Effect of Chitosan on the Lipid Metabolism in Treated 2,3,7,8-Tetrachlorodibenzo-p-dioxin in Rats

다이옥신 처리에 대한 키토산이 지질대사에 미치는 효과

  • Hwang Seock Yeon (Department of Clinical Pathology, Chungbuk National University Hospital) ;
  • Yang Jin Bae (College of Oriental Medicine, Taejon University) ;
  • Chang Cheoul Soo (Department of Clinical Pathology, Kimcheon Health Siences College) ;
  • Kim Tae Up (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Lee Hyung Chul (Department of Pathology, College of Oriental Medicine, Wonkwang University)
  • 황석연 (충북대학교병원 임상병리과) ;
  • 양진배 (대전대학교 한의과대학) ;
  • 장철수 (김천대학 임상병리과) ;
  • 김태업 (원광대학교 한의과대학 병리학교실) ;
  • 이형철 (원광대학교 한의과대학 병리학교실)
  • Published : 2002.08.01

Abstract

This study was carried out to investigate the protective effect of chitosan on lipid peroxidation and key lipid parameters in Sprague-Dawley rat (SO rat) accutely exposured to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Male SO rats received single intraperitoneal (ip) injection of TCDD (40 j.lg/kg), and were given diet containing 3 or 5% chitosan for 4 weeks from 1 week before TCDD treatment. The gain in body weight was less in group treated with TCDD than in CON group, while that of Ch/H+ TCDD group (5% chitosan diet) increased. The decrease in liver and testis weight caused by TCDD was prevented by high dietary intake of chitosan (5% chitosan). Serum (total cholesterol, triglyceride, HDL-C, and LDL-C) and liver lipid parameters (total lipid, total cholesterol, and triglyceride) were significantly elevated in TCDD-induced rats, but these parameters excluding HDL-C were significantly reduced in high dietary intake of chitosan (5% chitosan). These findings suggest that chitosan is believed to be a possible protective effect against 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in rat.

