• Title/Summary/Keyword: tcdd

Search Result 224, Processing Time 0.033 seconds

Protective Effects of Bear Bile against Hepatotoxicity Induced by 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) in Mice (마우스에서 2,3,7,8-Tetrachlorodibenzo-p-dioxin(TCDD)에 의해 유발된 간독성에 대한 웅담의 방어효과)

  • Zhang, Hu-Song;Nam, Sang-Yoon;Kang, Jong-Koo
    • Korean Journal of Pharmacognosy
    • /
    • v.32 no.2 s.125
    • /
    • pp.121-127
    • /
    • 2001
  • The effect of bear bile on 2,3,7,8-tetrachlorodibenzo- p-dioxin (TCDD)-induced hepatotoxicity was investigated in 6-week-old C57BL/6 male mice. Bear bile (100 mg/kg or 500 mg/kg) was administered orally daily for 4 weeks, respectively. From the second week, $10\;{\mu}g/kg$ of TCDD was administered to the bear bile-treated animals orally once a week for 3 weeks (a total of $30\;{\mu}g/kg$). There were no specific clinical findings and significant body weight changes in all groups. Although the livers in TCDD-treated mice appeared a severe hypertrophy and many necrotic foci, and changed to yellow-brown color in gross findings, these lesions were remarkably reduced by bear bile administration. The elevated serum activities of alanine transaminase, aspartate transaminase, alkaline phosphatase, and lactate dehydrogenase due to TCDD were significantly decreased by bear bile treatment (P<0.05). The lipid peroxidation induced by TCDD was significantly prevented by bear bile administration (P<0.05). In histological examinations, there were a moderate necrosis of hepatic cells around central veins, severe cytoplasmic vacuolizations, inflammatory cell infiltrations, and remarkable fatty changes in the liver of TCDD-treated animals. However, the lesions were dose-dependently inhibited by the bear bile treatments. These findings indicate that bear bile may have a protective effect against TCDD-induced hepatotoxicity in mice.

  • PDF

Early Life Stage Toxicity for 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in Crucian Carp (Carassius auratus) (붕어 수정란을 이용한 다이옥신의 초기발생단계 독성평가)

  • Park, Yong-Joo;Kim, Ha-Ryong;Lee, Min-Jee;Lee, Wan-Ok;Lee, Jung-Sick;Chung, Kyu-Hyuck;Oh, Seung-Min
    • Environmental Analysis Health and Toxicology
    • /
    • v.25 no.3
    • /
    • pp.241-251
    • /
    • 2010
  • Crucian carp (Carassius auratus) has been used as the sentinel species for POPs (Persistent Organic Pollutants) monitoring in aquatic environment. However, there is little information for dioxin toxicity and especially, early life stage toxicity in crucian carp have been never carried out. In this study, we investigated several toxic effects for 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) in fertilized egg obtained by natural fertilization from crucian carp. The embryos at 3 h post-fertilization (hpf) were treated with 0.039, 0.156, 0.625, and 2.5 (${\mu}g/L$) TCDD by waterborne exposure for 60 minutes and changed with fresh water 2 times per day. Fertilized eggs started hatching at 51 hpf and TCDD exposed embryo showed decrease of hatching rate in a dose-dependent manner at 75 hpf. Pericardial edema was continuously observed in larvae exposed to TCDD from hatching start time (51 hpf), followed by the onset of mortality. In addition, AhR-related gene, CYP1A was clearly increased by TCDD in a dose dependent manner. These results indicated that fertilized eggs obtained from crucian carp have the TCDD related gene regulation and a distinct TCDD developmental toxicity syndrome by TCDD exposure. Therefore, we suggested that early life stage test in crucian carp could be used as test methods on dioxins toxicity.

