-free solder and NiAu, OSP finish Board level Drop test : - Ni/Au+SAC105, CuOSP+LF35 are better Factor : Material Pad finish Board level TC test : - NiAu/NiAu is best Board livel Drop test : - CuOSP is best Factor : Material Underfill Board level TC test - Several underfills (reworkable) are passed TCG x500 cycles Board level Drop test : - Underfill lots have better performance than non-underfill lots Factor : Process Multiple reflow Board level TC test : - Multiple reflow is not a significant actor for TC test Board level Drop test : N/A Factor : Process Peak temp Board level TC test : - Higher peak temperature is worse than STD Board level Drop test : N/A Factor : Process Stack method Board level TC test : - No big difference between pre-stack and SMT stack Board level Drop test : - Flux dipping is better than paste dipping but failure rate is more faster

• 한국수소및신에너지학회논문집
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• 제18권4호
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• pp.382-390
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• 2007

To check the feasibility of SMART(Steam Methane Advanced Reforming Technology) system, an experimental investigation was performed. A fluidized bed reactor of diameter 0.052m was operated cyclically up to 10th cycle, alternating between reforming and regeneration conditions. FCR-4 catalyst was used as the reforming catalyst and calcined limestone(domestic, from Danyang) was used as the $CO_2$ absorbent. Hydrogen concentration of 98.2% on a dry basis was reached at $650^{\circ}C$ for the first cycle. This value is much higher than $H_2$ concentration of 73.6% in the reformer of conventional SMR (steam methane reforming) condition. The hydrogen concentration decreased because the $CO_2$ capture capacity decreased as the number of cycles increased. However, the average hydrogen concentration at 10th cycle was 82.5% and this value is also higher than that of SMR. Based on these results, we could conclude that the SMART system can replace SMR system to generate pure hydrogen without HTS (high tempeature shift), LTS (low temperature shift) and $CO_2$ separation process.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2008년도 추계 학술발표회 제20권2호
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• pp.25-28
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• 2008

일반적으로 철근콘크리트 건축물은 외부의 기후에 노출되어 있어 겨울에서 이른 봄까지 동결과 융해의 반복적인 작용에 영향을 받는다. 이러한 동결융해 작용은 콘크리트의 균열을 발생시키거나 콘크리트 표면의 박리를 일으켜 내구성 저하의 원인이 된다$^{1),2)}$. 본 연구에서는 철근콘크리트보의 동결융해 경험에 따른 반복하중 하에서의 이력 특성의 평가를 위해 동결융해 사이클을 변수로 하여 철근콘크리트보의 반복하중 가력시 거동을 비교하고자 6개의 시험체를 제작, 실험하였다. 실험결과를 통해 동결 융해에 노출되어있는 철근콘크리트보의 반복하중하의 이력특성을 평가하는데 기초적인 자료를 제시하고자 하였다.

• Carbon letters
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• 제25권
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• pp.103-112
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• 2018

Hierarchically porous, chemically activated carbon materials are readily derived from biomass using hydrothermal carbonization (HTC) and chemical activation processes. In this study, empty fruit bunches (EFB) were chosen as the carbon source due to their sustainability, high lignin-content, abundance, and low cost. The lignin content in the EFB was condensed and carbonized into a bulk non-porous solid via the HTC process, and then transformed into a hierarchical porous structure consisting of macro- and micropores by chemical activation. As confirmed by various characterization results, the optimum activation temperature for supercapacitor applications was determined to be $700^{\circ}C$. The enhanced capacitive performance is attributed to the textural property of the extremely high specific surface area of $2861.4m^2\;g^{-1}$. The prepared material exhibited hierarchical porosity and surface features with oxygen functionalities, such as carboxyl and hydroxyl groups, suitable for pseudocapacitance. Finally, the as-optimized nanoporous carbons exhibited remarkable capacitive performance, with a specific capacitance of $402.3F\;g^{-1}$ at $0.5A\;g^{-1}$, a good rate capability of 79.8% at current densities from $0.5A\;g^{-1}$ to $10A\;g^{-1}$, and excellent life cycle behavior of 10,000 cycles with 96.5% capacitance retention at $20A\;g^{-1}$.

• Journal of information and communication convergence engineering
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• 제6권1호
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• pp.38-42
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• 2008

Highly sensitive and mechanically stable gas sensors have been fabricated using the microfabrication and micromachining techniques. The sensing materials used to detect the offensive $CH_3SH$ and $(CH_3)_3N$ gases are 1 wt% Pd-doped $SnO_2$ and 6 wt% $Al_2O_3$-doped ZnO, respectively. The optimum operating temperatures of the devices are $250^{\circ}C$ and $350^{\circ}C$ for $CH_3SH$ and $(CH_3)_3N$, respectively and the corresponding heater power is, respectively, about 55mW and 85mW. Excellent thermal insulation is achieved by the use of a double-layer membrane: i.e. $0.2{\mu}m$-thick silicon nitride and $1.4{\mu}m$-thick phosphosilicate glass. The sensors are mechanically stable enough to endure the heat cycles between room temperature and $350^{\circ}C$, at least for 30 days.