Keywords

References

  1. Am. J. Clin. Nutr. v.61 Mechanism of action of dioxin-type chemicals, pesticides, and other xenobiotics affecting nutritional indexes. Matsumura, F.
  2. Environ. Health Perspect v.5 Toxicology of chlorinated dibenzo-p-dioxin Schwetz, B.A;Sparchu, G.L.;Row, V.K.;Gehring, P.J.;Emerson, J.L.
  3. Ann. Rev. Pharmacol. Toxicol. v.22 2,3,7,8-tetrachlorodibenzo-p- dioxin and related halogenated aromatic hydrocarbons: examination of the mechanism of toxicity. Poland, A.;Knuston J. C.
  4. Toxicol. Appl. Pharmacol. v.35 ,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD): results of a 13-week oral toxicity study in rats. Kociba, R.J.;Keeler, P.A.;Park, C.N.;Gehring, P.J.
  5. Toxicol. Appl. Pharmacol. v.145 In utero and lactational exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin: effects on development of the male and female reproductive system of the mouse. Theobald, H.M.;Peterson, R.E.
  6. Ann. Neurol. v.32 The oxidant stress hypothesis in Parkinson's disease: evidence supporting it. Fahn, S.;Cohen, G.
  7. Lancet v.344 Free radicals, antioxidants and human disease: curiosity, cause, or consequence? Halliwell, B.
  8. Environ. Health Perspect v.5 General biological effects of TCDD in laboratory animals. Harris, M.W.;Moore, J.A.;Vos, J.G.;Gupta, B.N.
  9. Toxicol. Appl. Pharmacol. v.59 Characterization of the hyperlipidemia in guinea pigs induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin. Swift, L.L.;Gasiewicz, T.A.;Dunn, D.;Soule, P.D.; Neal, R.A.
  10. Toxicol. Appl. Pharmacol. v.85 Hypercholesterolemia and the regulation of Ladrenal steroidogenesis in 2,3,7,8-tetrachlorodibenzo-p-dioxin- treated rats. Dibartolomeis, M.J.;Moore, R.W.;Peterson, R.E.;Jefcoate, C.R.
  11. Biochem. Biophys. Res. Commum. v.253 Dioxin causes a sustained oxidative stress response in the mouse. Shertzer, H.G.;Nebert, D.W.;Puga, A.;Ary, M.;Sonntag, D.;Dixon, K.;Robinson, L.J.;Cianciolo, E.;Dalton, T.P.
  12. J Biol Chem v.267 2,3,7,8- tetrachlorodibenzo-p-dioxin causes reduction of glucose transporting activities in the plasma membrane of adipose tissue and pancreas from the guinea pig. Enan, E.;Liu, P.C.C.;Matsumura, F.
  13. Am. J. Clin. Nutr. v.38 Comparative effects of chitosan and cholestyramine on lymphatic absorption of lipids in the rat. Vahouny, G.V.;Satchithanandam, S.;Cassidy, M.M.;Lightfoot, F.B.;Furda, I.
  14. Kor. J. Nutr. v.31 Effects of chitosan of different molecular weights on lipid metabolism in rats. Lee, J.M.;Son, B.K.
  15. Br. J. Nutr. v.78 Broiler chicken body weights, feed intakes, plasma lipid and small- intestinal bile acid concentrations in response to feeding of chitosan and pectin. Razdan, A.;Pettersson, D.;Pettersson, J.
  16. Am J Clin Nutr v.33 A novel use of chitosanas a hypocholesteromic agent in rats. Sugano, M.;Fujikawa, T.;Hiratsuji, Y.;Nakashima, K.;Fukuda, N.;Hasegawa, Y.
  17. Crit. Rev. Toxicol. v.24 Polychlorinated biphenyls (PCBs): environmental impact, biochemical and toxic responses, and implications for risk assessment. Safe, S.H.
  18. Food Chem. Toxicol. v.40 Risk assessment of dioxin contamination in human food. Parefall, W.
  19. Chemosphere v.15 Pharmacokinetics of 2,3,7,8- TCDD in man. Poiger, A.;Schlatter, C.
  20. 한국식품영양과학회지 v.28 2,3,7,8- Tetrachlorodibenzo-p-dioxin 투여로 급성독성을 유도한 웅성 기니픽에 있어 임상화학지수에 미치는 홍삼의 효과. 황석연;김시관;김신희;곽이성;정영진
  21. Toxicol. v.151 Bone tissue composition, dimensions and strength in female rats given an increased dietary level of vitamin A or exposed to 3,3',4,4',5- pentachlorobiphenyl (PCB 126) alone or in combination with vitamin C. Lind, P.M.;Larsson, S.;Johansson, Melhus, H.;Wikstrom, M.;Lindhe, O.;Orberg.
  22. J. Biol. Chem. v.226 A simple method for the isolation and purification of total lipids from animal tissue. Folch, J.;Less, M.; Stanely, G.H.S.
  23. Clin. Chem. v.21 A manual colorimetric assay of triglyceride in serum. Biggs, H.G.;Erikson, M.J.;Wells, R.M.
  24. Anal. Botchier. v.29 Study of a new cholesterol reagent. Zlatkis, A.; Zak, B.
  25. Methods Enzymol. v.71 3-Hydroxy-3-ethylglutaryl CoA reductase from rat liver. Kleinsek, D.A.;Dugan, R.E.;Baker, T.A.;Porter, J.W.
  26. Methods Enzymol. v.52 Microsomal lipid peroxidation. Buege, J.A.;Aust, S.D.
  27. Brazil. Environ. Res. (section A) v.83 PCDDs, PCDFs, PCBs, and other organochlorine compounds in human milk from Rio de Janeiro Paumgartten, F.J.R.;Cruz, C.M.;Chahoud, .I;Palavinskas, R.;Mathar, W.
  28. Ann. Rev. Pharmacol. Toxicol. v.22 2,3,7,8-tetrachlorodibenzo- p-dioxin and related halogenated aromatic hydrocarbons: examination of the mechanism of toxicity. Poland, A.;Knuston J. C.
  29. Toxicol. Appl. Pharmacol. v.77 Comparative toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in four (sub) strains of adult male rats. Walden, R.;Schiller, C.M.
  30. Botchier Pharmacol. v.29 Increased total and high density lipoprotein cholesterol with apoprotein changes resembling streptozotocin diabetes in trachlodibenzodioxin (TCDD) treated rats. Poli, A.;Franceschini, G.;Puglisi.;Sirtori, C.R.
  31. Toxicol. Appl. Pharmacol. v.78 Dose related effects 2,3,7,8-tetrachlorodibenzo-p-dioxin in C57BL/6J and DBA/2J mice. Chapman, D.E.; Schiller, C.M.
  32. Bidchem. Biophys. Res. Commun. v.118 TCDD causes reduction in the low density lipoprotein(LDL) receptor activities in the hepatic plasma membrane of the guinea pig and rat. Bombick, D.W.;Matsumura, F.;Madhukar, B.V.
  33. Toxicol. Appl. Pharmacol. v.83 Intrermediary metabolism of the mature rat following 2,3,7,8-tetrachlorodibenzo-p-dioxin treatment. Christian, B.J.;Menahan, L.A.;Perterson, R.E.
  34. J. Biol. Chem v.255 The role of high density lipoproteins in rat adrenal cholesterol metabolism and steroidogenesis. Gwynne, J.T.;Hess, B.
  35. Food Technol. v.23 Use of chitinous polymers in food-A challenge for food research and development. Knorr, D.
  36. Biosci. Biotech. Botcher. v.58 Decreasing effect of chitosan on the apparent digestibility by rats fed a high diet. Deuchi, K.;Kanauchi, O.;Imasato, Y.;Kobayashi, E.
  37. Furda I, eds. Unconventional Sources of Dietary Fiber ACS Symposium Series v.214 Aminopolysaccharides-their potential as dietary fiber. Furda, I.
  38. Br. J. Nutr. v.78 Broier chicken body weight, feed intake, plasma lipid and small-intestinal bile acid concentration in response to feeding chitosan and pectin. Razdan, A.;Pettersson, D.;Pettersson, J.
  39. Biosci. Biotech. Botcher. v.64 Effect of dietary chitosans with different viscosity on plasma lipid and lipid peroxidation in rats fed on a diet enriched with cholesterol. Chiang, M.T.;Yao, H.T.;Chen, H.C.
  40. Int. J. Obes. v.23 Reduction in fat storage during chitin-chitosan treatment in mice fed a high-fat diet. Han, L.K.;Kimura, Y.;Okuda, H.
  41. Banbury Report #18 Induction of lipid peroxidation and inhibition of glutathion peroxidase by TCDD. Stohs, S.J.;Hassan, M.Q.;Muurray, W.J.
  42. Free Rad. Biol. Med. v.9 Oxidative stress induced by 2,3,7,8- tetrachlorodibenzo-p-dioxin (TCDD). Stohs, S.J.