Effects of Green Tea Powder on the Disorders of Lipid Metabolism and Hepatic Functions in Rats treated by 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) (녹차가 다이옥신계 TCDD(2,3,7,8-tetrachlorodibenzo-p-dioxin)에 노출된 흰쥐의 지질대사 및 간 독성물질대사에 미치는 영향)

  • Lee, Joon-Ho;Kim, Hyun-Sook;Hwang, Seok-Youn
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.35 no.9
    • /
    • pp.1185-1193
    • /
    • 2006
  • This study investigated the effects of green tea on the disorders of lipid metabolism, oxidative system and hepatic functions induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), using adult male rats (SD) for 3 weeks. These 36 animals were divided into four groups. TCDD ($50{\mu}g/kg$ BW) was intraperitoneally injected at the beginning of experiment. Green tea powder was added 1% or 3% levels in basal diets respectively. Relative weights of thymus were decreased about one-third of control group, but those of liver, brain and testis were significantly increased in rats treated TCDD. Neutrophill% and lymphocyte% by TCDD treatment was improved by green tea diets. In liver functional enzyme, elevation of glutamic oxaloacetic transaminase (GOT) and alkaline phosphatase (ALP) activities due to TCDD treatment was lowered by green tea diets. The concentrations of serum and liver lipids were significantly increased by TCDD treatment, however, those of serum and liver triglyceride tended to decrease by green tea diets. Fecal lipid excretion was increased in rats fed green tea diets. Especially, fecal total cholesterol level was significantly elevated by 3% green tea diets. The activities of superoxide dismutase (SOD) were increased in rats fed 3% green tea diets. Increment of benzphetamine N-demethylase (BPND) activity by TCDD treatment was declined by 1% green tea diets. These results indicate that green tea can exert improving effects on liver lipid accumulation and unfavorable hepatic functions, and elevate antioxidation.

Ethanol Extract of Antler Velvet Attenuates Testicular Toxicity Induced by 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD ) in Rats (랫드에 있어 녹용 알콜 추출물의 TCDD-유발 고환 독성 방어 효과)

  • Choi, Kyung-Yun;Hwang, Seock-Yeon;Kim, Si-Kwan
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.34 no.8
    • /
    • pp.1169-1174
    • /
    • 2005
  • This study was carried out to investigate the biological effect of deer's antler velvet on the testicular toxicity of rats exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD ). Thirty male rats were divided into three equal groups. The control group received vehicle (DMSO/acetone/soybean oil mixture) and saline; single dose of 50 $/mu$g/kg body weight TCDD was injected intraperitoneally into the single TCDD-treated and test group. Test group received ethanol extract of antler velvet (EAV) at daily dose of 20 mg/kg body weight for 5 weeks from one week before TCDD exposure. Decrease in body weight increment was less remarkable in test group compared with that of TCDD-treated group. TCDD-induced decrease in testicular weight, microtubular diameter and Johnson's score, and lesion were significantly alleviated by the treatment of EAV. This result led us to the conclusion that antler velvet can attenuate TCDD-induced testicular toxicity in rats.

The Effects of Gobonyangjeonbang Administration on Reproductive Toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induced Rats. (고본양정방 투여가 TCDD 유도 랫드의 생식독성에 미치는 영향)

  • OH, Ji Hye;Yang, Dong Hyun;Park, Un kyu;Cho, Chung Sik;Hwang, Seock Yeon
    • Journal of the Korea Academia-Industrial cooperation Society
    • /
    • v.22 no.4
    • /
    • pp.504-512
    • /
    • 2021
  • Sperm formation disorders and sperm quality degradation comprises the largest proportion of male infertility caused by TCDD. To solve this problem, this study examined the effects of Gobonyangjeonbang oriental medicine prescription on the endocrine function and reproductive toxicity-related indicators in rat-induced TCDD-induced reproductive. Male SD rats were divided into five groups of seven animals and tested. The normal control group was administered the vehicle and saline, the TCDD alone group was administered intraperitoneally with TCDD (2 ㎍/kg, weeks) and physiological saline, and the test group was administered orally by dividing GYB (75, 150, and 300 mg/kg) into three concentrations for six weeks. Weight loss was observed in all groups administered TCDD. Regarding the hormonal changes, a significant decrease in free testosterone was observed in the GYB 300 mg/kg group (p<0.01). In addition, some of the germ cell destruction, seminiferous tubular atrophy, and decrease in sperm count was improved in a concentration-dependent manner in the testicular tissue of the GYB-treated group. In addition, Johnsen's score and serotoli cell index (SCI) were improved in a concentration-dependent manner (p<0.05). Overall, GYB can be used in drug therapy rather than medical procedures to solve male infertility in the future.

CYP4501Al gene expression by TCDD in Hepa I cells.

  • Cha Y. Baek;Kim, Yeo W.;Yhun. Y. Sheen
    • Proceedings of the Korean Society of Applied Pharmacology
    • /
    • 1998.11a
    • /
    • pp.139-139
    • /
    • 1998
  • Effects of TCDD and flavonoids on ethoxyresorufin deethylase in Hepa I cells and MCF-7 human breast cancer cells were examined. TCDD treatment have resulted in the stimulation of ethoxyresorufin deethylase activity based on fluorometry in Hepa I in dose and time dependent manner. 0.1 nM TCDD showed maximal stimulation of ethoxyresorufin deethylase activity and 24 hour treatment also showed maximal stimulation of ethoxyresorufin deethylase activity. In MCF-7 human breast cancer cells, untreated cells showed high basal level of ethoxyresorufin deethylase activity. TCDD treatment to MCF-7 cells resulted minor stimulation of ethoxyresorufin deethylase activity compared to that in Hepa I cells. Various chemicals were tested for ethoxyresorufin deethylase activity in both cell lines. Flavonoids, such as quercetin showed an inhibition of ethoxyresorufin deethylase activity that is stimulated with TCDD or 3-Methylcholanthrene. Estrogen and estrogen metabolites such as 16a-estriol also affects the ethoxyresorufin deethylase activity in MCF-7 cells.

  • PDF

P18 Nitric Oxide and Hypoxia Affect TCDD Induced EROD Activity

  • Kim, Yeo W.;Cha Y. Baek;Hong K. Min;Yhun. Y. Sheen
    • Proceedings of the Korean Society of Applied Pharmacology
    • /
    • 1998.11a
    • /
    • pp.138-138
    • /
    • 1998
  • Effects of nitric oxide and hypoxia on ethoxyresorufin deethylase in Hepa I cells and MCF-7 human breast cancer cells were examined. TCDD treatment have resulted in the stimulation of ethoxyresorufin deethylase activity based on fluorometry in Hepa I in dose and time dependent manner. 0.1 nM TCDD showed maximal stimulation of ethoxyresorufin deethylase activity and 24 hour treatment also showed maximal stimulation of ethoxyresorufin deethylase activity. In MCF-7 human breast cancer cells, untreated cells showed high basal level of ethoxyresorufin deethylase activity. TCDD treatment to MCF-7 cells resulted minor stimulation of ethoxyresorufin deethylase activity compared to that in Hepa I cells. Nitric oxide and hypoxia inhibit TCDD effects on ethoxyresorufin deethylase activity in both cell lines. And also flavonoids, such as quercetin showed an inhibition of ethoxyresorufin deethylase activity that is stimulated with TCDD or 3-Methylcholanthrene. Estrogen and estrogen metabolite such as 16 a-estriol and 2-hydroxyestradiol also affects the ethoxyresorufin deethylase activity in MCF-7 cells.

  • PDF

Histopathological Study on the Protective Effect of Korean Red Ginseng on TCDD-induced Acute Toxicity in Male Guinea Pig (TCDD 투여로 급성독성을 유도한 웅성 기니픽에 있어 홍삼의 방어 효과에 대한 병리조직학적 연구)

  • Hwang Seock-Yeon;Jeong Hwa-Sook;Wee Jae-Joon;Sung Rohyun;Kim Si-Kwan
    • Journal of Ginseng Research
    • /
    • v.23 no.4
    • /
    • pp.222-229
    • /
    • 1999
  • Histopathological study has been carried out to elucidate the protective effect of Korean red ginseng water extract (KRG-WE) on 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced acute toxicity in male guinea pigs. Forty male guinea pigs ($200{\pm}20g$) were divided into 4 groups: normal controls (group 1) received vehicle and saline; group 2 (single TCDD-treated) received TCDD (5 ${\mu}g/kg$, single dose) and saline; group 3 received KRG-WE (200 mg/kg, i.p.) for 2 weeks starting 1 week before TCDD-exposure; group 4 received same dose of KRG-WE for 7 days from the day of TCDD-exposure. Weights of liver, testis, kidney, spleen and lung of the TCDD-exposed guinea pigs were significantly decreased. Thymus was severely shrunken, thereby could not be distinguished from adipose tissue in group 2 animals. Focal interstitial inflammation and fibrosis were observed from the lung parenchyma of group 2 animals. Furthermore, moderate swelling of hepatocyte, diffused aggregates of hemosiderin-laden macrophages from the Prussian blue stained spleen, marked decrease in spermatogenesis, and pyknotic and degenerative changes in the renal tubules were observed from intestinal organs of group 2 animals. On the other hand, histopathological damage was moderately to markedly alleviated in groups 3 and 4, but pretreatment of KRG-WE was more effective than the simultaneous treatment. In particular, TCDD-induced testicular atrophy was significantly attenuated by KRG-WE (p<0.01). From these results, it could be suggested that Korean red ginseng might be a useful herb that prevented TCDD-induced toxicity on liver, testis, kidney and spleen.

  • PDF

Serum 2,3,7,8-Tetrachlorodibenzo-p-dioxin Levels and Their Association With Age, Body Mass Index, Smoking, Military Record-based Variables, and Estimated Exposure to Agent Orange in Korean Vietnam Veterans

  • Yi, Sang-Wook;Ohrr, Heechoul;Won, Jong-Uk;Song, Jae-Seok;Hong, Jae-Seok
    • Journal of Preventive Medicine and Public Health
    • /
    • v.46 no.5
    • /
    • pp.226-236
    • /
    • 2013
  • Objectives: The aim of this study was to examine the levels of serum 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and evaluate their association with age, body mass index, smoking, military record-based variables, and estimated exposure to Agent Orange in Korean Vietnam veterans. Methods: Serum levels of TCDD were analyzed in 102 Vietnam veterans. Information on age, body mass index, and smoking status were obtained from a self-reported questionnaire. The perceived exposure was assessed by a 6-item questionnaire. Two proximitybased exposures were constructed by division/brigade level and battalion/company level unit information using the Stellman exposure opportunity index model. Results: The mean and median of serum TCDD levels was 1.2 parts per trillion (ppt) and 0.9 ppt, respectively. Only 2 Vietnam veterans had elevated levels of TCDD (>10 ppt). The levels of TCDD did not tend to increase with the likelihood of exposure to Agent Orange, as estimated from either proximity-based exposure or perceived self-reported exposure. The serum TCDD levels were not significantly different according to military unit, year of first deployment, duration of deployment, military rank, age, body mass index, and smoking status. Conclusions: The average serum TCDD levels in the Korean Vietnam veterans were lower than those reported for other occupationally or environmentally exposed groups and US Vietnam veterans, and their use as an objective marker of Agent Orange exposure may have some limitations. The unit of deployment, duration of deployment, year of first deployment, military rank, perceived self-reported exposure, and proximity-based exposure to Agent Orange were not associated with TCDD levels in Korean Vietnam veterans. Age, body mass index and smoking also were not associated with TCDD levels.

Effect of Chitosan on the Lipid Metabolism in Treated 2,3,7,8-Tetrachlorodibenzo-p-dioxin in Rats (다이옥신 처리에 대한 키토산이 지질대사에 미치는 효과)

  • Hwang Seock Yeon;Yang Jin Bae;Chang Cheoul Soo;Kim Tae Up;Lee Hyung Chul
    • Journal of Physiology & Pathology in Korean Medicine
    • /
    • v.16 no.4
    • /
    • pp.782-787
    • /
    • 2002
  • This study was carried out to investigate the protective effect of chitosan on lipid peroxidation and key lipid parameters in Sprague-Dawley rat (SO rat) accutely exposured to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Male SO rats received single intraperitoneal (ip) injection of TCDD (40 j.lg/kg), and were given diet containing 3 or 5% chitosan for 4 weeks from 1 week before TCDD treatment. The gain in body weight was less in group treated with TCDD than in CON group, while that of Ch/H+ TCDD group (5% chitosan diet) increased. The decrease in liver and testis weight caused by TCDD was prevented by high dietary intake of chitosan (5% chitosan). Serum (total cholesterol, triglyceride, HDL-C, and LDL-C) and liver lipid parameters (total lipid, total cholesterol, and triglyceride) were significantly elevated in TCDD-induced rats, but these parameters excluding HDL-C were significantly reduced in high dietary intake of chitosan (5% chitosan). These findings suggest that chitosan is believed to be a possible protective effect against 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in rat.